Nucleic acids symposium series (2004)最新文献_第9页

Promotion of triplex formation by 3'-amino-2'-O,4'-C-methylene bridged nucleic acid modification. 3'-氨基-2'-O,4'- c -亚甲基桥接核酸修饰促进三联体形成。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp080

Kiyomi Sasaki, S M Abdur Rahman, Norihiro Sato, Satoshi Obika, Takeshi Imanishi, Hidetaka Torigoe

引用次数: 1

Detection of enzymatic activities related to the tRNA splicing pathway in Methanosarcina acetivorans cell extract. 活性甲烷藻细胞提取物中tRNA剪接通路相关酶活性的检测。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp150

Yuichiro Nomura, Takashi Yokogawa, Shigeo Yoshinari, Yoh-ichi Watanabe, Satoshi Ohno, Kazuya Nishikawa

引用次数: 0

Chemical methods to study protein-nucleic acid interactions. 研究蛋白质与核酸相互作用的化学方法。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp022

Chuan He

引用次数: 0

Exciton-controlled fluorescence: application to hybridization-sensitive fluorescent DNA probe. 激子控制荧光:应用于杂交敏感的荧光DNA探针。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp025

Akimitsu Okamoto, Shuji Ikeda, Takeshi Kubota, Mizue Yuki, Hiroyuki Yanagisawa

{"title":"Exciton-controlled fluorescence: application to hybridization-sensitive fluorescent DNA probe.","authors":"Akimitsu Okamoto, Shuji Ikeda, Takeshi Kubota, Mizue Yuki, Hiroyuki Yanagisawa","doi":"10.1093/nass/nrp025","DOIUrl":"https://doi.org/10.1093/nass/nrp025","url":null,"abstract":"A hybridization-sensitive fluorescent probe has been designed for nucleic acid detection, using the concept of fluorescence quenching caused by the intramolecular excitonic interaction of fluorescence dyes. We synthesized a doubly thiazole orange-labeled nucleotide showing high fluorescence intensity for a hybrid with the target nucleic acid and effective quenching for the single-stranded state. This exciton-controlled fluorescent probe was applied to living HeLa cells using microinjection to visualize intracellular mRNA localization. Immediately after injection of the probe into the cell, fluorescence was observed from the probe hybridizing with the target RNA. This fluorescence rapidly decreased upon addition of a competitor DNA. Multicoloring of this probe resulted in the simple simultaneous detection of plural target nucleic acid sequences. This probe realized a large, rapid, reversible change in fluorescence intensity in sensitive response to the amount of target nucleic acid, and facilitated spatiotemporal monitoring of the behavior of intracellular RNA.","PeriodicalId":87448,"journal":{"name":"Nucleic acids symposium series (2004)","volume":" 53","pages":"49-50"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/nrp025","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28396108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Multiple activities of c-di-GMP in Pseudomonas aeruginosa. c-二- gmp在铜绿假单胞菌中的多重活性。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp026

Stephen Lory, Massimo Merighi, Mamoru Hyodo

{"title":"Multiple activities of c-di-GMP in Pseudomonas aeruginosa.","authors":"Stephen Lory, Massimo Merighi, Mamoru Hyodo","doi":"10.1093/nass/nrp026","DOIUrl":"https://doi.org/10.1093/nass/nrp026","url":null,"abstract":"Survival strategies of many bacterial pathogens, including Pseudomonas aeruginosa, are linked to their ability to form surface associated communities called biofilms. The biofilm life style allows these organisms to persist in various tissues, avoid clearance by innate host defences and significantly enhanced their resistance to antibiotics. Formation of various biofilm components, including the synthesis of the extracellular polysaccharide matrix, is controlled at the transcriptional and translational levels and also by a small molecule second messenger bis-(3',5')-cyclic-di-guanidine monophosphate (c-di-GMP). The synthesis of c-di-GMP from GTP and its degradation is controlled by diguanylate cyclases (DGCs) and phosphodiesterases (PDEs), encoded by over thirty genes in the P. aeruginosa genome. We have shown that an increase in the intracellular c-di-GMP levels favors biofilm formation due to its role as a cofactor for the synthesis of several types of extracellular polysaccharides, including PEL and alginate, the two key virulence factors of P. aeruginosa during infection of patients with cystic fibrosis. During biosynthesis of PEL and alginate, c-di-GMP binds to specific receptors, PelD and Alg44, respectively. We have also recently demonstrated that DGCs have a relaxed specificity and can cyclize other nucleotides besides GTP. These atypical cyclic dinucleotides bind c-di-GMP receptors with high affinity, suggesting that intracellular regulation of various biological functions by this group of second messengers may be more complex than previously recognized.","PeriodicalId":87448,"journal":{"name":"Nucleic acids symposium series (2004)","volume":" 53","pages":"51-2"},"PeriodicalIF":0.0,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1093/nass/nrp026","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"28396109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 34

DNA junction structure stabilized by molecular crowding conditions. 分子拥挤条件下的DNA结结构稳定。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp030

Daisuke Miyoshi, Sanjukta Muhuri, Kenta Mimura, Naoki Sugimoto

引用次数: 2

DNA ligation using photoremovable protecting groups. 使用可光移保护基团的DNA连接。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp087

Yuta Kawano, Tadao Takada, Mitsunobu Nakamura, Kazushige Yamana

引用次数: 1

Handy and prompt DNA separation using PNA with internal disulfide bond. 使用具有内部二硫键的PNA进行方便快捷的DNA分离。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp088

Yuichiro Aiba, Makoto Komiyama

引用次数: 1

Electronic structure and UV absorption spectra of metal-mediate DNA: an approach from theoretical chemistry. 金属介导DNA的电子结构和紫外吸收光谱:一种理论化学方法。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp091

Toru Matsui, Hideaki Miyachi, Yasuyuki Nakanishi, Yasuteru Shigeta, Yasutaka Kitagawa, Mitsutaka Okumura, Kimihiko Hirao

引用次数: 0

Characterization of heme coordination structure in heme-DNA complex possessing gaseous molecule as an exogenous ligand. 以气态分子为外源配体的血红素- dna复合物中血红素配位结构的表征。

Nucleic acids symposium series (2004) Pub Date : 2009-01-01 DOI: 10.1093/nass/nrp121

Kaori Saito, Yusuke Nakano, Hulin Tai, Shigenori Nagatomo, Hikaru Hemmi, Hajime Mita, Yasuhiko Yamamoto

引用次数: 12