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Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology最新文献

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[Morphogenesis and histochemical investigation of peltate glandular trichomes on Glycyrrhiza uralensis Fisch. leaves]. 甘草果皮腺毛的形态发生和组织化学研究。树叶)。
Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2007-12-01
Li Peng, Zheng Hai Hu
{"title":"[Morphogenesis and histochemical investigation of peltate glandular trichomes on Glycyrrhiza uralensis Fisch. leaves].","authors":"Li Peng,&nbsp;Zheng Hai Hu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Anatomical and histochemical investigation of the glandular trichomes on the leaves of Glycyrrhiza uralensis Fisch.were carried out by using scanning electronic microscopy (SEM), Light microscopy and fluorescence microscopy. Two types of peltate glandular trichomes can be distinguished: one occurred on the leaves abaxial and adaxial surface with a short stalk and the second on the calyx surface with a long stalk. Secretory trichomes started as outgrowth of epidermal cells, subsequent divisions gave rise to trichomes made up of a basal cell, stalk cells and a multicellular secretory head. The histochemical identification revealed that complex substance excreted by the secretory cells contained flavoniods, polysaccharides and lipophilic compounds. During the development of glandular trichomes, only mature trichomes with ten-celled secretory head or even more cells could be observed the flavonoids inside or in subcuticular space but before that stages the flavonoids were not found. The findings provided a scientific basis for understanding the flavonoids synthesis and biological function.</p>","PeriodicalId":87435,"journal":{"name":"Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology","volume":"40 6","pages":"395-402"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41066536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Factors affecting efficient plant regeneration from wheat mature embryos. 小麦成熟胚高效植株再生的影响因素。
Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2007-12-01
Yang Yu, Zhi Ming Wei
{"title":"Factors affecting efficient plant regeneration from wheat mature embryos.","authors":"Yang Yu,&nbsp;Zhi Ming Wei","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":87435,"journal":{"name":"Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology","volume":"40 6","pages":"443-50"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40841819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Expression profiles of the genes associated with the myocyte differentiation during rat liver regeneration. 大鼠肝脏再生过程中心肌细胞分化相关基因的表达谱。
Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2007-12-01
Li Feng Zhao, Ming Zhen Zhang, Cun Shuan Xu
{"title":"Expression profiles of the genes associated with the myocyte differentiation during rat liver regeneration.","authors":"Li Feng Zhao,&nbsp;Ming Zhen Zhang,&nbsp;Cun Shuan Xu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Myocytes are important parts of tissues and organs. To study the effects of myocyte differentiation-related genes on rat liver regeneration (LR) at transcriptional level, we obtained these genes through collecting the database data and retrieving the pertinent thesis, and detected the expression profiles of above-mentioned genes during LR using the Rat Genome 230 2.0 array. LR-associated genes were identified by comparing the discrepancy in gene expression changes between partial hepatectomy (PH) group and sham-operation (SO) group, by which 52 LR-associated genes were confirmed. They were classified into 5 groups based on time relevance, including 0.5-1h; 2-12h; 16h, 30h, 42h, 96h; 18-24h, 36h, 48-60h; 66-72h,120-168h, in which the numbers of up-regulation and down-regulation genes were 8 and 10, 24 and 8, 21 and 24, 53 and 64, 28 and 36, respectively. Among these genes, the total 143 times of up-regulation and 136 times of down-regulation, as well as their 8 expression patterns displayed diversity and complexity of the genes associated with the myocyte differentiation. It was inferred that the differentiation of myoblasts and smooth muscle cells were enhanced during LR and the genes associated with the differentiation of skeletal muscle cells and cardiac muscle cells participated in the cellular physiological and biochemical activities of LR.</p>","PeriodicalId":87435,"journal":{"name":"Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology","volume":"40 6","pages":"387-94"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41066535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Some effects of the neodymium ion on the cell wall structure of Staphyloccocus aruea]. [钕离子对葡萄球菌细胞壁结构的影响]。
Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2007-12-01
Guang Hua Huo, Dong Yan Zhang, Tong Zhang
{"title":"[Some effects of the neodymium ion on the cell wall structure of Staphyloccocus aruea].","authors":"Guang Hua Huo,&nbsp;Dong Yan Zhang,&nbsp;Tong Zhang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The aim of this research is to study the effect of neodymium ion on the cell wall structure of Staphyloccocus aruea using transmission electron microscope, amino acid analyzer, infrared absorption spectrometry (IR). Experimental results show that Nd3+ can change the shape and the structure of the cell wall of Staphyloccocus aruea NdCl3 with lower concentration than the bacteriostatic concentration can help to synthesize the cell wall structure of Staphyloccocus aruea. Nd3+ with higher concentration than the bacteriostatic concentration can break the peptide bond and the hydrogen bond so as to break the net structure in the peptideoglycan cell.</p>","PeriodicalId":87435,"journal":{"name":"Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology","volume":"40 6","pages":"437-42"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40841818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genetic polymorphisms and genetic effects of CAST gene in pigs. 猪CAST基因的遗传多态性及遗传效应。
Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2007-12-01
Hui Liang Xue, Lai Xiang Xu
{"title":"Genetic polymorphisms and genetic effects of CAST gene in pigs.","authors":"Hui Liang Xue,&nbsp;Lai Xiang Xu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Single-nucleotide polymorphisms of the porcine CAST gene were tested using PCR-SSCP. Genetic effects of the CAST gene on the meat quality and the backfat thickness were analyzed. On the basis of the published cDNA sequence (M20 160) of the porcine CAST gene, seven pairs of primers were designed, and two polymorphisms were found in the PCR products amplified with F1/ R1, and F6/R6 primers. A --> G transition at 317 site, G --> C transition at 2042 site were detected by sequencing the homozygotes. The results show that: Firstly, the Landrace, the Large White, and the Duroc breeds differ significantly (P < 0.01) in haplotype distribution from the Laiwu and the Yimeng breeds. Secondly, on the basis of the fixed effect model, significant differences (P < 0.05) were found in the tenderness and the backfat thickness among the different CAST haplotypes, whereas no significant differences (P > 0.05) existed in the pH, the temperature and the drip loss. Thirdly, using least square analysis, it was seen that significant differences (P < 0.05) were found in the temperature at 12 h and 24 h postmortem, the pH at 30 min and 1 h postmortem and drip loss among different pig breeds. Individuals of the BBCC and BBDD haplotypes had significantly less (P < 0.01) tenderness than those of the other haplotypes, and the pigs of the AACC and AADD haplotypes had significantly higher (P < 0.01) backfat thickness than those of the other haplotypes. These results suggest that the genotype has significant effects on the meat quality and the backfat thickness. CAST can be used in marker-assisted selection to provide significant improvements for meat quality and to accelerate the breeding progress.</p>","PeriodicalId":87435,"journal":{"name":"Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology","volume":"40 6","pages":"403-9"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41066537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Embryogenesis of microspore derived multicells in Capsicum annuum L]. 辣椒小孢子衍生多细胞的胚胎发生[j]。
Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2007-12-01
Fan Liu, Hong Zhao, Bin Chen, Yue Yun Zhang
{"title":"[Embryogenesis of microspore derived multicells in Capsicum annuum L].","authors":"Fan Liu,&nbsp;Hong Zhao,&nbsp;Bin Chen,&nbsp;Yue Yun Zhang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Microspores and derived multicells were isolated and cultured in modified liquid CP medium after a 15d's preculture of anthers on solidified medium. Thirty days later in suspension culture, at 28 degrees C dark condition embryoids with different developmental stages were formed. Up to 22 embryoids could be formed from the cell suspension of 12 anthers, and about 23% of the embryoids were at the cotyledonary stage. Fluorescence and light microscope observations revealed that these embryoids derived from microspores. After several symmetrical division of the nuclei of uninucleated microspores, multi-nuclei cells or multi-cells were formed, and developed further into embryoids. There were white hairs on the surface of pepper embryoids, and some embryoids showed low vigor while others showed normal by TTC staining. Plants could be formed from torpedo and cotyledonary stage embryoids on solidified medium. Embryoids could be induced by 7 degrees C, 32 degrees C or 35 degrees C stress treatment on anthers, Higher embryogenesis frequencies were got at 7 degrees C and 35 degrees C condition in anther culture while 35 degrees C and 32 degrees C treatment showed a higher embryogenesis in isolated multicell culture. The reason of this result was discussed. There were obvious differences in embryogenesis frequency among different genotypes and different temperature stress conditions. Flow cytometric analysis revealed that there were haploidy, doubled haploidy and haploid-diploid chimera in the regenerated plants.</p>","PeriodicalId":87435,"journal":{"name":"Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology","volume":"40 6","pages":"371-9"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41066533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Different proteins in mitochondrial proteome of T-type maize cytoplasmic male-sterile line and its maintainer line]. [t型玉米细胞质雄性不育系及其保持系线粒体蛋白质组的不同蛋白]。
Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2007-12-01
Wei Jia Zhang, Chun Zheng Li, Hai Quan Huang, Zhen Ying Wang, Yong Kang Peng
{"title":"[Different proteins in mitochondrial proteome of T-type maize cytoplasmic male-sterile line and its maintainer line].","authors":"Wei Jia Zhang,&nbsp;Chun Zheng Li,&nbsp;Hai Quan Huang,&nbsp;Zhen Ying Wang,&nbsp;Yong Kang Peng","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Using T-type maize cytoplasmic male sterile line (T-CMS) and maintainer line as experimental materials, we separated mitochondrial proteins from leaves at seedling, shooting, booting stages, mesocotyl, root and anther at meiosis of pollen mother cell, single-double nucleus stage of pollen grain by two-dimensional electrophoresis with immobilized pH3-10 gradients. About 150 mitochondrial protein spots in seedling leaves, 150 spots in mesocotyls, 150 spots in roots and 100 spots in meiosis anther were observed respectively in this investigation. 6 difference protein spots were identified by MALDI-TOF-MS analysis and NCBI database searching. r40c1 protein was present in mesocotyl of T-CMS and absent in maintainer line. Mature anther-specific protein, DNA-directed RNA polymerase 23kDa subunit, hexokinase II were present and glutathione S-transferase, putative polyprotein were absent in pollen aborted anther of T-CMS. Developmental changes in mitochondrial proteins were found in leaves but no differences were observed in T-CMS and its maintainer line. Obvious differences of mitochondrial proteins were found at single-double nucleus stage anther in T-CMS and maintainer line. These different proteins were considered to be associated to pollen aborted in T-CMS.</p>","PeriodicalId":87435,"journal":{"name":"Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology","volume":"40 6","pages":"410-8"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41066538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The structural characteristics and symplastic transport function of the Ectodesmata-like of root cap tissue in Zea mays]. [玉米根冠外丝样组织的结构特征及共塑运输功能]。
Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2007-10-01
Zhong He Wang, Na Liu, Gang Liu, Dong Mei Wang
{"title":"[The structural characteristics and symplastic transport function of the Ectodesmata-like of root cap tissue in Zea mays].","authors":"Zhong He Wang,&nbsp;Na Liu,&nbsp;Gang Liu,&nbsp;Dong Mei Wang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The ultrastructure and symplastic transport function of Ectodesmata-like (ED-like) of the root cap cells of Zea mays, during the detaching stage, were reported by using fluorescence and electron microscopy. It was described the process that plasmodesmata (PD) were gradually stretched and changed into ED-like. It was discovered that the diameter of appressed endoplasmic reticulum (AER) in PD became thinner while the ED-like still remained some structures of PD. By using fluorescence probe incubating, 457Da Lucifer Yellow (LYCH) which was impermeable to the membrane, could enter the root cap cells through ED-like. The results proved that ED-like still retained physiological activity and kept the symplastic transport function during a period time. When the root tissues were pre-treated by cytochalasin D (CD), Phalloidin and 2,3-butanedione, 2-monoxime (BDM) and then combined with fluorescence probe detecting, the results showed that F-actin and myosin might take part in the regulation of the substance translocation of the ED-like.</p>","PeriodicalId":87435,"journal":{"name":"Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology","volume":"40 5","pages":"351-8"},"PeriodicalIF":0.0,"publicationDate":"2007-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27247279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Cloning, antisense construction and tomato transformation of Lecop1like gene]. [lecop1样基因的克隆、反义构建及番茄转化]。
Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2007-10-01
Meng Xiang Cui, Peng Li Li, Zhong Qi Liu, Feng Jin, Li Chang, Xiao Xing Feng, Ning Ning Wang
{"title":"[Cloning, antisense construction and tomato transformation of Lecop1like gene].","authors":"Meng Xiang Cui,&nbsp;Peng Li Li,&nbsp;Zhong Qi Liu,&nbsp;Feng Jin,&nbsp;Li Chang,&nbsp;Xiao Xing Feng,&nbsp;Ning Ning Wang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Here reported a 1060 bp cDNA cloning of LeCOP1LIKE gene by EST probing and RT-PCR method. In order to characterize function of this gene, a LeCOP1LIKE antisense expression vector was transformed into Micro-Tom via Agrobacterium-mediated transformation method and 10 independent transgenic lines were obtained. RT-PCR analysis showed that the expression of LeCOP1LIKE gene was evidently repressed in 4 lines of them. The transgenic plants were much shorter than their wild type control, their chlorophyll content was increased but the seed development was obviously suppressed. All these results suggested that the cloned LeCOP1LIKE gene was a negative regulator of photomorphogenesis in tomato.</p>","PeriodicalId":87435,"journal":{"name":"Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology","volume":"40 5","pages":"329-38"},"PeriodicalIF":0.0,"publicationDate":"2007-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27247411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[The localization of galanthamine in vegetative organ of Lycoris aurea Herb]. [加兰他敏在石蒜营养器官中的定位]。
Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2007-10-01
Xiao Yan Wang, Peng Kai Wang, Min Ren Huang, Jian Zhou, Zheng Min Han
{"title":"[The localization of galanthamine in vegetative organ of Lycoris aurea Herb].","authors":"Xiao Yan Wang,&nbsp;Peng Kai Wang,&nbsp;Min Ren Huang,&nbsp;Jian Zhou,&nbsp;Zheng Min Han","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Using laser confocal microscopical techniques, we observed the anatomical structure of mature root, bulb, and leaf of Lycoris aurea Herb., and also did some research on the localization of galanthamine in the above-mentioned vegetative organ. The results are as follows: In the mature root, the galanthamine distributes mainly in cell wall, especially in cell wall of exodermis and endodermis and vessel wall. In the mature leaf, galanthamine exist in cell wall of vascular bundle, mesophyll cell between vascular bundles and epidermis cells. The scale leaf is the essential accumulational organ. Plenty of galanthamine distribute in the adaxial parenchyma cell, epidermis cell wall, and also in the clingy cell of abaxial epidermis cell.</p>","PeriodicalId":87435,"journal":{"name":"Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology","volume":"40 5","pages":"339-45"},"PeriodicalIF":0.0,"publicationDate":"2007-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27247412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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