Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology最新文献_第8页

[Calcium distribution in the central cell of lettuce (Lactuca sativa L.) before and after pollination]. [授粉前后莴苣（Lactuca sativa L.）中心细胞中的钙分布]。

Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2008-02-01

Yi Lan Qiu, Ru Shi Liu, Lv Ye, Hui Tian

{"title":"[Calcium distribution in the central cell of lettuce (Lactuca sativa L.) before and after pollination].","authors":"Yi Lan Qiu, Ru Shi Liu, Lv Ye, Hui Tian","doi":"","DOIUrl":"","url":null,"abstract":"Potassium antimonite precipitation was used to locate calcium in the central cell of lettuce (Lactuca sativa L.) before and after pollination. At 3d before anthesis, two polar nuclei of central cell separately located at two polarity of the cell, and few calcium precipitates (ppts) appeared in the polar nuclei and cytoplasm, but some ppts in its small vacuoles. At 2d before anthesis, two polar nuclei moved toward the middle of the cell and fused to form a secondary nucleus, and the ppts evidently increased in the nucleus and cytoplasm. At 1d before anthesis, secondary nucleus again moved toward micropylar end and located near the egg to prepare for fertilization. Calcium precipitates were mainly accumulated in the secondary nucleus. After pollination and before fertilization, the distribution of calcium ppts was similar to that before pollination. At 4h after pollination, the central cell was fertilized, and calcium ppts evidently increased in the cell and numerous were accumulated in its nucleus and cytoplasm. At 6h after pollination, the primary endosperm nucleus completed its first division and formed two dissociate endosperm nuclei, and still many calcium precipitates appeared in the nucleus and cytoplasm. With endosperm development, calcium ppts decreased in the endosperm cell. At 1d after emasculated and without pollination, the secondary nucleus of the cell still bordered on the egg and some calcium ppts appeared in the secondary nucleus. The results indicated that the temporal and spatial changes of calcium in the central cell may play an important physiological role during the development of the central cell and endosperm.","PeriodicalId":87435,"journal":{"name":"Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology","volume":"41 1","pages":"61-9"},"PeriodicalIF":0.0,"publicationDate":"2008-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27428228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Chromosomal studies of germ cells in diploid and polyploid fish produced by distant crossing]. 远距离杂交产生的二倍体和多倍体鱼类生殖细胞的染色体研究。

Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2008-02-01

Chun Zhang, Shao Jun Liu, Yuan Dong Sun, Jun Xiao, Qin Bo Qin, Jing Wang, Wei Guo He, Cui Ping You, Yun Liu

{"title":"[Chromosomal studies of germ cells in diploid and polyploid fish produced by distant crossing].","authors":"Chun Zhang, Shao Jun Liu, Yuan Dong Sun, Jun Xiao, Qin Bo Qin, Jing Wang, Wei Guo He, Cui Ping You, Yun Liu","doi":"","DOIUrl":"","url":null,"abstract":"By observing the chromosomal spreads of germ cells and tissue sections, we studied the chromosomal spreads of germ cells in diploid and polyploid fish produced by distant crossing. The samples covered the second generation of hybrids of red crucian carp (Carassius auratus red var.) (female) x common carp (Cyprinus carpio) (male) (2n = 100) (F2), allotetraploid hybrids of red crucian carp (female) x common carp (male)(4n = 200), triploid hybrids of gold fish (female)x allotetraploid (male) (3n = 150), the second generation of the gynogenetic progeny of allotetraploid hybrids (G2) (2n = 100), and the common carp (2n = 100) used as a control. The results demonstrated that chromosomal number of spermotogonia in common carp was equal to that of their somatic cell (2n = 100), while the chromosomal number of germ cells in diploid hybrid fish and polyploid hybrid fish had doubled obviously, and the frequent of chromosomal doubling in spermotogonia of F2 appeared especially high, accounting for 21.6% of all examined chromosomal spreads, which provided directly cytological evidence for the production of unreduced diploid gametes in F2 and also indicated that distant crossing was an important factor to lead to chromosomal doubling in germ cell. This research had important significance in studies on the formation of polyploidy fish and fish breeding.","PeriodicalId":87435,"journal":{"name":"Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology","volume":"41 1","pages":"53-60"},"PeriodicalIF":0.0,"publicationDate":"2008-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27428227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Expression of glutathione S-transferase-aquaporin 1 carboxyl terminal domain fusion protein in Escherchia coli]. 谷胱甘肽s -转移酶-水通道蛋白1羧基末端结构域融合蛋白在大肠杆菌中的表达

Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2008-02-01

Xiao Meng Li, Jiang Li, Nan Yang Yang, Xin Gang Guan, Shu Zhi Zhang, Wei Li Hui, Tong Hui Ma

引用次数: 0

[Cotton laccase gene overexpression in transgenic Populus alba var. pyramidalis and its effects on the lignin biosynthesis in transgenic plants]. 棉花漆酶基因在转基因白杨中的过表达及其对木质素合成的影响[j]。

Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2008-02-01

Ji Wang, Mu Lan Zhu, Zhi Ming Wei

引用次数: 0

[Distributional difference of fat-soluble compounds in the roots, stems and leaves of four Salvia plants]. [四种鼠尾草植物根、茎、叶中脂溶性化合物的分布差异]。

Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2008-02-01

Jin Tong Li, Juan E Dong, Zong Suo Liang, Zhi Ming Shu, Guo Wei Wan

{"title":"[Distributional difference of fat-soluble compounds in the roots, stems and leaves of four Salvia plants].","authors":"Jin Tong Li, Juan E Dong, Zong Suo Liang, Zhi Ming Shu, Guo Wei Wan","doi":"","DOIUrl":"","url":null,"abstract":"To illustrate distribution of fat-soluble compounds in the roots, stems and leaves of four Salvia plants, the methods of Histochemistry and HPLC were adopted to analyze different parts of the four Salvia plants in this paper. The results showed that distribution was differential, and following as this: the roots, stems and leaves of four Salvia plants contained fat-soluble compounds, moreover, the fat-soluble compounds of the roots located in periderm and the stems and leaves in epidermis. The main components of the fat-soluble compounds were Tanshinone IIA, Tanshinone I and Dihydrotanshinone I in the toots of Salvia miltiorrhiza Bunge and Salvia miltiorrhiza bge. f. alba, yet there were only Tanshinone IIA in the roots of Salvia japonica and Salvia officinalis. And fat-soluble compounds were not Tanshinone IIA, Tanshinone I and Dihydrotanshinone I in the stems and leaves of four Salvia plants. The type and content of fat-soluble compounds related to the species and introduction regions, they changed with the species and introduction regions. The conclusion clarified the accurate distribution of fat-soluble compounds in the different parts of four Salvia plants, and provided some theoretical basis for the application of Chinese herbs.","PeriodicalId":87435,"journal":{"name":"Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology","volume":"41 1","pages":"44-52"},"PeriodicalIF":0.0,"publicationDate":"2008-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27428226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Production of porcine reconstructed embryos by whole-cell intracytoplasmic microinjection]. 全细胞胞浆内显微注射制备猪重构胚胎。

Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2008-02-01

Qing Ran Kong, Yi Bo Luo, Jiang Tian Tian, Zhen Kun Wang, Li Zhang, Zhong Hua Liu

{"title":"[Production of porcine reconstructed embryos by whole-cell intracytoplasmic microinjection].","authors":"Qing Ran Kong, Yi Bo Luo, Jiang Tian Tian, Zhen Kun Wang, Li Zhang, Zhong Hua Liu","doi":"","DOIUrl":"","url":null,"abstract":"Cloning by somatic cell nuclear transfer has been achieved by both electric fusion and intracytoplasmic nuclear injection (ICNI) methods. However, each of the above methods involves extended complicate manipulation and special equipment. Here we report a whole-cell injection technique without Piezo assistance for nuclear transfer in pigs. The fibroblast cell of pig as the nucleus donor cell, effects of the new method on the efficiency of somatic nuclear transfer in pig were investigated, compared with that of electric fusion method. Results showed that the new method was a little less efficient in producing reconstructed embryos but without significant difference (88.4% vs 78%, P > 0.05). After the embryos were cultured 48h and 7d, the fusion method is more efficient than the new method in the oocyte cleavage rate and the blastocyst development (78% vs 53.2%, P < 0.05; 27.2% vs 13.8%, P < 0.01). The results indicate that both methods make no difference in the quality of the blastula, but the electric fusion method is more efficient. Therefore, the applicability of producing normal,cloned piglets by the simple and less labor-intensive whole-cell intracytoplasmic injection needs further improvement.","PeriodicalId":87435,"journal":{"name":"Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology","volume":"41 1","pages":"70-4"},"PeriodicalIF":0.0,"publicationDate":"2008-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27428229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Anther development and sperm isolation of Pseudostellaria heterophylla (Miq.)]. 异叶伪星(Pseudostellaria heterophylla, Miq.)花药发育与精子分离

Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2007-12-01

Zu Yun Ye, Hua Deng, Ming Xia Jian, Song Zheng, Hui Qiao Tian

引用次数: 0

[Cytochemical observation on the developing anthers of Allium cepa L]. [葱属植物花药发育的细胞化学观察]。

Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2007-12-01

Dong Mei Wei, Ming Xia Jian, Hua Deng, Song Zheng, Hui Qiao Tian

{"title":"[Cytochemical observation on the developing anthers of Allium cepa L].","authors":"Dong Mei Wei, Ming Xia Jian, Hua Deng, Song Zheng, Hui Qiao Tian","doi":"","DOIUrl":"","url":null,"abstract":"Polysaccharides was detected using the periodic-acid-Schiff's (PAS) technique and lipid detected using Sudan black during the anther development of Allium cepa L. Before the meiosis of microspore mother cells there were a few lipid drops in endothecium cells and little polysaccharides in tapetal cells which did not differentiate completely in young anthers. At the stage of tetrad there were still few polysaccharides and lipid material in young anther, and only cell wall of anther wall and callose wall of tetrads displayed the feature of polysaccharids. The size of tapetal cells began to increase at this stage. During microspore development the tapetal cells reached its maximal size, and many lipid drops were accumulated in the cells. However, few lipid drops and starches appeared in microspores. At early stage of 2-celled pollen, the vegetative cell of 2-celled pollen began to accumulate starches. Tapetal cells degenerated at this stage and its lipid drops concentrated to form lipid block. Then the starches in 2-celled pollen disappeared with pollen development, and many lipid drops were accumulated in vegetative cell of nearly mature pollen.","PeriodicalId":87435,"journal":{"name":"Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology","volume":"40 6","pages":"451-7"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40841820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[The expression of galanin and galanin receptor-2 in the brain of chronic stress model of depression]. 慢性应激抑郁症大鼠脑组织中甘丙肽及其受体-2的表达

Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2007-12-01

Yi Lei Ye, Yan Ting Qi, Liang Shan, Li Juan Zou, Liang Cai Gao, Chong Gang Yuan

引用次数: 0

[Hypermethylation in promoter area of LDLR gene in atherosclerosis patients]. 【动脉粥样硬化患者LDLR基因启动子区高甲基化】。

Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology Pub Date : 2007-12-01

Yan Fang Zhi, Yan Sheng Huang, Zhu Hua Li, Rui Ming Zhang, Shu Ren Wang

{"title":"[Hypermethylation in promoter area of LDLR gene in atherosclerosis patients].","authors":"Yan Fang Zhi, Yan Sheng Huang, Zhu Hua Li, Rui Ming Zhang, Shu Ren Wang","doi":"","DOIUrl":"","url":null,"abstract":"To investigate the methylation pattern of 12 CpG dinucleotide sites in promoter region of LDLR gene in atherosclerosis patients, peripheral blood DNA samples were prepared from 61 atherosclerosis patients and 28 healthy subjects. The methylation status of CpG islands in LDLR gene promoter region was measured by using a modified coordinative method with nested-PCR, Touchdown-PCR and methylation-sensitive-single-strand conformation analysis (MS-SSCA). Three types of methylation patterns were detected in promoter region of LDLR gene in peripheral blood genome of atherosclerosis patients: full-methylation, part-methylation and none-methylation. 2 cases were full-methylation and 13 cases were part-methylation, the methylation frequency was 24.59% in atherosclerosis patients. While in 28 healthy control, only 1 half-methylation sample was found, the methylation frequency was 3.57%, which is much lower than that in atherosclerosis patients (P < 0.05). A significantly higher methylation degree in promoter region of LDLR gene was found in peripheral blood genome of atherosclerosis patients compared with the controlled healthy subjects, which may suggest an involvement of aberrant methylation of LDLR gene in initiation and development of AS. The modified coordinative method with nested-PCR, Touchdown-PCR and MS-SSCA manifests merits of convenience, cheap and high efficiency, which lowers the requirement for the DNA template and increases the sensitivity and specificity.","PeriodicalId":87435,"journal":{"name":"Fen zi xi bao sheng wu xue bao = Journal of molecular cell biology","volume":"40 6","pages":"419-27"},"PeriodicalIF":0.0,"publicationDate":"2007-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41066539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0