IEE proceedings. Nanobiotechnology最新文献_第8页

Biomolecule-compatible support structures for biomolecule coupling to physical measuring principle surfaces. 用于生物分子与物理测量原理表面耦合的生物分子兼容支撑结构。

IEE proceedings. Nanobiotechnology Pub Date : 2004-06-01 DOI: 10.1049/ip-nbt:20040691

A Jung, P Berlin, B Wolters

{"title":"Biomolecule-compatible support structures for biomolecule coupling to physical measuring principle surfaces.","authors":"A Jung, P Berlin, B Wolters","doi":"10.1049/ip-nbt:20040691","DOIUrl":"https://doi.org/10.1049/ip-nbt:20040691","url":null,"abstract":"As part of studies on biomolecule-compatible interfacial structures for practice-relevant biosensor and biochip developments, new film-forming aminocelluloses of the 'P-CH2-NH-(X)-NH2' type (P = cellulose) with spacer structures (X = special oligoamine residues) at C6 and solubilising groups (S = tosylate or carbanilate) at C2C3 of the anhydroglucose unit (AGU) were synthesised and their film properties and covalent coupling with enzyme protein examined. Depending on the nature and degree of substitution (DS(S)) of the ester groups (S) at C2C3, the new aminocellulose derivatives are soluble either in DMA and DMSO (with S = carbanilate) or in water (with S = tosylate). The aminocellulose derivatives form transparent films from their solutions. AFM investigations of the film surfaces have either shown very flat (topography <1 nm) films or tubular topographies of nanostructure size, depending on structural and environment-induced factors of influence. Especially in the case of films from water-soluble aminocelluloses with oligoamine residues at C6, inter alia, enzyme-specific pH values and different positive charge distributions can be adjusted by partial protonation of the NH2 end groups. By means of the covalent coupling of the new aminocelluloses with glucose oxidase (GOD) it was shown that the enzyme coupling efficiency can be decisively optimised by the interplay of aminocellulose structure, coupling structure and enzyme protein.","PeriodicalId":87402,"journal":{"name":"IEE proceedings. Nanobiotechnology","volume":"151 3","pages":"87-94"},"PeriodicalIF":0.0,"publicationDate":"2004-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1049/ip-nbt:20040691","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25853704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Formation of focal adhesion-stress fibre complexes coordinated by adhesive and non-adhesive surface domains. 黏附和非黏附表面结构域协调的黏附应力纤维复合物的形成。

IEE proceedings. Nanobiotechnology Pub Date : 2004-04-01 DOI: 10.1049/ip-nbt:20040474

B Zimerman, M Arnold, J Ulmer, J Blümmel, A Besser, J P Spatz, B Geiger

{"title":"Formation of focal adhesion-stress fibre complexes coordinated by adhesive and non-adhesive surface domains.","authors":"B Zimerman, M Arnold, J Ulmer, J Blümmel, A Besser, J P Spatz, B Geiger","doi":"10.1049/ip-nbt:20040474","DOIUrl":"https://doi.org/10.1049/ip-nbt:20040474","url":null,"abstract":"Cell motility consists of repeating cycles of protrusion of a leading edge in the direction of migration, attachment of the advancing membrane to the matrix, and pulling of the trailing edge forward. In this dynamic process there is a major role for the cytoskeleton, which drives the protrusive events via polymerisation of actin in the lamellipodium, followed by actomyosin contractility. To study the transition of the actin cytoskeleton from a 'protrusive' to 'retractive' form, we have monitored the formation of focal adhesions and stress fibres during cell migration on a micro-patterned surface. This surface consisted of parallel arrays of 2 microm-wide, fibronectin-coated gold stripes, separated by non-adhesive (poly(ethylene glycol)-coated) glass areas with variable width, ranging from 4-12 microm. Monitoring the spreading of motile cells indicated that cell spreading was equally effective along and across the adhesive stripes, as long as the non-adhesive spaces between them did not exceed 6 microm. When the width of the PEG region was 8 microm or more, cells became highly polarised upon spreading, and failed to reach the neighboring adhesive stripes. It was also noted that as soon as the protruding lamella successfully crossed the PEG-coated area and reached an adhesive region, the organisation of actin in that area was transformed from a diffuse meshwork into a bundle, oriented perpendicularly to the stripes and anchored at its ends in focal adhesions. This transition depends on actomyosin-based contractility and is apparently triggered by the adhesion to the rigid fibronectin surface.","PeriodicalId":87402,"journal":{"name":"IEE proceedings. Nanobiotechnology","volume":"151 2","pages":"62-6"},"PeriodicalIF":0.0,"publicationDate":"2004-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1049/ip-nbt:20040474","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25853699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 37

Novel molecular device based on electrostatic interactions in organic polymers. 基于有机聚合物中静电相互作用的新型分子器件。

IEE proceedings. Nanobiotechnology Pub Date : 2004-04-01 DOI: 10.1049/ip-nbt:20040442

H L Kwok, J B Xu

{"title":"Novel molecular device based on electrostatic interactions in organic polymers.","authors":"H L Kwok, J B Xu","doi":"10.1049/ip-nbt:20040442","DOIUrl":"https://doi.org/10.1049/ip-nbt:20040442","url":null,"abstract":"A number of researchers have reported attempts to design molecular level devices. One approach is to make use of electrostatic interactions in different parts of a polymeric molecule. This paper reports a means to achieve this by adding space charge to a molecule consisting of symmetric and asymmetric subgroups. Physically, space charge residing in a subgroup produces a dipolar charge layer thereby creating a potential trough in the polymer backbone. By lifting or lowering this potential minimum, it is possible to modify the terminal current. The effect of space charge on the potential profile in the polymer backbone was examined and the change correlated to data on carrier mobilities for OC1C10-PPV reported in the literature. Modulation of space charge in the subgroup allows the manipulation of current flow along the polymer backbone, forming the basis for the development of a molecular device. A first-order analysis suggested that such a device could have current-voltage (I-V) characteristics similar to those of a MOSFET at subthreshold, with an estimated transconductance approximately 1-2 pAV and a cutoff frequency approximately 10(15) Hz.","PeriodicalId":87402,"journal":{"name":"IEE proceedings. Nanobiotechnology","volume":"151 2","pages":"48-52"},"PeriodicalIF":0.0,"publicationDate":"2004-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1049/ip-nbt:20040442","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25854384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cell response to nano-islands produced by polymer demixing: a brief review. 聚合物脱混产生纳米岛的细胞反应：综述。

IEE proceedings. Nanobiotechnology Pub Date : 2004-04-01 DOI: 10.1049/ip-nbt:20040534

M J Dalby, D Pasqui, S Affrossman

引用次数: 0

Nanoscale surface engineered living cells with extended substrate spectrum. 扩展底物光谱的纳米级表面工程活细胞。

IEE proceedings. Nanobiotechnology Pub Date : 2004-04-01 DOI: 10.1049/ip-nbt:20040535

W C Mak, K W Sum, D Trau, R Renneberg

引用次数: 10

Scanning probe technology in metalloprotein and biomolecular electronics. 扫描探针技术在金属蛋白和生物分子电子学中的应用。

IEE proceedings. Nanobiotechnology Pub Date : 2004-04-01 DOI: 10.1049/ip-nbt:20040504

J J Davis, D A Morgan, C L Wrathmell, A Zhao

{"title":"Scanning probe technology in metalloprotein and biomolecular electronics.","authors":"J J Davis, D A Morgan, C L Wrathmell, A Zhao","doi":"10.1049/ip-nbt:20040504","DOIUrl":"https://doi.org/10.1049/ip-nbt:20040504","url":null,"abstract":"The interfacing of man-made electronic components with specifically-folded biomacromolecules lies central not only to the development of sensory interfaces and potential new molecular-scale devices, but also enables us to analyse processes of great biological importance in a refined and controllable manner. Recent advances in both available technology, most notably optical and scanning probes in nature, and our understanding of suitable methodologies, have led us to the point where the characteristics of single biological molecules can be interrogated with good levels of reproducibility. We review here the application of scanning probe microscopy to the analysis of and experimentation on biological redox systems. Within this paper the tunnel transport characteristics, as assayed by both scanning tunnelling microscopy (STM) and conducting probe atomic force microscopy (AFM), of single metalloproteins are discussed. In a specific case study the electron transfer characteristics of the blue copper metalloprotein, azurin, are reported. The modulation of these properties under the influence of calibratable compressional force has also been examined in some detail. Work such as this enables one to reproducibly establish the conductance, barrier height, environmental sensitivity and electromechanical properties of these molecules.","PeriodicalId":87402,"journal":{"name":"IEE proceedings. Nanobiotechnology","volume":"151 2","pages":"37-47"},"PeriodicalIF":0.0,"publicationDate":"2004-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1049/ip-nbt:20040504","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25854383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Analysis of cellular structure by light scattering measurements in a new cytometer design based on a liquid-core waveguide. 基于液芯波导的新型细胞仪设计中的细胞结构光散射分析。

IEE proceedings. Nanobiotechnology Pub Date : 2004-03-15 DOI: 10.1049/ip-nbt:20060007

K. Singh, C. Liu, C. Capjack, W. Rozmus, C. Backhouse

引用次数: 11

Analysis of cellular structure by light scattering measurements in a new cytometer design based on a liquid-core waveguide. 基于液芯波导的新型细胞仪设计中的细胞结构光散射分析。

IEE proceedings. Nanobiotechnology Pub Date : 2004-02-01 DOI: 10.1049/ip-nbt:20031291

K Singh, C Liu, C Capjack, W Rozmus, C J Backhouse

引用次数: 0

Kv channel S6 helix as a molecular switch: simulation studies. Kv通道S6螺旋作为分子开关:模拟研究。

IEE proceedings. Nanobiotechnology Pub Date : 2004-02-01 DOI: 10.1049/ip-nbt:20040101

J N Bright, M S P Sansom

{"title":"Kv channel S6 helix as a molecular switch: simulation studies.","authors":"J N Bright, M S P Sansom","doi":"10.1049/ip-nbt:20040101","DOIUrl":"https://doi.org/10.1049/ip-nbt:20040101","url":null,"abstract":"Ion channels form pores of nanoscopic dimensions in biological membranes and play a key role in the physiology of cells. The majority of ion channels are gated, i.e. they contain a molecular switch that allows a transition between a closed (functionally 'off') and open (functionally 'on') state. Comparison of crystal structures of potassium channels suggest that the gating mechanism of voltage-gated potassium (Kv) channels involves a key role for the pore-lining S6 helix. There is a conserved PVP sequence motif in the S6 helix. Molecular dynamics simulations are used here to explore the conformational dynamics of the S6 helix hinge in models of fragments of a Kv channel, namely an S5-P-S6 monomer and an (S5-P-S6)4 tetramer. The latter is a model of the complete pore-forming domain of a Kv channel. All models were simulated embedded in an octane slab (a simple membrane mimetic). The results of these simulations indicate that the PVP motif may form a molecular hinge, even when the S6 helix forms part of a more complex model. The conformational dynamics of S6 are modulated by the remainder of protein, but it remains flexible. These simulation results are compatible with a channel gating model in which S6 bends in the vicinity of the PVP motif in addition to the region around the conserved glycine (G466) that is N-terminal to the PVP motif. This model is supported by comparison of the Kv S6 models with the S6 helix of the bacterial KvAP channel crystal structure. Thus, K channel gating may depend on a complex nanoswitch with three rigid helical sections linked by two molecular hinges.","PeriodicalId":87402,"journal":{"name":"IEE proceedings. Nanobiotechnology","volume":"151 1","pages":"17-27"},"PeriodicalIF":0.0,"publicationDate":"2004-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1049/ip-nbt:20040101","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25854381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 19

The future of computing--new architectures and new technologies. 计算的未来——新架构和新技术。

IEE proceedings. Nanobiotechnology Pub Date : 2004-02-01 DOI: 10.1049/ip-nbt:20030876

P Warren

引用次数: 9