IEE proceedings. Nanobiotechnology最新文献_第2页

Engineering challenges of BioNEMS: the integration of microfluidics, micro- and nanodevices, models and external control for systems biology. BioNEMS的工程挑战:集成微流体，微和纳米器件，模型和系统生物学的外部控制。

IEE proceedings. Nanobiotechnology Pub Date : 2006-08-01 DOI: 10.1049/ip-nbt:20050045

J P Wikswo, A Prokop, F Baudenbacher, D Cliffel, B Csukas, M Velkovsky

{"title":"Engineering challenges of BioNEMS: the integration of microfluidics, micro- and nanodevices, models and external control for systems biology.","authors":"J P Wikswo, A Prokop, F Baudenbacher, D Cliffel, B Csukas, M Velkovsky","doi":"10.1049/ip-nbt:20050045","DOIUrl":"https://doi.org/10.1049/ip-nbt:20050045","url":null,"abstract":"Systems biology, i.e. quantitative, postgenomic, postproteomic, dynamic, multiscale physiology, addresses in an integrative, quantitative manner the shockwave of genetic and proteomic information using computer models that may eventually have 10(6) dynamic variables with non-linear interactions. Historically, single biological measurements are made over minutes, suggesting the challenge of specifying 10(6) model parameters. Except for fluorescence and micro-electrode recordings, most cellular measurements have inadequate bandwidth to discern the time course of critical intracellular biochemical events. Micro-array expression profiles of thousands of genes cannot determine quantitative dynamic cellular signalling and metabolic variables. Major gaps must be bridged between the computational vision and experimental reality. The analysis of cellular signalling dynamics and control requires, first, micro- and nano-instruments that measure simultaneously multiple extracellular and intracellular variables with sufficient bandwidth; secondly, the ability to open existing internal control and signalling loops; thirdly, external BioMEMS micro-actuators that provide high bandwidth feedback and externally addressable intracellular nano-actuators; and, fourthly, real-time, closed-loop, single-cell control algorithms. The unravelling of the nested and coupled nature of cellular control loops requires simultaneous recording of multiple single-cell signatures. Externally controlled nano-actuators, needed to effect changes in the biochemical, mechanical and electrical environment both outside and inside the cell, will provide a major impetus for nanoscience.","PeriodicalId":87402,"journal":{"name":"IEE proceedings. Nanobiotechnology","volume":"153 4","pages":"81-101"},"PeriodicalIF":0.0,"publicationDate":"2006-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1049/ip-nbt:20050045","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26232926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 55

Freestanding polyelectrolyte multilayers as functional and construction elements. 独立的聚电解质多层作为功能和结构元素。

IEE proceedings. Nanobiotechnology Pub Date : 2006-08-01 DOI: 10.1049/ip-nbt:20050017

M Nolte, A Fery

引用次数: 22

Electrophoretic analysis of gold nanoparticles: size-dependent electrophoretic mobility of nanoparticles. 金纳米颗粒的电泳分析:纳米颗粒的大小依赖的电泳迁移率。

IEE proceedings. Nanobiotechnology Pub Date : 2006-06-01 DOI: 10.1049/ip-nbt:20050043

W Bücking, T Nann

引用次数: 36

Spectral tuning of localised surface plasmon-polariton resonance in metallic nano-crescents. 金属纳米新月形中局部表面等离子体-极化子共振的光谱调谐。

IEE proceedings. Nanobiotechnology Pub Date : 2006-06-01 DOI: 10.1049/ip-nbt:20050016

J Kim, G L Liu, Y Lu, L P Lee

引用次数: 11

Fluorogenic nanocrystals for highly sensitive detection of C-reactive protein. 荧光纳米晶体用于c反应蛋白的高灵敏度检测。

IEE proceedings. Nanobiotechnology Pub Date : 2006-06-01 DOI: 10.1049/ip-nbt:20050048

K-K Sin, C P-Y Chan, W-M Leung, M Seydack, R Renneberg

{"title":"Fluorogenic nanocrystals for highly sensitive detection of C-reactive protein.","authors":"K-K Sin, C P-Y Chan, W-M Leung, M Seydack, R Renneberg","doi":"10.1049/ip-nbt:20050048","DOIUrl":"https://doi.org/10.1049/ip-nbt:20050048","url":null,"abstract":"A solid-phase sandwich fluorescence immunoassay using nanocrystals of a fluorogenic precursor, fluorescein diacetate (FDA), conjugated with monoclonal antibodies for the detection of C-reactive protein (CRP), is described. FDA nanocrystals were coated with distearoylglycerophosphoethanolamine (DSPE), modified with amino(poly(ethylene glycol))(PEG(2000)-Amine) as an interface for coupling biomolecules. CRP was chosen as a model analyte because of its widely accepted role as a marker for acute inflammation and prospective heart failure. A low limit of detection (1.10 microg l(-1)) and high precision (CV = 2.72-9.48%) were achieved. Following the immunoreaction, the monoclonal anti-CRP conjugated nanocrystals were released by hydrolysis and dissolution instigated by the addition of a large volume of organic solvent-sodium hydroxide mixture. Using human serum samples from 66 patients with high heart attack risk and 19 healthy blood donors, this CRP fluorescence immunoassay showed a good correlation to the commercially available, turbidimetric immunoassay for CRP. This result was corroborated by the Bland-Altman plot that showed a mean difference between the two methods of only 0.36+/-1.46 mg l(-1). The study demonstrates that the organic fluorogenic FDA nanocrystals can be applied for the detection of CRP, which is a clinically interesting plasma protein with a low limit of detection.","PeriodicalId":87402,"journal":{"name":"IEE proceedings. Nanobiotechnology","volume":"153 3","pages":"54-8"},"PeriodicalIF":0.0,"publicationDate":"2006-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1049/ip-nbt:20050048","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26108064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Sensors made of RNA: tailored ribozymes for detection of small organic molecules, metals, nucleic acids and proteins. 由RNA制成的传感器:用于检测小有机分子、金属、核酸和蛋白质的定制核糖酶。

IEE proceedings. Nanobiotechnology Pub Date : 2006-04-01 DOI: 10.1049/ip-nbt:20050047

S Müller, D Strohbach, J Wolf

引用次数: 16

Controlled delivery of membrane proteins to artificial lipid bilayers by nystatin-ergosterol modulated vesicle fusion. 通过制氨抑素-麦角甾醇调节的囊泡融合控制膜蛋白向人工脂质双分子层的传递。

IEE proceedings. Nanobiotechnology Pub Date : 2006-04-01 DOI: 10.1049/ip-nbt:20050039

M R R de Planque, M R R de Planque, G P Mendes, M Zagnoni, M E Sandison, K H Fisher, R M Berry, A Watts, H Morgan

{"title":"Controlled delivery of membrane proteins to artificial lipid bilayers by nystatin-ergosterol modulated vesicle fusion.","authors":"M R R de Planque, M R R de Planque, G P Mendes, M Zagnoni, M E Sandison, K H Fisher, R M Berry, A Watts, H Morgan","doi":"10.1049/ip-nbt:20050039","DOIUrl":"https://doi.org/10.1049/ip-nbt:20050039","url":null,"abstract":"The study of ion channels and other membrane proteins and their potential use as biosensors and drug screening targets require their reconstitution in an artificial membrane. These applications would greatly benefit from microfabricated devices in which stable artificial lipid bilayers can be rapidly and reliably formed. However, the amount of protein delivered to the bilayer must be carefully controlled. A vesicle fusion technique is investigated where composite ion channels of the polyene antibiotic nystatin and the sterol ergosterol are employed to render protein-carrying vesicles fusogenic. After fusion with an ergosterol-free artificial bilayer, the nystatin-ergosterol channels do not dissociate immediately and thus cause a transient current signal that marks the vesicle fusion event. Experimental pitfalls of this method were identified, the influence of the nystatin and ergosterol concentration on the fusion rate and the shape of the fusion event marker was explored, and the number of different lipid species was reduced. Under these conditions, the -amyloid peptide could be delivered in a controlled manner to a standard planar bilayer. Additionally, electrical recordings were obtained of vesicles fusing with a planar lipid bilayer in a microfabricated device, demonstrating the suitability of nystatin-ergosterol modulated vesicle fusion for protein delivery within microsystems.","PeriodicalId":87402,"journal":{"name":"IEE proceedings. Nanobiotechnology","volume":"153 2","pages":"21-30"},"PeriodicalIF":0.0,"publicationDate":"2006-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1049/ip-nbt:20050039","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26394433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 29

Nanofunctionalisation for the treatment of peripheral nervous system injuries. 纳米功能化治疗周围神经系统损伤。

IEE proceedings. Nanobiotechnology Pub Date : 2006-04-01 DOI: 10.1049/ip-nbt:20050030

L Pastorino, Federico Caneva Soumetz, C Ruggiero

{"title":"Nanofunctionalisation for the treatment of peripheral nervous system injuries.","authors":"L Pastorino, Federico Caneva Soumetz, C Ruggiero","doi":"10.1049/ip-nbt:20050030","DOIUrl":"https://doi.org/10.1049/ip-nbt:20050030","url":null,"abstract":"A construct based on the electrostatic layer-by-layer self assembly technique has been fabricated, to be used as a tailored device to encourage nerve regeneration. A multilayered nanocoating composed of three precursor bilayers of cationic poly(dimethyldiallylammonium) chloride (PDDA) and anionic poly(styrenesulfonate) (PSS), followed by bilayers of poly-D-lysine (PDL) and antibody specific to transforming growth factor 1 (anti-TGF-1), has been deposited on HYAFF 11. The assembly process has been monitored by quartz crystal microbalance (QCM) for its characterisation and then it has been used on HYAFF 11. Structural studies of the resulting multilayers confirmed stepwise deposition of anti-TGF-1, with an average layer thickness of 2.2+/-0.2 nm and an average surface density of 0.36+/-0.03 mug cm(-2). Scanning electron microscopy has been used to characterise multilayer uniformity. Finally, the immunological activity of the multilayered structure has been assessed. The results show that anti-TGF-1 can be included in its active form in a predetermined multilayered structure onto HYAFF 11 with quantitative control of layer thickness and weight, providing a high tool with great potential in tissue engineering.","PeriodicalId":87402,"journal":{"name":"IEE proceedings. Nanobiotechnology","volume":"153 2","pages":"16-20"},"PeriodicalIF":0.0,"publicationDate":"2006-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1049/ip-nbt:20050030","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26394432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

IEE proceedings. Nanobiotechnology Pub Date : 2006-02-01 DOI: 10.1049/ip-nbt:20050021

Y Yin, H-Y Yeh, J Yin

引用次数: 0

Nanofluidic channels fabrication and manipulation of DNA molecules. 纳米流体通道的制造和DNA分子的操作。

IEE proceedings. Nanobiotechnology Pub Date : 2006-02-01 DOI: 10.1049/ip-nbt:20050044

K Wang, S Yue, L Wang, A Jin, C Gu, P Wang, H Wang, X Xu, Y Wang, H Niu

引用次数: 17