K Wang, S Yue, L Wang, A Jin, C Gu, P Wang, H Wang, X Xu, Y Wang, H Niu
{"title":"纳米流体通道的制造和DNA分子的操作。","authors":"K Wang, S Yue, L Wang, A Jin, C Gu, P Wang, H Wang, X Xu, Y Wang, H Niu","doi":"10.1049/ip-nbt:20050044","DOIUrl":null,"url":null,"abstract":"<p><p>Nanofluidic channel arrays, which have a width of about 40 nm, depth of 60 nm and length of 50 mum, were created using a focused-ion-beam milling instrument on a silicon nitride film swiftly and exactly, as is necessary. Stained -DNA molecules were put inside these sub-100 nm conduits by capillary force and they were stretched and transferred along these conduits, which were dealt with activating reagent Brij aqueous solution in advance. The movements of DNA molecules in these channels were discussed. These nano-structure channels may be useful in the study and analysis of the statics as well as the dynamics of single biomolecules.</p>","PeriodicalId":87402,"journal":{"name":"IEE proceedings. Nanobiotechnology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2006-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1049/ip-nbt:20050044","citationCount":"17","resultStr":"{\"title\":\"Nanofluidic channels fabrication and manipulation of DNA molecules.\",\"authors\":\"K Wang, S Yue, L Wang, A Jin, C Gu, P Wang, H Wang, X Xu, Y Wang, H Niu\",\"doi\":\"10.1049/ip-nbt:20050044\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Nanofluidic channel arrays, which have a width of about 40 nm, depth of 60 nm and length of 50 mum, were created using a focused-ion-beam milling instrument on a silicon nitride film swiftly and exactly, as is necessary. Stained -DNA molecules were put inside these sub-100 nm conduits by capillary force and they were stretched and transferred along these conduits, which were dealt with activating reagent Brij aqueous solution in advance. The movements of DNA molecules in these channels were discussed. These nano-structure channels may be useful in the study and analysis of the statics as well as the dynamics of single biomolecules.</p>\",\"PeriodicalId\":87402,\"journal\":{\"name\":\"IEE proceedings. Nanobiotechnology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2006-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1049/ip-nbt:20050044\",\"citationCount\":\"17\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"IEE proceedings. Nanobiotechnology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1049/ip-nbt:20050044\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"IEE proceedings. Nanobiotechnology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1049/ip-nbt:20050044","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Nanofluidic channels fabrication and manipulation of DNA molecules.
Nanofluidic channel arrays, which have a width of about 40 nm, depth of 60 nm and length of 50 mum, were created using a focused-ion-beam milling instrument on a silicon nitride film swiftly and exactly, as is necessary. Stained -DNA molecules were put inside these sub-100 nm conduits by capillary force and they were stretched and transferred along these conduits, which were dealt with activating reagent Brij aqueous solution in advance. The movements of DNA molecules in these channels were discussed. These nano-structure channels may be useful in the study and analysis of the statics as well as the dynamics of single biomolecules.