Clinical molecular pathology最新文献_第5页

A rapid and efficient method for DNA extraction from paraffin wax embedded tissue for PCR amplification. 一种快速有效的从石蜡包埋组织中提取DNA进行PCR扩增的方法。

Clinical molecular pathology Pub Date : 1996-06-01 DOI: 10.1136/mp.49.3.m179

K Morgan, L Lam, N Kalsheker

引用次数: 8

We're on the Web! 我们在网络上!

Clinical molecular pathology Pub Date : 1996-06-01 DOI: 10.1136/mp.49.3.m125

Jc, Db

引用次数: 0

Differential expression of CD44 variants among meningioma subtypes. CD44变异在脑膜瘤亚型中的差异表达。

Clinical molecular pathology Pub Date : 1996-06-01 DOI: 10.1136/mp.49.3.m140

S O Suzuki, T Iwaki, T Kitamoto, M Mizoguchi, M Fukui, J Tateishi

{"title":"Differential expression of CD44 variants among meningioma subtypes.","authors":"S O Suzuki, T Iwaki, T Kitamoto, M Mizoguchi, M Fukui, J Tateishi","doi":"10.1136/mp.49.3.m140","DOIUrl":"https://doi.org/10.1136/mp.49.3.m140","url":null,"abstract":"Aims/background-CD44 is a widely distributed cell surface molecule which has numerous isoforms generated by alternative splicing. The diverse functions related to the CD44 variants (CD44v) have been reported in various physiological and pathological conditions. The pattern of expression of CD44v among meningioma subtypes was investigated to ascertain whether CD44 variants play a role in a variety of biological processes, such as epithelial differentiation and extracranial metastasis.Methods-Twenty three meningiomas were studied immunohistochemically using novel antibodies directed against CD44 isoforms. Six of the 23 samples were analysed by reverse transcription polymerase chain reaction (RT-PCR), followed by Southern blotting with CD44v specific probes.Results-In meningothelial, fibrous and anaplastic meningiomas, a standard form of CD44 was detected by RT-PCR and was homogeneously expressed in tumour cells when studied immunohistochemically. CD44v was not detected in these subtypes. In secretory meningiomas, however, CD44v isoforms were strongly expressed in the cell clusters that produce secretory granules and also accumulated in the granules. The population of tumour cells immunopositive for CD44v was similar to that which stained with antibodies directed against carcinoembryonic antigen, epithelial membrane antigen and ezrin. On RT-PCR with Southern blotting, only the secretory type showed high level expression of CD44v.Conclusions-CD44v in meningiomas is expressed in relation to tumour cell differentiation towards the epithelial type.","PeriodicalId":87395,"journal":{"name":"Clinical molecular pathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1136/mp.49.3.m140","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26017612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Effect of Wnt-1 antisense RNA on the outgrowth of a mammary adenocarcinoma cell line expressing that oncogene. Wnt-1反义RNA对表达该癌基因的乳腺腺癌细胞生长的影响。

Clinical molecular pathology Pub Date : 1996-06-01 DOI: 10.1136/mp.49.3.m166

J Polanec, Z P Pavelic, W L Myers

引用次数: 3

Gastrointestinal hormone mRNA expression in human colonic adenocarcinomas, hepatic metastases and cell lines. 胃肠激素mRNA在人结肠腺癌、肝转移瘤和细胞系中的表达。

Clinical molecular pathology Pub Date : 1996-06-01 DOI: 10.1136/mp.49.3.m159

G Monges, P Biagini, J F Cantaloube, P De Micco, D Parriaux, J F Seitz, J R Delpero, J Hassoun

{"title":"Gastrointestinal hormone mRNA expression in human colonic adenocarcinomas, hepatic metastases and cell lines.","authors":"G Monges, P Biagini, J F Cantaloube, P De Micco, D Parriaux, J F Seitz, J R Delpero, J Hassoun","doi":"10.1136/mp.49.3.m159","DOIUrl":"https://doi.org/10.1136/mp.49.3.m159","url":null,"abstract":"Aims-(1) To investigate the expression of the four main hormones of the digestive tract by performing reverse transcription polymerase chain reaction (RT-PCR) on a series of samples, comprising tumoral and healthy colonic tissues, hepatic metastases and colonic cell line samples; and (2) to study the patterns of labelling obtained with serological and morphological markers.Methods-After extraction and reverse transcription, gastrin, somatostatin, cholecystokinin (CCK) and transforming growth factor alpha (TGFalpha) mRNAs were detected by PCR and nested PCR using specific primers. The corresponding proteins were detected by immunohistochemistry.Results-The cell lines expressed all four mRNAs. Gastrin mRNA was present in most tumoral and metastatic samples, while the somatostatin transcript was detected in all samples and was frequently overexpressed in the normal colon. TGFalpha mRNA was expressed systematically in tumours of the right and transverse colon, but not in those located in the left colon; the expression of CCK mRNA was systematically absent in the left colon.Conclusions-The data presented here shed some light on the transcriptional events involved in the production of the various hormones present in the gastrointestinal tract, in both healthy and tumoral tissues. The various mRNAs expressed in cell lines are therefore not systematically expressed in the human pathology.","PeriodicalId":87395,"journal":{"name":"Clinical molecular pathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1136/mp.49.3.m159","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26018527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of p53 in leukoplakia and squamous cell carcinoma of the oral mucosa: correlation with expression of Ki67. p53在口腔黏膜白斑和鳞状细胞癌中的表达:与Ki67的表达相关。

Clinical molecular pathology Pub Date : 1996-06-01 DOI: 10.1136/mp.49.3.m170

S Kannan, G J Chandran, K R Pillai, B Mathew, K Sujathan, K R Nalinakumary, M K Nair

{"title":"Expression of p53 in leukoplakia and squamous cell carcinoma of the oral mucosa: correlation with expression of Ki67.","authors":"S Kannan, G J Chandran, K R Pillai, B Mathew, K Sujathan, K R Nalinakumary, M K Nair","doi":"10.1136/mp.49.3.m170","DOIUrl":"https://doi.org/10.1136/mp.49.3.m170","url":null,"abstract":"Aim-To study p53 expression in relation to proliferative status in normal and nondysplastic, dysplastic and malignant lesions of the oral mucosa.Method-The standard avidin-biotin complex (ABC) immunohistochemical staining method was used to study the expression of p53 and Ki67 on frozen sections of oral leukoplakias and carcinomas.Results-Of the leukoplakia and carcinoma samples, 70% expressed p53 in over 5% of cells. In normal mucosa less than 5% of cells expressed p53. The proliferation index, as assessed by expression of Ki67, was highest in the malignant lesions (43%) and lowest in normal mucosa (11%). Statistical analysis revealed that expression of both p53 and Ki67 was correlated significantly with the histopathological stage of the tumour. However, expression of p53 was not correlated with that of Ki67. In leukoplakia lesions with proliferative features p53 immunostaining was less intense than in non-proliferative lesions; this difference was statistically significant.Conclusions-These results emphasise the potential of Ki67 and p53 as biomarkers of carcinogenesis in oral cancer and may also serve as intermediate points for cancer prevention programmes, such as the oral chemopreventive trials. Factors other than p53 may have a more important role in the deregulation of proliferation in pre-malignant oral lesions.","PeriodicalId":87395,"journal":{"name":"Clinical molecular pathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1136/mp.49.3.m170","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26018529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 43

Cellular and molecular pathogenesis 细胞和分子发病机制

Clinical molecular pathology Pub Date : 1996-06-01 DOI: 10.1136/MP.49.3.M183-B

D. Buttle

引用次数: 0

Pathways for Catalysis 催化途径

Clinical molecular pathology Pub Date : 1996-06-01 DOI: 10.1136/mp.49.3.M183-d

R. Herberman

引用次数: 0

Molecular Basis of Oncology 肿瘤学分子基础

Clinical molecular pathology Pub Date : 1996-06-01 DOI: 10.1136/MP.49.3.M183-A

N. R. Lemione