Clinical molecular pathology最新文献_第4页

AgNOR quantity in needle biopsy specimens of prostatic adenocarcinomas: correlation with proliferation state, Gleason score, clinical stage, and DNA content. 前列腺腺癌穿刺活检标本中AgNOR含量与增殖状态、Gleason评分、临床分期及DNA含量的相关性

Clinical molecular pathology Pub Date : 1996-08-01 DOI: 10.1136/mp.49.4.m209

D Trerè, A Zilbering, D Dittus, P Kim, P C Ginsberg, I Daskal

{"title":"AgNOR quantity in needle biopsy specimens of prostatic adenocarcinomas: correlation with proliferation state, Gleason score, clinical stage, and DNA content.","authors":"D Trerè, A Zilbering, D Dittus, P Kim, P C Ginsberg, I Daskal","doi":"10.1136/mp.49.4.m209","DOIUrl":"https://doi.org/10.1136/mp.49.4.m209","url":null,"abstract":"Aims-To define the relation between the quantity of silver stained nucleolar organiser regions (AgNORs) and histological grade, clinical stage, DNA content, and MIB-1 immunostaining in needle biopsy specimens of prostatic adenocarcinomas.Methods-Histological grade was determined according to the Gleason system. AgNOR quantity, DNA content and MIB-1 immunostaining were evaluated by image cytometry on routine histological sections stained with silver, Feulgen reaction and MIB-1 antibody, respectively.Results-The mean AgNOR area increased with increasing Gleason score. A significant difference was found in the AgNOR values between low, intermediate and high grade tumours. Patients with clinically localised tumour (stages A and B) had lower AgNOR values than patients with advanced disease (stages C and D), but the difference in the mean AgNOR values between the two groups was not statistically significant. Non-diploid tumours had a significantly higher mean (SD) AgNOR area than diploid tumours (3.68 (1.04) mum(2)v 2.73 (0.60) mum(2), respectively), while no significant difference was observed in the mean AgNOR values between aneuploid and tetraploid tumours (3.68 (1.04) mum(2)v 3.70 (1.05) mum(2)). When AgNOR and MIB-1-PI values were compared using linear regression analysis, a highly significant correlation was found.Conclusions-These data demonstrate that AgNOR quantity reflects the proliferative potential of prostatic adenocarcinomas, and is significantly related to histological grade and DNA content. The ease of application on routine sections, maintaining the morphological integrity of the tissue, the ability to evaluate selected histological areas of limited size and objective quantification by image cytometry make the AgNOR method particularly suitable for cell kinetic analysis in prostatic needle biopsy specimens.","PeriodicalId":87395,"journal":{"name":"Clinical molecular pathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1136/mp.49.4.m209","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26016851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Counting of apoptotic cells: a methodological study in invasive breast cancer. 凋亡细胞计数:浸润性乳腺癌的方法学研究。

Clinical molecular pathology Pub Date : 1996-08-01 DOI: 10.1136/mp.49.4.m214

H A van de Schepop, J S de Jong, P J van Diest, J P Baak

{"title":"Counting of apoptotic cells: a methodological study in invasive breast cancer.","authors":"H A van de Schepop, J S de Jong, P J van Diest, J P Baak","doi":"10.1136/mp.49.4.m214","DOIUrl":"https://doi.org/10.1136/mp.49.4.m214","url":null,"abstract":"Aims-To arrive at a reproducible sampling technique for counting apoptotic cells in tissue sections of invasive breast cancer that can serve as a protocol for further clinical studies.Methods-In 4 mum thick haematoxylin and eosin stained tissue sections of 12 breast cancers, apoptotic cells, recognised by strict morphological criteria, were counted in consecutive fields of vision at x1000 magnification in a marked area in the most poorly differentiated region of tumour. These counts were regarded as the gold standard. Subsequently, in a systematic sampling experiment, the number of fields that had to be counted to derive an acceptable coefficient of variation (CV) was determined. To compare counts at different magnifications, all fields of vision were also counted at x630 and x400. The intra- and inter-observer reproducibility was tested by repeated measurements at these magnifications in 10 systematically selected fields of vision.Results-Apoptosis seemed to be a rare event, affecting, on average, about 1% of tumour cells. Noticeable clustering of apoptotic cells was observed. The systematic sampling experiment showed that at x1000 magnification, the CV was improved by counting up to 20 fields. When comparing x400 and x630 magnifications with the x1000 magnification, the correlation coefficients were 0.88 and 0.87, respectively. However, the lower magnifications yielded lower counts. With regard to reproducibility, the intra-observer correlation coefficient was 0.91 at x630 and 0.76 at x400. The inter-observer correlation coefficient was 0.77 at x630 and 0.68 at x400.Conclusions-Apoptotic cells can be counted readily in haematoxylin and eosin stained tissue sections. However, a systematic sampling protocol must be followed and cells should be counted at a relatively high magnification to obtain acceptable reproducibility. The suggested protocol will permit further correlative and prognostic studies and the monitoring of the effects of treatment.","PeriodicalId":87395,"journal":{"name":"Clinical molecular pathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1136/mp.49.4.m214","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26016852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 46

Accumulation of allelic losses on chromosome 10 in human gliomas at recurrence. 人类胶质瘤复发时10号染色体等位基因丢失的积累。

Clinical molecular pathology Pub Date : 1996-08-01 DOI: 10.1136/mp.49.4.m218

K Tokiyoshi, T Yoshimine, M Maruno, A K Muhammad, T Hayakawa

{"title":"Accumulation of allelic losses on chromosome 10 in human gliomas at recurrence.","authors":"K Tokiyoshi, T Yoshimine, M Maruno, A K Muhammad, T Hayakawa","doi":"10.1136/mp.49.4.m218","DOIUrl":"https://doi.org/10.1136/mp.49.4.m218","url":null,"abstract":"Aims-To elucidate the implications of allelic loss on chromosome 10 in the malignant progression of human gliomas.Methods-Eight microsatellite loci (D10S249, D10S191, D10S210, D10S219, D10S246, D10S222, D10S221, and D10S212) were analysed for chromosomal deletions in histologically benign and malignant, including recurrent, gliomas. Of the 16 original tumours studied (two astrocytomas, nine anaplastic astrocytomas and five glioblastomas), the histological diagnosis at recurrence was anaplastic astrocytoma in six cases and glioblastoma in 10. Genomic DNA was extracted from formalin fixed, paraffin wax embedded sections. Samples of original and recurrent tumours were paired and amplified using PCR. Samples of histologically normal brain served as controls.Results-Of the original tumours, all five glioblastomas, five (56%) of nine anaplastic astrocytomas and none of the astrocytomas demonstrated loss of heterozygosity (LOH) on chromosome 10. Additional LOH was detected in the five cases of anaplastic astrocytoma that progressed to glioblastoma at recurrence. Additional LOH was not detected in the two cases of astrocytoma that progressed to anaplastic astrocytoma at recurrence. With the exception of one case, additional LOH was observed in the recurrent glioblastomas.Conclusion-LOH was observed at the loci of two adjacent microsatellite markers, D10S222 and D10S221 (10q23-q25), suggesting that this region on chromosome 10 is closely related to progression from anaplastic astrocytoma to glioblastoma.","PeriodicalId":87395,"journal":{"name":"Clinical molecular pathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1136/mp.49.4.m218","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26016853","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

An assessment of the comparative utility of functional and molecular level analyses in the investigation of patients with thrombophilia. 功能和分子水平分析在血栓患者调查中的比较效用评估。

Clinical molecular pathology Pub Date : 1996-08-01 DOI: 10.1136/mp.49.4.m223

B Clark, C Caine, E N McSweeney, B A McVerry, H C Gooi

{"title":"An assessment of the comparative utility of functional and molecular level analyses in the investigation of patients with thrombophilia.","authors":"B Clark, C Caine, E N McSweeney, B A McVerry, H C Gooi","doi":"10.1136/mp.49.4.m223","DOIUrl":"https://doi.org/10.1136/mp.49.4.m223","url":null,"abstract":"Aim-To determine the relation of the low anticoagulant response phenotype with the Factor V Q506 (Leiden) mutation in a cohort of patients with thrombophilia.Methods-Fifty four patients with either a personal or family history of deep vein thrombosis were investigated both for their anticoagulant response by the activated protein C resistance test (APCR) and their genetic status in respect of the Leiden mutation by means of a PCR-RFLP method.Results-Low APCR ratios do not necessarily predict possession of the Leiden mutation. Conversely, normal ratios do not exclude the mutation. Of 14 individuals with low APCR ratios, the Leiden mutation was absent in five. Of the remainder, three were heterozygous and six homozygous. Of nine heterozygote individuals, only three had low APCR ratios. All patients homozygous for the defect had low APCR ratios.Conclusions-These results lend further weight to the hypothesis that the APC resistant phenotype results from more than one genetic defect and indicate the value of combined functional and molecular investigations in all patients with thrombophilia.","PeriodicalId":87395,"journal":{"name":"Clinical molecular pathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1136/mp.49.4.m223","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26016854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The invasiveness of Entamoeba histolytica - a continuing enigma. 溶组织内阿米巴的侵袭性——一个持续的谜。

Clinical molecular pathology Pub Date : 1996-08-01 DOI: 10.1136/mp.49.4.m192

J P Ackers

引用次数: 5

Lymphocytes, cytokines and adhesion molecules in chronic graft versus host disease. 慢性移植物抗宿主病中的淋巴细胞、细胞因子和粘附分子。

Clinical molecular pathology Pub Date : 1996-08-01 DOI: 10.1136/mp.49.4.m225

S Aractingi, E Gluckman, C Le Goué, L Dubertret, E D Carosella

{"title":"Lymphocytes, cytokines and adhesion molecules in chronic graft versus host disease.","authors":"S Aractingi, E Gluckman, C Le Goué, L Dubertret, E D Carosella","doi":"10.1136/mp.49.4.m225","DOIUrl":"https://doi.org/10.1136/mp.49.4.m225","url":null,"abstract":"Aims-To determine which inflammatory and immune pathways are implicated in the development of chronic graft versus host disease (GvHD) and whether differences between these pathways are responsible for the different presentations of chronic GvHD.Methods-Biopsy specimens of diseased and normal skin were obtained from patients presenting with lichen planus-like and sclerodermatous type chronic GvHD. Expression of epidermal cytokines, adhesion molecules and lymphoid surface markers was analysed by means of immunohistochemistry. Apoptosis was detected using the in situ nick endlabelling method.Results-In both GvHD lesion types, CD8+ cells predominated in the epidermis, whereas CD4+ cells were the most prevalentin the dermis. Apoptotickeratinocytes were found in diseased skin only and Fas antibodies labelled a considerable number of keratinocytes. The epidermis in both types of lesions expressed interleukin (IL) 1alpha, tumour necrosis factor (TNF) alpha and intercellular adhesion molecule (ICAM)-1, but dermal vascular cell adhesion molecule (VCAM)-1 expression was restricted to specimens of lichen planus-like GvHD. IL1alpha and E-selectin were expressed in normal looking skin of 55% and 80%, respectively, of patients with lichen planus-like GvHD.Conclusion-The similarity between expression of epidermal cytokines and adhesion molecules (with the exception of VCAM-1) and lymphocyte phenotype in lichen planus-like and sclerodermatous GvHD strongly suggests that the latter occurs as a consequence of the healing process. VCAM-1 distinguishes between lichen planus-like and sclerodermatous lesions. IL1alpha and E-selectin are potential early markers of chronic GvHD.","PeriodicalId":87395,"journal":{"name":"Clinical molecular pathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1136/mp.49.4.m225","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26016855","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Transacting Functions of Human Retroviruses 人类逆转录病毒的交易功能

Clinical molecular pathology Pub Date : 1996-06-01 DOI: 10.1136/MP.49.3.M183-C

D. Pillay

引用次数: 0

Distribution of CD44 messenger RNA in archival paraffin wax embedded tumours and normal tissues viewed by in situ hybridisation. CD44信使RNA在石蜡包埋肿瘤和正常组织中的原位杂交研究。

Clinical molecular pathology Pub Date : 1996-06-01 DOI: 10.1136/mp.49.3.m147

H Gorham, T Sugino, J Bolodeoku, K Yoshida, S Goodison, D Tarin

{"title":"Distribution of CD44 messenger RNA in archival paraffin wax embedded tumours and normal tissues viewed by in situ hybridisation.","authors":"H Gorham, T Sugino, J Bolodeoku, K Yoshida, S Goodison, D Tarin","doi":"10.1136/mp.49.3.m147","DOIUrl":"https://doi.org/10.1136/mp.49.3.m147","url":null,"abstract":"Aims-We have previously demonstrated the abnormal localisation of expression of the CD44 gene in carcinoma cells in cryostat sections of fresh frozen tumour tissues, using radioactive in situ hybridisation (RISH). In order to facilitate further analysis of the expression of this gene in a wider range of neoplastic and non-neoplastic conditions, we have developed a technique which can visualise its low copy number transcripts in archival paraffin wax embedded specimens.Methods-(35)S labelled riboprobes complementary to transcripts from the standard (CD44s) and variant (CD44v) regions of the gene were used on paraffin wax embedded sections of tumours and corresponding normal tissues of the colon, breast and uterine cervix.Results-Elevated levels of signals for CD44s and CD44v transcripts were observed in carcinoma cells relative to their non-neoplastic counterparts in all tissues examined.Conclusion-This method permits easy access to material which can be selected for suitability, handled at room temperature without degradation and relied upon to show good histological detail. Comparison of the results with those on frozen tissues showed similar distributions of signals. Furthermore, the resolution and morphological detail was improved in paraffin wax sections.","PeriodicalId":87395,"journal":{"name":"Clinical molecular pathology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1996-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1136/mp.49.3.m147","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26018525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Nested PCR-SSCP assay for the detection of p53 mutations in paraffin wax embedded bone tumours: improvement of sensitivity and fidelity. 巢式PCR-SSCP法检测石蜡包埋骨肿瘤中p53突变:提高敏感性和保真度。

Clinical molecular pathology Pub Date : 1996-06-01 DOI: 10.1136/mp.49.3.m176

L T Wang, A Smith, B Iacopetta, D J Wood, J M Papadimitriou, M H Zheng

引用次数: 14

Gel Electrophoresis: Proteins 凝胶电泳:蛋白质

Clinical molecular pathology Pub Date : 1996-06-01 DOI: 10.1136/mp.49.3.M182-c

S. Afford

引用次数: 0