{"title":"Effect of citric acid at different pH on the survival of Escherichia coli","authors":"A. Mahé, B. Sabiu, A. Adam, U.Z. Abdullahi,","doi":"10.4314/bajopas.v14i1.11","DOIUrl":"https://doi.org/10.4314/bajopas.v14i1.11","url":null,"abstract":"Microorganisms are included among the major spoilers of food, they achieve this by using the nutrients present in food material. Susceptibility of microorganisms to the most currently used preservatives has been decreasing. Organic acids have been considered as valuable food preservatives. This study aimed to isolate, identify and determine the effect of citric acid at different pH levels on the survival of E. coli. The E. coli was isolated and a pure culture was obtained after series of sub-ulturing on Eosine Methylene Blue agar. The biochemical tests known as IMViC were performed to confirm the presence of the organism. The organism was also identified using polymerase chain reaction (PCR) in which the DNA was extracted, amplified and viewed by gel electrophoresis. The organism was then inoculated in nutrient broth containing citric acid at pH levels of 3.0, 4.5 and 6.0 in different test tubes. Negative controls were included. Results were analyzed using one way ANOVA to compare the means obtained. Results obtained was positive for indole and methyl red tests but negative for VogesProskauer and citrate tests which confirmed the organism. After 24 hours of inoculation, the results of spectrophotometry showed that at pH level of 3.0, the absorbance was lower than the results obtained at pH level of 4.5 and 6.0. This is an indication of higher reduction in the count of the organism at pH of 3.0. There was significant difference between the control and the test groups (p<0.05) but the difference obtained between the test groups were not significant (p>0.05). Results from this study showed that citric acid could not eliminate the whole organism but was effective in inhibiting the growth of the organism dependent on pH level. This indicates that a pH dependent citric acid can be used as a good preservative.","PeriodicalId":8734,"journal":{"name":"Bayero Journal of Pure and Applied Sciences","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77129994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Namadina, A. Mukhtar, S.I. Karaye, F. M. Musa, I.H. Bah, F.Y. Maitama
{"title":"Phytochemical constituents and antibacterial activities of indigenous chewing stick (Anogeissus leiocarpus) stem","authors":"M. Namadina, A. Mukhtar, S.I. Karaye, F. M. Musa, I.H. Bah, F.Y. Maitama","doi":"10.4314/bajopas.v14i1.12","DOIUrl":"https://doi.org/10.4314/bajopas.v14i1.12","url":null,"abstract":"The stems from Anogeissus leiocarpus are commonly used as chewing sticks in Northern Nigeria. If properly used, the chewing sticks have proven to be effective in removing dental plaque due to mechanical cleaning and enhanced salivation. Chewing sticks from other plants have been shown to display antimicrobial activities against a broad spectrum of microorganisms. However, there is limited information available in Northern Nigeria on the chemical composition, antimicrobial properties and the ability of the plants under study to prevent bacterial adhesion to tooth surface. Therefore, the purpose of this study was to ascertain the phytochemical and antibacterial properties of Anogeissus leiocarpus and correlate the results obtained to their ethnomedicinal uses as chewing sticks. Powdered stem was exhaustively extracted using methanol at room temperature for 72 hours. Antibacterial activities of the methanol extract was assessed using the agar well diffusion methods against the oral pathogens, Staphylococcus aureus, Streptococcus salivarius, Streptococcus pyogenes, Streptococcus mutans and Streptococcus sanguinis. Acute toxicity study was achieved using Lorke method. Phytochemicals which include flavonoid, steroid, triterpenes, alkaloids, tannins, carbohydrate, glycoside, phenols were detected in the extracts while anthraquinones was absent. The antibacterial results revealed that, the methanol extract had promising antibacterial activity. S. aureuswas found to be the most susceptible bacteria at 500mg/ml with inhibition of 22 mm, Streptococcus salivarius, Streptococcus pyogenes and Streptococcus sanguinis were inhibited at 16 mm while Streptococcus mutans showed inhibition of 14 mm. The extract have MIC and MBC of 31.25 mg/ml and 62.5 mg/ml respectively against all the tested clinical isolates. The LD50 of Anogeissus leiocarpus was found to be greater than 5000 mg/kg and could be considered safe for consumption.","PeriodicalId":8734,"journal":{"name":"Bayero Journal of Pure and Applied Sciences","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72622313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Nuhu, H. Haruna, M. Namadina, S. Haruna, M. Abdulrazak, B.D. Yakasai, M. Adamu, F.Y. Maitama, S.I. Karaye, A. Muttaka
{"title":"Pharmacognostic, antioxidant and acute toxicity studies of Vitellaria paradoxa stem bark and root extracts","authors":"Y. Nuhu, H. Haruna, M. Namadina, S. Haruna, M. Abdulrazak, B.D. Yakasai, M. Adamu, F.Y. Maitama, S.I. Karaye, A. Muttaka","doi":"10.4314/bajopas.v14i1.15","DOIUrl":"https://doi.org/10.4314/bajopas.v14i1.15","url":null,"abstract":"Most of biochemical reactions in the body generates Reactive Oxygen Species (ROS)which are involved in the pathogenesis of oxidative stress-related disorders like diabetes,nephrotoxicity, cancer, cardiovascular disorders, inflammation and neurological isorders. Antioxidants are used to protect the cells or tissues against potential attack by ROS. Most medicinal plants possess a rich source of antioxidants such as flavonoids, phenols, tannins, alkaloids among others. These phytochemicals are currently pursued as an alternative and complimentary drug. The study was carried out to ROS. Most medicinal plants possess a rich source of antioxidants such as flavonoids, phenols, tannins, alkaloids among others. These phytochemicals are currently pursued as an alternative and complimentary drug. The study was carried out to determine Pharmacognostic, antioxidant and acute toxicity of Vitellaria paradoxa root and stem bark. The Vitellaria paradoxa was extracted with water and methanol, screened for their phytochemical properties and antioxidant effects. Chemo-microscopical studies revealed the presence of cellulose cell wall, lignin, calcium oxalate crystals, tannins, starch and mucilage while calcium carbonate was absent in the stem bark but present in the root. The average moisture contents were 7.30% and 6.80% in stem bark and root respectively. The water and ethanol/alcohol extractives were 24.0% and 20.0% for stem while 25.50% and 19.0% for the root respectively. Alkaloid, flavonoids, saponins, tannins, steroids, triterpenes, carbohydrate and phenols were detected in both aqueous and methanol extracts while anthraquinones was absent in all the extracts. The DPPH radical scavenging ability of the extract showed the following trend Ascorbic acid ˃ Stem bark extract˃ Root extract. Toxicity of the samples was expressed as LD50, it was found above 5000 mg/kg and did not cause mortality in all the tested rats. These results suggested that Vitellaria paradoxa root and stem bark have moderate antioxidant potentials. Further study is necessary for isolation and characterization of active antioxidant agents which can be used to treat various oxidative stress related diseases.","PeriodicalId":8734,"journal":{"name":"Bayero Journal of Pure and Applied Sciences","volume":"3 2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81362818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Intracranial compartment volumes and their distribution among clinically diagnosed patients with normal pressure hydrocephalus and brain atrophy in a Nigerian population","authors":"A. Mohammed, C. Anthony","doi":"10.4314/bajopas.v14i1.10","DOIUrl":"https://doi.org/10.4314/bajopas.v14i1.10","url":null,"abstract":"Normal pressure hydrocephalus is a form of non-obstructive hydrocephalus characterized by the triad of symptoms. These symptoms are typical or atypical. Because of its potential reversibility, researchers have paid attention to its diagnosis and in differentiating it with other forms of dementia such as brain atrophy. To determine the intracranial compartment volumes and their differences among patients with NPH and Brain Atrophy (BA). This was a cross-sectional study involving consenting patients diagnosed with NPH and BA who were referred for routine brain CT. Medical conditions known to influence intracranial volume were excluded. Age -matched normal control were clinically and radiologically confirmednormal and were also recruited. Intracranial volumes and CSF distribution determination was based on Cavalieri test point computation principle. Test point summation was on a locally developed software for this purpose. Overall, the values of BA recorded higher mean values for most of the intracranial compartment volumes. Intraventricular volume was consistently higher in NPH groups. Statistical difference exists among intraventricular and total intracranial CSF volume across patients with BA, NPH and control. A post hoc test revealed control-NPH comparison across these variables. Control-BA comparison was evident in total intracranial CSF volume only. Higher mean values of intracranialcompartment volumes were observed in patients with BA than among patients with NPH and their control. Anthropometric indices did not show any difference between patients with BA, NPH and their control. These indices can be used as basis in objectively differentiating NPH from BA.","PeriodicalId":8734,"journal":{"name":"Bayero Journal of Pure and Applied Sciences","volume":"59 3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75989613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Radio frequency identification (RFID) and database- based attendance management system","authors":"U.S. Ahmad, A. A. Bisu, F. A. Umar, U. Balarabe","doi":"10.4314/bajopas.v14i1.16","DOIUrl":"https://doi.org/10.4314/bajopas.v14i1.16","url":null,"abstract":"Effective record and management of students and staff attendance of academic and non academic activities/events are vital for the smooth functioning of the educational system. This is still a difficult task in most institutions, particularly in Nigeria with a large number of staff and students attending different academic and non-academic functions. This is even more difficult to manage when the traditional method of paper and pen is used to record attendance, prone to errors, and in most cases lack integrity due to manual handling of the record. Electronics and Information Communications Technologies (ICT) can be deployed to help mitigate these problems and improve reliability, ease, speed, efficiency, effectiveness, and integrity of recording and managing attendance. In this work, we used Radio Frequency Identification (RFID) and database management to provide an alternative solution that addresses issues of objects (humans and non-humans) authentication, authorization, and record management with high accuracy, reliability, and integrity using RFID-Arduino technology. The system works by reading staff’s and/or student’s details stored in a unique RF tag wirelessly through theRF reader and then matched and stored the record in the system’s database. Attendancemarking was achieved by matching the scanned ID with the database record. The system was successfully implemented and tested with 10 students and staff participants with the feature of exporting the records into excel format for statistical analysis and performance evaluation.","PeriodicalId":8734,"journal":{"name":"Bayero Journal of Pure and Applied Sciences","volume":"64 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79568459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The micronutrient status of some soil supporting oil palm plantationin Nifor, Benin City, Benin","authors":"J. S. Ogeh, A. Imogie, C. Maduakor","doi":"10.4314/bajopas.v14i1.13","DOIUrl":"https://doi.org/10.4314/bajopas.v14i1.13","url":null,"abstract":"This study was conducted to assess the micronutrient status of soils under three different management practices at the Nigerian Institute for Oil Palm Research (NIFOR), Benin City, Edo State, Nigeria. Soil samples were collected from three different fields Research (NIFOR), Benin City, Edo with different management practices. The samples were air dried and analyzed for Fe, Zn, Cu, and Mn contents using standard method. Data was subjected to ANOVA and correlation studies. Result showed that soil micronutrient status was generally low as revealed by their estimated levels of availability: field 53 (Mn = 0.0333; Zn = 3.723; Cu = 2.04; Fe = 6.4), field 56 (Mn = 0.0367; Zn = 3.737; Cu = 1.12; Fe = 7.0939), field 71 (Mn = 0.0167; Zn = 3.860; Cu = 2.89; Fe = 7.243). Fe and Zn were above critical level for the 3 fields. Mn was below the critical level for the 3 fields and was significantly higher in field 56 (Orlu and Kulfo series) (p≤ 0.05). They were all similar. Fe was significantly different in field 53 (Kulfo and Alagba series) but similar in fields 56 and 71 (p≤ 0.05). Cu was significantly different in the 3 fields tested with field 71 (Orlu and Alagba series) higher than the critical level. Correlation studies showed that pH had a positive and significant correlation with Fe and Zn as observed in fields 71 and 56 (r = 0.9934, P≤ 0.05) and (r = 0.9997, P ≤ 0.05) respectively. Cu was the only micronutrient that has correlation with clay on each of the fields: 53, 56 and 71 (r = 0.9929, P≤0.05) (r = 0.9988, P≤ 0.05) and (r = 0.9967, P ≤ 0.05) respectively. Strong correlation existed between clay and Fe in field 53 (r = 1, P≤ 0.05). The concentration of the micronutrients (Fe, Mn,Zn Cu), in field 53 were not extreme (neither the highest nor lowest) when comparing the three fields indicating that BMP (Best Management Practice) may be the best for maintaining good micronutrient level in soils supporting oil palm. Fields 53 and 56 is adequate in Fe and Zn but not adequate in Mn and Cu while field 71 was adequate in Zn, Fe, and Cu but not in Mn. Based on the result of this experiment, adequate fertilizers of these micronutrients as their respective sulphates or impurities should be added to maintain proper micronutrient level. ","PeriodicalId":8734,"journal":{"name":"Bayero Journal of Pure and Applied Sciences","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79051946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Assessment of health risks from consumption of food crops fumigated with metal based pesticides in Gwadam, Gombe State, Nigeria","authors":"U. Bawa, A. Ahmad, J.N. Ahmad, A. Ezra","doi":"10.4314/bajopas.v14i1.14","DOIUrl":"https://doi.org/10.4314/bajopas.v14i1.14","url":null,"abstract":"Intensive use of agrochemicals has led to build of heavy metals in the soil ecosystem and their transfer to edible parts of crops. This study was aimed to determine the heavy metals (Cd, Pb, Cr, Cu and Zn) concentrations in some twenty commonly used pesticides in northern Nigeria, and health risk associated from the consumption of food crops fumigated with these pesticides as the only source of metal contamination. Heavy metals content in pesticides, food crops and soils were analyzed after acid digestion using atomic absorption spectrometry. Traces of heavy metals (Cd, Pb, Cr, Cu and Zn) were detected in most of the pesticides. The concentrations of heavy metals in crops fumigated with pesticides ranged from 0.33-4.68, 1.75-38.08, 0.67-16.83, 9.01-436.75, 0.17-20.80mg/kg for Cd, Pb, Cr, Cu, and Zn respectively. The mean concentrations of Cd, Pb, and Cr in all the crops were above WHO, (2019) permissible limit. Heavy metals in soils of corresponding crops were below the permissible limits by UNEP, (2013) and NESREA, (2011). Bioaccumulation factor BAF showed high BAF>1 for Cu and Pb in all the studied crops,while pollution index value revealed contamination for Cd and Pb in all the studied crops. Hazard quotient showed potential health risk from the consumption of only Capsicumannuum for Pb. However, consumers may experience advance health risk through the consumption of Oryza sativa, Zea mays, Solanumlycopersicum, Capsicumfrustescens for all metals (Hazard index). Hence, there is the need for screening of heavy metals in pesticides and monitoring of metals contents in food crops.","PeriodicalId":8734,"journal":{"name":"Bayero Journal of Pure and Applied Sciences","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88432838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Experimental assessment on preparation of biodegradable polythylene/polylatic acid-chithosan composite from renewable resources","authors":"B. U. Gumel, S. M. Gumel, A. Bawa, A. Auwal","doi":"10.4314/bajopas.v14i1.7","DOIUrl":"https://doi.org/10.4314/bajopas.v14i1.7","url":null,"abstract":"Due to poor degradability and contamination risk of synthetic plastics, utilization of renewable resources is encouraged. Biobased thermoplastic polymers from renewable resource that is inexpensive, biodegradable, compostable and renewably non-toxic, is focused. In this paper mixtures of synthetic and natural polymers were used as a potential option to reduce pollution by plastic waste. The study is aimed at assessing utilization of sweet potato waste as a source of bioplastic for package application, the polymer was modified with a biopolymer chitosan to obtain polylactic acid-chitosan plastic. The developed polymer matrix was blended with polyethylene to obtain biodegradable packaging material. The bioplastic was characterized using Fourier Transformed Infra-Red Spectroscopy (FTIR) and scaning electron microscope (SEM). Physical and mechanical properties of the composites were evaluated by measuring enzymatic degradation, tensile strength, and elongation at break. The results have shown that the film obtained have homogeneous surface by (SEM). Mechanical properties of the bio plastics revealed that tensile strength increases with increases in the concentration of chitosan and hence, the elongation at break decreases with increase in chitosan content. While the fastest enzymatic degradation was observed to have high microbial growth on the bio plastics with high content of Chitosan-Polylactic acid.","PeriodicalId":8734,"journal":{"name":"Bayero Journal of Pure and Applied Sciences","volume":"79 2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78143273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Solid state synthesis, characterization and anti- microbial studies of some transition metal complexes of a schiff base ligand derived from parabenzoquinone and glycine","authors":"M. Kurawa, J. Jaafar","doi":"10.4314/bajopas.v14i1.9","DOIUrl":"https://doi.org/10.4314/bajopas.v14i1.9","url":null,"abstract":"Schiff base was synthesized by mixing parabenzoquinone and glycine mechanochemically. Its corresponding complexes of Cu, Co and Mn were synthesized by grinding metal (II) carbonates. The synthesized compound were characterized by elemental analysis, infrared spectroscopy, melting/decomposition temperature determination and conductivity measurements .The infrared spectroscopic analysis of the ligand shows a peak at 1620cm-1 confirm the presence of azomethane while the shift to 1602 cm-1,1623.15cm-1, 1635.69cm-1 in Mn, Cu, Co complexes respectively confirmed bond formation from azo-group to metal. The melting point temperature of the ligand at 128.20oC show that it is stable at low temperature while the decomposition temperature of Mn, Cu, Co complexes at 1900C,188.40C,2050C shows that they are stable conductivity measurement shows that the complexes were found to be non-electrolytic as compared with the theoretical values. The ligand and its respective complexes showed antimicrobial activities against some bacteria and fungi at high concentrations(1000 μg/disc and 500μg/disc) respectively while some showed no activity at all concentrations. However, Co complex shows significant activity against all the species at low concentration (250μ/disc).","PeriodicalId":8734,"journal":{"name":"Bayero Journal of Pure and Applied Sciences","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81052678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Short communication extraction and phytochemical analysis of Hyptis spicigera leaves","authors":"A. Idi, I. Muhammad","doi":"10.4314/bajopas.v14i1.3","DOIUrl":"https://doi.org/10.4314/bajopas.v14i1.3","url":null,"abstract":"Plants are recognized in the pharmaceutical industry for their broad structural diversity as well as their wide range of pharmacological activities, which is due to their biologically active compounds known as phytochemicals. The present study reports the extraction, thin layer chromatography and screening of phytochemical constituent of Hyptis spicigera leaves. The thin layer chromatography of the leave extract shows 11 bands with Rf values of 0.03, 0.06, 0.09, 0.12, 0.17, 0.19, 0.20, 0.23 and 0.31 respectively. Qualitative phytochemical screening showed the presence of alkaloids, flavonoids, steroids, emodins, and cardiac glycoside while phenols, tannins, terpenoids, tri terpenoids and anthraquinones were absent. The presences of these phytochemicals showed that Hyptis spicigera leaves may be useful for medicinal purpose.","PeriodicalId":8734,"journal":{"name":"Bayero Journal of Pure and Applied Sciences","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74754414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}