Postepy higieny i medycyny doswiadczalnej (Online)最新文献_第2页

GPR120: Mechanism of action, role and potential for medical applications. GPR120:作用机制、作用和医疗应用潜力。

Postepy higieny i medycyny doswiadczalnej (Online) Pub Date : 2017-11-19 DOI: 10.5604/01.3001.0010.5809

Hanna Karakuła-Juchnowicz, Joanna Róg, Dariusz Juchnowicz, Justyna Morylowska-Topolska

{"title":"GPR120: Mechanism of action, role and potential for medical applications.","authors":"Hanna Karakuła-Juchnowicz, Joanna Róg, Dariusz Juchnowicz, Justyna Morylowska-Topolska","doi":"10.5604/01.3001.0010.5809","DOIUrl":"https://doi.org/10.5604/01.3001.0010.5809","url":null,"abstract":"G protein-coupled receptors (GPCRs) constitute a family of transmembrane proteins that mediate many cellular processes. GPR120/FFAR4, a receptor from this family that is activated by fatty acids, has received considerable attention recently. This paper presents a literature review concerning the role of GPR120 and its mechanism of action in animal and human studies as well as the potential use of GPR120 for the treatment of chronic diseases. Two electronic databases - Medline and Google Scholar - were searched for available studies addressing the review topic that were written in English and published from 2000 to June 2017. The following key terms were used in the search: GPR120, FFA4, GPR120 agonist, PUFAs, EPA, DHA, adipocyte, obesity, hyperlipidemia, inflammation, cancer, diabetes, insulin resistance, taste, atherogenesis, hepatis, central nervous system. In humans, GPR120 expression is expressed in macrophages, eosinophils, and adipose tissue, in cells of the tongue, liver, lungs, small and large intestine, gastric mucosa, and pancreas, in the central nervous system and placental microvilli. Medium- and long-chain fatty acids act as ligands for the receptor. Through the internalization of beta-arrestin-2 complex and the inhibition of NF-κB, GPR120 mediates the activation of the cell's anti-inflammatory mechanisms. The receptor is also involved in the maturation of adipocytes, the modulation of insulin signalling pathways, the regulation of glucose metabolism, and the secretion of intestinal hormones. GPR120 is a promising target for the treatment of numerous diseases, whose pathophysiology is associated with low-grade inflammation. As a result of intensive searches, a likely group of synthetic agonists of the receptor was determined with potential therapeutic applications in conditions such as obesity, impaired carbohydrate metabolism, inflammatory bowel diseases, cancer, mental disorders.","PeriodicalId":87132,"journal":{"name":"Postepy higieny i medycyny doswiadczalnej (Online)","volume":"71 0","pages":"942-953"},"PeriodicalIF":0.0,"publicationDate":"2017-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35639961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Between biology and medicine: perspectives on the use of dendritic cells in anticancer therapy. 在生物学和医学之间:树突状细胞在抗癌治疗中的应用前景。

Postepy higieny i medycyny doswiadczalnej (Online) Pub Date : 2017-11-19 DOI: 10.5604/01.3001.0010.5808

Agnieszka Szczygieł, Elżbieta Pajtasz-Piasecka

{"title":"Between biology and medicine: perspectives on the use of dendritic cells in anticancer therapy.","authors":"Agnieszka Szczygieł, Elżbieta Pajtasz-Piasecka","doi":"10.5604/01.3001.0010.5808","DOIUrl":"https://doi.org/10.5604/01.3001.0010.5808","url":null,"abstract":"Dendritic cells (DCs), as a link between innate and adaptive immunity, play a pivotal role in maintaining homeostasis of the immune system. The DC population is characterized by heterogeneity; it consists of many subpopulations which, despite their phenotypic and localization differences, play an essential function - they are professional antigen presenting cells. Due to their role, DCs can be utilized in a new cancer treatment strategy. Their main purpose is to generate an anticancer response leading to the elimination of cancer cells. The tumor microenvironment, abundant in immunosuppressive factors (e.g. IL-10, TGF-β, Arg1, IDO), impairs the proper function of DCs. For this reason, various strategies are necessary for ex vivo preparation of DC-based vaccines and for the support of in vivo DCs to fight against tumors. DC-based vaccines are combined with other forms of immunotherapy (e.g. blockade of immune checkpoint molecules, e.g. PD-1 or CTLA-4) or conventional types of therapies (e.g. chemotherapy). Despite the enormous progress that has been made in anticancer therapy in the past two decades, there are still many unresolved issues regarding the effectiveness of the DCs usage. In this paper we described, in both a mouse and a human subject, a series of DC subpopulations, differentiating in normal conditions or under the influence of cancer microenvironment. We listed factors affecting the quality of the in vivo and ex vivo generations of antitumoral responses, significant from a therapeutic point of view. Moreover, the most important strategies for the use of DCs in anticancer therapies, as well as further developments on this field, have been discussed.","PeriodicalId":87132,"journal":{"name":"Postepy higieny i medycyny doswiadczalnej (Online)","volume":"71 0","pages":"921-941"},"PeriodicalIF":0.0,"publicationDate":"2017-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35639963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Evolution of sanitary-epidemiological services in Poland in the years 1944-2014. 1944-2014年波兰卫生流行病学服务的演变。

Postepy higieny i medycyny doswiadczalnej (Online) Pub Date : 2017-11-08 DOI: 10.5604/01.3001.0010.5667

Marek L Grabowski, Bożena Kosińska, Józef P Knap

引用次数: 1

Instruments to assess stigmatization in dermatology. 评估皮肤病学污名化的仪器。

Postepy higieny i medycyny doswiadczalnej (Online) Pub Date : 2017-11-03 DOI: 10.5604/01.3001.0010.5607

Dimitre Dimitrov, Jacek C Szepietowski

{"title":"Instruments to assess stigmatization in dermatology.","authors":"Dimitre Dimitrov, Jacek C Szepietowski","doi":"10.5604/01.3001.0010.5607","DOIUrl":"https://doi.org/10.5604/01.3001.0010.5607","url":null,"abstract":"Stigmatization is the assignment of negative perceptions to an individual because of a perceived difference from the population at large. Skin conditions are frequently the reason of social rejection with a consequent negative influence on the personal and social life of patients. The aim of the current study was to review the available instruments that can be successfully utilized to measure the stigmatization level among dermatological patients. We performed our search on PubMed up to November 2016 and utilized combinations of key phrases containing such words as stigmatization, skin, dermatology, names of various skin conditions (psoriasis, vitiligo, acne, etc.), measurement. The search found a considerable number of articles - 548. After filtering them through a precise selection process, 58 articles remained. We concentrated only on the methodological aspects to assess stigmatization in various dermatoses. The review ascertained that there exist numerous instruments in the form of questionnaires. They were utilized in various researches in order to assess the stigmatization level in patients with skin problems. We divided them into two main groups: dermatology specific instruments (6 questionnaires) and dermatosis/disease specific ones (8 questionnaires). It is recommended to use dermatology-specific instruments to compare the stigmatization level in various skin conditions. They can be utilized as well as a first line tools to study the feeling of stigmatization in specific skin diseases; however, where it is possible, they should be supplemented with the disease-specific instrument for deeper analysis of both qualities of life and stigmatization.","PeriodicalId":87132,"journal":{"name":"Postepy higieny i medycyny doswiadczalnej (Online)","volume":"71 0","pages":"901-905"},"PeriodicalIF":0.0,"publicationDate":"2017-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35564286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Predation among microorganisms: A huge potential of interspecies dependencies. 微生物间的捕食:种间依赖的巨大潜力。

Postepy higieny i medycyny doswiadczalnej (Online) Pub Date : 2017-11-03 DOI: 10.5604/01.3001.0010.5608

Justyna Kowalska, Marcin Włodarczyk

{"title":"Predation among microorganisms: A huge potential of interspecies dependencies.","authors":"Justyna Kowalska, Marcin Włodarczyk","doi":"10.5604/01.3001.0010.5608","DOIUrl":"https://doi.org/10.5604/01.3001.0010.5608","url":null,"abstract":"There are many interactions between species (including bacteria) in the environment. One of them is predation, which always leads to the death of a prey. Described in this review Bdellovibrio bacteriovorus (Deltaproteobacteria) and Micavibrio aeruginosavorus (Alfaproteobacteria) are uniflagellate, rod shaped and curved obligate predators of Gram-negative bacteria. Both species belong to the group of BALOs (Bdellovibrio and like organisms). B. bacteriovorus use periplasmic predatory strategy and M. aeruginosavorus are epibiotic hunters. BALOs have found application in both medicine in combating microorganisms responsible for food poisoning and outside of medicine (agriculture and food) as plant protection products and as measures used to prevent the spoiling of food. As a result of searching for effective therapies in the treatment of infections caused by drug-resistant strains of bacteria, it has been shown that predators feed on pathogenic bacteria without showing immunogenicity to humans. Predatory bacteria are able to destroy the multi - and single-species biofilms. Recent studies have indicated the possibility of B. bacteriovorus to destroy the biofilm formed by Staphylococcus aureus. It is postulated that a double predatory strategy of B. bacteriovorus and harmless BALOs towards mammalian cells could be used to treat infections in vivo, particularly in those cases when standard therapy fails.","PeriodicalId":87132,"journal":{"name":"Postepy higieny i medycyny doswiadczalnej (Online)","volume":"71 0","pages":"906-914"},"PeriodicalIF":0.0,"publicationDate":"2017-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35564287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The oral cavity - potential source of stem cells. 口腔——干细胞的潜在来源。

Postepy higieny i medycyny doswiadczalnej (Online) Pub Date : 2017-10-19 DOI: 10.5604/01.3001.0010.5385

Rafał Brożek, Maciej Kurpisz, Ryszard Koczorowski

{"title":"The oral cavity - potential source of stem cells.","authors":"Rafał Brożek, Maciej Kurpisz, Ryszard Koczorowski","doi":"10.5604/01.3001.0010.5385","DOIUrl":"https://doi.org/10.5604/01.3001.0010.5385","url":null,"abstract":"The purpose of this review is to present the current knowledge regarding the hierarchy of stem cells originating from the oral cavity, which could have a potential value when applied to regenerative stomatology. It must be particularly emphasized that the heterogenous nature of its biology and function within oral compartment may predispose them to different types of applications. Stem cells can be perceived as immature, primitive and unspecialized types of cells with the ability to proliferate, self-renew and differentiate into specialized progeny according to the compartmental signaling. Their presence in tissue reservoirs was already discovered in many organs and tissues as well as in the stomatognathic system. The oral cavity appears to be an exceptionally attractive site to acquire stem cells. The common presence and easy access to these cells in dental and peridental tissues provides a real chance to apply them for therapeutic purposes. Such an opportunity would also be neutral to bioethical and moral issues, assuming autologous stem cells employment. Many authors suspect that stem cells have epigenetic memory, so some of their features can be inherited through generations. They are not connected, however, with DNA sequence modifications. It is, therefore, justified to apply the cells, which have the oral cavity as their natural reservoir, in interventions associated with tissue engineering within the stomatognathic system. An increasing number of clinical trials, among which the number of randomized studies with large group of patients is progressively carried out, allows for a prediction that shortly therapeutic methods based on stem cells of dental origin may be implemented to the routine repertoire of clinical practice.","PeriodicalId":87132,"journal":{"name":"Postepy higieny i medycyny doswiadczalnej (Online)","volume":"71 0","pages":"881-894"},"PeriodicalIF":0.0,"publicationDate":"2017-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35564288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The mRNA expression levels of uncoupling proteins 1 and 2 in mononuclear cells from patients with metabolic disorders: obesity and type 2 diabetes mellitus. 代谢紊乱:肥胖和2型糖尿病患者单核细胞解偶联蛋白1和2的mRNA表达水平

Postepy higieny i medycyny doswiadczalnej (Online) Pub Date : 2017-10-19 DOI: 10.5604/01.3001.0010.5386

Sona Margaryan, Agata Witkowicz, Anna Partyka, Levon Yepiskoposyan, Gayane Manukyan, Lidia Karabon

{"title":"The mRNA expression levels of uncoupling proteins 1 and 2 in mononuclear cells from patients with metabolic disorders: obesity and type 2 diabetes mellitus.","authors":"Sona Margaryan, Agata Witkowicz, Anna Partyka, Levon Yepiskoposyan, Gayane Manukyan, Lidia Karabon","doi":"10.5604/01.3001.0010.5386","DOIUrl":"https://doi.org/10.5604/01.3001.0010.5386","url":null,"abstract":"Introduction: Type 2 diabetes mellitus (T2DM) and obesity are metabolic disorders whose major hallmark is insulin resistance. Impaired mitochondrial activity, such as reduced ratio of energy production to respiration, has been implicated in the development of insulin resistance. Uncoupling proteins (UCPs) are proton carriers, expressed in the mitochondrial inner membrane, that uncouple oxygen consumption by the respiratory chain from ATP synthesis.Aim: The aim of the study was to determine transcriptional levels of UCP1 and UCP2 in peripheral blood mononuclear cells (PBMCs) from patients with metabolic disorders: T2DM, obesity and from healthy individuals.Material/methods: The mRNA levels of UCP1, UCP2 were determined by Real-Time PCR method using Applied Biosystems assays.Results: The UCP1 mRNA expression level was not detectable in the majority of studied samples, while very low expression was found in PBMCs from 3 obese persons. UCP2 mRNA expression level was detectable in all samples. The median mRNA expression of UCP2 was lower in all patients with metabolic disorders as compared to the controls (0.20+0.14 vs. 0.010+0.009, p=0.05). When compared separately, the differences of medians UCP2 mRNA expression level between the obese individuals and the controls as well as between the T2DM patients and the controls did not reach statistical significance.Conclusions: Decreased UCP2 gene expression in mononuclear cells from obese and diabetic patients might contribute to the immunological abnormalities in these metabolic disorders and suggests its role as a candidate gene in future studies of obesity and diabetes.","PeriodicalId":87132,"journal":{"name":"Postepy higieny i medycyny doswiadczalnej (Online)","volume":"71 0","pages":"895-900"},"PeriodicalIF":0.0,"publicationDate":"2017-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35564289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

The impact of agonists and antagonists of TLR3 and TLR9 on concentrations of IL-6, IL10 and sIL-2R in culture supernatants of peripheral blood mononuclear cells derived from patients with systemic lupus erythematosus. TLR3和TLR9激动剂和拮抗剂对系统性红斑狼疮患者外周血单个核细胞培养上清中IL-6、il - 10和sIL-2R浓度的影响

Postepy higieny i medycyny doswiadczalnej (Online) Pub Date : 2017-10-13 DOI: 10.5604/01.3001.0010.5266

Agnieszka Klonowska-Szymczyk, Liliana Kulczycka-Siennicka, Tadeusz Robak, Piotr Smolewski, Barbara Cebula-Obrzut, Ewa Robak

{"title":"The impact of agonists and antagonists of TLR3 and TLR9 on concentrations of IL-6, IL10 and sIL-2R in culture supernatants of peripheral blood mononuclear cells derived from patients with systemic lupus erythematosus.","authors":"Agnieszka Klonowska-Szymczyk, Liliana Kulczycka-Siennicka, Tadeusz Robak, Piotr Smolewski, Barbara Cebula-Obrzut, Ewa Robak","doi":"10.5604/01.3001.0010.5266","DOIUrl":"https://doi.org/10.5604/01.3001.0010.5266","url":null,"abstract":"Toll-like receptors (TLR), especially TLR3, 7 and 9, play an important role in the pathogenesis of systemic lupus erythematosus (SLE). In our study blood was collected from 16 patients with SLE and from 8 healthy volunteers. Concentrations of IL-6, IL-10 and sIL-2R were measured by ELISA in mononuclear cell culture supernatant after 24 hours of stimulation by agonists and antagonists of TLR3 and 9 (for TLR3-poli I/C, resveratrol and for TLR9-ODN2006, IRS 945). Stimulation of TLR9 by ODN2006 led to an increase of IL-6 concentration in cell culture supernatants from the cells of healthy volunteers compared with unstimulated cells from controls. Inhibition of TLR3 activation by resveratrol caused a significantly lower concentration of IL-10 in cell culture supernatants derived from both patients and healthy donors. Moreover, resveratrol significantly decreased the level of IL-10 and sIL-2R in culture supernatants of cells derived from patients with active disease compared to the inactive stage. A positive correlation was also found between IL-6 concentration following ODN2006 administration and disease activity. In conclusions, our results indicate that TLRs play a role in the modulation of the inflammatory response in SLE patients. This suppressive action on IL-10 synthesis demonstrated by resveratrol suggests that it may be useful in SLE therapy.","PeriodicalId":87132,"journal":{"name":"Postepy higieny i medycyny doswiadczalnej (Online)","volume":"71 0","pages":"867-875"},"PeriodicalIF":0.0,"publicationDate":"2017-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35614529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Ferritin as a potential biomarker of efficacy of treatment of atrial fibrillation - preliminary report. 铁蛋白作为房颤治疗疗效的潜在生物标志物——初步报告。

Postepy higieny i medycyny doswiadczalnej (Online) Pub Date : 2017-10-13 DOI: 10.5604/01.3001.0010.5267

Adam Sokal, Sylwia Wójcik, Patrycja Pruszkowska, Oskar Kowalski, Radoslaw Lenarczyk, Agnieszka Liberska, Michał Mazurek, Slawomir Pluta, Zbigniew Kalarus

{"title":"Ferritin as a potential biomarker of efficacy of treatment of atrial fibrillation - preliminary report.","authors":"Adam Sokal, Sylwia Wójcik, Patrycja Pruszkowska, Oskar Kowalski, Radoslaw Lenarczyk, Agnieszka Liberska, Michał Mazurek, Slawomir Pluta, Zbigniew Kalarus","doi":"10.5604/01.3001.0010.5267","DOIUrl":"https://doi.org/10.5604/01.3001.0010.5267","url":null,"abstract":"Background: Complex mechanisms of responsible for originating and maintaining of atrial fibrillation (AF) are involved in pathophysiology of this arrhythmia. Inflammation substantially contribute to arrhythmic remodelling of atrial tissue.The aim of the present study is to assess an applicability of ferritin and high sensitive C-reactive protein (hs-CRP) as biomarkers of atrial fibrillation and their usefulness in evaluation of efficacy of cryoablation.Materials and methods: The study population consisted of 40 patients who underwent first AF cryoablation procedure. The whole follow-up time was for 6 months. The efficacy of cryoablation was defined as lack of episodes of AF longer than 30 s reported either in patient's medical documentation or present in standard or Holter ECG records. Concentrations of hs-CRP (latex method ) and ferritin (immunochemical method) were determined in standard way in hospital laboratory.Results: The recurrence of atrial fibrillation during follow-up was detected in 7 of 40 patients (efficacy 82.5%). Basal concentrations of hs-CRP and ferritin were significantly higher in patients who underwent ablation during AF. Ablation resulted in an increase of either hs-CRP or ferritin concentrations. After seven days, both hs-CRP and ferritin concentrations returned to basal level. The trend toward the higher concentration of hs-CRP was observed in AF recurrence subgroup in 30th and the 90th day after the procedure. Ferritin concentrations were significantly higher in recurrence subgroup after 30 and 90 days.Conclusion: Our results suggest that the evaluation of ferritin serum level can be a potential tool for assessment of AF treatment efficacy.","PeriodicalId":87132,"journal":{"name":"Postepy higieny i medycyny doswiadczalnej (Online)","volume":"71 0","pages":"876-880"},"PeriodicalIF":0.0,"publicationDate":"2017-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35457383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Non-nutritional use of breast milk. 无营养地使用母乳。

Postepy higieny i medycyny doswiadczalnej (Online) Pub Date : 2017-10-04 DOI: 10.5604/01.3001.0010.5049

Magdalena Maria Piskorska-Jasiulewicz, Małgorzata Witkowska-Zimny

引用次数: 3