ASN NEURO最新文献_第6页

Microglia at the Crossroads of Pathogen-Induced Neuroinflammation 处于病原体诱导的神经炎症十字路口的小胶质细胞

IF 4.7 4区医学

ASN NEURO Pub Date : 2022-01-01 DOI: 10.1177/17590914221104566

A. M. Rodríguez, J. Rodríguez, G. Giambartolomei

引用次数: 20

Defibrinogenation Ameliorates Retinal Microgliosis and Inflammation in A CX3CR1-Independent Manner. 去纤维蛋白原能以一种与 CX3CR1 无关的方式改善视网膜小胶质细胞增多和炎症。

IF 3.9 4区医学

ASN NEURO Pub Date : 2022-01-01 DOI: 10.1177/17590914221131446

Borna Sarker, Sandra M Cardona, Kaira A Church, Difernando Vanegas, Priscila Velazquez, Colin Rorex, Derek Rodriguez, Andrew S Mendiola, Timothy S Kern, Nadia D Domingo, Robin Stephens, Isabel A Muzzio, Astrid E Cardona

引用次数: 0

Neural Stem Cells in Adult Mammals are not Astrocytes. 成年哺乳动物的神经干细胞不是星形胶质细胞

IF 3.9 4区医学

ASN NEURO Pub Date : 2022-01-01 DOI: 10.1177/17590914221134739

Fernando Janczur Velloso, Sandhya Shankar, Vladimir Parpura, Pasko Rakic, Steven W Levison

{"title":"Neural Stem Cells in Adult Mammals are not Astrocytes.","authors":"Fernando Janczur Velloso, Sandhya Shankar, Vladimir Parpura, Pasko Rakic, Steven W Levison","doi":"10.1177/17590914221134739","DOIUrl":"10.1177/17590914221134739","url":null,"abstract":"At the turn of the 21st century studies of the cells that resided in the adult mammalian subventricular zone (SVZ) characterized the neural stem cells (NSCs) as a subtype of astrocyte. Over the ensuing years, numerous studies have further characterized the properties of these NSCs and compared them to parenchymal astrocytes. Here we have evaluated the evidence collected to date to establish whether classifying the NSCs as astrocytes is appropriate and useful. We also performed a meta-analysis with 4 previously published datasets that used cell sorting and unbiased single-cell RNAseq to highlight the distinct gene expression profiles of adult murine NSCs and niche astrocytes. On the basis of our understanding of the properties and functions of astrocytes versus the properties and functions of NSCs, and from our comparative transcriptomic analyses we conclude that classifying the adult mammalian NSC as an astrocyte is potentially misleading. From our vantage point, it is more appropriate to refer to the cells in the adult mammalian SVZ that retain the capacity to produce new neurons and macroglia as NSCs without attaching the term \"astrocyte-like.\"","PeriodicalId":8616,"journal":{"name":"ASN NEURO","volume":"14 ","pages":"17590914221134739"},"PeriodicalIF":3.9,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ba/3b/10.1177_17590914221134739.PMC9638700.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9787401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Müller Cell Molecular Heterogeneity: Facts and Predictions 米勒细胞分子异质性：事实与预测

IF 4.7 4区医学

ASN NEURO Pub Date : 2022-01-01 DOI: 10.1177/17590914221106903

M. Lamas, Erick J. Martinez-Colin

{"title":"Müller Cell Molecular Heterogeneity: Facts and Predictions","authors":"M. Lamas, Erick J. Martinez-Colin","doi":"10.1177/17590914221106903","DOIUrl":"https://doi.org/10.1177/17590914221106903","url":null,"abstract":"The retina was historically considered as an “approachable part of the brain”; advantageous, for its simplicity, to use as a model organ for deciphering cellular and molecular mechanisms underlying physiology and pathology of the nervous system. However, the most relevant discoveries arise precisely from unveiling the complexity of the retina. A complexity that partially relies on the layered organization of an extended variety of specialized neuronal and glial cellular types and subtypes. Based on functional, morphological or transcriptome data, over 40 subtypes of retinal ganglion cells or 60 subtypes of retinal amacrine cells have been described. A high degree of specialization, that may lead to segregation into functionally diverse subtypes, is also conceivable for Müller cells, a pleiotropic glial component of all vertebrate retinas. The essential role of Müller glia in retinal homeostasis maintenance involves participation in structural, metabolic and intercellular communication processes. Additionally, they are the only retinal cells that possess regenerative potential in response to injury or disease, and thus may be considered as therapeutic tools. In the assumption that functional heterogeneity might be driven by molecular heterogeneity this review aims to compile emerging evidence that could broaden our understanding of Müller cell biology and retinal physiology. Summary statement Müller glial cells exert multiple essential functions in retinal physiology and retinopathies reflecting perhaps the existence of distinct Müller cellular subpopulations. Harnessing Müller cell heterogeneity may serve to enhance new therapeutic approaches for retinal disease.","PeriodicalId":8616,"journal":{"name":"ASN NEURO","volume":" ","pages":""},"PeriodicalIF":4.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43324845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Astrocyte Cell Surface Antigen 2 and Other Potential Cell Surface Markers of Enteric glia in the Mouse Colon. 小鼠结肠肠胶质细胞星形胶质细胞表面抗原2和其他潜在的细胞表面标记物。

IF 4.7 4区医学

ASN NEURO Pub Date : 2022-01-01 DOI: 10.1177/17590914221083203

Vladimir Grubišić, Brian D Gulbransen

{"title":"Astrocyte Cell Surface Antigen 2 and Other Potential Cell Surface Markers of Enteric glia in the Mouse Colon.","authors":"Vladimir Grubišić, Brian D Gulbransen","doi":"10.1177/17590914221083203","DOIUrl":"https://doi.org/10.1177/17590914221083203","url":null,"abstract":"Enteric glia regulate gut functions in health and disease through diverse interactions with neurons and immune cells. Intracellular localization of traditional markers of enteric glia such as GFAP, s100b, and Sox10 makes them incompatible for studies that require antigen localization at the cell surface. Thus, new tools are needed for probing the heterogeneous roles of enteric glia at the protein, cell, and functional levels. Here we selected several cell surface antigens including Astrocyte Cell Surface Marker 2 (ACSA2), Cluster of differentiation 9 (CD9), lysophosphatidic acid receptor 1 (LPAR1), and Proteolipid protein 1 (PLP1) as potential markers of enteric glia. We tested their specificity for enteric glia using published single-cell/-nuclei and glia-specific translating mRNA enriched transcriptome datasets, immunolabeling, and flow cytometry. The data show that ACSA2 is a specific marker of mucosal and myenteric glia while other markers are suitable for identifying all subpopulations of enteric glia (LPAR1), glia and immune cells (CD9), or are not suitable for cell-surface labeling (PLP1). These new tools will be useful for future work focused on understanding specific glial functions in health and disease.Summary StatementThis study identifies astrocyte cell surface antigen 2 as a novel marker of myenteric glia in the intestine. This, in combination with other markers identified in this study, could be used for selective targeting of enteric glia.","PeriodicalId":8616,"journal":{"name":"ASN NEURO","volume":"14 ","pages":"17590914221083203"},"PeriodicalIF":4.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/0f/23/10.1177_17590914221083203.PMC9125112.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9302672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rab11 and Its Role in Neurodegenerative Diseases. Rab11及其在神经退行性疾病中的作用

IF 4.7 4区医学

ASN NEURO Pub Date : 2022-01-01 DOI: 10.1177/17590914221142360

Pinky Sultana, Jiri Novotny

{"title":"Rab11 and Its Role in Neurodegenerative Diseases.","authors":"Pinky Sultana, Jiri Novotny","doi":"10.1177/17590914221142360","DOIUrl":"https://doi.org/10.1177/17590914221142360","url":null,"abstract":"Vesicles mediate the trafficking of membranes/proteins in the endocytic and secretory pathways. These pathways are regulated by small GTPases of the Rab family. Rab proteins belong to the Ras superfamily of GTPases, which are significantly involved in various intracellular trafficking and signaling processes in the nervous system. Rab11 is known to play a key role especially in recycling many proteins, including receptors important for signal transduction and preservation of functional activities of nerve cells. Rab11 activity is controlled by GEFs (guanine exchange factors) and GAPs (GTPase activating proteins), which regulate its function through modulating GTP/GDP exchange and the intrinsic GTPase activity, respectively. Rab11 is involved in the transport of several growth factor molecules important for the development and repair of neurons. Overexpression of Rab11 has been shown to significantly enhance vesicle trafficking. On the other hand, a reduced expression of Rab11 was observed in several neurodegenerative diseases. Current evidence appears to support the notion that Rab11 and its cognate proteins may be potential targets for therapeutic intervention. In this review, we briefly discuss the function of Rab11 and its related interaction partners in intracellular pathways that may be involved in neurodegenerative processes.","PeriodicalId":8616,"journal":{"name":"ASN NEURO","volume":"14 ","pages":"17590914221142360"},"PeriodicalIF":4.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6f/55/10.1177_17590914221142360.PMC9726856.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10342452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Disruption of Synaptic Transmission in the Bed Nucleus of the Stria Terminalis Reduces Seizure-Induced Death in DBA/1 Mice and Alters Brainstem E/I Balance 终止纹状体床核突触传递的破坏减少了DBA/1小鼠癫痫诱导的死亡并改变了脑干E/I平衡

IF 4.7 4区医学

ASN NEURO Pub Date : 2021-12-24 DOI: 10.1101/2021.12.23.473665

Maya Y. Xia, Benjamin Owen, J. Chiang, Alyssa Levitt, Katherine Preisinger, W. Yan, Ragan Huffman, W. Nobis

{"title":"Disruption of Synaptic Transmission in the Bed Nucleus of the Stria Terminalis Reduces Seizure-Induced Death in DBA/1 Mice and Alters Brainstem E/I Balance","authors":"Maya Y. Xia, Benjamin Owen, J. Chiang, Alyssa Levitt, Katherine Preisinger, W. Yan, Ragan Huffman, W. Nobis","doi":"10.1101/2021.12.23.473665","DOIUrl":"https://doi.org/10.1101/2021.12.23.473665","url":null,"abstract":"Sudden unexpected death in epilepsy (SUDEP) is the leading cause of death in refractory epilepsy patients. Accumulating evidence from recent human studies and animal models suggests that seizure-related respiratory arrest may be important for initiating cardiorespiratory arrest and death. Prior evidence suggests that apnea onset can coincide with seizure spread to the amygdala and that stimulation of the amygdala can reliably induce apneas in epilepsy patients, potentially implicating amygdalar regions in seizure-related respiratory arrest and subsequent postictal hypoventilation and cardiorespiratory death. This study aimed to determine if an extended amygdalar structure, the dorsal bed nucleus of the stria terminalis (dBNST), is involved in seizure-induced respiratory arrest (S-IRA) and death using DBA/1 mice, a mouse strain which has audiogenic seizures (AGS) and a high incidence of postictal respiratory arrest and death. The presence of S-IRA significantly increased c-Fos expression in the dBNST of DBA/1 mice. Furthermore, disruption of synaptic output from the dBNST via viral-induced tetanus neurotoxin (TeNT) significantly improved survival following S-IRA in DBA/1 mice without affecting baseline breathing or hypercapnic (HCVR) and hypoxic ventilatory response (HVR). This disruption in the dBNST resulted in changes to the balance of excitatory/inhibitory (E/I) synaptic events in the downstream brainstem regions of the lateral parabrachial nucleus (PBN) and the periaqueductal gray (PAG). These findings suggest that the dBNST is a potential subcortical forebrain site necessary for the mediation of S-IRA, potentially through its outputs to brainstem respiratory regions.","PeriodicalId":8616,"journal":{"name":"ASN NEURO","volume":"14 1","pages":""},"PeriodicalIF":4.7,"publicationDate":"2021-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44908187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

2021 ASN Virtual Meeting Abstracts. 2021 ASN虚拟会议摘要。

IF 4.7 4区医学

ASN NEURO Pub Date : 2021-01-01 DOI: 10.1177/17590914211039028

引用次数: 0

Characterization of the Expression of Vacuolar Protein Sorting 11 (Vps11) in Mammalian Oligodendrocytes. 空泡蛋白分选11 (Vps11)在哺乳动物少突胶质细胞中的表达

IF 4.7 4区医学

ASN NEURO Pub Date : 2021-01-01 DOI: 10.1177/17590914211009851

Robert P Skoff, Denise Bessert, Shreya Banerjee, Xixia Luo, Ryan Thummel

{"title":"Characterization of the Expression of Vacuolar Protein Sorting 11 (Vps11) in Mammalian Oligodendrocytes.","authors":"Robert P Skoff, Denise Bessert, Shreya Banerjee, Xixia Luo, Ryan Thummel","doi":"10.1177/17590914211009851","DOIUrl":"https://doi.org/10.1177/17590914211009851","url":null,"abstract":"A founder mutation in human VPS11 (Vacuolar Protein Sorting 11) was recently linked to a genetic leukoencephalopathy in Ashkenazi Jews that presents with the classical features of white matter disorders of the central nervous system (CNS). The neurological deficits include hypomyelination, hypotonia, gradual loss of vision, and seizures. However, the cells expressing the mutation were not identified. Here we describe, using immunocytochemistry, the strong expression of Vps11 in mouse oligodendrocytes and, specifically, its localization with Myelin Associated Glycoprotein (MAG) in the inner tongue of myelin. In longitudinal sections of myelin, it forms a bead-like structure, alternating with Myelin Basic Protein (MBP). Immunofluorescent staining with Vps11 and neurofilament proteins indicates the absence of Vps11 in axons in vivo. Finally, changes in Vps11 expression are associated with altered proteolipid protein (PLP) levels based upon mice with duplications or deletions of the Plp1 gene. To determine potential functional contributions of Vps11, we combined Vps11 with Platelet Derived Growth Factor Receptor-α (PDGFRα) in vitro and in vivo: in both conditions, co-localization of the two proteins was frequently found in round vesicles of OPCs/oligodendrocytes, suggesting retrograde transport for degradation by the endolysosomal system. Neuron-to-glial communication has been invoked to explain degenerative changes in myelin followed by degenerative changes in axons, and vice versa; but to our knowledge, no specific proteins in retrograde transport from the myelin inner tongue to oligodendrocyte perikarya have been identified. The identification of mutations in VPS11 and its localization at the axon-myelin interface should open new avenues of research.","PeriodicalId":8616,"journal":{"name":"ASN NEURO","volume":"13 ","pages":"17590914211009851"},"PeriodicalIF":4.7,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/17590914211009851","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10077084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Human Umbilical Cord Mesenchymal Stem Cells-Secreted TSG-6 Is Anti-Inflammatory and Promote Tissue Repair After Spinal Cord Injury 人脐带间充质干细胞分泌TSG-6抗炎促进脊髓损伤后组织修复

IF 4.7 4区医学

ASN NEURO Pub Date : 2021-01-01 DOI: 10.1177/17590914211010628

Ziling Liao, Wei Wang, Weiyue Deng, Yuying Zhang, Aishi Song, Sihao Deng, Huifang Zhao, Shusheng Zhang, Zhiyuan Li

{"title":"Human Umbilical Cord Mesenchymal Stem Cells-Secreted TSG-6 Is Anti-Inflammatory and Promote Tissue Repair After Spinal Cord Injury","authors":"Ziling Liao, Wei Wang, Weiyue Deng, Yuying Zhang, Aishi Song, Sihao Deng, Huifang Zhao, Shusheng Zhang, Zhiyuan Li","doi":"10.1177/17590914211010628","DOIUrl":"https://doi.org/10.1177/17590914211010628","url":null,"abstract":"Spinal cord injury (SCI) causes patients paralysis and hard to recover. The therapeutic effects of current clinical drugs are accompanied by side effects. In recent years, stem cell therapy has attracted the attention of researchers. Human umbilical cord mesenchymal stem cells (hucMSCs) have been widely used in various diseases due to their excellent paracrine function. TNF-stimulated gene 6 (TSG-6), a secretion factor of stem cells, may play an important role in hucMSCs in the treatment of SCI. So we conducted an experiment to explore its effect. We first observed that the expression of TSG-6 increased in SCI rats after injected with hucMSCs. Then, we used siRNA to knowdown the expression of TSG-6. We treated SCI rats with TSG-6-knockdown hucMSCs. Without TSG-6 expression, hucMSCs treatment made the tissue recovery worse and the number of Nissl bodies less. Meanwhile, neutrophils infiltrated more in the damaged parts. Our research also proved that TSG-6 may help demyelination recovering and alleviate astrocytes gathering in the injury sites. Our study revealed that hucMSCs secreted TSG-6 may decrease the degeneration of myelin sheath, reduce inflammation, decrease neuron loss and promote tissue repair. These results provided a new therapeutic factor for the treatment of SCI.","PeriodicalId":8616,"journal":{"name":"ASN NEURO","volume":"13 1","pages":""},"PeriodicalIF":4.7,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1177/17590914211010628","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45142851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3