Australasian Plant Pathology最新文献_第5页

First report of ‘Candidatus Phytoplasma asteris’ related strain associated with potato purple top disease complex in Brazil 巴西首次报道与马铃薯紫顶病复合体相关的“星形候选植物原体”相关菌株

IF 1.2 4区农林科学

Australasian Plant Pathology Pub Date : 2026-06-23 DOI: 10.1007/s13313-026-01161-7

Jacson Ferreira, Antonia Fabiana Pinto de Holanda, Diana Gissel Barreto Ramos, Ivan Paulo Bedendo

引用次数: 0

Occurrence of stem canker in pitaya orchards (Hylocereus spp.) caused by Neoscytalidium dimidiatum in the Western Ghats, India 印度西高止山脉火龙果（Hylocereus spp.）果茎溃疡病的发生

IF 1.2 4区农林科学

Australasian Plant Pathology Pub Date : 2026-06-22 DOI: 10.1007/s13313-026-01163-5

R. S. Giriprasath, B. C. Umesh, B. Madhumitha, Sajan Kurien, A. Bharanidharan

{"title":"Occurrence of stem canker in pitaya orchards (Hylocereus spp.) caused by Neoscytalidium dimidiatum in the Western Ghats, India","authors":"R. S. Giriprasath, B. C. Umesh, B. Madhumitha, Sajan Kurien, A. Bharanidharan","doi":"10.1007/s13313-026-01163-5","DOIUrl":"10.1007/s13313-026-01163-5","url":null,"abstract":"<div>Hylocereus spp, commonly known as the pitaya, pitahaya or dragon fruit, has substantial nutritional and medicinal significance. From 2025 to 2026, pitaya plants in the orchard of Western Ghats (Coimbatore) exhibited symptoms of stem canker. Initially minor, circular, chlorotic, pitted spots that gradually developed into reddish-brown flecks were observed. Based on morphological characteristics and molecular sequence of the internal transcribed spacer (ITS) region, the pathogen was identified as Neoscytalidium dimidiatum. BLASTn analyses revealed 99–100% sequence similarity with N. dimidiatum accessions in GenBank. Phylogenetic analysis grouped the isolates with N. dimidiatum, providing strong molecular evidence for identification. Koch postulates under controlled conditions confirmed the causal agent of the disease. This is the first report of stem canker caused by N. dimidiatum. These findings offer new insights into stem canker affecting pitaya and highlight the importance of executing effective disease management strategies to minimize the economic impact of this disease on pitaya cultivation in Tamil Nadu.</div>","PeriodicalId":8598,"journal":{"name":"Australasian Plant Pathology","volume":"55 4","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148323507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Deciphering genotype × environment interactions to assess durable resistance against the blight complex in lentil 破译基因型与环境相互作用以评估扁豆对疫病复合体的持久抗性

IF 1.2 4区农林科学

Australasian Plant Pathology Pub Date : 2026-06-19 DOI: 10.1007/s13313-026-01160-8

Huma Nazneen, Raju Das, Arpita Das, Sudip Bhattacharya, Soumyayan Roy, Subrata Dutta, Kuldeep Tripathi, Sanjeev Gupta, Shiv Kumar

{"title":"Deciphering genotype × environment interactions to assess durable resistance against the blight complex in lentil","authors":"Huma Nazneen, Raju Das, Arpita Das, Sudip Bhattacharya, Soumyayan Roy, Subrata Dutta, Kuldeep Tripathi, Sanjeev Gupta, Shiv Kumar","doi":"10.1007/s13313-026-01160-8","DOIUrl":"10.1007/s13313-026-01160-8","url":null,"abstract":"<div>Lentil is a highly nutritious legume essential for global food security and rising global demand. Lentil cultivation is severely impeded by the blight complex inflicted by Stemphylium botryosum and Alternaria alternata. This intricate disease is mostly prevalent in humid subtropical regions and is often exacerbated by climate change. Co-infections complicate pathogen identification and hinder effective management strategies. Existing research has primarily focused on resistance to individual pathogens, highlighting a research gap in addressing combined resistance to both pathogens. Therefore, the present study was conducted to elucidate the impact of genotype-environment interactions on disease dynamics across diverse locations over multiple years and to identify durable resistant genotypes for sustainable lentil production in blight-affected areas. Preliminary screening was conducted on 268 lentil genotypes. Subsequently, a panel of 25 promising genotypes, including susceptible checks, were further evaluated over three consecutive years at three locations in the Gangetic Alluvial and Red lateritic zones. Substantial variation was observed, with a significant impact of environment and genotype-environment interaction on the variable genotypic response, thus validating the preponderance of non-cross-over interaction and the urgency to detect durable resistance sources. The complementary use of BLUP-based predictions and GGE biplot visualisation precisely identified IC559983 as the most stable genotype with a moderately resistant response, and TCADL 19–5 as a desirable genotype with a consistent resistant response across the tested locations. The genotypes identified in this study offer valuable resources for effective management of the blight complex, with the potential to enhance lentil productivity and contribute to food security.</div>","PeriodicalId":8598,"journal":{"name":"Australasian Plant Pathology","volume":"55 4","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148281776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Occurrence and molecular identification of Plantago tobamovirus 1 (Genus Tobamovirus) in Saffron (Crocus sativus L.) in Iran 伊朗藏红花（Crocus sativus L.）中车前子多巴病毒1型（totabago tobamovirus 1）的发生及分子鉴定

IF 1.2 4区农林科学

Australasian Plant Pathology Pub Date : 2026-06-16 DOI: 10.1007/s13313-026-01150-w

Akram Tavakoli Bardaskan, Seyyedeh Atefeh Hosseini, Charlotte Julian

{"title":"Occurrence and molecular identification of Plantago tobamovirus 1 (Genus Tobamovirus) in Saffron (Crocus sativus L.) in Iran","authors":"Akram Tavakoli Bardaskan, Seyyedeh Atefeh Hosseini, Charlotte Julian","doi":"10.1007/s13313-026-01150-w","DOIUrl":"10.1007/s13313-026-01150-w","url":null,"abstract":"<div>Saffron (Crocus sativus L.) plays a vital role in Iran's agricultural economy; however, viral infections pose a serious threat to its sustainable production. In this study, the presence ofPlantago tobamovirus 1 (PTV1) was identified and reported for the first time in Iranian saffron fields. Using virion-associated nucleic acid (VANA)–based metagenomics and 454 pyrosequencing, the virome of 161 leaf samples collected from three major saffron cultivation provinces was investigated. Approximately 74% of the reads were of viral origin, and sequences belonging to the family Tobamoviridae with 99.2–92.9% nucleotide identity to PTV1 were identified. Ten saffron samples tested positive for PTV1, and the presence of the virus was confirmed in three samples by RT-PCR and Sanger sequencing. Phylogenetic analyses based on the movement protein (MP) and RNA-dependent RNA polymerase (RdRp) genes revealed that the Iranian isolates clustered within the PTV1 clade but formed a distinct subclade, indicating their genetic divergence from reference isolates. The highest nucleotide identity of the Iranian isolate with the American isolate (OP594471) was approximately 98% for the RdRp gene and about 90% for the MP gene. A dN/dS ratio of less than one suggested the action of purifying selection on both genes. These findings provide the first molecular evidence of PTV1 infection in saffron in Iran and emphasize the necessity for monitoring emerging viruses and the role of weeds as potential reservoirs in the epidemiology of the virus.</div>","PeriodicalId":8598,"journal":{"name":"Australasian Plant Pathology","volume":"55 4","pages":""},"PeriodicalIF":1.2,"publicationDate":"2026-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148281025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Draft genome sequence of Ganoderma philippii, a serious root rot pathogen of Eucalyptus in Southeast Asia 绘制东南亚桉树严重根腐病病原菌灵芝（Ganoderma philippii）基因组序列

IF 1.1 4区农林科学

Australasian Plant Pathology Pub Date : 2026-06-10 DOI: 10.1007/s13313-026-01159-1

Alishia van Heerden, Nam Q. Pham, Tuan A. Duong, Michael J. Wingfield, Brenda D. Wingfield

{"title":"Draft genome sequence of Ganoderma philippii, a serious root rot pathogen of Eucalyptus in Southeast Asia","authors":"Alishia van Heerden, Nam Q. Pham, Tuan A. Duong, Michael J. Wingfield, Brenda D. Wingfield","doi":"10.1007/s13313-026-01159-1","DOIUrl":"10.1007/s13313-026-01159-1","url":null,"abstract":"The root-rot fungus Ganoderma philippii is an important pathogen of forest trees, especially in Southeast Asian countries such as Indonesia and Malaysia. The growing importance of this pathogen, especially on plantation-grown trees such as species of Acacia and Eucalyptus has required whole genome sequence data to better understand its biology. In this study, we present the draft genome sequence of a G. philippii isolate sequenced using Oxford Nanopore technology. The assembled genome was 53.49 Mb in size with an N50 of 200.66 kb, and GC content of 54.77%. Genome completeness was assessed, revealing 95.0% completeness for the BUSCO polyporales_odb10 database. GeneMark-ES predicted a total of 9,277 protein coding genes and phylogenetic inference based on ITS sequence confirmed the identity of the sequenced isolate as G. philippii. This study provided the first openly available genome of G. philippii that will be an important resource for developing molecular tools for population and evolutionary studies, pathogen diagnostics and will facilitate comparative analysis among different Ganoderma species.","PeriodicalId":8598,"journal":{"name":"Australasian Plant Pathology","volume":"55 4","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148236639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biological characterization and complete genome analysis of Tobamovirus fructirugosum from Türkiye <s:1>基耶果状托巴莫病毒生物学特性及全基因组分析

IF 1.1 4区农林科学

Australasian Plant Pathology Pub Date : 2026-06-03 DOI: 10.1007/s13313-026-01157-3

Derya Demiralan, Eminur Elçi

{"title":"Biological characterization and complete genome analysis of Tobamovirus fructirugosum from Türkiye","authors":"Derya Demiralan, Eminur Elçi","doi":"10.1007/s13313-026-01157-3","DOIUrl":"10.1007/s13313-026-01157-3","url":null,"abstract":"<div>Tomato brown rugose fruit virus (Tobamovirus fructirugosum, ToBRFV), a member of the Tobamovirus genus, represents a significant threat to economically important Solanaceous crops. This study investigated ToBRFV in tomato and pepper, encompassing the following: molecular detection and identification of the virus; symptom development in susceptible hosts; screening of varieties for the presence of the Tm-22 gene, a known Tobamovirus resistance gene; and complete genome sequencing and analysis for comprehensive genetic characterization of ToBRFV isolates. In 2024, tomato and pepper varieties were mechanically inoculated with ToBRFV, and disease severity was assessed. The symptom development was both rapid and severe, with certain varieties demonstrating a high degree of susceptibility, while others exhibited sensitivity, and a single variety demonstrated tolerance. Tetra-primer Amplification Refractory Mutation System (ARMS) co-dominant markers were used to investigate the Tm-22 resistance gene. Surprisingly, some varieties carrying resistance genes still developed viral infections. Double-stranded RNA (dsRNA) was extracted from infected plants and subjected to high-throughput sequencing (HTS) for complete genome analysis. For the validation of HTS results, primer pairs targeting overlapping regions spanning the complete ToBRFV genome, were designed and employed in reverse transcription polymerase chain reaction (RT-PCR) analysis, followed by Sanger sequencing. The ToBRFV RNA-dependent RNA polymerase (RdRp) gene of selected isolates was partially analyzed. Furthermore, phylogenetic and recombination analyses were conducted. The results confirm the highly stable and contagious nature of ToBRFV. These findings contribute to a better understanding of ToBRFV diversity and resistance, providing valuable information for the development of virus management strategies.</div>","PeriodicalId":8598,"journal":{"name":"Australasian Plant Pathology","volume":"55 4","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13313-026-01157-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148172275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic variation and race structure in the tan spot wheat pathogen Pyrenophora tritici-repentis: a microscale assessment in a wheat field of Western Australia 小麦褐斑病菌的遗传变异和小种结构：西澳大利亚麦田的微尺度评价

IF 1.1 4区农林科学

Australasian Plant Pathology Pub Date : 2026-05-29 DOI: 10.1007/s13313-026-01158-2

Pao Theen See, Catherine F. Cupitt, Dion Bennett, Caroline S. Moffat, Simon R. Ellwood

{"title":"Genetic variation and race structure in the tan spot wheat pathogen Pyrenophora tritici-repentis: a microscale assessment in a wheat field of Western Australia","authors":"Pao Theen See, Catherine F. Cupitt, Dion Bennett, Caroline S. Moffat, Simon R. Ellwood","doi":"10.1007/s13313-026-01158-2","DOIUrl":"10.1007/s13313-026-01158-2","url":null,"abstract":"<div>Population genetics are routinely studied over vast geographical areas to address questions of origin, routes of migration and epidemiology. However, genetic diversity of certain microorganisms such as plant pathogenic fungi are difficult to study even on a small geographical scale due to their mixed reproduction systems, variable population sizes, clonality and versatility to host adaptation. The genetic variation of an economically important wheat fungal pathogen, Pyrenophora tritici-repentis (Ptr), was studied with isolates collected from a naturally infected wheat field located in Western Australia, using microsatellite (SSR) markers. Out of 142 Ptr isolates, 89 individuals showed unique multilocus haplotypes. On single leaves, individual disease lesions mostly consisted of genetically variable Ptr isolates, with very few clonal isolates. Distribution analysis of these indicated that wind-dispersal of Ptr conidia occurred over a small scale within individual leaves as well as longer dispersions of up to 45 m. Race evaluation of the population via effector gene PCR assays defined 136 isolates as race 1 and six as race 2, and there was no indication of genetic groupings based on micro-locations or race. Population structure analysis suggests that the population consists of mixed migrants from long wind-dispersal or field management activities. Nucleotide sequence analysis of the homothallic mating-type genes, MAT1-1 and MAT1-2 revealed sequence conservation within the population. Altogether, the microscale evaluation of the single wheat field isolates demonstrated that the population is suitable for disease screening as it is representative of the wider Ptr population demographic in Australia.</div>","PeriodicalId":8598,"journal":{"name":"Australasian Plant Pathology","volume":"55 4","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13313-026-01158-2.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148173327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A 16 S rRNA- targeted droplet digital PCR (ddPCR) assay for sensitive detection and absolute quantification of phytoplasmas 采用16s rRNA靶向滴滴数字PCR （ddPCR）技术对植物原体进行灵敏检测和绝对定量

IF 1.1 4区农林科学

Australasian Plant Pathology Pub Date : 2026-05-23 DOI: 10.1007/s13313-026-01155-5

Yigit Sabri Unlu, Isil Tulum

{"title":"A 16 S rRNA- targeted droplet digital PCR (ddPCR) assay for sensitive detection and absolute quantification of phytoplasmas","authors":"Yigit Sabri Unlu, Isil Tulum","doi":"10.1007/s13313-026-01155-5","DOIUrl":"10.1007/s13313-026-01155-5","url":null,"abstract":"<div>Phytoplasmas are phloem-restricted, insect-transmitted plant pathogens responsible for destructive diseases in numerous crops. Their obligate parasitism, inability to be cultured in vitro, and typically low titers in infected tissues create a critical need for highly sensitive and reliable diagnostic tools. Here, we developed and validated a droplet digital PCR (ddPCR) assay targeting a conserved region of the 16 S rRNA gene for broad-range detection and absolute quantification of phytoplasmas. Using the UniRNA primer/probe set, the assay’s specificity was confirmed through in silico analyses and multiple sequence alignments against various phytoplasma ribosomal groups and non-target pathogens. Linearity was observed across a broad dynamic range, detecting 55-1576 copies µl−¹ from serial dilutions of naturally infected tomato DNA. Probit regression analysis demonstrated that ddPCR outperformed qPCR in sensitivity- exhibiting a 5.82-fold lower detection limit (LOD95 of 38.41 copies/reaction)- and provided more robust quantification for low-titer samples. Field-collected symptomatic tomato plants were identified as ‘Candidatus Phytoplasma trifolii’ through nested PCR, sequencing, and virtual RFLP analyses. The developed ddPCR system also successfully detected multiple reference phytoplasma groups, validating its broad applicability. This broad range ddPCR assay enhances early disease detection, supports accurate surveillance, and offers a robust diagnostic tool for pathogen monitoring and phytosanitary programs.</div>","PeriodicalId":8598,"journal":{"name":"Australasian Plant Pathology","volume":"55 4","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148009199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection and infectivity analysis of tomato yellow leaf curl virus and its associated satellites in guar and sunflower 瓜尔豆和向日葵中番茄黄卷叶病毒及其伴生卫星的检测和传染性分析

IF 1.1 4区农林科学

Australasian Plant Pathology Pub Date : 2026-05-22 DOI: 10.1007/s13313-026-01153-7

Asra Salari, Jahangir Heydarnejad, Maryam Esmaeili

{"title":"Detection and infectivity analysis of tomato yellow leaf curl virus and its associated satellites in guar and sunflower","authors":"Asra Salari, Jahangir Heydarnejad, Maryam Esmaeili","doi":"10.1007/s13313-026-01153-7","DOIUrl":"10.1007/s13313-026-01153-7","url":null,"abstract":"<div>Tomato yellow leaf curl virus (TYLCV; Begomovirus coheni) is a highly destructive plant virus, especially in tropical and subtropical regions worldwide. This study detected TYLCV and its associated satellites‒Gossypium darwinii symptomless alphasatellite (GDarSLA, Colecusatellite gossypiumdarwinii) and/or tomato leaf curl betasatellite (ToLCB, Betasatellite solani)‒in symptomatic guar and sunflower samples from Jiroft, southeastern Iran. Full-length sequences of two TYLCV isolates shared 89.1% nucleotide identity with each other and 85.5–99.45% with other Iranian isolates from various crops available in GenBank. Furthermore, GDarSLA and ToLCB satellites shared 93.4–99.9% and 95.6–96.8% nucleotide sequence identities with other Iranian isolates recovered from different crops. Agroinfiltration of guar and sunflower using a previously constructed infectious clone of the virus and/or the associated betasatellite (TYLCV or TYLCV + ToLCB) resulted in symptomless TYLCV infection; however, the betasatellite was not detected in both plants. Inoculation of tomato with the same constructs induced symptom of varying severity depending on the presence of ToLCB in the inoculation tests. The phenotypic differences observed in infected guar and sunflower under natural versus experimental conditions are discussed. This study identifies sunflower as a new TYLCV host. Considering the limited cultivation of both guar and sunflower in Jiroft, climatic conditions of this region promote continuous exposure of various plants to TYLCV and the associated satellites.</div>","PeriodicalId":8598,"journal":{"name":"Australasian Plant Pathology","volume":"55 4","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148009414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fusarium redolens, the primary causal agent of wilt and yellows of chickpea in western Iran 伊朗西部鹰嘴豆枯萎病和黄病的主要病原——红镰刀菌

IF 1.1 4区农林科学

Australasian Plant Pathology Pub Date : 2026-05-20 DOI: 10.1007/s13313-026-01154-6

Saeed Abbasi, Gelareh Zarbanoei, Kianoosh Cheghamirza, Leila Zarei, Elham Seidmohammadi

{"title":"Fusarium redolens, the primary causal agent of wilt and yellows of chickpea in western Iran","authors":"Saeed Abbasi, Gelareh Zarbanoei, Kianoosh Cheghamirza, Leila Zarei, Elham Seidmohammadi","doi":"10.1007/s13313-026-01154-6","DOIUrl":"10.1007/s13313-026-01154-6","url":null,"abstract":"<div>Chickpea is a valuable crop with high nutritional value. It also helps maintain soil fertility, especially in dry and semi-arid areas such as Iran, where it is cultivated in crop rotation with cereals. Wilt and root rot is a devastating disease commonly occurring during all growing seasons in Iran and inflicts significant yield losses on farmers. In all previous studies, Fusarium oxysporum f. sp. ciceris has been reported as the leading cause of the disease in Iran. However, in most previous studies, identification of the pathogen was based exclusively on morphological characteristics, and isolates of F. oxysporum species complex (FOSC) were considered as the forma specialis of F. oxysporum f. sp. ciceris, simply because of their association with wilt-like symptoms. In a recent study, FOSC was still the predominant pathogenic Fusarium species on chickpea in Iran. However, most affected plants showed no discoloration in the vascular tissues. In this study, out of more than 200 FOSC isolates obtained from symptomatic plants, the pathogenicity of 49 isolates was investigated. Among the pathogenic isolates (47 isolates) that were identified using a combination of species-specific primers and TEF-1α gene sequencing, about 85% were F. redolens and only about 10% were F. oxysporum f. sp. ciceris, these results show that F. redolens is the most prevalent soil-born pathogen that causes yellowing and wilting of chickpea in western Iran. This species has been reported in many chickpea-producing countries; however, the predominance of this pathogen has not been reported.</div>","PeriodicalId":8598,"journal":{"name":"Australasian Plant Pathology","volume":"55 4","pages":""},"PeriodicalIF":1.1,"publicationDate":"2026-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148008837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0