Marker- assisted backcross breeding to introgress bruchid [Callosobruchus maculatus (F.)] and mungbean yellow mosaic virus (MYMV) disease resistance in blackgram [Vigna mungo (L.) Hepper] variety MDU 1

IF 0.9 4区农林科学 Q4 PLANT SCIENCES

Australasian Plant Pathology Pub Date : 2025-01-20 DOI:10.1007/s13313-025-01018-5

Ragul Subramaniyan, Manivannan Narayana

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引用次数: 0

Abstract

Marker-assisted backcross (MABC) breeding technique was employed to introgress the Quantitative Trait Loci (QTL) regions for the bruchid resistance and Mungbean Yellow Mosaic Virus (MYMV) disease resistance from resistant donor TU 68 into the popular blackgram variety MDU 1. Bruchid pest and MYMV disease is the major pest and disease of blackgram that renders the crop growth and yield under severe epiphytotic conditions. Foreground selection was performed using six markers namely, CEDG 020, CEDG 067 in LG 5, CEDG 302, GMES 1248 in LG 8 and CEDG 180, CEDG 116 in LG 10 linked with bruchid pest and MYMV disease resistance. In addition, 40 Simple Sequence Repeats (SSR) markers found polymorphic among the MDU 1 × TU 68 population were used in background selection. Among the background introgressed lines (BILs), recurrent parent genome recovery ranged from 64.8 to 72.4%. Among these introgressed lines viz., BIL-95-5, BIL-95-3, BIL-95-1, BIL-135-5, BIL-33-2, BIL-20-3, BIL-20-1, BIL-135-2, and BIL-95-2 showed superior yield performance than the recurrent parent MDU 1. All the introgression lines exhibited significant resistance to bruchid and MYMV disease. Hence, these lines can be further advanced to large-scale trials to evaluate yield parameters, bruchid resistance, and MYMV disease resistance to release as a new improved variety.

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利用标记辅助回交育种，实现黑叶豆（Vigna mungo， L.）和绿豆黄花叶病毒（MYMV）抗性的引入。[Hepper]品种

采用标记辅助回交（MABC）育种技术，将抗病供体TU 68抗野田鼠病和绿豆黄花叶病毒（MYMV）病的数量性状位点（QTL）区域导入到流行的黑格品种MDU 1中。野鼠害和MYMV病是黑穗病的主要病虫害，在严重的附生条件下影响作物的生长和产量。利用LG 5的CEDG 020、CEDG 067、LG 8的CEDG 302、GMES 1248和LG 10的CEDG 180、CEDG 116与野鼠害虫和MYMV抗病相关的6个标记进行前景选择。此外，利用MDU 1 × TU 68群体中发现的40个多态性SSR标记进行背景选择。在背景渐渗系（BILs）中，亲本基因组恢复率为64.8% ~ 72.4%。其中，bill -95-5、bill -95-3、bill -95-1、bill -135-5、bill -33-2、bill -20-3、bill -20-1、bill -135-2和bill -95-2的产量表现优于循环亲本MDU 1。所有渗入系均表现出对野鼠病和MYMV病的显著抗性。因此，这些品系可以进一步推进到大规模试验，以评估产量参数、抗野鼠性和MYMV抗病释放作为新的改良品种。

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来源期刊

Australasian Plant Pathology 生物-植物科学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： Australasian Plant Pathology presents new and significant research in all facets of the field of plant pathology. Dedicated to a worldwide readership, the journal focuses on research in the Australasian region, including Australia, New Zealand and Papua New Guinea, as well as the Indian, Pacific regions. Australasian Plant Pathology is the official journal of the Australasian Plant Pathology Society.