Asian Pacific journal of tropical medicine最新文献_第10页

A staggering rise of HIV cases in Pakistan 巴基斯坦艾滋病病例的惊人增长

IF 3.1 4区医学

Asian Pacific journal of tropical medicine Pub Date : 2023-03-01 DOI: 10.4103/1995-7645.370153

Abdullah, R. Muhammad, M. Salman, A. Hussain, Amin Ullah, Muddasir Khan

引用次数: 0

Tapered optical fiber DNA biosensor for detecting Leptospira DNA 用于检测钩端螺旋体DNA的锥形光纤DNA生物传感器

IF 3.1 4区医学

Asian Pacific journal of tropical medicine Pub Date : 2023-03-01 DOI: 10.4103/1995-7645.372293

J. Lam, M. Yaacob, H. Chee

引用次数: 1

Mutations in Plasmodium knowlesi Kelch protein 13 and the dihydropteroate synthase gene in clinical samples 诺氏疟原虫Kelch蛋白13和二氢翼酸合成酶基因在临床样本中的突变

IF 3.1 4区医学

Asian Pacific journal of tropical medicine Pub Date : 2023-02-01 DOI: 10.4103/1995-7645.370146

A. Saif

{"title":"Mutations in Plasmodium knowlesi Kelch protein 13 and the dihydropteroate synthase gene in clinical samples","authors":"A. Saif","doi":"10.4103/1995-7645.370146","DOIUrl":"https://doi.org/10.4103/1995-7645.370146","url":null,"abstract":"Objective: To determine the genetic diversity, natural selection and mutations in Plasmodium (P.) knowlesi drug resistant molecular markers Kelch 13 and dhps gene in clinical samples of Malaysia. Methods: P. knowlesi full-length gene sequences Kelch 13 gene (PkK13) from 40 samples and dhps gene from 30 samples originating from Malaysian Borneo were retrieved from public databases. Genetic diversity, natural selection, and phylogenetic analysis of gene sequences were analysed using DNAsp v5.10 and MEGA v5.2. Results: Seventy-two single nucleotide polymorphic sites (SNPs) across the full-length PkK13 gene (63 synonymous substitutions and 9 non-synonymous substitutions) with nucleotide diversity of π~0.005 was observed. Analysis of the full-length Pkdhps gene revealed 73 SNPs and π~0.006 (44 synonymous substitutions and 29 non-synonymous substitutions). A high number of haplotypes (PkK13; H=37 and Pkdhps; H=29) with haplotype diversity of Hd ~0.99 were found in both genes, indicating population expansion. Nine mutant alleles were identified in PkK13 amino acid alignment of which, 7 (Asp3Glu, Lys50Gln, Lys53Glu, Ser123Thr, Ser127Pro, Ser149Thr and Ala169Thr) were within the Plasmodium specific domain, 2 (VaI372Ile and Lys424Asn) were in the BTB/POZ domain and no mutation was observed within the kelch propeller domain. The 29 non-synonymous mutations in the Pkdhps gene were novel and only presented in exon 1 and 2. Conclusions: Monitoring the mutations from clinical samples collected from all states of Malaysia along with clinical efficacy studies will be necessary to determine the drug resistance in P. knowlesi.","PeriodicalId":8559,"journal":{"name":"Asian Pacific journal of tropical medicine","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49578838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Molecular epidemiology of piliated pneumococcal isolates at a major tertiary hospital in the Klang Valley, Malaysia 马来西亚巴生谷一家大型三级医院中肺炎球菌毛状分离株的分子流行病学研究

IF 3.1 4区医学

Asian Pacific journal of tropical medicine Pub Date : 2023-02-01 DOI: 10.4103/1995-7645.370150

N. Dzaraly, M. Desa, AbdulRahman Muthanna, S. Masri, N. Taib, N. Baharin, N. Sulaiman, Nurul Rahman, N. Palanisamy, Z. Suhaili, Semiat Yusuff, N. Rahman, T. Soh, Fatimah Abdullah8

{"title":"Molecular epidemiology of piliated pneumococcal isolates at a major tertiary hospital in the Klang Valley, Malaysia","authors":"N. Dzaraly, M. Desa, AbdulRahman Muthanna, S. Masri, N. Taib, N. Baharin, N. Sulaiman, Nurul Rahman, N. Palanisamy, Z. Suhaili, Semiat Yusuff, N. Rahman, T. Soh, Fatimah Abdullah8","doi":"10.4103/1995-7645.370150","DOIUrl":"https://doi.org/10.4103/1995-7645.370150","url":null,"abstract":"Objective: To characterise a collection of pili-carrying and none pili-carrying pneumococcal isolates of clinical origin for serotypes, antibiotic resistance and genotype. Methods: In total, 42 clinical isolates were collected between October 2017 and December 2019. Those isolates were analysed for antimicrobial susceptibility, serotype distribution, detection of pneumococcal virulence and pilus genes. Multilocus sequence typing was performed only for piliated isolates, followed by phylogenetic analysis. Results: The common isolation sites among the pneumococcal isolates were tracheal aspirate (28.6%), blood (26.2%), and sputum (23.8%). Fifty percent isolates were resistant to erythromycin, tetracycline (50.0%) and trimethoprim-sulfamethoxazole (43.0%). The most frequent were serotypes 19F (28.6%), 6A/B (23.8%) and 19A (14.3%). Piliated isolates were detected in a small proportion (33.3%); 64.3% were multidrug-resistant. ST320 was the prevalent sequence type among the piliated isolates and genetically related to the Pneumococcal Molecular Epidemiology Network clones Taiwan19F-14 (CC271). In the phylogenetic analysis, some piliated isolates showed a close association having similar ST320, carrying serotype 19A and both pilus genes indicating their clonal spread. Conclusions: Pneumococcal lineages of piliated isolates have been globally disseminated and pili could have played a role in the spread of antibiotic resistant clones.","PeriodicalId":8559,"journal":{"name":"Asian Pacific journal of tropical medicine","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48255369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Treatment outcomes and adverse drug reactions among patients with drug–resistant tuberculosis receiving all-oral, long–term regimens: First record viewing report from Pakistan 接受所有口服、长期治疗的耐药结核病患者的治疗结果和药物不良反应：来自巴基斯坦的首次记录查看报告

IF 3.1 4区医学

Asian Pacific journal of tropical medicine Pub Date : 2023-02-01 DOI: 10.4103/1995-7645.370148

Hira M. Aslam, Asad Omar, R. Fatima, Usman Rasool, Aashifa Yaqoob, W. Ullah, Aamir Khan, Y. Khan, T. Mallhi

{"title":"Treatment outcomes and adverse drug reactions among patients with drug–resistant tuberculosis receiving all-oral, long–term regimens: First record viewing report from Pakistan","authors":"Hira M. Aslam, Asad Omar, R. Fatima, Usman Rasool, Aashifa Yaqoob, W. Ullah, Aamir Khan, Y. Khan, T. Mallhi","doi":"10.4103/1995-7645.370148","DOIUrl":"https://doi.org/10.4103/1995-7645.370148","url":null,"abstract":"Objective: To assess the effectiveness and adverse drug reactions of all-oral regimens for patients with multidrug-resistant tuberculosis. Methods: This retrospective study was conducted at 10 Programmatic Management of Drug Resistant Tuberculosis sites in Punjab province of Pakistan. Patients receiving treatment for drug resistant tuberculosis from July 2019 to December 2020 with at least interim result i.e. 6th month culture conversion or final outcomes (cured, complete, lost to follow-up, failure, death) available, were included in the study. Data was extracted from electronic data management system. For the reporting and management of adverse drug events, active tuberculosis drug safety monitoring and management was implemented across all sites. All the data was analyzed using SPSS version 22. Results: Out of 947 drug resistant tuberculosis patients included in this study, 579 (68%) of the patients had final outcomes available. Of these, 384 (67.9%) successfully completed their treatment. Out of 368 (32%) patients who had their interim results available, all had their 6th month culture negative. Combining new medications was thought to result in serious adverse outcomes such as QT prolongation. However, this study did not record any severe adverse events among patients. Conclusions: All-oral regimens formulation guided by overall treatment effectiveness resulted in treatment outcomes comparable to those obtained with traditional injectable treatment.","PeriodicalId":8559,"journal":{"name":"Asian Pacific journal of tropical medicine","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42436446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-term albendazole therapy for diffuse parenchymal neurocysticercosis in an immunocompetent patient: A case report 长期阿苯达唑治疗免疫功能正常患者弥漫性实质性神经囊虫病1例

IF 3.1 4区医学

Asian Pacific journal of tropical medicine Pub Date : 2023-02-01 DOI: 10.4103/1995-7645.368014

Paulus Sugianto, A. Machin, W. Islamiyah, Cindy Cecilia

引用次数: 0

Application of next-generation sequencing in thalassemia screening: A systematic review and meta-analysis 新一代测序在地中海贫血筛查中的应用:系统回顾和荟萃分析

IF 3.1 4区医学

Asian Pacific journal of tropical medicine Pub Date : 2023-02-01 DOI: 10.4103/1995-7645.370147

Xingyi Fang, Y. Gong, Yanlin Ma, Yuan-hua Huang

引用次数: 0

Neutralizing possibilities of whole virion and mRNA vaccine triggered antibodies of Wuhan strain of SARS-CoV-2 with receptor binding domains of spike proteins of Delta and Omicron strains 武汉株严重急性呼吸系统综合征冠状病毒2型抗体与德尔塔毒株和奥密克戎毒株刺突蛋白受体结合域的中和可能性

IF 3.1 4区医学

Asian Pacific journal of tropical medicine Pub Date : 2023-02-01 DOI: 10.4103/1995-7645.370152

Shilpa Barthwal, B. Angel, Annette Angel, V. Joshi, B. Shareef, N. Peer, Poorna Khaneja, A. Khan, Bhawna Sharma, Neha Singh, Ramesh Joshi, Komal Tomar, K. Yadav, Satendra Singh

引用次数: 1

Coagulation abnormalities and their relationship with bleeding manifestations in patients with dengue-A single center observational study 登革热患者凝血异常及其与出血表现的关系——单中心观察性研究

IF 3.1 4区医学

Asian Pacific journal of tropical medicine Pub Date : 2023-02-01 DOI: 10.4103/1995-7645.370149

Govind R. Patel, I. Thanvi, M. Nadeem, Rahul Kanwaria

{"title":"Coagulation abnormalities and their relationship with bleeding manifestations in patients with dengue-A single center observational study","authors":"Govind R. Patel, I. Thanvi, M. Nadeem, Rahul Kanwaria","doi":"10.4103/1995-7645.370149","DOIUrl":"https://doi.org/10.4103/1995-7645.370149","url":null,"abstract":"Objective: To evaluate coagulation abnormalities and their relationship with bleeding manifestations among patients with dengue. Methods: This observational study was conducted on 292 adult dengue patients who were admitted to a tertiary care hospital of Western India from July 2021 to June 2022. Coagulation tests including prothrombin time (PT), international normalized ratio (INR), activated partial thromboplastin time (aPTT), fibrinogen, and D-dimer were performed. Patients were monitored for bleeding manifestations. Results: Coagulation abnormalities were reported in 42.8% of the patients. Overall, prolonged aPTT was the most common coagulation abnormality (40.8%), followed by low fibrinogen (38.7%), raised D-dimer (31.2%), raised INR (26.0%) and prolonged PT (19.2%). Bleeding manifestations were present in 19.9% patients. PT, INR, aPTT and D-dimer levels were significantly higher (P<0.01) and fibrinogen level was significantly lower (P<0.001) in patients with bleeding compared to patients without bleeding. Patients with bleeding had a significantly higher rate of all coagulation abnormalities than patients without bleeding (P<0.01). Conclusions: Patients with bleeding showed a significantly higher frequency of coagulation abnormalities compared to patients without bleeding. Patients with dengue should be assessed for coagulation abnormalities.","PeriodicalId":8559,"journal":{"name":"Asian Pacific journal of tropical medicine","volume":null,"pages":null},"PeriodicalIF":3.1,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43837988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Seroprevalence of dengue virus among adults presenting with acute febrile illness at a tertiary care hospital in South India 南印度一家三级护理医院成人急性发热性疾病中登革热病毒的血清流行率

IF 3.1 4区医学

Asian Pacific journal of tropical medicine Pub Date : 2023-02-01 DOI: 10.4103/1995-7645.368021

Lavanya Mohanam, P. Shanmugam

引用次数: 0