Applied Entomology and Zoology最新文献_第6页

Discovery of the gall midge Schizomyia castanopsisae (Diptera: Cecidomyiidae) inducing inflorescence galls on Castanopsis sieboldii (Fagaceae) from Honshu, Japan and the possibility of its recent range expansion 日本本州壳斗科Castanopsiboldii（壳斗科）上发现了一种引起花序虫瘿的虫瘿蚊Schizomyiacastanopsisae（Diptera：Cecidomyidae）及其近期扩大范围的可能性

IF 1.3 4区农林科学

Applied Entomology and Zoology Pub Date : 2023-06-26 DOI: 10.1007/s13355-023-00834-9

Makoto Tokuda, Yoshifumi So, Nobuhiko Kotaka

{"title":"Discovery of the gall midge Schizomyia castanopsisae (Diptera: Cecidomyiidae) inducing inflorescence galls on Castanopsis sieboldii (Fagaceae) from Honshu, Japan and the possibility of its recent range expansion","authors":"Makoto Tokuda, Yoshifumi So, Nobuhiko Kotaka","doi":"10.1007/s13355-023-00834-9","DOIUrl":"10.1007/s13355-023-00834-9","url":null,"abstract":"<div>Outbreaks of herbivorous insects result in serious damage to forest trees. The gall midge Schizomyia castanopsisae (Diptera: Cecidomyiidae) induces inflorescence galls and causes severe loss of acorn production for Castanopsis sieboldii (Fagaceae). The outbreaks of gall midge have been occurring in these decades in the southern parts of the Izu Islands, Tokyo, Japan. In this study, we first found S. castanopsisae on Toshima Island, the Izu Islands, and in Izu Peninsula, Honshu. A genetic analysis showed that the genotype of the gall midge collected from the Izu Peninsula was identical to that from the Izu Islands, and only the haplotype was distributed in these areas. In contrast, the analysis of S. castanopsisae populations on the Nansei Islands of Japan found five haplotypes, including the one common to the Izu Peninsula and the Izu Islands. These results support the hypothesis that the gall midge populations occurring on the Izu Islands originate from the Nansei Islands, and the invaded populations have been expanding their range to the northern areas there. To monitor the distribution range and abundance of S. castanopsisae and its influences on the acorn production of C. sieboldii in Honshu are of urgent necessity.</div>","PeriodicalId":8551,"journal":{"name":"Applied Entomology and Zoology","volume":"58 4","pages":"315 - 322"},"PeriodicalIF":1.3,"publicationDate":"2023-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41654033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intra-species variation of ovipositor morphology and oviposition preference in Drosophila suzukii (Diptera: Drosophilidae) suzukii果蝇产卵器形态和产卵偏好的种内变异（直翅目：果蝇科）

IF 1.3 4区农林科学

Applied Entomology and Zoology Pub Date : 2023-06-23 DOI: 10.1007/s13355-023-00835-8

Junichi Akutsu, Takashi Matsuo

{"title":"Intra-species variation of ovipositor morphology and oviposition preference in Drosophila suzukii (Diptera: Drosophilidae)","authors":"Junichi Akutsu, Takashi Matsuo","doi":"10.1007/s13355-023-00835-8","DOIUrl":"10.1007/s13355-023-00835-8","url":null,"abstract":"<div>Females of Drosophila suzukii have an enlarged and sclerotized ovipositor (oviscapt, hypogynium), which is a key evolutionary trait that enabled D. suzukii to lay eggs through the skin of ripening fruits. We investigated the intra-species variation of D. suzukii in ovipositor morphology and oviposition preference for substrate hardness among 27 strains recently established from local populations in Japan. A significant difference between strains was observed in the length of the ovipositor as well as in the number of bristles on it. Further analysis using representative strains revealed that the ovipositor size variation among strains was independent of the body size variation, suggesting that the proportion between the ovipositor and other body parts was different among the strains. In the two-choice oviposition assay using 2% and 7% agar substrates, four strains preferred the soft substrates, and one strain preferred the hard substrates. Competitive cross-modal assay between substrate hardness and surface curvature revealed that the preference for curvature overrode the preference for hardness in all the tested strains.</div>","PeriodicalId":8551,"journal":{"name":"Applied Entomology and Zoology","volume":"58 4","pages":"323 - 328"},"PeriodicalIF":1.3,"publicationDate":"2023-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13355-023-00835-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46513225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Towards automatic monitoring of insect pests using IoT camera-equipped pheromone traps: a case study for Spodoptera litura (Lepidoptera: Noctuidae) 利用物联网摄像头信息素捕捉器实现害虫的自动监测——以斜纹夜蛾（鳞翅目：夜蛾科）为例

IF 1.3 4区农林科学

Applied Entomology and Zoology Pub Date : 2023-06-18 DOI: 10.1007/s13355-023-00830-z

Satoshi Kawakita, Tatsuya Sato

{"title":"Towards automatic monitoring of insect pests using IoT camera-equipped pheromone traps: a case study for Spodoptera litura (Lepidoptera: Noctuidae)","authors":"Satoshi Kawakita, Tatsuya Sato","doi":"10.1007/s13355-023-00830-z","DOIUrl":"10.1007/s13355-023-00830-z","url":null,"abstract":"<div>To efficiently monitor insect pests, we developed an electronic device that incorporates a pheromone trap and an Internet of things (IoT) camera (E-trap) that can semi-automatically check the daily number of captured insects. Images captured by the IoT camera were transmitted over a 4G wireless network, and the number of insects captured by the trap was visually confirmed from the images. Our E-trap and two types of conventional (funnel-type and dry-type) traps were placed in two soybean fields in Japan in 2021. The trends of Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae) caught daily by the E-trap and those observed at five-day intervals by the conventional traps were compared. Although the general trends in the occurrence of S. litura were similar to the data obtained among the different trapping methods, the data obtained daily by the E-trap showed a more detailed change in trap catches. The coefficient of determination between the number of S. litura visually estimated from the images and the actual number of S. litura caught was greater than 0.95. This demonstrates the practicality of the proposed E-trap, which minimizes the need for monitoring and maintaining the insect traps.</div>","PeriodicalId":8551,"journal":{"name":"Applied Entomology and Zoology","volume":"58 3","pages":"265 - 272"},"PeriodicalIF":1.3,"publicationDate":"2023-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45553622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Induction of leaf curling in cassava plants by the cassava mealybug Phenacoccus manihoti (Hemiptera: Pseudococcidae) 木薯粉蚧Phenacoccus manihoti诱导木薯植株卷曲叶片（半翅目：假球虫科）

IF 1.3 4区农林科学

Applied Entomology and Zoology Pub Date : 2023-06-14 DOI: 10.1007/s13355-023-00832-x

Shun-ichiro Takano, Yoshinori Utsumi, Atsushi Nagano, Satoshi Takahashi, Akihiro Ezoe, Motoaki Seki, Thi Xuyen Le, Keiji Takasu

{"title":"Induction of leaf curling in cassava plants by the cassava mealybug Phenacoccus manihoti (Hemiptera: Pseudococcidae)","authors":"Shun-ichiro Takano, Yoshinori Utsumi, Atsushi Nagano, Satoshi Takahashi, Akihiro Ezoe, Motoaki Seki, Thi Xuyen Le, Keiji Takasu","doi":"10.1007/s13355-023-00832-x","DOIUrl":"10.1007/s13355-023-00832-x","url":null,"abstract":"<div>Certain phytophagous insects can induce leaf curling in their host plants that may provide protected and nutrient-rich habitats. However, the mechanisms of this induction remain poorly understood. The cassava mealybug, Phenacoccus manihoti Matile-Ferrero (Hemiptera: Pseudococcidae), is a serious pest of the cassava and causes leaf curling. To reveal the mechanisms of leaf-curl induction, we first inoculated varying numbers of mealybugs in different locations, namely, the apical meristem and the stem, on cassava seedlings. Second, we performed transcriptome analysis using the total RNA extracted from leaves. The results showed that a single insect was able to induce leaf curling, but the intensity and frequency of the leaf curling were positively correlated with the number of insects. Furthermore, the leaf curling occurred when the mealybugs fed on or close to the apical meristem but not when they fed on the stem. Transcriptome analysis identified a total of 3,931 differentially expressed genes (DEGs) from intact plants and the plants inoculated with mealybugs at different time points. GO analysis of the biological processes revealed that the DEGs contained a series of factors for leaf development of the adaxial–abaxial axis, and auxin biosynthesis and polarity. This suggests that alterations in these functions may cause leaf curling.</div>","PeriodicalId":8551,"journal":{"name":"Applied Entomology and Zoology","volume":"58 3","pages":"279 - 290"},"PeriodicalIF":1.3,"publicationDate":"2023-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43178608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DIPA-CRISPR gene editing in the yellow fever mosquito Aedes aegypti (Diptera: Culicidae) DIPA-CRISPR基因编辑在黄热病蚊子埃及伊蚊中的应用(双翅目:库蚊科)

IF 1.3 4区农林科学

Applied Entomology and Zoology Pub Date : 2023-06-09 DOI: 10.1007/s13355-023-00831-y

Yu Shirai, Momoyo Takahashi, Manabu Ote, Hirotaka Kanuka, Takaaki Daimon

{"title":"DIPA-CRISPR gene editing in the yellow fever mosquito Aedes aegypti (Diptera: Culicidae)","authors":"Yu Shirai, Momoyo Takahashi, Manabu Ote, Hirotaka Kanuka, Takaaki Daimon","doi":"10.1007/s13355-023-00831-y","DOIUrl":"10.1007/s13355-023-00831-y","url":null,"abstract":"<div>Current methods for gene editing in insects rely on embryonic microinjection, which can be challenging for non-specialist laboratories. Recently, an alternative method known as “direct parental” CRISPR (DIPA-CRISPR) was developed. This method involves injecting commercial Cas9 protein and single-guide RNA into adult females, which can efficiently introduce mutations into developing oocytes. However, its versatility has not been fully explored, particularly in insects that have the most derived, polytrophic meroistic ovaries. In this study, we successfully applied DIPA-CRISPR to the yellow fever mosquito Aedes aegypti, which has polytrophic meroistic ovaries. Following adult injection of Cas9 ribonucleoproteins (Cas9 RNPs) targeting the kynurenine 3-monooxygenase gene, we recovered gene-edited G0 individuals. Injection at 24 h after blood-feeding resulted in the highest gene editing efficiency (3.5%), confirming that a key parameter of DIPA-CRISPR is the stage in which the adult females are injected. Together with our previous study, we demonstrated that DIPA-CRISPR is applicable to all three types of insect ovaries (i.e., panoistic, telotrophic, and polytrophic), which indicates that DIPA-CRISPR is a generalizable approach for insect gene editing.</div>","PeriodicalId":8551,"journal":{"name":"Applied Entomology and Zoology","volume":"58 3","pages":"273 - 278"},"PeriodicalIF":1.3,"publicationDate":"2023-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50016729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Sex and strain-specific spectral attraction of Tribolium castaneum (Coleoptera: Tenebrionidae): behavioral studies 黄颡鱼纲(鞘翅目:拟步甲科)的性别和品系特异性光谱吸引:行为研究

IF 1.3 4区农林科学

Applied Entomology and Zoology Pub Date : 2023-06-07 DOI: 10.1007/s13355-023-00829-6

Sota Sone, Takahisa Miyatake

引用次数: 0

Characterization of the atypical Meloidogyne arenaria (Tylenchina: Meloidogynidae) in Japan 日本非典型竞技场根结线虫的特征（Tylenchina:根结线虫科）

IF 1.3 4区农林科学

Applied Entomology and Zoology Pub Date : 2023-05-11 DOI: 10.1007/s13355-023-00828-7

Gaku Murata, Toshihisa Yashiro, Taketo Uehara, Kenta Uesugi, Hideaki Iwahori, Takashi Narabu

{"title":"Characterization of the atypical Meloidogyne arenaria (Tylenchina: Meloidogynidae) in Japan","authors":"Gaku Murata, Toshihisa Yashiro, Taketo Uehara, Kenta Uesugi, Hideaki Iwahori, Takashi Narabu","doi":"10.1007/s13355-023-00828-7","DOIUrl":"10.1007/s13355-023-00828-7","url":null,"abstract":"<div>Genus Meloidogyne parasitizes and causes galling on host roots with heavy yield losses. Meloidogyne arenaria (Neal 1889) Chitwood 1949, one of the major species, is found in Japan and can be divided into two genotypes: M. arenaria A2-J and A2-O. Whereas the latter is typical M. arenaria, the former is empirically considered as atypical morphologically and genetically, but its detailed characteristics and phylogenetic relationships have not been described. First, we examined the perineal pattern, isozyme pattern, and PCR products with the C2F3/1108 primer set of M. arenaria A2-J and confirmed that all the populations of M. arenaria A2-J used in this study showed M. javanica and/or M. incognita -like perineal patterns, the A2 pattern in esterase isozyme, and approx. 1.7 kb amplicon. We then carried out a phylogenetic analysis based on the sequences of a partial region of COXII-IrDNA in mitochondrial DNA to reveal the relationships between M. arenaria A2-J and related species using corresponding sequences of Meloidogyne accessions deposited in GenBank. Moreover, host suitability of representative Solanaceae plants including Solanum torvum was evaluated and compared to those of major species of Meloidogyne collected from Japan.\u0000</div>","PeriodicalId":8551,"journal":{"name":"Applied Entomology and Zoology","volume":"58 3","pages":"245 - 255"},"PeriodicalIF":1.3,"publicationDate":"2023-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13355-023-00828-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42139952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characteristics of the takeout protein ce-0330 in the silkworm, Bombyx mori (Lepidoptera: Bombycidae) 家蚕外卖蛋白ce-0330的特征(鳞翅目:家蚕科)

IF 1.3 4区农林科学

Applied Entomology and Zoology Pub Date : 2023-05-02 DOI: 10.1007/s13355-023-00827-8

Takahiro Shiotsuki, Rintaro Suzuki, Wataru Tsuchiya, Toshimasa Yamazaki, Masaru Shimomura, Takuya Tsubota, Takayo Nakakura, Shuhei Henmi

{"title":"Characteristics of the takeout protein ce-0330 in the silkworm, Bombyx mori (Lepidoptera: Bombycidae)","authors":"Takahiro Shiotsuki, Rintaro Suzuki, Wataru Tsuchiya, Toshimasa Yamazaki, Masaru Shimomura, Takuya Tsubota, Takayo Nakakura, Shuhei Henmi","doi":"10.1007/s13355-023-00827-8","DOIUrl":"10.1007/s13355-023-00827-8","url":null,"abstract":"<div>Insect juvenile hormone (JH)-binding protein (JHBP) transports JH in the hemolymph. The JHBP gene is a member of the takeout gene superfamily, which has more than 10 genes belonging to each species and that play different physiological roles. To characterize these genes, expression analysis of the takeout genes in silkworms was conducted using real-time quantitative RT-PCR. The expression profiles varied for each gene. For example, some genes, including Bm_ce-0330, were expressed in the adult antennae. The functions of takeout proteins in lepidopterans, except for JHBP, remain unknown. Among the Bombyx takeout proteins, Bm_ce-0330 shares a high sequence homology with Epiphyas postvittana takeout 1, the 3-D structure of which has been known recently. Bm_ce-0330 was predicted to bind to the odorant expressed in the adult antennae, with a low ligand selectivity. We collectively propose a strategy to predict the unknown ligands and physiological role of takeout proteins from gene expression patterns and actual and/or computer-associated modeling of 3-D molecular structures.</div>","PeriodicalId":8551,"journal":{"name":"Applied Entomology and Zoology","volume":"58 3","pages":"237 - 244"},"PeriodicalIF":1.3,"publicationDate":"2023-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43389748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mating behaviors and multiple mating in the firebrat, Thermobia domestica (Zygentoma: Lepismatidae) 火鼠Thermobia domestica的交配行为和多次交配（Zyngentoma:鳞翅目）

IF 1.3 4区农林科学

Applied Entomology and Zoology Pub Date : 2023-04-19 DOI: 10.1007/s13355-023-00826-9

Kei Inada, Toshinori Minemura, Takahiro Ohde, Takaaki Daimon

{"title":"Mating behaviors and multiple mating in the firebrat, Thermobia domestica (Zygentoma: Lepismatidae)","authors":"Kei Inada, Toshinori Minemura, Takahiro Ohde, Takaaki Daimon","doi":"10.1007/s13355-023-00826-9","DOIUrl":"10.1007/s13355-023-00826-9","url":null,"abstract":"<div>While most terrestrial animals undergo internal fertilization, some insects use external transfer of sperm during mating. Among these are insects belonging to the orders Zygentoma (silverfish and firebrats) and Odonata (dragonflies and damselflies), which employ external sperm transfer. Although the mating processes of the latter group are well understood, those of the former group have remained obscure. Here we present a detailed description of mating in the firebrat, Thermobia domestica (Packard) (Zygentoma: Lepismatidae), involving external sperm transfer. We found that mating behaviors of the firebrat are complex and largely modular, with four distinct recognizable elements: facing, turning around, back and forth, and ejaculation and straddling. Interestingly, males do not guard or show further interest in females after depositing the spermatheca on the ground. To investigate whether firebrats engage in multiple matings, we performed male-informative crossing experiments by using a gene-edited mutant line. Our results revealed that when females were paired with two males of different genotypes, 48% of the recovered F1 clutches (24 out of 50) were derived from multiple matings, providing strong evidence for frequent multiple mating in the firebrat.</div>","PeriodicalId":8551,"journal":{"name":"Applied Entomology and Zoology","volume":"58 3","pages":"297 - 302"},"PeriodicalIF":1.3,"publicationDate":"2023-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45769278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of quorum sensing inducers and inhibitors on male-killing Wolbachia, the endosymbiont of the adzuki bean borer, Ostrinia scapulalis (Lepidoptera: Crambidae) 群体感应诱导剂和抑制剂对小豆螟内共生体沃尔巴克氏体灭雄的影响

IF 1.3 4区农林科学

Applied Entomology and Zoology Pub Date : 2023-04-15 DOI: 10.1007/s13355-023-00825-w

Ardhiani Kurnia Hidayanti, Takafumi N. Sugimoto, Achmad Gazali, Yohsuke Tagami

{"title":"Effect of quorum sensing inducers and inhibitors on male-killing Wolbachia, the endosymbiont of the adzuki bean borer, Ostrinia scapulalis (Lepidoptera: Crambidae)","authors":"Ardhiani Kurnia Hidayanti, Takafumi N. Sugimoto, Achmad Gazali, Yohsuke Tagami","doi":"10.1007/s13355-023-00825-w","DOIUrl":"10.1007/s13355-023-00825-w","url":null,"abstract":"<div>Ostrinia scapulalis (Walker) (Lepidoptera: Crambidae), the adzuki bean borer, is a serious pest in adzuki bean plants and other dicotyledons. Wolbachia, the insect symbiont that infects O. scapulalis, causes male-killing during the embryonic and larval stages in the insect host. Previous studies have reported an association between Wolbachia density dynamics and the sex phenotype of offspring. In this study, we treated O. scapulalis at the adult and larval stages using 3O-C12-HSL as a quorum sensing (QS) inducer and 4-phenylbutanol as a QS inhibitor. QS inducer treatment in the adult stage increased the density of Wolbachia in O. scapulalis, and there was a strong positive correlation between Wolbachia density and female proportion in the adult stage after QS inhibitor treatment. QS inducer and inhibitor treatments in the larval stage stabilize Wolbachia density in O. scapulalis.</div>","PeriodicalId":8551,"journal":{"name":"Applied Entomology and Zoology","volume":"58 3","pages":"229 - 236"},"PeriodicalIF":1.3,"publicationDate":"2023-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13355-023-00825-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47140342","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0