Eiriki Sunamura, Shigeaki Tamura, Hisatomo Taki, Grzegorz Buczkowski, Etsuko Shoda-Kagaya
{"title":"Effects of neonicotinoid insecticide trunk injections on non-target arboreal ants, potential biological control agents for invasive longhorn beetle Aromia bungii on cherry trees","authors":"Eiriki Sunamura, Shigeaki Tamura, Hisatomo Taki, Grzegorz Buczkowski, Etsuko Shoda-Kagaya","doi":"10.1007/s13355-023-00844-7","DOIUrl":"10.1007/s13355-023-00844-7","url":null,"abstract":"<div><p>Trunk injection is a relatively new, environmentally friendly method to apply insecticides to trees which does not cause insecticide drift and environmental runoff. However, little is known about the effect of insecticide trunk injection on non-target arboreal ants (Hymenoptera: Formicidae) which can act as biological control agents of target tree pests. This study evaluated the effect of trunk injections on arboreal ants found on cherry trees treated with neonicotinoids (dinotefuran and thiamethoxam) for controlling the invasive longhorn beetle <i>Aromia bungii</i> (Faldermann) (Coleoptera: Cerambycidae). Arboreal ants represented by <i>Crematogaster matsumurai</i> Forel can prey on <i>A. bungii</i> eggs. Results of visual sampling 1 and 3 months after injections showed that injections did not reduce the number of ant species occurring on tree trunks. Additionally, injections did not eliminate 3 of 4 most abundant species on tree trunks or extrafloral nectaries including <i>C. matsumurai</i>. However, a decline of <i>Lasius japonicus</i> Santschi was observed on injected trees. Our preliminary short-term survey suggests the possibility that chemical control by trunk injection and biological control by arboreal ants are compatible in <i>A. bungii</i> management. However, further research is needed to clarify the mechanism of <i>L. japonicus</i> decline and long-term consequences of trunk injection on arboreal ant composition.</p></div>","PeriodicalId":8551,"journal":{"name":"Applied Entomology and Zoology","volume":"58 4","pages":"401 - 407"},"PeriodicalIF":1.3,"publicationDate":"2023-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13355-023-00844-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"50017146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Laboratory experiments of Stigmatomyces majewskii (Laboulbeniales: Laboulbeniaceae) infection on Drosophila suzukii (Diptera: Drosophilidae)","authors":"Izumi Yamazaki, Moe Onuma, Tomohiko Ri, Izumi Okane, Natsumi Kanzaki, Yousuke Degawa, Kyoichi Sawamura","doi":"10.1007/s13355-023-00843-8","DOIUrl":"10.1007/s13355-023-00843-8","url":null,"abstract":"<div><p>Originally endemic to Asia, <i>Drosophila suzukii</i> (Matsumura, 1931) is a serious agricultural pest that is spreading rapidly throughout the world. To suppress the growing <i>D. suzukii</i> populations, several pest management programs have been implemented. Researchers have searched for natural enemies, such as predators, parasites, parasitoids, and pathogens. We discovered an obligate ectobiont fungus, <i>Stigmatomyces majewskii</i> H. L. Dainat, Manier & Balazuc, 1974, which is associated with <i>D. suzukii</i>. Our laboratory experiments revealed that <i>S. majewskii</i> infection was successfully transmitted through self-grooming and sexual contact during the courtship behavior of <i>D. suzukii,</i> although non-sexual contact through other behaviors may also be involved. Infected flies lived long enough for <i>S. majewskii</i> to mature on the host. Other fitness traits, such as fecundity, are necessary to evaluate the effect of <i>S. majewskii</i> on <i>D. suzukii</i>.</p></div>","PeriodicalId":8551,"journal":{"name":"Applied Entomology and Zoology","volume":"58 4","pages":"379 - 385"},"PeriodicalIF":1.3,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43257011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Methylobacterium sp. isolated from the midgut of Anopheles stephensi inhibits egg maturation in host ovary","authors":"Jun Hakozaki, Yusuke Nonogaki, Taishi Tanabe, Keita Nishiyama, Kazuhiko Nakayama, Asako Haraguchi, Sakure Nakamura, Kodai Kusakisako, Hiromi Ikadai","doi":"10.1007/s13355-023-00842-9","DOIUrl":"10.1007/s13355-023-00842-9","url":null,"abstract":"<div><p>The gut bacteria of insects are associated with digestion, nutrition, and reproduction. The midgut bacteria of <i>Anopheles</i> mosquitoes have been examined predominantly for their effects on the transmission of <i>Plasmodium</i> parasites. However, the effects of midgut bacteria on <i>Anopheles</i> mosquitoes have not been studied. In this study, we investigated the effects of midgut bacteria on adult female <i>An. stephensi</i> mosquitoes<i>.</i> These mosquitoes were fed antibiotics mixed with 10% sucrose for 2 weeks to alter the composition of their midgut bacteria. The results showed that the mosquitoes fed with streptomycin (ST) or kanamycin (KM) showed inhibited egg maturation 2 days after blood feeding. <i>Methylobacterium</i> sp. was the predominant bacterium in the midgut of mosquitoes fed KM or ST for 2 weeks (ST/2 weeks). Mosquitoes that were fed suspensions of <i>Methylobacterium</i> sp. isolated from the midgut of ST/2 weeks after blood feeding for 3 days showed inhibited egg maturation. Furthermore, egg maturation was inhibited in mosquitoes that were fed supernatant and precipitate obtained by boiling and centrifuging <i>Methylobacterium</i> sp. suspension. Thus, this study demonstrated that <i>Methylobacterium</i> sp. isolated from the midgut of <i>An. stephensi</i> mosquitoes after blood feeding inhibited egg maturation.</p></div>","PeriodicalId":8551,"journal":{"name":"Applied Entomology and Zoology","volume":"58 4","pages":"369 - 377"},"PeriodicalIF":1.3,"publicationDate":"2023-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41934058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Paratthakorn Ongsirimongkol, Patchara Sirasoonthorn, Katsumi Kamiya, Chieka Minakuchi, Ken Miura
{"title":"Cuticular protein genes are involved with insecticide resistance mechanism in red flour beetles, Tribolium castaneum (Coleoptera: Tenebrionidae)","authors":"Paratthakorn Ongsirimongkol, Patchara Sirasoonthorn, Katsumi Kamiya, Chieka Minakuchi, Ken Miura","doi":"10.1007/s13355-023-00841-w","DOIUrl":"10.1007/s13355-023-00841-w","url":null,"abstract":"<div><p>The insect integument, which consists mainly of chitin microfibrils and cuticular proteins, is an extracellular matrix that covers the entire external and some internal surfaces of the insect body and serves as a primary barrier against several environmental stresses. Using RNAi-mediated gene knockdown procedure, we performed functional analyses of three adult-specific cuticular protein genes, <i>CPR4</i>, <i>CPR18</i>, and <i>CPR27</i>, regarding their involvement in repelling chemical insecticides in the red flour beetles, <i>Tribolium castaneum</i> (Herbst) (Coleoptera: Tenebrionidae). The depletion of these CPRs produced adult phenotypes with malformed (wrinkled/dimpled) elytra as well as thinner and disorganized cuticle of abdominal sternite. After knocking down the <i>CPR</i> genes, the beetles were exposed to six types of neonicotinoid-class insecticides, and the short-term neurotoxic effects were evaluated. The knockdown of any of <i>CPR</i> genes, either singly or in combination, increased the beetles’ susceptibility to neonicotinoids compared to negative control beetles. The results suggested that <i>CPR4</i>, <i>CPR18</i>, and <i>CPR27</i> have an indispensable role in organizing the insect integument structure that can regulate the penetration rate of xenobiotics such as neonicotinoid insecticides, presumably by helping built proper structural features of cuticular layer.</p></div>","PeriodicalId":8551,"journal":{"name":"Applied Entomology and Zoology","volume":"58 4","pages":"357 - 368"},"PeriodicalIF":1.3,"publicationDate":"2023-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41446755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Qian Zhao, Dong-kang Zhang, Ling-ling Jiang, Jie Guo, Hong-ying Hu
{"title":"Globular galls on Haloxylon (Centrospermae: Chenopodiaceae) induced by gall midges, and the corresponding parasitoids in China","authors":"Qian Zhao, Dong-kang Zhang, Ling-ling Jiang, Jie Guo, Hong-ying Hu","doi":"10.1007/s13355-023-00840-x","DOIUrl":"10.1007/s13355-023-00840-x","url":null,"abstract":"<div><p>The concealed gall inducers represent a significant challenge for both study and control within the realm of pest management. Gaining an understanding of the natural enemies that influence gall inducer populations is essential for the development of effective integrated pest control strategies. <i>Haloxylon</i> spp. are dominant xerophyte species that possess substantial ecological importance due to their roles in shelter provision and sand fixation. This study aimed to determine the main gall inducers that induce globular galls on both <i>H. ammodendron</i> and <i>H. persicum</i> and the parasitoid complex associated with them. Totally, two species of gall inducers and four species of parasitoids were obtained from the globular galls. <i>Suaediola</i> sp. was recorded as the predominant gall inducer, exhibiting biannual generational turnover. Both <i>Torymus</i> sp. and <i>Mesopolobus quadrimaculatus</i> were primary solitary koinobiont ectoparasitoids of gall midges, while only <i>Platygaster</i> sp. was endoparasitoid. <i>Torymus</i> sp. established dominance among the parasitoids, demonstrating a predilection for attacking first instar larvae. We also found that temperature variations significantly influenced the emergence and lifespan of the insects recorded from the galls, with a consistent trend of shortened wasp lifespan correlating with increasing temperature. According to these results, we conclude <i>Torymus</i> sp. could have an important potential to control gall inducers within closed galls and, therefore, should be considered for inclusion in the integrated pest management programs of <i>Haloxylon</i> forests in China.</p></div>","PeriodicalId":8551,"journal":{"name":"Applied Entomology and Zoology","volume":"58 4","pages":"345 - 356"},"PeriodicalIF":1.3,"publicationDate":"2023-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13355-023-00840-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47532749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Structural and functional studies of pattern recognition receptors βGRP1 and βGRP2 in Sogatella furcifera","authors":"ChunLi Luo, SiQi Chen, FeiYan Gou, DaoWei Zhang, Jing Chen","doi":"10.1007/s13355-023-00833-w","DOIUrl":"10.1007/s13355-023-00833-w","url":null,"abstract":"<div><p>Beta-1,3-glucan recognition protein (βGRP) is an important pattern recognition receptor, which induces an immune response by recognizing and binding the pathogenic bacteria. In this study, we identified two <i>βGRP</i> genes in <i>S. furcifera</i>, <i>βGRP1</i> and <i>βGRP2</i>. Both βGRP1 and βGRP2 proteins have a glycosyl hydrolases family 16 (GH16) domain and a concanavalin A-like lectin/glucanase domain near the C-terminal. Quantitative polymerase chain reaction (qRT-PCR) analysis showed that the transcript levels of <i>βGRP1</i> and <i>βGRP2</i> in the fat body and gut were higher than those in other tissues. Furthermore, both were upregulated in response to challenges with <i>Escherichia coli</i> and <i>Staphylococcus aureus</i>. Recombinant βGRP1 and βGRP2 had a strong affinity for <i>E</i><i>. coli</i> and <i>S. aureus</i> and caused bacteria to agglutinate. However, the results of the CCK-8 and bacteriostatic zone methods showed that recombinant βGRP1 and βGRP2 inhibited <i>S. aureus</i> but did not inhibit the growth of <i>E. coli.</i> Moreover, the silencing of <i>βGRP1</i> or <i>βGRP2</i> using dsRNA significantly downregulated the expression of the Toll pathway gene <i>Dorsal</i> after <i>S. aureus</i> challenge, while it did not affect the Imd pathway gene <i>Relish</i>.</p></div>","PeriodicalId":8551,"journal":{"name":"Applied Entomology and Zoology","volume":"58 4","pages":"303 - 313"},"PeriodicalIF":1.3,"publicationDate":"2023-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44392020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shabnam Zamani Fard, Seyed Ali Hemmati, Parviz Shishehbor
{"title":"Biological and population growth traits of Spodoptera littoralis (Lepidoptera: Noctuidae) on various mung bean (Vigna radiata) varieties","authors":"Shabnam Zamani Fard, Seyed Ali Hemmati, Parviz Shishehbor","doi":"10.1007/s13355-023-00839-4","DOIUrl":"10.1007/s13355-023-00839-4","url":null,"abstract":"<div><p><i>Spodoptera littoralis</i> (Boisd) is globally known as an important polyphagous pest that causes considerable damage to agricultural crops in the world. In this study, the effects of different mung bean (<i>Vigna radiata</i> L.) varieties, including Parto, Simite1, Simite2, VC6371, VC3960, VC6173A, and VC6368 were investigated on the life history variables, biological characteristics, and population growth parameters of <i>S. littoralis</i>. Our purpose was to identify potential tolerance characteristics of mung bean varieties against <i>S. littoralis</i>. Our findings showed that various mung bean varieties significantly impact the life history and demographical parameters of <i>S. littoralis</i>. The results revealed that the longest and shortest developmental time across all immature stages were recorded on Simite2 (42.52 days) and VC6371 (37.40 days) varieties, respectively. The lowest fecundity values were observed on the Simite2 host (89.27 eggs), while the highest was on the VC6173A host (626.59 eggs). The highest and lowest values of net reproductive rate (<i>R</i><sub><i>0</i></sub>) were 233.17 and 25.36 offspring on VC6173A and Simite2 varieties, respectively. Furthermore, <i>S. littoralis</i> showed the highest intrinsic rate of increase (<i>r</i>) when reared on the VC6371 variety (0.99 day<sup>−1</sup>), while the lowest value was on the Simite2 (0.11 day<sup>−1</sup>) variety. Moreover, <i>S. littoralis</i> fed on the Simite2 (46.47 days) and VC6371 varieties (42.27 days) achieved the longest and shortest generation time (<i>T</i>). The cluster analysis results indicated that VC6173A was a susceptible variety, while the Simite2 variety revealed tolerance behaviors against <i>S. littoralis</i> and can be considered for IPM strategies.</p></div>","PeriodicalId":8551,"journal":{"name":"Applied Entomology and Zoology","volume":"58 4","pages":"335 - 344"},"PeriodicalIF":1.3,"publicationDate":"2023-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44397608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Structural remodeling of midgut symbiotic organ and altered food flow upon metamorphosis of the stinkbug Plautia stali","authors":"Sayumi Oishi, Minoru Moriyama, Takema Fukatsu","doi":"10.1007/s13355-023-00838-5","DOIUrl":"10.1007/s13355-023-00838-5","url":null,"abstract":"<div><p>Animals possess a mouth for feeding, an anus for defecation, and a gut structurally and functionally connecting them for digestion and absorption. In some stinkbugs, strikingly, the mouth and the anus are functionally disconnected in the middle. The constricted region blocks food flow and selectively allows passing of a specific bacterial symbiont to maintain the posterior midgut region as an exclusive space for symbiosis. In the stinkbug <i>Plautia stali</i>, the constricted region was reported to open during the last nymphal instar, thereby restoring the normal gut passage in adult insects. Here, we experimentally investigated how the structural gut reorganization upon metamorphosis of <i>P. stali</i> affects the processing of liquid food and solid food using blue coloring and fluorescent microbeads. In nymphal insects, both coloring and microbeads were blocked at the constricted region, and only coloring was excreted to the hindgut via Malpighian tubules. In adult insects, both coloring and microbeads passed through the constricted region and excreted to the hindgut. These results suggested that nymphal <i>P. stali</i> can utilize liquid food only whereas adult <i>P. stali</i> can live on both liquid food and solid suspension food, which may be relevant to nutritional and reproductive differences between nymphal and adult stinkbugs.</p></div>","PeriodicalId":8551,"journal":{"name":"Applied Entomology and Zoology","volume":"58 4","pages":"393 - 399"},"PeriodicalIF":1.3,"publicationDate":"2023-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48386089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"First definitive host record of Liopteridae revealed by biological observations and molecular biological assays: Paramblynotus koreanus (Hymenoptera: Liopteridae) is a parasitoid of Carcilia strigicollis (Coleoptera: Curculionidae)","authors":"Hiroshi Kodama, Tatsuya Ide","doi":"10.1007/s13355-023-00837-6","DOIUrl":"10.1007/s13355-023-00837-6","url":null,"abstract":"<div><p>Biological observations of <i>Paramblynotus koreanus</i> Choi and Suh (Hymenoptera: Cynipoidea: Liopteridae) occurred on <i>Quercus serrata</i> Thunb. Trees suggested that their host was <i>Carcilia strigicollis</i> Roelofs (Coleoptera: Curculionidae). The species-specific loop-mediated isothermal amplification (LAMP) assay designed for <i>C</i>. <i>strigicollis</i> successfully detected <i>C</i>. <i>strigicollis</i> DNA in the residue of <i>P</i>. <i>koreanus</i> pupal chamber, which demonstrated that this species is a host of <i>P</i>. <i>koreanus</i>. This is the first definitive host record for the Liopteridae.</p></div>","PeriodicalId":8551,"journal":{"name":"Applied Entomology and Zoology","volume":"58 4","pages":"387 - 391"},"PeriodicalIF":1.3,"publicationDate":"2023-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13355-023-00837-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47038233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Pheromone-baited cone traps are efficient for catching the bean webworm, Pleuroptya ruralis (Lepidoptera: Crambidae)","authors":"Kazuki Shibuya, Nobuyuki Endo, Hiroaki Takeuchi","doi":"10.1007/s13355-023-00836-7","DOIUrl":"10.1007/s13355-023-00836-7","url":null,"abstract":"<div><p>The bean webworm, <i>Pleuroptya ruralis</i> (Scopoli) (Lepidoptera: Crambidae), is a serious soybean pest in northern Japan. Monitoring <i>P. ruralis</i> has been carried out by beating the soybean canopy and counting the number of displaced adults; however, this method is laborious and time-consuming. To develop attractant lures for monitoring traps, the sex pheromone of <i>P. ruralis</i> has already been identified; however, pheromone-baited traps have failed to catch a sufficient number of moths. Previous trials solely utilized delta traps; cone traps, which are demonstrated to be effective for trapping some crambid species, have not yet been evaluated in <i>P. ruralis</i>. In this study, we compared the capture efficiency of pheromone-baited cone traps with those of pheromone-baited delta traps along with the beating method. The number of <i>P. ruralis</i> caught by the cone traps was significantly and 25 times larger than those caught by the delta traps. This result indicates that the trap design dramatically affects the capture efficiency of <i>P. ruralis</i>, and the cone trap is efficient for capturing <i>P. ruralis</i>. Moreover, the cone traps detected <i>P. ruralis</i> earlier than the beating method. The present data results suggest that pheromone-baited cone traps are useful for monitoring <i>P. ruralis</i>.</p></div>","PeriodicalId":8551,"journal":{"name":"Applied Entomology and Zoology","volume":"58 4","pages":"329 - 334"},"PeriodicalIF":1.3,"publicationDate":"2023-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44600877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}