Arkhiv patologii最新文献_第9页

[Undifferentiated pancreatic carcinoma with osteoclast-like giant cells]. [带有破骨细胞样巨细胞的未分化胰腺癌]。

Arkhiv patologii Pub Date : 2023-01-01 DOI: 10.17116/patol20238501162

A Yu Shamanova, D M Rostovtsev, A V Privalov, L V Yarina, K S Aristarkhova, G V Sychugov, I V Vasil'kova, A S Artem'eva, V V Saevets, E A Alymov

引用次数: 0

[Small cell neuroendocrine carcinoma of the endometrium]. [子宫内膜小细胞神经内分泌癌]。

Arkhiv patologii Pub Date : 2023-01-01 DOI: 10.17116/patol20238504159

G A Demyashkin, R F Zibirov, A A Lugovskoi, A A Sigorskaia, I A Stoliarov

引用次数: 0

[Verification of the diagnosis of supratentorial ependymomas by real-time PCR]. [实时荧光定量PCR诊断幕上室管膜瘤的验证]。

Arkhiv patologii Pub Date : 2023-01-01 DOI: 10.17116/patol2023850315

S A Galstyan, E N Telysheva, A O Lavrinovich, E G Shaikhaev, G P Snigireva, E I Petrova, S K Gorelyshev, O G Zheludkova, Y V Kushel, E V Kumirova, M V Ryzhova

{"title":"[Verification of the diagnosis of supratentorial ependymomas by real-time PCR].","authors":"S A Galstyan, E N Telysheva, A O Lavrinovich, E G Shaikhaev, G P Snigireva, E I Petrova, S K Gorelyshev, O G Zheludkova, Y V Kushel, E V Kumirova, M V Ryzhova","doi":"10.17116/patol2023850315","DOIUrl":"https://doi.org/10.17116/patol2023850315","url":null,"abstract":"Background: Differential diagnosis of supratentorial ependymomas is of particular difficulty in neurooncology due to nonspecific clinical and radiographic findings, a rare seen «classic» morphological picture, and a nonspecific immunophenotype. Thanks to molecular genetic methods, in particular real-time PCR, it has become possible to verify supratentorial ependymomas and identify their molecular group, on which further prognosis depends.Objective: To develop a set of molecular genetic tests based on real-time PCR to verify supratentorial ependymomas.Material and methods: 56 tissue samples were collected from patients with supratentorial ependymomas, WHO Grade II, and anaplastic ependymomas, WHO Grade III. We developed primers and fluorescent TaqMan probes for real-time PCR analysis to detect the ZFTA::RELA, ZFTA::MAML2, ZFTA::NCOA2, ZFTA::MAML3, YAP1::MAMLD1, and YAP1::FAM118B gene fusions. For immunohistochemical analysis, monoclonal rabbit anti-NF-kb p65 antibodies (HUABIO, China) were used, the study was carried out on AutostainerLink 48 immunostainer (DAKO, Denmark).Results: Real-time PCR was able to verify the diagnosis for 69.9% (n=39) of samples and classify them into molecular groups of ZFTA- or YAP1-positive supratentorial ependymomas. Immunohistochemically it was possible to verify 58% (n=29) ependymomas.Conclusion: Diagnosis by real-time PCR is a relatively fast, accessible and easily interpreted method that allows verification of the molecular group in 70% of cases of supratentorial ependymomas without the use of additional methods.","PeriodicalId":8548,"journal":{"name":"Arkhiv patologii","volume":"85 3","pages":"5-11"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9577857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Angiomyolipoma of the liver]. [肝脏血管平滑肌脂肪瘤]

Arkhiv patologii Pub Date : 2023-01-01 DOI: 10.17116/patol20238501170

G A Demyashkin, R F Zibirov, O A Anurova, S R Kozaeva, M I Yeronova

引用次数: 1

[Alveolar rhabdomyosarcoma: novel surrogate markers associated with oncogenic translocation]. [肺泡横纹肌肉瘤:与致癌易位相关的新替代标志物]。

Arkhiv patologii Pub Date : 2023-01-01 DOI: 10.17116/patol20238501110

A V Tarakanova, A S Sharlay, D M Konovalov

{"title":"[Alveolar rhabdomyosarcoma: novel surrogate markers associated with oncogenic translocation].","authors":"A V Tarakanova, A S Sharlay, D M Konovalov","doi":"10.17116/patol20238501110","DOIUrl":"https://doi.org/10.17116/patol20238501110","url":null,"abstract":"Background: Anomalies of the FOXO1 gene in alveolar rhabdomyosarcoma are associated with a worse clinical prognosis, which determines the high value of studying the status of this gene when choosing a therapy strategy. The «gold standard» for determining FOXO1 gene rearrangements is currently the fluorescent in situ hybridization (FISH) technique.Objective: Study of the relationship between canonical FOXO1 translocation and immunohistochemical expression of new surrogate markers in alveolar rhabdomyosarcoma to determine their predictive value.Material and methods: 139 cases of rhabdomyosarcoma were retrospectively studied. The study used tissue matrix technology (TMA). On sections obtained from TMA blocks, the FISH technique was implemented using the locus-specific probe MetaSystems XL FOXO1 Break Apart (Metasystems, Germany). Immunohistochemical studies were performed on similar sections from TMA blocks with OLIG2 (Cell Marque Antibodies, clone 211F1.1) and MUC4 (Cell Marque Antibodies, clone 8G7) antibodies.Results: The final expression analysis and statistical processing using a 2x2 contingency table and Fisher's exact test passed 111 cases (76 without FOXO1 rearrangement and 35 with rearrangement). The specificity of OLIG2 and MUC4 expression for FOXO1-rearranged alveolar rhabdomyosarcoma was 85.53% and 80.26%, respectively (p<0.01).Conclusion: The present study confirms the high predictive value of the expression of surrogate markers OLIG2 and MUC4 in determining the genetic status of alveolar rhabdomyosarcoma, which makes it possible to predict with high specificity the detection of the FOXO1 gene rearrangement.","PeriodicalId":8548,"journal":{"name":"Arkhiv patologii","volume":"85 1","pages":"10-15"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10721786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[WHO classification of tumors of hematopoietic and lymphoid tissues, 2022 (5th edition): lymphoid tumors]. [WHO造血和淋巴组织肿瘤分类，2022(第5版):淋巴肿瘤]。

Arkhiv patologii Pub Date : 2023-01-01 DOI: 10.17116/patol20238504124

D V Baram, Z P Asaulenko, I N Spiridonov, Yu A Krivolapov

引用次数: 0

[Detection of SARS-CoV-2 RNA in the mucosa of the appendices of children with COVID-19]. [COVID-19患儿阑尾黏膜SARS-CoV-2 RNA检测]。

Arkhiv patologii Pub Date : 2023-01-01 DOI: 10.17116/patol20238503123

G A Demyashkin, K R Gorokhov, P V Nikitin, I A Zorin, S I Voznesenskaya

{"title":"[Detection of SARS-CoV-2 RNA in the mucosa of the appendices of children with COVID-19].","authors":"G A Demyashkin, K R Gorokhov, P V Nikitin, I A Zorin, S I Voznesenskaya","doi":"10.17116/patol20238503123","DOIUrl":"https://doi.org/10.17116/patol20238503123","url":null,"abstract":"Background. The novel coronavirus infection (COVID-19) often manifests in children as diarrhea, vomiting, abdominal pain, and some children develop acute appendicitis. To elucidate the role of SARS-CoV-2 in the development of acute appendicitis, a more detailed study of the presence of its genetic material in the tissue of the appendix.Objective: Determination of SARS-CoV-2 RNA in appendices of children with COVID-19 by fluorescence in situ hybridization (FISH).Material and methods: A retrospective analysis of case histories and morphological analysis using FISH of appendices of pediatric patients with established clinical diagnosis of acute appendicitis and confirmed infection with SARS-CoV-2 was performed. The material was divided into 3 groups: 1st -appendices obtained during appendectomy in children with established clinical diagnosis of «coronavirus infection» (COVID-19, PCR+) (n=42; mean age 10.8 years); 2nd - appendices of children (n=55; mean age 9.7 years) with acute appendicitis obtained before the onset of the COVID-19 pandemic; 3rd (control) group (n=38; mean age 10.3 years) - autopsy material of the appendices (intact).Results: In all samples of the appendices of the 1st group, a positive SARS-CoV-2 viral RNA signal was noted in the cytoplasm of most epithelial cells and single immunocompetent cells. The signal intensity remained the same in all slides, regardless of age. In all samples obtained from patients without COVID-19 (groups 2 and 3), confocal microscopy did not reveal a signal, which indicates successful adaptation of the FISH method in this study and excludes the false positive results.Conclusion: In the epithelium of the appendices of children of different age with COVID-19, the FISH method revealed SARS-CoV-2 RNA, which does not exclude the association between viral invasion and the development of acute appendicitis.","PeriodicalId":8548,"journal":{"name":"Arkhiv patologii","volume":"85 3","pages":"23-28"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9949912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Atypical fibroxanthoma]. [非典型纤维黄色瘤]。

Arkhiv patologii Pub Date : 2023-01-01 DOI: 10.17116/patol20238505165

N V Vasilyev, S V Vtorushin, A A Maltseva, A V Sannikova

引用次数: 0

[Corneal changes in Fuchs endothelial corneal dystrophy and bullous keratopathy]. 【Fuchs内皮性角膜营养不良和大泡性角膜病变的角膜变化】。

Arkhiv patologii Pub Date : 2023-01-01 DOI: 10.17116/patol20238505129

T A Demura, N V Fisenko, G A Osipyan, M A Afonina

{"title":"[Corneal changes in Fuchs endothelial corneal dystrophy and bullous keratopathy].","authors":"T A Demura, N V Fisenko, G A Osipyan, M A Afonina","doi":"10.17116/patol20238505129","DOIUrl":"10.17116/patol20238505129","url":null,"abstract":"Objective: Evaluation of structural and immunohistochemical features of cornea in Fuchs endothelial corneal dystrophy (FECD) and bullous keratopathy (BK).Material and methods: Group 1 - 44 patients (46 eyes) with FECD, group 2 - 42 patients (42 eyes) with BK. All patients underwent keratoplasty. Preoperative anterior segment optical coherence tomography (AS-OCT, RTVue-100, Optovue, USA) was performed. Endothelium-Descemet membrane (EDM) complexes, corneal buttons were obtained intraoperatively. Morphological (H&E staining) and immunohistochemical (primary antibodies to pancytokeratin, vimentin, fibronectin) studies were performed at the light microscope level (Leica DM-2500, Leica Application Suite V4.8, Leica Microsystems, Switzerland).Results: A direct correlation is found between the results of DM analysis in vivo with OCT and ex vivo with light microscopy. DM thickness (AS-OCT) was significantly greater in FECD (23.0 [19.0; 27.0] μm), than in BK (13.0 [12.0; 14.0] μm). Morphological study of EDM and corneal buttons showed similar difference in DM thickness: 17.9 [16.1; 20.0] μm in FECD and 11.9 [11.3; 13.0] μm in BK. Irregular optical density of stroma is a feature of edema and local fibrosis. In FECD and BK pancytokeratin is expressed in epithelial and endothelial cells, vimentin - in keratocytes, macrophages and vascular endothelium, fibronectin - in DM. In FECD, vimentin is expressed in endothelial cells.Conclusion: FECD and BK are associated with different DM' and endothelium' abnormalities, which lead to similar changes of stroma and epithelium. AS-OCT is a useful method of FECD and BK in vivo diagnostics and the selection of treatment option.","PeriodicalId":8548,"journal":{"name":"Arkhiv patologii","volume":"85 5","pages":"29-35"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41181924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Molecular mechanisms of impaired antigenic presentation as a cause of tumor escape from immune surveillance]. [抗原呈递受损作为肿瘤逃避免疫监视原因的分子机制]。

Arkhiv patologii Pub Date : 2023-01-01 DOI: 10.17116/patol20238506176

A A Korotaeva, A A Borunova, A Yu Kuzevanova, T N Zabotina, A A Alimov

引用次数: 0