Arkhiv patologii最新文献

{"title":"[Neuroblastoma: prevalence of genetic alterations in different histological groups].","authors":"M A Baranova, A S Sharlai, D M Konovalov","doi":"10.17116/patol2025870615","DOIUrl":"10.17116/patol2025870615","url":null,"abstract":"<p><p>Neuroblastoma is a disease characterized by clinical, histological and genetic heterogeneity. The morphological type, immunohistochemical expression of proteins (MYCN, MYC) and molecular genetic characteristics of the tumor (the status of the <i>MYCN</i> gene and chromosomal regions 1p and 11q) are key to predicting the course of the disease and choosing therapy.</p><p><strong>Objective: </strong>Estimate the prevalence of the most clinically significant genetic events in different histological groups and among neuroblastomas in general and to demonstrate rarer genetic findings.</p><p><strong>Material and methods: </strong>A total of 233 cases of patients with neuroblastoma, for whom the histological conclusion and the results of cytogenetic examination by FISH (the status of the <i>MYCN</i> gene and chromosomal regions 1p and 11q) were known, were analyzed. Based on these data, the cases were divided into favorable and unfavorable histological groups, in each of which the presence of genetic abnormalities was analyzed. Also, 28 cases of patients with large cell neuroblastoma were analyzed, for whom the histological conclusion, <i>MYCN</i> gene status, immunohistochemical expression of MYC protein and the results of cytogenetic study of <i>MYC</i> gene status by FISH were known.</p><p><strong>Results: </strong><i>MYCN</i> gene amplification, 1p deletion/imbalance and 11q deletion/imbalance are more common in tumors from the unfavorable morphological group. Aberrations of the chromosomal region 1p and <i>MYCN</i> amplification are events that often occur together, while 11q aberrations, on the contrary, have an inverse correlation with <i>MYCN</i> amplification. 11q deletion/imbalance is more often detected in tumors with <i>MYCN</i> gain. In 4 cases, 1p and 11q chromosomal aberrations were detected in the same tumor, all in the unfavorable histological group. Two cases with chromosomal aberrations in stromal cells were also identified. Expression of MYC protein was detected in 5 cases, of which 2 showed amplification of the <i>MYC</i> gene.</p>","PeriodicalId":8548,"journal":{"name":"Arkhiv patologii","volume":"87 6","pages":"5-11"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145653266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Viktor Viktorovich Serov. On the 100th anniversary of his birth].","authors":"S G Radenska-Lopovok, D D Protsenko","doi":"10.17116/patol20258702188","DOIUrl":"https://doi.org/10.17116/patol20258702188","url":null,"abstract":"<p><p>On December 5, 2024 it was 100 years since the birth of Viktor Viktorovich Serov, Doctor of Medical Sciences, Professor, Academician of the Russian Academy of Medical Sciences.</p>","PeriodicalId":8548,"journal":{"name":"Arkhiv patologii","volume":"87 2","pages":"88-89"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144061959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The effect of acetylamide synthetic peptide HLDF-6 on cell differentiation in breast cancer].","authors":"A A Studenikina, E D Mangazeeva, A P Bogachuk, V M Lipkin, A I Autenshlyus","doi":"10.17116/patol2025870215","DOIUrl":"https://doi.org/10.17116/patol2025870215","url":null,"abstract":"<p><p>The development of drugs with the ability to increase differentiation and reduce tumor malignancy is one of the promising directions in the treatment of breast cancer (BC). As such, Human Leukemia Differentiation Factor (HLDF), a protein consisting of 54 amino acids and contributing to an increase in the degree of differentiation of invasive ductal breast carcinoma cells, can be used. The key disadvantage of the full-size HLDF is its rapid biodegradation. In this connection, the acetylamide form of the peptide (HLDF-6) was synthesized to protect against hydrolysis.</p><p><strong>Objective: </strong>To evaluate the effect of HLDF-6 on cell differentiation in various molecular biological subtypes of breast cancer.</p><p><strong>Material and methods: </strong>The relative content of tumor cells of various degrees of differentiation in biopsies of 33 women with breast cancer when exposed to HLDF-6 tumor was evaluated.</p><p><strong>Results: </strong>HLDF-6 significantly increases the relative content of differentiated cells in the luminal B HER2-negative subtype of breast cancer, without significantly affecting the cells of patients with luminal A and basal-like subtypes.</p><p><strong>Conclusion: </strong>The study revealed the prospects of using HLDF-6 as part of differentiating therapy in patients with luminal B HER2-negative subtype of breast cancer.</p>","PeriodicalId":8548,"journal":{"name":"Arkhiv patologii","volume":"87 2","pages":"5-10"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Morphological markers of mitochondrial dysfunction of the basal plate cytotrophoblast of the placenta in preeclampsia of varying severity].","authors":"Z S Khizrieva, L V Kulida, I A Panova","doi":"10.17116/patol20258702124","DOIUrl":"https://doi.org/10.17116/patol20258702124","url":null,"abstract":"<p><p>Pathomorphological changes in preeclampsia develop in all elements of the functional system \"mother-placenta-fetus\" and play a leading role in the course and outcome of pregnancy.</p><p><strong>Objective: </strong>To identify morphological markers of mitochondrial dysfunction of the basal plate cytotrophoblast of the placenta in women with preeclampsia of varying severity.</p><p><strong>Material and methods: </strong>Comprehensive morphological examination of placentas from women with uncomplicated pregnancy (30), moderate (30) and severe (30) preeclampsia included overview histology, morphometry, immunohistochemistry and transmission electron microscopy.</p><p><strong>Results: </strong>Regardless of the severity of preeclampsia, a leading place in the pathomorphological change in the placenta is occupied by a violation of uteroplacental circulation due to a violation of cytotrophoblast invasion. Immunohistochemical parameters of mitochondrial dysfunction of cytotrophoblast cells of the basal lamina have been determined. A significant decrease in the expression of mtDNA transcription and replication proteins (TFAM, POLG) and a violation of the structure of the respiratory chain (NADH+, cytochrome C) were revealed. These changes were combined with ultrastructural rearrangement of mitochondria, which are the basis of ATP synthesis. In moderate preeclampsia, a decrease in the number of mitochondria was revealed, the ratio of oval and rounded forms of mitochondria associated with swelling of the central parts of the crist changes. In severe preeclampsia, the structural basis of the connection of energy metabolism between mitochondria and the granular endoplasmic reticulum is interrupted. The progressive destruction of mitochondrial crysts with the formation of pseudovesicles ends with total necrosis of ultrastructures.</p><p><strong>Conclusion: </strong>In pregnant women with preeclampsia, mitochondrial dysfunction of the cytotrophoblast cells of the basal lamina was diagnosed, characterized by a decrease in the expression of mtDNA transcription and replication proteins in combination with a violation of the I-III enzymatic complexes of the respiratory chain. The identified immunohistochemical markers are pathogenetically associated with ultrastructural rearrangement of mitochondria in preeclampsia of varying severity.</p>","PeriodicalId":8548,"journal":{"name":"Arkhiv patologii","volume":"87 2","pages":"24-29"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143959864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Seminoma of undescended testis in a patient with false hermaphroditism].","authors":"A D Tsaregorodtseva, Yu A Tikhonova, A A Shishkina, A S Smirnov, A V Arnautov, K A Nikulina, S A Reva, S B Petrov, A K Musonova, V D Nazarov, M G Rybakova","doi":"10.17116/patol20258704148","DOIUrl":"10.17116/patol20258704148","url":null,"abstract":"<p><p>Disorders of sex development or hermaphroditism are a condition associated with a discrepancy between genetic, gonadal, and phenotypic sex. Persistent Müllerian duct syndrome (PMDS) is a rare form of male pseudohermaphroditism. Typical features are cryptorchidism and the presence of underdeveloped fallopian tubes, uterus or upper vagina in a male with karyotype 46, XY. Over the past 50 years, about 200 cases of persistent Müllerian duct syndrome have been reported. The article describes an observation of a 33-year-old patient with bilateral cryptorchidism, testicular neoplasm, and false hermaphroditism revealed during the examination of surgical material. Macroscopic and microscopic pictures are presented, including those using immunohistochemical methods. The results of a molecular genetic study of the surgical material are given. After all the examinations, the patient was diagnosed with: Typical seminoma of the right undescended testicle, pT2. False hermaphroditism.</p>","PeriodicalId":8548,"journal":{"name":"Arkhiv patologii","volume":"87 4","pages":"48-53"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144940572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The impact of combined immunotherapy on the cellular composition of the tumor microenvironment in patients with gastric carcinoma].","authors":"L A Tashireva, A Yu Kalinchuk, D M Loos, E A Tsarenkova, A V Avgustinovich, S G Afanas'ev, S V Vtorushin","doi":"10.17116/patol20258704124","DOIUrl":"10.17116/patol20258704124","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the impact of combined anti-PD-1 immunotherapy on the cellular composition of the tumor microenvironment in patients with gastric cancer.</p><p><strong>Material and methods: </strong>The study included 9 patients with morphologically confirmed gastric adenocarcinoma (stages T2-4N0-1M0) and positive PD-L1 status (CPS >1). All patients received 8 courses of preoperative chemotherapy according to the FLOT regimen, combined with additional immunotherapy using pembrolizumab (400 mg every 6 weeks). Changes in the tumor microenvironment were assessed using multicolor immunofluorescence followed by quantitative analysis of the proportions of CD8⁺ cytotoxic T-lymphocytes, CD20⁺ B-lymphocytes, CD163⁺ macrophages, and FoxP3⁺ regulatory T-cells.</p><p><strong>Results: </strong>A statistically significant increase in the number of CD8⁺ T-lymphocytes (from 3.18% to 6.23%, <i>p</i>=0.0391) and, in particular, their PD-1-expressing subpopulation (from 0.00% to 0.22%, <i>p</i>=0.0156) was observed, while the numbers of CD20⁺, CD163⁺, and FoxP3⁺ cells remained unchanged. As a result, the CD8⁺/FoxP3⁺ cell ratio significantly increased during combined anti-PD-1 immunotherapy (from 0.69 to 3.22, <i>p</i>=0.0039). Additionally, associations were identified between immune parameters and clinicopathological characteristics, suggesting that gender and tumor stage may influence the immune response.</p><p><strong>Conclusion: </strong>The obtained data indicate a remodeling of the tumor microenvironment under the influence of anti-PD-1 therapy, which potentially enhances the antitumor immune response and opens prospects for further optimization of gastric cancer treatment.</p>","PeriodicalId":8548,"journal":{"name":"Arkhiv patologii","volume":"87 4","pages":"24-30"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144940534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Microsatellite instability as a possible diagnostic marker of the gastric mucosa dysplasia].","authors":"A V Kononov, V A Rubtsov, M N Parygina, E V Demidova, S I Mozgovoi, E G Pomorgailo, A G Shimanskaya, M V Markelova","doi":"10.17116/patol2025870315","DOIUrl":"https://doi.org/10.17116/patol2025870315","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the MMR system proteins and the MSI status of regenerative (indefinite for dysplasia) and pronounced (epithelial dysplasia) gastric mucosa precancerous lesions in the comparison with cancer to determine their possible potential as a diagnostic markers of gastric mucosa dysplasia.</p><p><strong>Material and methods: </strong>The study included 2 groups of gastric mucosa specimens: (1) 150 biopsy gastric mucosa specimens: 43 with low-grade dysplasia, 32 with high-grade dysplasia, 75 - indefinite for dysplasia; (2) 155 cancer tissue specimens from resected stomachs. Gastric mucosa specimens were examined histologically, immunohistochemically using mouse monoclonal antibodies to the MMR system proteins: MLH-1, MSH2, MSH6, PMS2 (Diagnostic BioSystems, USA). MSI was studied with multiplex PCR evaluation of DNA microsatellites (NR-21, NR-24, NR-27, BAT-25, BAT-26) from paraffin sections, their analysis with capillary electrophoresis. The obtained data were processed with the Statistica 10.0 (StatSoft, USA), presented using descriptive, analytical statistics.</p><p><strong>Results: </strong>MSI was detected by immunohistochemistry in 8% (<i>n</i>=6) of the studied cases of low/high grade dysplasia, which does not have statistically significant differences from the distribution of MSI in the gastric cancer group (12% of microsatellite-unstable cases (<i>n</i>=18)) (<i>p</i>=0.49). MSI was not detected in any indefinite for dysplasia case.</p><p><strong>Conclusion: </strong>Detection of microsatellite instability in gastric mucosa dysplasia indicates the likelihood of its occurrence at the early stages of the carcinogenesis cascade and makes it possible to use it to assess the risk of gastric cancer associated with microsatellite instability. The absence of instability in cases indefinite for dysplasia determines the possibility of its use for differential diagnosis of truly neoplastic and regenerative/reactive changes in the gastric mucosa.</p>","PeriodicalId":8548,"journal":{"name":"Arkhiv patologii","volume":"87 3","pages":"5-16"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144324383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Pathomorphological characteristics of inflammatory and hereditary myopathies].","authors":"I S Limaev, N S Gladyshev, A M Emelin, Yu A Vetrova, S N Bardakov, R V Deev","doi":"10.17116/patol20258704131","DOIUrl":"10.17116/patol20258704131","url":null,"abstract":"<p><strong>Objective: </strong>To characterize the immunophenotypic features of the inflammatory infiltrate cell composition and the morphometric features of muscle fibers in skeletal muscle biopsies from patients with hereditary and inflammatory myopathies, and to develop an integral coefficient to aid in the differential diagnosis of these conditions.</p><p><strong>Material and methods: </strong>The material is represented by biopsy specimens of m. tibialis anterior, m. vastus lateralis, m. gastrocnemius med, m. peroneus longus et brevis, m. deltoideus, m. biceps femoris and m. latissimus dorsi of patients with polymyositis (<i>n</i>=7), dermatomyositis (<i>n</i>=3), dysferlinopathy (<i>n</i>=10) and calpainopathy (<i>n</i>=3), established on the basis of molecular-genetic, clinical-instrumental and morphological data. All biopsies underwent pathohistological and immunohistochemical examination.</p><p><strong>Results: </strong>The number of necrotic muscle fibers was significantly higher in polymyositis compared to dermatomyositis (<i>p</i>=0.008), dysferlinopathy (<i>p</i>=0.003), and calpainopathy (<i>p</i>=0.009), showing a diffuse pattern. In dermatomyositis, necrotic muscle fibers were predominantly perifascicular. In dysferlinopathy, a positive correlation between CD68⁺-macrophages and CD4⁺-T-helpers in the perimysium (<i>p</i>=0.04) were observed. The number of CD8⁺-T-killer cells invading muscle fibers was higher in polymyositis compared to dysferlinopathy (<i>p</i>=0.034). Increased numbers of CD138⁺-plasma cells was also noted in polymyositis. The <i>MICE</i> coefficient was lower in hereditary myopathies.</p><p><strong>Conclusion: </strong>Immunophenotyping of the inflammatory infiltrate and quantitative morphometry using the integral <i>MICE</i> coefficient provide criteria for the differential diagnosis of myopathies of different origins. The established differences in the cellular composition of the infiltrate (particularly, the predominance of invasion by CD8⁺-T-killers in polymyositis) and in the degree of morphological homogeneity of muscle fibers (higher in hereditary forms) represent objective differential diagnostic criteria. Thus, the integrated application of these approaches can significantly improve the accuracy of verifying the differential diagnosis in myopathies of various origins.</p>","PeriodicalId":8548,"journal":{"name":"Arkhiv patologii","volume":"87 4","pages":"31-39"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144940579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Spectrum of morphological changes in chronic spontaneous urticaria and urticarial vasculitis].","authors":"V A Smolyannikova, O Yu Olisova, K S Teplyakova, A V Filatov, N A Larin","doi":"10.17116/patol20258702130","DOIUrl":"https://doi.org/10.17116/patol20258702130","url":null,"abstract":"<p><p>The prevalence of chronic spontaneous urticaria (CSU) in the population is 0.5%-1%. According to modern guidelines for the management of patients with urticaria, the diagnosis of CSU does not require histological examination. However, in controversial clinical setting requiring differential diagnosis with urticarial vasculitis (UV), pathologists discover a wide range of pathomorphological changes in skin preparations in the absence of generally accepted differential diagnostic criteria. In this connection, it is of interest to study the spectrum of morphological changes in chronic spontaneous urticaria and urticarial vasculitis.</p><p><strong>Objective: </strong>To analyze morphological changes in CSU and UV to improve differential diagnosis in clinical practice.</p><p><strong>Material and methods: </strong>The material of 15 patients with urticarial rashes was analyzed. Comparative analysis of the number of neutrophils and mast cells in skin samples from patients with chronic spontaneous urticaria and urticarial vasculitis was performed.</p><p><strong>Results: </strong>The material of 15 patients with urticarial rashes was analyzed. The group of patients with CSU (<i>n</i>=5) was characterized by the absence of signs of leukocytoclastic vasculitis; endothelial cells and mild of moderate swelling were visualized in all samples, the perivascular infiltrate was located in the upper layer of the dermis and was sparse. And when stained with toluidine blue, a large number of mast cells were noted. In the group of patients with UV (<i>n</i>=10), signs of leukocytoclastic vasculitis of varying severity, dense and deep perivascular neutrophilic infiltrates with a small number of mast cells were visualized.</p><p><strong>Conclusion: </strong>Considering the wide range of morphological changes in the skin in CSU and UV, for the differential diagnosis of diseases, a comprehensive histological examination of preparations in combination with an assessment of the number of mast cells in the dermis and an assessment of the composition of the cellular infiltrate for the number of neutrophils can be recommended.</p>","PeriodicalId":8548,"journal":{"name":"Arkhiv patologii","volume":"87 2","pages":"30-36"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143959865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Frequency of MSI, PD-L1 (CPS), HER2 in poorly cohesive gastric carcinomas].","authors":"S N Nered, R O Torosyan, N A Kozlov, H Sun, I G Avdyukhin, P V Kononets, I S Stilidi","doi":"10.17116/patol20258702111","DOIUrl":"https://doi.org/10.17116/patol20258702111","url":null,"abstract":"<p><p>Gastric cancer (GC) is a heterogeneous tumor with various molecular changes. An active search for molecular markers is crucial to determine the effectiveness of drug treatment and prognosis of the disease. Several biomarkers have the greatest clinical significance: HER2, MSI / dMMR, PD-L1 (CPS), EBV and Claudin 18.</p><p><strong>Objective: </strong>To study the frequency of HER2, MSI / dMMR and PD-L1 (CPS) in patients with operable GC depending on the tumor type according to P. Lauren.</p><p><strong>Material and methods: </strong>The study included 600 patients with GC who underwent radical surgical treatment at the N.N. Blokhin National Medical Research Center of Oncology from 2018 to 2023.</p><p><strong>Results: </strong>In the study, the proportion of diffuse GC was 21.5% (120/600). HER2 overexpression was found in 5.2% (5/96) of cases with diffuse GC, 20.4% (37/181) of cases with intestinal GC, 10.1% (7/69) of cases with mixed GC (<i>p</i>=0.0029). The incidence of MSI was 3.3% (4/120) of cases with diffuse GC, 11.2% (28/251) of cases with intestinal GC, 7.3% (7/97) of cases with mixed GC (<i>p</i>=0.0378). PD-L1 expression (CPS> 1) was found in 42.3% (11/26) of cases with diffuse GC, 59.4% (35/59) of cases with intestinal GC, 27.3% (9/33) of cases with mixed GC (<i>p</i>=0.006). In poorly cohesive/signet ring cell cancer MSI occurred in 2.5% (2/79) of cases; HER2 overexpression - in 2.9% (2/68) of cases; PD-L1 (CPS> 1) - in 42.1% (8/19) of cases.</p><p><strong>Conclusion: </strong>Our study demonstrated that in diffuse and poorly cohesive/signet ring cell GC the frequency of occurrence of the above clinically significant tumor biomarkers is lower compared to intestinal/mixed GC.</p>","PeriodicalId":8548,"journal":{"name":"Arkhiv patologii","volume":"87 2","pages":"11-17"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}