Laboratory hematology : official publication of the International Society for Laboratory Hematology最新文献

Antineutrophil cytoplasmic antibody-associated neutropenia in a patient with type 1 diabetes mellitus. 1型糖尿病患者抗中性粒细胞细胞质抗体相关的中性粒细胞减少症。

Laboratory hematology : official publication of the International Society for Laboratory Hematology Pub Date : 2014-06-01 DOI: 10.1532/LH96.13001

Caner Saygin, Didem Uzunaslan, Serdal Ugurlu

引用次数: 2

Erythrocyte phosphatidylserine exposure in β-thalassemia. β-地中海贫血的红细胞磷脂酰丝氨酸暴露。

Laboratory hematology : official publication of the International Society for Laboratory Hematology Pub Date : 2014-06-01 DOI: 10.1532/LH96.12016

Hamdy A Ibrahim, Manal I Fouda, Raida S Yahya, Nashwa K Abousamra, Rania A Abd Elazim

{"title":"Erythrocyte phosphatidylserine exposure in β-thalassemia.","authors":"Hamdy A Ibrahim, Manal I Fouda, Raida S Yahya, Nashwa K Abousamra, Rania A Abd Elazim","doi":"10.1532/LH96.12016","DOIUrl":"https://doi.org/10.1532/LH96.12016","url":null,"abstract":"[ABS]Phospholipid asymmetry is well maintained in erythrocyte (RBC) membranes with phosphatidylserine (PS) exclusively present in the inner leaflet. Eryptosis, the suicidal death of RBCs, is characterized by cell shrinkage, membrane blebbing, and cell membrane phospholipids scrambling with PS exposure at the cell surface. Erythrocytes exposing PS are recognized, bound, engulfed, and degraded by macrophages. Eryptosis thus fosters clearance of affected RBCs from circulating blood, which may aggravate anemia in pathological conditions. Thalassemia patients are more sensitive to the eryptotic depletion and osmotic shock which may affect RBC membrane phospholipid asymmetry. We aimed in this work to determine the RBC PS exposure in splenectomized and nonsplenectomized β-thalassemia major (β-TM) patients and correlate it with the clinical presentation and laboratory data. RBCs were stained for annexin V to detect phosphatidylserine (PS) exposure in 46 β-TM patients (27 splenectomized and 19 nonsplenectomized) compared to 17 healthy subjects as a control group. We observed a significant increase in RBC PS exposure in β-TM patients compared to control group (P = .0001). Erythrocyte PS exposure was significantly higher in splenectomized β-TM patients compared with nonsplenectomized β-TM patients (P = .001). No correlation was found between RBC PS exposure and clinical or hematological data of β-TM patients, but there was a positive correlation between RBC PS exposure and ferritin level in β-TM patients have higher levels of RBC PS exposure, and splenectomy was shown to aggravate RBC PS exposure without aggravation of anemia. ","PeriodicalId":85078,"journal":{"name":"Laboratory hematology : official publication of the International Society for Laboratory Hematology","volume":"20 2","pages":"9-14"},"PeriodicalIF":0.0,"publicationDate":"2014-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32484755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

Induction of fetal hemoglobin as a novel therapeutic strategy for β-hemoglobinopathy. 诱导胎儿血红蛋白作为一种治疗β-血红蛋白病的新策略。

Laboratory hematology : official publication of the International Society for Laboratory Hematology Pub Date : 2014-03-01 DOI: 10.1532/LH96.12015

Ali Dehghani Fard, Saeid Kaviani, Mehrdad Noruzinia, Najmaldin Saki, Esmaeil Mortaz

引用次数: 4

Rapid separation of mononuclear hodgkin from multinuclear reed-sternberg cells. 单核霍奇金细胞与多核reed-sternberg细胞的快速分离。

Laboratory hematology : official publication of the International Society for Laboratory Hematology Pub Date : 2014-03-01 DOI: 10.1532/LH96.12023

Narisorn Kongruttanachok, Yvon E Cayre, Hans Knecht, Sabine Mai

引用次数: 2

Characteristic scattergram of white blood cells obtained using the Pentra MS CRP hematology analyzer in a patient with neutral lipid storage disease. 中性脂质储存病患者使用Pentra MS CRP血液学分析仪获得的白细胞特征散点图。

Laboratory hematology : official publication of the International Society for Laboratory Hematology Pub Date : 2013-12-01 DOI: 10.1532/LH96.12022

Tohru Inaba, Naoyuki Nomura, Mayako Takahashi, Katsutoshi Ishizuka, Kazunori Yoshika, Soichi Yuasa, Masaki Nakanishi, Naohisa Fujita

引用次数: 6

Pathogenetic role of Factor VII deficiency and thrombosis in cross-reactive material positive patients. 因子7缺乏和血栓形成在交叉反应物质阳性患者中的发病作用。

Laboratory hematology : official publication of the International Society for Laboratory Hematology Pub Date : 2013-12-01 DOI: 10.1532/LH96.12011

A Girolami, L Sambado, E Bonamigo, S Ferrari, A M Lombardi

引用次数: 3

The role of the nervous system in hematopoietic stem cell mobilization. 神经系统在造血干细胞动员中的作用。

Laboratory hematology : official publication of the International Society for Laboratory Hematology Pub Date : 2013-09-01 DOI: 10.1532/LH96.12013

Fakhredin Saba, Masoud Soleimani, Amir Atashi, Esmaeil Mortaz, Mohammad Shahjahani, Elham Roshandel, Kaveh Jaseb, Najmaldin Saki

{"title":"The role of the nervous system in hematopoietic stem cell mobilization.","authors":"Fakhredin Saba, Masoud Soleimani, Amir Atashi, Esmaeil Mortaz, Mohammad Shahjahani, Elham Roshandel, Kaveh Jaseb, Najmaldin Saki","doi":"10.1532/LH96.12013","DOIUrl":"https://doi.org/10.1532/LH96.12013","url":null,"abstract":"Hematopoietic stem cells (HSCs) and blood cell progenitors, such as maturing leucocytes, steadily enter from bone marrow (BM) into the circulation under steady-state conditions, and their mobilization is dramatically amplified during stress conditions and by mediators such as granulocyte colony-stimulating factor (G-CSF). This mobilization is dependent upon bone remodeling, the proteolytic enzymes of bone marrow-derived stromal cells, and adhesion molecules such as integrin, but the main mechanisms controlling this traffic are still unclear. The nervous system, as the most important regulator of the body, can affect the mobilization network by secreting catecholamines, so that denervation of catecholaminergic fibers in the BM of mice could lead to declining mobilization in steady state and stress situations, even in the presence of other intact environmental factors in the BM. Thus, due to the importance of the nervous system, we have attempted to give a general overview of how the nervous system is involved in the mobilization of HSCs in this review. Then, we will try to describe the mobilization process induced by the nervous system, which consists of 3 mechanisms: stromal cell-derived factor 1 (SDF-1)/CXC chemokine receptor type 4 (CXCR4), proteolytic enzymes, and bone remodeling. ","PeriodicalId":85078,"journal":{"name":"Laboratory hematology : official publication of the International Society for Laboratory Hematology","volume":"19 3","pages":"8-16"},"PeriodicalIF":0.0,"publicationDate":"2013-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1532/LH96.12013","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40272468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 16

Adult and pediatric cases of B-cell lymphoblastic leukemia differ in the clinical significance of CD66c expression. 成人与儿童b细胞白血病患者CD66c表达的临床意义存在差异。

Laboratory hematology : official publication of the International Society for Laboratory Hematology Pub Date : 2013-06-01 DOI: 10.1532/LH96.12014

Tohru Inaba, Hiroshi Nishimura, Akiko Kakae, Junko Saito, Yoko Yamane, Mamoru Ishizawa, Toshihiko Imamura, Shin-ichi Fuchida, Ryoichi Takahashi, Naohisa Fujita

引用次数: 0

Evaluation of H3 histone methylation and colony formation in erythroid progenitors treated with thalidomide and sodium butyrate. 沙利度胺和丁酸钠对红系祖细胞H3组蛋白甲基化和集落形成的影响。

Laboratory hematology : official publication of the International Society for Laboratory Hematology Pub Date : 2013-03-01 DOI: 10.1532/LH96.12003

Ali Dehghani Fard, Saeid Kaviani, Mehrdad Noruzinia, Masoud Soleimani, Saeid Abroun, Rouzbeh Chegeni, Abbas Hajifathali, Zahra Zonoubi, Mohammad Ahmadvand, Majid Mossahebi Mohammadi, Najmaldin Saki

{"title":"Evaluation of H3 histone methylation and colony formation in erythroid progenitors treated with thalidomide and sodium butyrate.","authors":"Ali Dehghani Fard, Saeid Kaviani, Mehrdad Noruzinia, Masoud Soleimani, Saeid Abroun, Rouzbeh Chegeni, Abbas Hajifathali, Zahra Zonoubi, Mohammad Ahmadvand, Majid Mossahebi Mohammadi, Najmaldin Saki","doi":"10.1532/LH96.12003","DOIUrl":"https://doi.org/10.1532/LH96.12003","url":null,"abstract":"Objectives: β-thalassemia and sickle cell disease are hemoglobinopathies with reduced/absent β chains in the former and dysfunctional β chains in the latter. In both conditions, up-regulation of hemoglobin F through demethylation can alleviate the symptoms. This can be attained with drugs such as thalidomide and sodium butyrate.Materials and methods: This study was performed on erythroid progenitors derived from CD133+ cord blood stem cells. Erythroid progenitors were treated with thalidomide and sodium butyrate in single and combined groups. Colony-formation potential in each group was evaluated by the colony assay. Real-time polymerase chain reaction (RT-PCR) was used to evaluate the effect of these drugs on histone H3 lysine 27 (H3K27) methylation patterns.Findings: Compared to other treatment groups, CD133+ cells treated with thalidomide alone produced more hematopoietic colonies. Thalidomide alone was also more effective in decreasing H3K27 methylation.Conclusions: Thalidomide shows superiority to sodium butyrate as a hypomethylating agent in this cell culture study, and it has the potential to become conventional treatment for sickle cell disease and β-thalassemia.","PeriodicalId":85078,"journal":{"name":"Laboratory hematology : official publication of the International Society for Laboratory Hematology","volume":"19 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2013-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40233477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

The incidence of co-existing BCR-ABL1 and JAK2 V617F rearrangements: implications for molecular diagnostics. BCR-ABL1和JAK2 V617F重排共存的发生率:对分子诊断的影响

Laboratory hematology : official publication of the International Society for Laboratory Hematology Pub Date : 2012-12-01 DOI: 10.1532/LH96.12009

Sarah L McCarron, Karl Haslam, Mireille Crampe, Stephen E Langabeer

引用次数: 0