Wali Muhammad, M. Ammar, T. Mukhtar, Abdulmaula Hamza
{"title":"Comparative Efficacy of Some New Chemistry Foliar Insecticides against Cotton Whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae)","authors":"Wali Muhammad, M. Ammar, T. Mukhtar, Abdulmaula Hamza","doi":"10.35495/ajab.2021.02.075","DOIUrl":"https://doi.org/10.35495/ajab.2021.02.075","url":null,"abstract":"Cotton whitefly (Bemisia tabaci) is known as the most destructive pest of vegetables, ornamentals and cotton including a wide range of weeds, forest trees and semi field crops in the world. Cotton production and area under cotton is continuously declining in Pakistan for the last five years due to a rapid proliferation of the pests. The current study was conducted to evaluate the efficacy of some new chemistry insecticides against cotton whitefly (Bemisia tabaci) during the kharif season of 2019 in a major cotton producing area of Punjab, Pakistan. A total of ten insecticides (single or mixtures) i.e. Dinotefuran + Spirotetramat (T1), Dinotefuran + Spirotetramat (T2), Pyriproxyfen+ Spirotetramat (T3), Pyriproxyfen+ Spirotetramat (T4), Spirotetramat (T5), Spirotetramat (T6), Pyrifluquinazon (T7), Pyrifluquinazon (T8), Flonicamid (T9), Flonicamid + Abamectin (T10) were applied twice in a field experiment on two varieties of cotton (MNH-90 and BS-18) using randomized complete block design. Water (T0) served as control. There were three replications of each treatment. Data for population of whitefly (nymphs and adults), natural enemies and boll weight were recorded 3, 7, 10 and 14 days after treatment of insecticides. Pyrifluquinazon @ 48.7 g/acre (T8) caused the maximum mortality (83%) of adults of whitefly while the maximum nymphal mortality (78.4 %) was observed in T8 (Pyrifluquinazon @ 48.7 g/acre). The maximum increase in boll weight (133%) was observed in T8 (Pyrifluquinazon @ 48.7 g/acre) followed by T10 (Flonicamid+Abamectin) which gave 110% increase over control. The application of Pyrifluquinazon @ 40 g/acre was found to be safer as compared with other tested insecticides with the minimum reduction of 31% in the population of natural enemies. It is therefore concluded that Spirotetramat and Pyrifluquinazon can be successfully included in IPM programs against Bemisia tabaci.","PeriodicalId":8506,"journal":{"name":"Asian Journal of Agriculture and Biology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48944585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Synergistic role of pipronyl butoxide in improving the Spodoptera littoralis nucleopolyhedrosis virus effectiveness against Spodoptera littoralis and studying its histopathological biosafety","authors":"Yasmein A. El Sayed, Rania A. Ahmed, S. Desoky","doi":"10.35495/ajab.2021.05.227","DOIUrl":"https://doi.org/10.35495/ajab.2021.05.227","url":null,"abstract":"Spodoptera littoralis nucleopolyhedrosis virus (SlNPV) has been considered as a vital and safe alternative to chemical insecticides. It is effectively used in biological control of Spodoptera littoralis which is considered the most destructive and detrimental economic pest in Egypt. However, its slow speed of action limits its application among other bio-control agents. So, great attention was directed to solve this problem by using insecticide synergists. In this study the toxicity of both SlNPV and the insecticide synergist pipronyl butoxide (PBO) was investigated against 2 nd and 4 th instar S. littoralis larvae. Different concentrations of the tested viral isolate used (10 6 , 10 7 , 10 8 , 10 9 and 10 10 POB/ml) showed high mortality percentage ranging from 60 to 93% and 56 to 83% for the two tested instar larvae respectively 10 days post treatment. The three PBO concentrations used (0.1, 0.2 and 0.3%) revealed weak toxic effect as they produced 13, 20 and 26% mortality rate for the three tested concentrations respectively 4 days post treatment. To examine the biosafety of the three PBO concentrations (0.1, 0.2 and 0.3%), alone or in combination with SlNPV; in vivo animal studies, and in vitro cell line studies were applied. In vivo evaluations included histomorphometric and immunohistochemical alterations in Caspase-3, Proliferating cell nuclear antigen (PCNA), tumor necrosis factor – alpha (TNF-α) in hepatic and renal tissues, plus serum assessments of malondialdehyde (MDA) and total antioxidant capacity (TAC). The sulforhodamine B (SRB) cytotoxicity assay has been applied on OEC (Oral epithelial cell) and Vero (Green monkey kidney) cell lines. Results revealed that 0.1% PBO concentration was safe and didn't induce any pathogenicity, as a dose concentration – dependent decreased pathological changes by comparing 0.3%, 0.2% PBO treated rats . Furthermore, SlNPV was safe to rat tissues and cells and PBO didn't alter the safe nature of SlNPV on mammalian cells. According to the results of histopthological bio-safety, 0.1% of PBO was applied to 4 th instar larvae for estimation its effect on larval enzyme activity and the results proved significant differences in the metabolic tested enzymes between the control and treated larvae with inhibition in both of Actylcholin esterase (AchE), Glutathione S-transferase (GST), Alpha esterases (α-esterases), Beta esterases (Besterases), and Glutamic oxaloacetic transaminase (GOT) and stimulation in Lactate dehydrogenase (LDH), Glutamic pyruvic transaminase (GPT) and Multi-function oxidases (MFO). Moreover, 0.1% of PBO has been mixed with viral LC50 for 2 nd and 4 th instar larvae and the results showed highly synergistic effect of PBO as 100 and 96 % mortality rate respectively fourth day","PeriodicalId":8506,"journal":{"name":"Asian Journal of Agriculture and Biology","volume":" ","pages":""},"PeriodicalIF":2.2,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42155872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}