A. Hussain, Sartaj Ali, Muhammad Muhammad, W. Akram, Syed Munir Hussain, Khalida Dawar
{"title":"Spatial distribution and risk associated with shot hole disease in apricot (Prunus armeniaca L.) in Northern Pakistan","authors":"A. Hussain, Sartaj Ali, Muhammad Muhammad, W. Akram, Syed Munir Hussain, Khalida Dawar","doi":"10.1080/03235408.2023.2203330","DOIUrl":"https://doi.org/10.1080/03235408.2023.2203330","url":null,"abstract":"Abstract Wilsonomyces carpophilus is the major fungus that produces shot hole disease (SHD) in apricots, which results in significant economic losses. This problem can be addressed through the monitoring of the diseases and spotlighting the risks connected with them. This study employed descriptive and geostatistical mapping techniques to track the spatial distribution and risk of SHD in apricot orchards in five apricot-growing valleys. The results showed a spatial distribution of the disease within the study area even varying within the valleys and orchards. A moderate spatial dependence of SHD prevalence (0.524), incidence (0.363), and severity index (0.313) were observed. GIS maps and trend analysis graphs also showed the spatial distribution of the disease from east-west and south-north directions. Climate and cultural practices favor the perpetuation of pathogens and the spread of SHD. This information will help apricot growers regarding disease management and improve sustainable profitability.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41246273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Lukman Ahamad, Z. Siddiqui, A. Hashem, E. F. Abd_Allah
{"title":"Use of AM fungus Rhizophagus irregularis and silicon dioxide nanoparticles for the management of Meloidogyne incognita, Alternaria dauci and Rhizoctonia solani and growth of carrot","authors":"Lukman Ahamad, Z. Siddiqui, A. Hashem, E. F. Abd_Allah","doi":"10.1080/03235408.2023.2207958","DOIUrl":"https://doi.org/10.1080/03235408.2023.2207958","url":null,"abstract":"Abstract Impacts of AM fungus Rhizophagus irregularis and silicon dioxide nanoparticles (SiO2NPs) were observed on the disease complex of carrot (Daucus carota) caused by Meloidogyne incognita, Alternaria dauci and Rhizoctonia solani. Use of R. irregularis alone together with SiO2NPs (0.10 mg ml−1) caused a significant increase in plant growth and photosynthetic pigments and reduce disease indices, galling and population of M. incognita. Use of R. irregularis resulted in a lesser plant growth and photosynthetic pigments than foliar spay of NPs. Use of R. irregularis with NPs spray was best for the management of disease complex. Leaf blight and root rot indices were 3 when fungal pathogen was inoculated alone and indices were 5 when pathogens were inoculated together. Use of R. irregularis/NPs with A. dauci/R. solani reduced indices to 2, indices were reduced to 1 when R. irregularis was used with SiO2 NPs. Root colonisation by R. irregularis was higher when alone but reduced in plants sprayed with NPs. Inoculation of test pathogens reduced colonisation by R. irregularis. Various studied attributes showed a significant correlation in principal components analysis. Significant management of disease complex may be achieved by the use of R. irregularis with SiO2 NPs.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41866486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Thapa, S. Mahapatra, D. Baral, A. Chatterjee, Pravesh Shivakoty, B. Subba, Srikanta Das
{"title":"Morpho-molecular variability of different isolates of Ustilagonoidea virens causing false smut of rice","authors":"S. Thapa, S. Mahapatra, D. Baral, A. Chatterjee, Pravesh Shivakoty, B. Subba, Srikanta Das","doi":"10.1080/03235408.2023.2208359","DOIUrl":"https://doi.org/10.1080/03235408.2023.2208359","url":null,"abstract":"Abstract Spore balls of false smut of rice caused by Ustilagonoidea virens were collected from different major rice belts of eastern part of India. Ten isolates from ten different locations were collected and isolated in the laboratory for their, cultural, morphological and molecular characterization. Potato sucrose agar media was used for their cultural studies and observation on colony diameter, colour and sporulation were made. Spores of all the isolates were observed under scanning electron microscope (SEM) and light microscope for spore size, spine length and colour. Differences in colony characters were observed where the highest mycelial growth was observed in isolate Uv2 with 5.33 cm colony diameter and lowest mycelial growth was observed in Uv9 (3.70 cm). Most of the isolates produced white and circular colony. Spores of all the isolates observed under a light microscope appeared to be olive green in colour, however, a significant variation in spore size and spike length under SEM was observed. Spores size and spike length of different isolates ranged between 4.533 μm to 6.169 μm and 362.567 nm to 799.533 nm, respectively. For genetic identification and variability, DNAs of isolates were amplified using ITS1 and ITS 4 primers. The length of nucleotide sequences of different isolates varied from 583 to 606 bp. Phylogenetic analysis revealed genetic variation among the isolates, dividing them into two major groups.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41726980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Identification and pathogenicity of Nigrospora sphaerica and Curvularia penniseti associated with leaf spot disease of cowpea (Vigna unguiculata (L.) Walp.) in Nigeria","authors":"I. Obisesan, P. Ojo","doi":"10.1080/03235408.2023.2195035","DOIUrl":"https://doi.org/10.1080/03235408.2023.2195035","url":null,"abstract":"Abstract Pathogenic fungi are one of the main causes of plant diseases due to their ability to colonise plants and rapidly spread disease. Cowpea is one of the most highly consumed grain legumes in Nigeria, but its yearly production is usually affected by fungi attacks. Disease presenting browning of the aerial parts of the cowpea plant was observed during the dry season between 2021 and 2022 in the country. This disease caused stunted growth in the affected plant stand. The fungi were isolated from the symptomatic aerial parts. The fungi were viewed under the microscope and identified by molecular and phylogenetic tools using the Internal Transcribed Spacer (ITS) gene region. The fungi isolated from the symptomatic aerial part of cowpea were identified as Nigrospora sphaerica and Culvularia penniseti. The pathogenicity of the isolated fungi was tested on detached leaves and stems and on intact cowpea seedlings. The test gave a positive result, which confirms the pathogenicity of the isolated fungi on the cowpea plant. This is the first report of N. sphaerica and C. penniseti infecting cowpea cultivated on farmland in Nigeria. Early detection of foreign fungi causing disease in the country’s cowpea crop will aid researchers in addressing the negative effects on crop yield.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48077749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Status of virus and phytoplasma diseases in carrot growing areas of Ankara and Konya provinces, Turkey","authors":"F. Randa-Zelyüt, F. Ertunç, D. Şenal","doi":"10.1080/03235408.2023.2191373","DOIUrl":"https://doi.org/10.1080/03235408.2023.2191373","url":null,"abstract":"Abstract In this study, infections that may emerge from viruses and phytoplasmas were investigated in carrot cultivation fields in the Ankara and Konya provinces, which are located in the Central Anatolia Region of Turkey and have the largest agricultural areas of the country. A total of 272 symptomatic and asymptomatic carrot plants, together with their root and leaf parts, were collected from 61 fields by performing periodic field studies during the plant vegetation periods between 2018–2019. Samples were tested against carrot virus Y (CarVY), carrot red leaf virus (CtRLV), carrot red leaf virus-associated RNA (CtRLVaRNA), carrot mottle virus (CMoV), carrot torradovirus 1 (CaTV), carrot yellow leaf virus (CYLV), carrot thin leaf virus (CTLV) and cucumber mosaic virus (CMV) agents by Reverse Transcriptase-PCR (RT-PCR). To determine phytoplasmas, samples were tested using universal primers by nested PCR. Molecular tests conducted to investigate the aforementioned viruses produced negative results, but phytoplasma infections were widely found. 16SrVI-A “Candidatus Phytoplasma trifolii” and 16SrI-B “Aster yellows” subgroups were detected by in vitro/in silico RFLP and sequencing analyses. Disease incidence caused by both phytoplasma groups was 39.34%. This study determined for the first time that 16SrVI and 16SrI groups cause severe infections in the carrot fields of Turkey.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44116386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abdul Razzaq, Arfan Ali, Sara Zahid, Arif Malik, L. Pengtao, Wànkuí Gǒng, Yuan Youlu, S. Ercişli, Muhammad Bilawal Junaid, M. Zafar
{"title":"Engineering of cry genes “Cry11 and Cry1h” in cotton (Gossypium hirsutum L.) for protection against insect pest attack","authors":"Abdul Razzaq, Arfan Ali, Sara Zahid, Arif Malik, L. Pengtao, Wànkuí Gǒng, Yuan Youlu, S. Ercişli, Muhammad Bilawal Junaid, M. Zafar","doi":"10.1080/03235408.2023.2192901","DOIUrl":"https://doi.org/10.1080/03235408.2023.2192901","url":null,"abstract":"Abstract Cotton is white gold and contributes significantly to the economy of the countries. The process of evolution of resistance in various cotton insects directly affects the life of people. Among the cotton insect pests, pink bollworm (Pectinophora gossypiella) and army bollworm (Spodoptera litura) have turned out to be destructive. The development of seed using gene stacking approach has tremendously reduced the applications of agrochemicals making the technology greener for society. A gene cassette containing Cry11 and Cry1H genes was constructed. The cassette was cloned into the pCAMBIA2300 plant expression vector using the CAMV3-35S promoter. The construct was transformed into cotton line FBS-222 using the shoot apex-cut Agrobacterium-mediated transformation method. The expression of the Cry genes was 3-fold higher in transgenic than the non-transgenic cotton plants using qRT-PCR. Finally, the insect bioassay with transgenic cotton showed 90% mortality against pink bollworms and 80% against cotton army bollworms. The results demonstrate the development of seeds using the gene pyramiding approach is an effective strategy to control insect pest attacks.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48576196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E. Evallo, J. W. Taguiam, I. B. Posada, M. Balendres
{"title":"Two additional Colletotrichum species causing leaf spot of rambutan (Nephelium lappaceum)","authors":"E. Evallo, J. W. Taguiam, I. B. Posada, M. Balendres","doi":"10.1080/03235408.2023.2188993","DOIUrl":"https://doi.org/10.1080/03235408.2023.2188993","url":null,"abstract":"Abstract Rambutan (Nephelium lappaceum) is an important fruit tree. Recently, leaf spots were observed in a tree in Los Baños, Laguna, Philippines. This study aims to identify the causal agent of rambutan leaf spot in the Philippines. Two fungal isolates, MBRAM001B and MBRAM003B, were isolated from the diseased leaves on potato dextrose agar medium. Colony growth and conidia characteristics of both isolates showed high resemblance with Colletotrichum species in the C. gloeosporioides species complex. Molecular characterisation of the isolates using the partial internal transcribed spacer rDNA and TUB2 regions identified the pathogens as C. tropicale MBRAM001B and C. siamense MBRAM003B. Leaf spots developed on rambutan leaves three days after inoculation and the same pathogens were isolated from the diseased leaves, establishing Koch’s Postulate. In conclusion, this study identified C. tropicale MBRAM001B and C. siamense MBRAM003B as causal agents of leaf spots in rambutan species in the Philippines. The survival and possible infection of these two Colletotrichum species on rambutan fruits and other fruit trees are worth further investigating.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44520478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Halima Maina Abdullahi, Muhammed Lawan, Duna Madu Mailafiya
{"title":"Silica nanoparticles and variety: their efficacy and interaction on the tomato fruit worm ( Helicoverpa armigera hubner) population infesting tomato (Solanum lycopersicum L.)","authors":"Halima Maina Abdullahi, Muhammed Lawan, Duna Madu Mailafiya","doi":"10.1080/03235408.2023.2185960","DOIUrl":"https://doi.org/10.1080/03235408.2023.2185960","url":null,"abstract":"Abstract Field experiments were conducted at the Teaching and Research Farms of the University of Maiduguri and Mohamet Lawan College of Agriculture, Maiduguri, Nigeria to determine the effects of silica nanoparticles and tomato variety on the tomato fruit worm (Helicoverpa armigera Hübner) (Lepidoptera: Noctuidae). The treatments each replicated thrice were factorially combined and laid out in a split-plot design with four tomato varieties namely: UC-82, Dan Syria, Roma VF and Tima and three concentrations of amorphous silica nanoparticles (1000 ppm, 2000 ppm and 3000 ppm) and cypermethrin (as a check). Insecticides were applied during the fruiting stage at 7 days interval for 8 weeks. Larval holes and marketable fruit yield were recorded. Silica nanoparticles at 3000 ppm resulted in fewer larval population (39.25) at p < 0.05 with a resultant decrease in number of damaged fruits (36.58) as compared to lower doses of 1000 ppm and 2000 ppm. Cypermethrin also gave minimum population of larvae (39.12). In all tested varieties, Roma VF had the lowest larval population (28.79). Variety Roma VF in combination with both cypermethrin and silica nanoparticle at 3000 ppm resulted in lower larval population (19.67) and (28.67) respectively. These results show that silica nanoparticle at 3000 ppm acting together with Roma VF appreciably controls H. armigera in tomato, making both practices important components of IPM against this larval pest.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42839273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Screening of soybean [Glycine max (L.) merrill] lines for reaction to natural field infection and resistant against bacteria foliar diseases","authors":"C. Afolabi, S. Salihu, O. Shokalu","doi":"10.1080/03235408.2023.2183796","DOIUrl":"https://doi.org/10.1080/03235408.2023.2183796","url":null,"abstract":"Abstract The production of Soybean in Nigeria is still inadequate due to the menace of pests and diseases. The current study was conducted to screen the performance of nineteen newly developed soybean lines that were infected naturally with bacterial diseases, identify promising line(s) with high yield potential and resistance against the diseases. Symptoms of soybean foliar diseases were accessed from the 4-12 week after planting, the resistance level of selected lines was also determined and bacteria isolate were culture and identify through morphological and biochemical tests. Four Bacteria species (Pseudomonas syringae, Xanthomonas spp, Clavibacter michiganensis and Bacillus subtilis) were isolated and identified from disease soybean leaf in this study. Whereas based on the pathogenicity test only two were pathogenic and cause soybean foliar disease (P. syringae and X. spp). Identified soybean lines differed significantly [p ≤ 0.05] in disease incidence and severity for both diseases. TGx 1990-57F, TGx 1990-55F were moderately resistant to bacterial blight and bacterial pustules. The study confirms that bacteria blight and pustule were present in the study area. It is therefore, recommended that the lines identified as moderately resistant to both diseases may help in future studies and evaluation in local hotspots.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41507088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Janki N. Thakker, Jinal Badrakia, Keyur B. Patel, Ujawala Makwana, Kinjal J. Parmar, P. Dhandhukia
{"title":"Potential of a marine Pseudomonas aeruginosa strain OG101 to combat Fusarium oxysporum associated wilt in legume crops","authors":"Janki N. Thakker, Jinal Badrakia, Keyur B. Patel, Ujawala Makwana, Kinjal J. Parmar, P. Dhandhukia","doi":"10.1080/03235408.2023.2183800","DOIUrl":"https://doi.org/10.1080/03235408.2023.2183800","url":null,"abstract":"Abstract Present study focuses on the use of bacterial isolate Pseudomonas OG101 against the Fusarium sp. and its growth promoting ability for chickpea (Cicer arietinum) and cowpea (Vigna unguiculata) plants. Pseudomonas strain OG101 controls mycelial growth of Fusarium oxysporum f.sp. ciceris and F. oxysporum f.sp. pallidoroseum up to 24.4% and 20.5%, respectively. In addition, OG101 showed a significant improvement in the germination index of 93.3% and 98.3% with disease index of 1.6% and 3.3% in chickpea plant and cowpea plant, respectively. Pot experiments of chickpea and cowpea plants refer to use it as a seed bacterization for the best effect for growth promotion and its biocontrol activity against F. oxysporum f.sp. ciceris and F. oxysporum f.sp. pallidoroseum respectively.","PeriodicalId":8323,"journal":{"name":"Archives of Phytopathology and Plant Protection","volume":null,"pages":null},"PeriodicalIF":1.0,"publicationDate":"2023-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44275299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}