Micro-farming specific morpho-molecular characterization of Trichoderma isolates and its bio-control potential against foliar fungal plant pathogens

IF 1 Q3 PLANT SCIENCES

Archives of Phytopathology and Plant Protection Pub Date : 2022-11-26 DOI:10.1080/03235408.2022.2164166

M. Chaithra, N. Pankaja, P. S. Benherlal, H. Mahesh

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Abstract

Abstract In order to understand the use of micro farming specific local bio-control agents against the foliar pathogens, twenty-one Trichoderma isolates were collected from different cropping locations of Karnataka. In morphological characterization: all colonies were appeared dark green with flat, even/uneven growth with 2–4 concentric rings and sporulation was observed after 2–4 days after inoculation on potato dextrose agar medium. The length × width of conidia and phialides varied from 2.5–4.6 μm × 2.5–4.6 μm and 6–11.3 μm × 2–3.5 μm respectively. In Basic Local Alignment Search Tool analysis of 5.8S-ITS region was amplified using ITS-4 and 5. These results assisted in species identification and classified into five clades (T. harzianum: 11, T. asperellum; 5, T. virde; 2, T. virens; 2 and T. longibrachiatum; 1). Further, in antagonistic activity, RMlO exhibited highest growth inhibition of Asperisporium caricae (96.30%), Pyricularia oryzae (97.03%) and Bipolaris oryzae (100%)) significantly over the commercial isolate (A. caricae (85.93%); P. oryzae (89.38%) and B. oryzae (91.92%)).

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木霉分离株的微农业特异性形态分子特征及其对叶片真菌植物病原体的生物防治潜力

摘要为了解微农业局部生物防治剂对病原菌的防治作用，从卡纳塔克邦不同种植地点采集了21株木霉分离株。形态特征:菌落呈墨绿色，生长平坦，均匀/不均匀，有2-4个同心圆环，在马铃薯葡萄糖琼脂培养基上接种2-4天后开始产孢。分生孢子的长×宽分别为2.5 ~ 4.6 μm × 2.5 ~ 4.6 μm和6 ~ 11.3 μm × 2 ~ 3.5 μm。在Basic Local Alignment Search Tool中，利用ITS-4和its - 5对5.8S-ITS区域进行扩增。这些结果有助于物种鉴定，并将其划分为五个支系(哈氏霉属:11支，曲霉属;5、T. virde;2、T. virens;2、长尾叶桐;1)此外，在拮抗活性方面，RMlO对稻曲霉(Asperisporium caricae)、稻瘟病菌(pyraria oryzae)和稻双极菌(Bipolaris oryzae)的抑制作用最高(96.30%)，显著高于商品分离菌(85.93%);P. oryzae(89.38%)和B. oryzae(91.92%))。

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来源期刊

Archives of Phytopathology and Plant Protection Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

2.20

自引率

0.00%

发文量

100

期刊介绍： Archives of Phytopathology and Plant Protection publishes original papers and reviews covering all scientific aspects of modern plant protection. Subjects include phytopathological virology, bacteriology, mycology, herbal studies and applied nematology and entomology as well as strategies and tactics of protecting crop plants and stocks of crop products against diseases. The journal provides a permanent forum for discussion of questions relating to the influence of plant protection measures on soil, water and air quality and on the fauna and flora, as well as to their interdependence in ecosystems of cultivated and neighbouring areas.