The Behavior analyst最新文献

A dual-response NIR fluorescent probe for separately and continuously recognizing H2S and Cys with different fluorescence signals and its applications. 一种分别连续识别不同荧光信号H2S和Cys的双响应近红外荧光探针及其应用

The Behavior analyst Pub Date : 2023-08-25 DOI: 10.2139/ssrn.4331007

Lisha Yue, Yin Ai, Gang Liu, Haichang Ding, S. Pu

引用次数: 0

From droplets to ions: a comprehensive and consecutive ion formation modelling in atmosphere pressure interface of electrospray ionization mass spectrometry. 从液滴到离子:电喷雾电离质谱法大气压界面中全面连续的离子形成模型。

The Behavior analyst Pub Date : 2023-06-20 DOI: 10.2139/ssrn.4397243

He Xingliang, Guo Xing, Wu Mengfan, Deng Fulong, Zeng Pengyu, Z. Zhongjun, Duan Yixiang

引用次数: 0

Monitoring bacterial spore metabolic activity using heavy water-induced Raman peak evolution. 利用重水诱导的拉曼峰进化监测细菌孢子代谢活性。

The Behavior analyst Pub Date : 2023-04-11 DOI: 10.2139/ssrn.4292992

Rasmus Öberg, Tobias Dahlberg, Dmitry Malyshev, Magnus Andersson

{"title":"Monitoring bacterial spore metabolic activity using heavy water-induced Raman peak evolution.","authors":"Rasmus Öberg, Tobias Dahlberg, Dmitry Malyshev, Magnus Andersson","doi":"10.2139/ssrn.4292992","DOIUrl":"https://doi.org/10.2139/ssrn.4292992","url":null,"abstract":"Endospore-forming bacteria are associated with food spoilage, food poisoning, and infection in hospitals. Therefore, methods to monitor spore metabolic activity and verify sterilization are of great interest. However, current methods for tracking metabolic activity are time-consuming and resource intensive. This work investigates isotope labeling and Raman microscopy as a low-cost rapid alternative. Specifically, we monitor the Raman spectrum of enterotoxic B. cereus spores undergoing germination and cell division in D2O-infused broth. During germination and cell division, water is metabolized and deuterium from the broth is incorporated into proteins and lipids, resulting in the appearance of a Raman peak related to C-D bonds at 2190 cm-1. We find that a significant C-D peak appears after 2 h of incubation at 37 °C. Further, we found that the peak appearance coincides with the observed first cell division indicating little metabolic activity during germination. Lastly, the germination and cell growth rate of spores were not affected by adding 30% heavy water to the broth. This shows the potential for real-time monitoring of metabolic activity from a bacterial spore to a dividing cell. In conclusion, our work proposes tracking the evolution of the C-D Raman peak in spores incubated with D2O-infused broth as an effective and time-, and cost-efficient method to monitor the outgrowth of a spore population, simultaneously allowing us to track for how long the bacteria have grown and divided.","PeriodicalId":82956,"journal":{"name":"The Behavior analyst","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84605017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Development of a mid-infrared sensor system for early fire identification in cotton harvesting operations. 用于棉花收获作业早期火灾识别的中红外传感器系统的研制。

The Behavior analyst Pub Date : 2022-11-29 DOI: 10.2139/ssrn.4167287

Yafei Li, Yang Lu, Chuantao Zheng, Shuo Yang, Kaiyuan Zheng, Fang Song, Chunguang Li, W. Ye, Yu Zhang, Yiding Wang, F. Tittel

引用次数: 1

An electrochemical sensor based on AuPd@FexOy nanozymes for a sensitive and in situ quantitative detection of hydrogen peroxide in real samples. 一种基于AuPd@FexOy纳米酶的电化学传感器，用于实际样品中过氧化氢的敏感和原位定量检测。

The Behavior analyst Pub Date : 2022-11-21 DOI: 10.2139/ssrn.4165889

Mengjiao Dai, Qunyan Zhu, Dongxue Han, L. Niu, Zhenxin Wang

{"title":"An electrochemical sensor based on AuPd@FexOy nanozymes for a sensitive and in situ quantitative detection of hydrogen peroxide in real samples.","authors":"Mengjiao Dai, Qunyan Zhu, Dongxue Han, L. Niu, Zhenxin Wang","doi":"10.2139/ssrn.4165889","DOIUrl":"https://doi.org/10.2139/ssrn.4165889","url":null,"abstract":"The hydrogen peroxide (H2O2) levels in living organisms and environment have strong effects on many biological processes inducing cell apoptosis/cell necrosis and wound disinfection. Therefore, it is important to have an accurate and in situ detection of H2O2. Herein, an AuPd@FexOy nanozyme-based electrochemical (EC) sensor (termed as AuPd@FexOy NPs/GCE) with good stability and anti-interference ability has been prepared for the detection of H2O2 by differential pulse voltammetry (DPV) and chronoamperometry dual-measurement modes. The AuPd@FexOy NPs/GCE exhibits good linear relationships in the ranges from 13.0 to 6.0 × 103 μM (DPV measurement) and 50 to 1.0 × 103 μM (chronoamperometry measurement), low detection limits (LODs) of 1.6 μM (DPV measurement) and 3.0 μM (chronoamperometry measurement) and high sensitivities of 83.8 nA μM-1 cm-2 (DPV measurement) and 120.7 nA μM-1 cm-2 (chronoamperometry measurement). The practicability of the as-prepared AuPd@FexOy NPs/GCE has been demonstrated by an in situ real-time detection of H2O2 released from adherent living MCF-7 cells triggered by varying amounts of N-formyl-L-methionyl-L-leucyl-L-phenylalanine (FMLP) from 0.5 to 3.0 μM and the quantitative determination of H2O2 in commercial disinfectants.","PeriodicalId":82956,"journal":{"name":"The Behavior analyst","volume":"2002 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78554022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Signal amplification surface-enhanced Raman scattering immunosorbent assay of human chorionic gonadotrophin based on repeated enzyme biocatalytic precipitation. 基于重复酶生物催化沉淀的人绒毛膜促性腺激素的信号放大表面增强拉曼散射免疫吸附测定。

The Behavior analyst Pub Date : 2022-11-14 DOI: 10.2139/ssrn.4197044

Cuicui Fu, Li Zhang, Meiqi Bao, Yue Zhang, Ya-li Li, Yan Wu, Young Mee Jung

{"title":"Signal amplification surface-enhanced Raman scattering immunosorbent assay of human chorionic gonadotrophin based on repeated enzyme biocatalytic precipitation.","authors":"Cuicui Fu, Li Zhang, Meiqi Bao, Yue Zhang, Ya-li Li, Yan Wu, Young Mee Jung","doi":"10.2139/ssrn.4197044","DOIUrl":"https://doi.org/10.2139/ssrn.4197044","url":null,"abstract":"A novel surface-enhanced Raman scattering (SERS) immunoassay method based on tyramine signal amplification (TSA) technology triggering the formation of enzyme repeats on an enzyme-linked immunosorbent assay (ELISA) was designed for highly sensitive detection of human chorionic gonadotropin (hCG) using enzymatic biocatalytic precipitation toward o-phenylenediamine (OPD). Initially, a horseradish peroxidase (HRP)-labeled hCG antibody was fixed by the double antibody sandwich method, and then a tyramine-HRP conjugate was used to form HRP repeats by triggering the immobilized HRP on ELISA with the aid of H2O2. In the presence of the target hCG, the HRP repeats carried by the sandwich immune complex catalyzed the oxidation of OPD to produce product molecules with different structures, resulting in changes in the SERS fingerprint spectrum. The analytical performance of the SERS immunoassay was studied in detail using SERS spectral characterization. Under the optimum conditions, the immunosensor displayed a working range from 1 IU L-1 to 16 IU L-1 with a detection limit (LOD) of 0.17 IU L-1 relative to the target hCG. Compared to the traditional SERS immunosensor, a higher detection sensitivity can be obtained. Therefore, this work provides a new strategy for hCG detection and inspiration for the construction of sensitive and efficient immunosensors.","PeriodicalId":82956,"journal":{"name":"The Behavior analyst","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91174063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Simple ultrasensitive electrochemical detection of the DBP plasticizer for the risk assessment of South Korean river waters. 韩国河流水体中DBP增塑剂的简单超灵敏电化学检测风险评估。

The Behavior analyst Pub Date : 2022-07-05 DOI: 10.2139/ssrn.4069170

N. G. Gurudatt, Kyungyeon Lee, W. Heo, Hyo-Il Jung

{"title":"Simple ultrasensitive electrochemical detection of the DBP plasticizer for the risk assessment of South Korean river waters.","authors":"N. G. Gurudatt, Kyungyeon Lee, W. Heo, Hyo-Il Jung","doi":"10.2139/ssrn.4069170","DOIUrl":"https://doi.org/10.2139/ssrn.4069170","url":null,"abstract":"Rapid detection of contaminants for the purpose of sensitive and quantitative monitoring of environmental hazards is an essential first step in realizing the avoidance of human health risks. In this regard, we present a fast and simple electrochemical method of detecting di-n-butyl phthalate (DBP) from river water samples using a phthalic acid group specific aptamer modified on a gold nanoparticle (AuNP) functionalized graphene oxide nano-platelet (GO) and ionic liquid (IL) nanocomposite. Here, the IL/GO nanocomposite allows an enhanced interaction with phthalate esters, thereby increasing the sensitivity of the sensor surface. The proposed sensor showed a wide linear dynamic range from 0.14 pg mL-1 to 0.35 ng mL-1 and from 0.35 ng mL-1 to 7 ng mL-1 with a detection limit of ≤0.042 pg mL-1, which were evaluated using standard, analytical grade DBP; the limit of quantification was determined using different concentrations of DBP in DI water in comparison with gas chromatography-mass spectroscopy (GC/MS) values. The proposed sensor was used to monitor the DBP concentrations in river water samples collected from various locations across South Korea. The quantitative data from the measurements in comparison with standard GC/MS values were then used to ascertain the human health risk posed by the daily consumption of these river waters.","PeriodicalId":82956,"journal":{"name":"The Behavior analyst","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83261140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

A facile visualized solid-phase detection of virus-specific nucleic acid sequences through an upconversion activated linear luminescence recovery process. 通过上转换激活的线性发光恢复过程，对病毒特异性核酸序列进行简单的可视化固相检测。

The Behavior analyst Pub Date : 2022-05-11 DOI: 10.2139/ssrn.4015241

Xiaorong Liu, Chaonan He, Qi Huang, Mengmeng Yu, Zhuang Qiu, Haoxin Cheng, Yifei Yang, Xian Hao, Xiaolei Wang

{"title":"A facile visualized solid-phase detection of virus-specific nucleic acid sequences through an upconversion activated linear luminescence recovery process.","authors":"Xiaorong Liu, Chaonan He, Qi Huang, Mengmeng Yu, Zhuang Qiu, Haoxin Cheng, Yifei Yang, Xian Hao, Xiaolei Wang","doi":"10.2139/ssrn.4015241","DOIUrl":"https://doi.org/10.2139/ssrn.4015241","url":null,"abstract":"The development of portable solid-phase biosensors is of great significance for point-of-care testing (POCT). In this work, we constructed a simple visualized solid-phase biosensor based on luminescence resonance energy transfer (LRET) from upconversion nanoparticles (UCNPs) to gold nanoparticles (AuNPs) for quantitative detection of virus-specific nucleic acid sequences. The detection data showed that there was a linear relationship between the luminescence recovery of UCNPs and the concentrations of the target within the range of 5-100 nM, and the limit of detection (LOD) was 0.326 nM. Additionally, the luminescence recovery of UCNPs was visualized and quantitatively analyzed using a home-built luminescence image capture device and an open-source ImageJ software that can analyze and process images. Compared with conventional liquid-phase biosensors, the solid-phase analysis method we constructed not only has advantages in cost, portability and stability, but also is more conducive to the rapid acquisition and storage of the detected sample, which is expected to become a fast, efficient and reliable detection platform for POCT.","PeriodicalId":82956,"journal":{"name":"The Behavior analyst","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88066556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Fabrication of functional group-rich monoliths for magnetic field-assisted in-tube solid phase microextraction of inorganic selenium species in water samples followed by online chromatographic determination. 富官能团单块的制备及其对水样中无机硒的固相微萃取及在线色谱测定。

The Behavior analyst Pub Date : 2022-03-15 DOI: 10.2139/ssrn.3946284

Xiaochong Song, Siyu Luo, Jun Liu, Yuanfei Wu, Xiaojia Huang

{"title":"Fabrication of functional group-rich monoliths for magnetic field-assisted in-tube solid phase microextraction of inorganic selenium species in water samples followed by online chromatographic determination.","authors":"Xiaochong Song, Siyu Luo, Jun Liu, Yuanfei Wu, Xiaojia Huang","doi":"10.2139/ssrn.3946284","DOIUrl":"https://doi.org/10.2139/ssrn.3946284","url":null,"abstract":"Efficient separation and enrichment is a crucial step in the analysis of Se(IV) and Se(VI). In the present study, for the first time, online monolith-based magnetic field-assisted in-tube solid phase microextraction (MFA/IT-SPME) was applied to capture inorganic selenium species in water samples. To this aim, porous monoliths mixed with magnetic nanoparticles were synthesized in a silica capillary and employed as a microextraction column (MEC) for MFA/IT-SPME. After that, a magnetic coil utilized to induce variable magnetic fields in adsorption and desorption steps was entwined around the MEC. Se(IV) was coordinated with o-phenylenediamine to form a coordination compound that was infused onto the MEC to be captured. Results evidenced that application of magnetic field during the extraction procedure assisted the capture of the Se(IV)-OPA complex, with an enhancement in the extraction efficiency from 83% to 97%. Under the optimized conditions, MFA/IT-SPME was online combined with HPLC equipped with a diode array detector (DAD) to perform quantification of Se(IV) and Se(VI) in environmental water samples. Total inorganic Se was quantified after pre-reduction of Se(VI) to Se(IV) prior to applying the established approach, and a subtraction method was adopted to calculate the Se(VI) and Se(IV) contents. The limit of detection for Se(IV) was as low as 0.012 μg L-1. The reliability of the suggested method was investigated by assaying Se(IV) and Se(VI) species in real-life water samples with satisfactory recoveries (81.1%-116%) and repeatability (RSDs below 9%).","PeriodicalId":82956,"journal":{"name":"The Behavior analyst","volume":"86 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83971194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

A headspace collection chamber for whole body volatilomics. 一个收集全身挥发物的顶空室。

The Behavior analyst Pub Date : 2022-02-10 DOI: 10.26434/chemrxiv-2022-rmf6x

Stephanie Rankin-Turner, C. McMeniman

{"title":"A headspace collection chamber for whole body volatilomics.","authors":"Stephanie Rankin-Turner, C. McMeniman","doi":"10.26434/chemrxiv-2022-rmf6x","DOIUrl":"https://doi.org/10.26434/chemrxiv-2022-rmf6x","url":null,"abstract":"The human body secretes a complex blend of volatile organic compounds (VOCs) via the skin, breath and bodily fluids, the study of which can provide valuable insight into the physiological and metabolic state of an individual. Methods to profile human-derived volatiles typically source VOCs from bodily fluids, exhaled breath or skin of isolated body parts. To facilitate profiling the whole body volatilome, we have engineered a sampling chamber that enables the collection and analysis of headspace from the entire human body. Whole body VOCs were collected from a cohort of 20 humans and analyzed by thermal desorption-gas chromatography/mass spectrometry (TD-GC/MS) to characterize the compounds present in whole body headspace and evaluate chemical differences between individuals. A range of compounds were detected and identified in whole body headspace including ketones, carboxylic acids, aldehydes, alcohols, and aliphatic and aromatic hydrocarbons. Considerable heterogeneity in the chemical composition of whole body odor and the concentration of its constituent compounds was observed across individuals. Amongst the most common and abundant compounds detected in human whole body odor were sulcatone, acetoin, acetic acid and C6-C10 aldehydes. This method facilitates standardized and quantitative analytical profiling of the human whole body volatilome.","PeriodicalId":82956,"journal":{"name":"The Behavior analyst","volume":"63 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80784460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4