{"title":"Temperature dependent pH-instability of some alpha-L-arabinofuranosidases.","authors":"G Butschak, A Küster, B Schulze, A Graffi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In this paper are described alpha-L-arabinofuranosidases, partially purified from some moulds, with very high lability of their activity to weakly alkaline or neutral pH-values. This pH-lability strongly depends on temperature. The form of the inactivation curves suggests involvement of charge changes in this irreversible process. These enzymes may be useful for optimization of the tumor therapy concept described by Graffi (5).</p>","PeriodicalId":8274,"journal":{"name":"Archiv fur Geschwulstforschung","volume":"59 3","pages":"165-70"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13897268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Prognostic factors in laryngeal cancer].","authors":"B Kramp, U Sievert","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A retrospective analysis of 335 patients with laryngeal and hypopharyngeal cancer from the ENT-Hospital of the Wilhelm-Pieck-University Rostock, who died to December 1986, was made. Adjusted five-year survival rates were in glottic carcinoma 54.2 per cent and supraglottic carcinoma 42.9 per cent. This is significantly higher than in carcinoma of hypopharynx with 15.4 per cent. The following prognostic factors were important for the survival rates of the patients without consideration of the therapy of primary tumor: pTNM-stage, evidence and development of regional metastasis, enlarged stage of the primary tumor at the time of diagnosis; intercurrent diseases; development of distant metastasis and/or second carcinoma; localisation of tumor; primary therapy of regional lymph nodes.</p>","PeriodicalId":8274,"journal":{"name":"Archiv fur Geschwulstforschung","volume":"59 3","pages":"183-9"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13897272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Structure-activity relations of antineoplastic platinum II and platinum IV coordination compounds].","authors":"W Gutsche, J Baumgart, H P Schröer","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Five diammine-Pt(II) or Pt(IV) coordination compounds, namely cis-diammine-dichloro-platinum (II) \"cis-DDP\", transdihydroxy-cis-diammine-dichloro-platinum (IV) \"trans-ODDP\", and derived substitution products of lactic acid (racemates or L-forms) with diminished toxicity in comparison to cis-DDP have been tested against mouse leukemia P388, and partly on melanoma B16 for antineoplastic activity. The results have been compared with those obtained with the clinical approved cis-DDP. They were not in every way equal to the antitumor efficiency of cis-DDP. Improved physicochemical properties as well as favorable differences of side effects in contrast to cis-DDP, could be decisive for the potential value of these substances.</p>","PeriodicalId":8274,"journal":{"name":"Archiv fur Geschwulstforschung","volume":"59 4","pages":"233-8"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13940656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comprehensive cancer centres in the Federal Republic of Germany.","authors":"C G Schmidt","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":8274,"journal":{"name":"Archiv fur Geschwulstforschung","volume":"59 2","pages":"153-5"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13859256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cancer in Finland.","authors":"E A Saxén","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":8274,"journal":{"name":"Archiv fur Geschwulstforschung","volume":"59 2","pages":"157-8"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13859258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H Donat, L Bernstein, H Schenk, E Erbstösser, A Rosé
{"title":"[Determination of N-acetylneuraminic acid for the differential diagnosis of cystic ovarian tumors].","authors":"H Donat, L Bernstein, H Schenk, E Erbstösser, A Rosé","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The concentrations of N-acetylneuraminic acid (NANA) were determined in the fluid of cystic ovarian tumors and in the blood serum from a total of 95 patients. The estimation of NANA was based on the method of Svennerholm. It was found that the mean content of NANA in the cystic fluid is more than 3.0 mmol/l in serous and more than 5.5 mmol/l in mucinous malignant ovarian tumors. The mean value of NANA in the serum of patients is mostly more than 3.5 mmol/l if there is an ovarian cancer. The estimation of NANA is useful for the preoperative diagnosis of cystic ovarian tumors.</p>","PeriodicalId":8274,"journal":{"name":"Archiv fur Geschwulstforschung","volume":"59 2","pages":"113-20"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13861251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[NMR research on blood plasma for tumor detection--a method for mass screening?].","authors":"H Fritzsche, H Sprinz, H Jahn, D Jorke","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Recently, a simple NMR test was proposed to distinguish patients with and without malignant diseases by line-width analysis of methyl and methylene lipoprotein resonances in human plasma (4). We have reevaluated this proposed cancer detection technique at 250 MHz (magnet field strength 5.9T). The mean line width for 11 controls was 31.2 Hz (SD 4.1 Hz). For 8 patients with untreated malignant tumors, the line width was 27.5 Hz (SD 3.9 Hz). The data confirm the reported lower line widths of human plasma NMR resonances for patients with malignant tumors compared with controls (4). However, the statistical significance (P greater than 0.07) does not justify to use this technique for individual differential diagnostic purposes without further improvements of the method.</p>","PeriodicalId":8274,"journal":{"name":"Archiv fur Geschwulstforschung","volume":"59 2","pages":"129-33"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13861253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
E Jezewska, M K Górny, A Skrzypczak, A Słowik-Gabryelska, J Zeromski
{"title":"Assessment of phenotype of blood lymphocyte subsets prior and after PWM stimulation in patients with lung carcinoma.","authors":"E Jezewska, M K Górny, A Skrzypczak, A Słowik-Gabryelska, J Zeromski","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Surface phenotypes of peripheral blood lymphocytes of lung cancer patients and those of two control groups were assessed by means of indirect immunofluorescence with monoclonal antibodies, prior and after 10 day pokeweed mitogen (PWM) in vitro stimulation. There was no significant alteration in pan T cell per cent values prior and after mitogen stimulation in all groups tested. CD4+ cells in lung cancer group were however significantly decreased as compared to blood donor group (37.3% vs 44.9%, p less than 0.05). This decline was even more pronounced in III/IVo stage of tumour progression according to TNM classification (36.8%, p less than 0.05). These changes, however were not cancer specific, because similar decrease of CD4+ cells was seen in a group of patients with nonneoplastic lung conditions (35.7%, p less than 0.01). Following 10 day PWM culture per cent values of CD4+ cells did not change significantly. The assessment of CD8+ lymphocytes has shown marked differences in two subgroups of lung cancer, namely in II (17.4%) and III/IV (26.2%) of tumour progression (p less than 0.05), which returned to normal values following PWM culture. CD4/CD8 ratio was distinctly depressed in cancer patients in relation to donors. The evaluation of surface markers of B lymphocytes activated cells and monocytes did not show significant alterations in all groups examined. Per cent of HNK1+ cells was heightened in cancer group, especially in III/IV stage of tumour progression in relation to donors (21.7% and 22.8% vs 17.3%, p less than 0.05 respectively). PWM stimulation resulted in marked fall of HNK1+ cells to values corresponding to those in donor group. This study indicates some alterations in per cent values of blood lymphocytes subpopulations belonging mainly to T cell lineage in lung cancer patients linked to tumour staging which only partially return to normal following PWM stimulation.</p>","PeriodicalId":8274,"journal":{"name":"Archiv fur Geschwulstforschung","volume":"59 1","pages":"31-6"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14058625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Psychosocial aspects and rehabilitation problems of patients with tumors of the ORL region with special reference to laryngectomees].","authors":"E Werner, K Berger, H P Heilmann","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The operational therapy of malign tumours in the ORL is often connected with visible larger tissue organ lesions respectively. By this reason intensive efforts regarding comprehensive rehabilitation are necessary with the aim of overcoming the multiple impairments of familiar, social and occupational life. It is demonstrated with making inquiries concerning tight cooperation between patients, physicians, relatives, speech therapists and rehabilitation centres by example of laryngectomees as a premise for an successful reincorporation into a normal life.</p>","PeriodicalId":8274,"journal":{"name":"Archiv fur Geschwulstforschung","volume":"59 1","pages":"19-22"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14058893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cytophotometric and interference microscopic investigations in carcinomas of the oral cavity.","authors":"G Taubert, R Dammer, H Krug, R Graul","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The DNA content and the nuclear dry mass of 18 keratinized squamous cell carcinomas and their metastases, of one adamantinoma recurrence and two adenoid cystic carcinomas of the oral cavity were determined in comparison to the normal buccal mucosa using Feulgen scanning cytophotometry and interference microscopy. The squamous cell carcinomas could be classified into five groups based on their DNA distribution pattern. The nuclear dry mass and its variation were found to be different from the normal mucosal epithelial cells in all cases. No differences could be found between diploid cells and cells of a higher degree of ploidy. Therefore the relation of nuclear dry mass and DNA content appeared to be lower in cells of higher DNA ploidy. This fact is discussed in relation to the underlying molecular biological processes. This leads to the conclusion that the increase of the nuclear dry mass (mainly protein) precedes the increase of the DNA content. The combined measurement of DNA content and nuclear dry mass allows a better characterization of malignancy than each of the nuclear components measured alone. The difference of nuclear dry mass between malignant cells and normal mucosal epithelial cells is more sensitive than the deviation of the DNA content owing to the fact that malignant tumours with normal DNA content exist.</p>","PeriodicalId":8274,"journal":{"name":"Archiv fur Geschwulstforschung","volume":"59 5","pages":"353-60"},"PeriodicalIF":0.0,"publicationDate":"1989-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13732821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}