Archiv fur Geschwulstforschung最新文献

{"title":"[Sialic acid in the serum--an indicator for malignant growth?].","authors":"G Schulze","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Changes of the sialic acid content of patients sera reflect growth processes of benign and malignant character. Using an enzymatic test the value of sialic acid as a primary diagnostic parameter has been investigated. We found a poor diagnostic sensitivity with exception of lung and ovarian carcinomas where the sensitivity attained nearly 90%. In monitoring of patients with pre-therapeutically elevated sialic acid content in serum dropping values indicate a successful therapeutic intervention. An increase of post-therapeutic values demonstrates recurrent processes but the reaction of the serum level may be delayed up to 2 weeks. The outcome of the studies supports the notion that sialic acid is a tumor marker of very limited value.</p>","PeriodicalId":8274,"journal":{"name":"Archiv fur Geschwulstforschung","volume":"60 1","pages":"19-24"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13460555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Distribution studies of alpha-L-arabinofuranosidase already unstable at pH 7.0 (37 degrees C) in tumor-bearing mice in connection with its possible use to enhance the selectivity of the chemotherapy of malignant tumors].","authors":"G Butschak,&nbsp;B Schulze,&nbsp;A Küster,&nbsp;T Niederhausen,&nbsp;U Niemeyer,&nbsp;A Graffi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Graffi et al. (1-3) had proposed the use of exogenous enzymes to toxify inactive transport forms of cancerostatic substances. For this purpose, the pH difference between normal tissues and the tumor was to be exploited, which can be essentially increased by the application of glucose and inorganic phosphate (5-7). Earlier studies using alpha-L-arabinofuranosidase obtained from Aspergillus niger have shown that the selectivity of tumor chemotherapy can be increased in this way (4). The alpha-L-arabinofuranosidases known to date are stabile in a wide pH range (9). However, in some moulds we found pH-labile enzymes of this kind that become irreversibly inactivated in the weakly alkaline or neutral pH range (10, 11). Studies on the distribution of the activity of a pH-labile alpha-L-arabinofuranosidase from Glomerella myabana in tumor-bearing mice have shown that this enzyme is rapidly eliminated from the organism, in contrast to the pH-stable alpha-L-arabinofuranosidase from A. niger. Apart from its excretion via kidney and liver, of importance is the inactivation of the enzyme in the normal tissues. The additional application of glucose strongly increased the activity of this enzyme both in the tumor and in normal tissues (12). By injecting alkaline solutions, stronger inactivation in normal tissues than in the tumor was achieved (13). In the present paper, distribution of an alpha-L-arabinofuranosidase from Fusarium species I 50 (11), inactive already at pH 7.0 (37 degrees C), was studied in tumor-bearing mice. The activity of this enzyme could be enriched under various conditions in the tumor, and especially favorable proved to be the additional application of a combination of glucose and inorganic phosphate. Under these conditions, a higher activity than in the tumor was demonstrable only in the kidney, which can possibly be eliminated in larger experimental animals by diuretics or an appropriate alkaline administration. The investigations have shown that the pH-labile alpha-L-arabinofuranosidases, especially those of Fusarium sp., due to their pharmacokinetic behavior are better suited for use in our therapy concept than the hitherto employed enzyme from A. niger. More recently, Tietze (16) has proposed a similar therapy concept, in which also the glucose-increased pH difference between tumor and normal tissue using tumor-own enzymes, exogenous enzymes as well as transport forms of cancerostatic agents spontaneously hydrolysing under weakly acidic pH conditions is to be exploited.</p>","PeriodicalId":8274,"journal":{"name":"Archiv fur Geschwulstforschung","volume":"60 3","pages":"193-200"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13517780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The usefulness of monthly breast self-examination for the early detection of breast cancer].","authors":"S Kloskowski,&nbsp;K Ebeling","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>At present, the influence of BSE on mortality reduction from breast cancer is not yet clear. Within a larger case-control study in 195 cases and 390 controls, BSE practice, factors influencing BSE-frequency and the impact of BSE on tumor size as well as on the number of positive lymph-nodes have been investigated. 44% of women practised BSE monthly. Factors significantly positively correlated with BSE were older age, higher education, prior breast biopsy, participation in cervical cancer screening and regular physical breast examination. In comparison to non-users, BSE users had a relative risk for breast cancer more than 3 cm in tumor size of 0.65 (0.35-1.21). The relative risk of BSE-users for breast cancer with more than 3 positive lymph-nodes at the time of diagnosis amounted to 0.62 (0.33-1.18).</p>","PeriodicalId":8274,"journal":{"name":"Archiv fur Geschwulstforschung","volume":"60 5","pages":"373-8"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13392813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Symposium \"Rational diagnosis in oncology\". 17-18 November 1988, Berlin-Buch].","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":8274,"journal":{"name":"Archiv fur Geschwulstforschung","volume":"60 1","pages":"35-70"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13460554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transfection by DNA-nuclear protein HMG1 complexes: raising of efficiency and role of DNA topology.","authors":"M Böttger,&nbsp;M Platzer,&nbsp;U Kiessling,&nbsp;M Strauss","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We have developed a novel and efficient transfection method based on the introduction of foreign DNA into mammalian cells in form of complexes of vector DNA with the nuclear protein HMG1. In this study, it is shown that a stabilization of the complexes against dilution dissociation by addition of soluble CaCl2 or by excessive HMG1 enhances the transfection efficiency. Furthermore, there are no differences in the transfection abilities between the 3 topological DNA forms, viz., supercoiled, open relaxed and linear DNA, if delivered to cells as HMG1-DNA complexes. It is further shown that transfection-inactive complexes of the core histones with foreign DNA can be activated in transfection by the addition of HMG1.</p>","PeriodicalId":8274,"journal":{"name":"Archiv fur Geschwulstforschung","volume":"60 4","pages":"265-70"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13537919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dietary fructose enhances the development of atypical acinar cell nodules in the pancreas of rats pretreated with N-nitrosomorpholine.","authors":"H Enzmann,&nbsp;T Dettler,&nbsp;D Ohlhauser,&nbsp;H Stumpf,&nbsp;P Bannasch","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Dietary influences on carcinogenesis have been shown in various tissues on the basis of epidemiological and experimental approaches. The present paper demonstrates that the oral application of fructose over long periods to rats previously treated with N-nitrosomorpholine increases the incidence of atypical acinar cell nodules (AACN) in the pancreas. Whereas an increase in AACN was statistically significant (P less than 0.005), no effect on ductule-like structures was detectable. AACN have been regarded as precursor lesions of the acinar cell carcinoma. Although the AACN observed frequently exhibited severe atypia and numerous mitotic figures, no clearcut evidence for malignancy could be detected under our experimental conditions.</p>","PeriodicalId":8274,"journal":{"name":"Archiv fur Geschwulstforschung","volume":"60 4","pages":"283-7"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13537922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Induction of leukemia in chicken by bovine leukemia virus due to insertional mutagenesis.","authors":"V Altanerova,&nbsp;J Ban,&nbsp;R Kettmann,&nbsp;C Altaner","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Bovine leukemia virus (BLV) was inoculated into one-day-old chickens. In a small part of inoculated chickens leukemia developed during observation period of one year. Out of 88 birds inoculated, only 4 developed histopathologically verified leukemia. The induced leukemia was characterized by enlarged liver and spleen. The organs were infiltrated with leukemic cells. The DNAs of body organs of inoculated chickens were analysed by Southern blot hybridization for the presence of BLV specific sequences. Out of 9 suspicious chickens tested in 6 birds the BLV was found to be integrated into host DNA either as a complete viral genome or as a part corresponding to its 3'-end. The leukemic cells were monoclonal as regard to the integration site of the BLV provirus. Neither the expression of BLV provirus in chicken leukemic cells nor the antibody response to BLV antigens in inoculated birds was detected. The rearrangements and amplification of erb-B and myb loci of protooncogenes in leukemic cells was detected. There were no changes in loci of following protooncogenes: myc, sis, fes, fps, erb A, src and yes. All obtained data taken together suggest that the BLV induced leukemia in chickens is caused by insertional mutagenesis.</p>","PeriodicalId":8274,"journal":{"name":"Archiv fur Geschwulstforschung","volume":"60 2","pages":"89-96"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13313807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tumor markers--expectations and possibilities.","authors":"B von Broen","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":8274,"journal":{"name":"Archiv fur Geschwulstforschung","volume":"60 4","pages":"305-8"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13354692","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stimulation of the immune response in outpatients with hepatocellular carcinomas by low doses of cyclophosphamide (LDCY), echinacea purpurea extracts (Echinacin) and thymostimulin.","authors":"C Lersch,&nbsp;M Zeuner,&nbsp;A Bauer,&nbsp;K Siebenrock,&nbsp;R Hart,&nbsp;F Wagner,&nbsp;U Fink,&nbsp;H Dancygier,&nbsp;M Classen","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Outpatients with inoperable far advanced hepato-cellular carcinomas (n = 5) were treated with LDCY--300 mg/m2 i.v. every 28 days-, echinacin--60 mg/m2 i.m.--and thymostimulin--30 mg/m2 i.m., day 3-10 after LDCY, then twice a week. Therapy was well tolerated by all patients. Their Karnofsky' index increased for 10% in the mean. A stable disease for more than 8 weeks was documented by abdominal ultrasonography in one patient. Serum levels of Alpha-Fetoprotein (AFP), Carcinoembryonic Antigen (CEA) and Tissue Polypeptide Antigen (TPA) did not increase in 2 patients. Median survival time was 2.5 months. One patient is still alive after 8 months. Absolute numbers of CD8+ cells significantly (p less than 0.02) decreased for 7% 1 day after LDCY, whereas CD4+ cells increased (p less than 0.02) from day 1-7. Numbers of natural killer (NK-) cells increased for 17% (p less than 0.05), their activity for 90% (p less than 0.05). Activities of peripheral polymorphs (p less than 0.05) increased for 27% and of Lymphokine Activated Killer (LAK-) cells for 180% (p less than 0.05).</p>","PeriodicalId":8274,"journal":{"name":"Archiv fur Geschwulstforschung","volume":"60 5","pages":"379-83"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13327073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Intensification of hyperglycemic tumor hyperacidity by hypothermia].","authors":"M von Ardenne,&nbsp;P G Reitnauer,&nbsp;C Hentschel","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>On rats hypothermia of 22 to 20 degrees C and rewarming to normal body temperature leads to an amplification of the hyperglycemic tumor acidification. Mechanism and significance of this effect are discussed.</p>","PeriodicalId":8274,"journal":{"name":"Archiv fur Geschwulstforschung","volume":"60 5","pages":"349-55"},"PeriodicalIF":0.0,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13392811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}