Applied Sciences最新文献_第2页

Real-Time Progressive Cutting of Deformable Objects in Unity 3D with Internal Shape-Preserving Constraints 具有内部形状保持约束的Unity 3D中可变形物体的实时渐进切割

Applied Sciences Pub Date : 2025-12-09 DOI: 10.3390/app152412984

Lyudmila Dmitrievna Khan, Taeheon Kim, Min Hong

引用次数: 0

A Traveling Multi-Analyte Chemosensor Based on Wet-Chemical Colorimetry for Shipboard Seawater Analysis 一种基于湿化学比色法的船用多分析物化学传感器

Applied Sciences Pub Date : 2025-12-05 DOI: 10.3390/app152412861

Jianzhang Wang, Yingxia Wu, Jian Zhang, Shengli Wang, Hongliang Wang

{"title":"A Traveling Multi-Analyte Chemosensor Based on Wet-Chemical Colorimetry for Shipboard Seawater Analysis","authors":"Jianzhang Wang, Yingxia Wu, Jian Zhang, Shengli Wang, Hongliang Wang","doi":"10.3390/app152412861","DOIUrl":"https://doi.org/10.3390/app152412861","url":null,"abstract":"Continuous monitoring of seawater nutrients is crucial for marine resource research and conservation, yet it faces challenges due to the constraints of offshore working conditions. We developed a multi-analyte sensor based on flow analysis technology, which integrates wet-chemical colorimetry/fluorometry for the simultaneous in situ determination of nitrite, nitrate, ammonium, silicate, and phosphate in seawater. To mitigate bubble interference, an integrated gas-trapping cavity was designed, and a data-cleaning algorithm based on the interquartile range method was implemented. In June 2025, a sea trial was conducted at two stations in the northern South China Sea, the results of which showed high consistency with laboratory standard methods: the maximum absolute relative errors were 1.79% for nitrite, 5.01% for nitrate, 1.42% for ammonium, 5.93% for phosphate, and 2.95% for silicate. The performance under real marine conditions is demonstrated by relative errors below 6% and linear correlation coefficients exceeding 0.999 for all parameters. This research demonstrates a practical approach for in situ marine observation.","PeriodicalId":8224,"journal":{"name":"Applied Sciences","volume":"15 24","pages":"12861-12861"},"PeriodicalIF":0.0,"publicationDate":"2025-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2076-3417/15/24/12861/pdf?version=1764930285","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147900312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of Post-Mining Utilization Potential Using GIS and AHP: A Comparative Study of the United States and South Korea 基于GIS和AHP的采矿后利用潜力评价：美国与韩国的比较研究

Applied Sciences Pub Date : 2025-11-28 DOI: 10.3390/app152312652

Donju Lee, Hojin Kim, Sung-Min Kim

{"title":"Assessment of Post-Mining Utilization Potential Using GIS and AHP: A Comparative Study of the United States and South Korea","authors":"Donju Lee, Hojin Kim, Sung-Min Kim","doi":"10.3390/app152312652","DOIUrl":"https://doi.org/10.3390/app152312652","url":null,"abstract":"This study presents an integrated framework that combines Geographic Information System (GIS)-based spatial analysis with the Analytic Hierarchy Process (AHP) to quantitatively evaluate the tourism and educational reuse potential of abandoned mines. Five spatial indicators—population density, slope, urban ratio, road accessibility, and proximity to tourist attractions—were normalized using logarithmic transformation and cumulative distribution functions and weighted through expert-based AHP evaluation. The framework was applied to large-scale mine datasets in the United States and South Korea, followed by in-depth case validation in the Gangwon region. The results indicate that successfully redeveloped mines consistently exhibited higher composite scores than the national averages in both countries, confirming the framework’s explanatory power. The Gangwon analysis further demonstrated that mine reuse potential extends beyond tourism to include smart mining education and environmental testbed applications. Overall, this study provides a transferable GIS–AHP model for assessing post-mining utilization and supports evidence-based, region-specific policy design for sustainable mine redevelopment.","PeriodicalId":8224,"journal":{"name":"Applied Sciences","volume":"15 23","pages":"12652-12652"},"PeriodicalIF":0.0,"publicationDate":"2025-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2076-3417/15/23/12652/pdf?version=1764580221","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147909045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enzymatic Hydrolysis of Polysaccharide from Houttuynia cordata and Structure Characterization of the Degradation Products and Their α-Glucosidase Inhibitory Activity 鱼腥草多糖的酶解及其α-葡萄糖苷酶抑制活性研究

Applied Sciences Pub Date : 2025-10-15 DOI: 10.3390/app152011057

Lanlan Zhang, Zhixuan Yang, Yanan Yuan, Mohammed Mansour, Ting Wu, Siyi Pan, Xiaoyun Xu

{"title":"Enzymatic Hydrolysis of Polysaccharide from Houttuynia cordata and Structure Characterization of the Degradation Products and Their α-Glucosidase Inhibitory Activity","authors":"Lanlan Zhang, Zhixuan Yang, Yanan Yuan, Mohammed Mansour, Ting Wu, Siyi Pan, Xiaoyun Xu","doi":"10.3390/app152011057","DOIUrl":"https://doi.org/10.3390/app152011057","url":null,"abstract":"This study aimed to enhance the α-glucosidase inhibitory activity of Houttuynia cordata polysaccharide (HCP) and investigate the structure of derivatives. Under optimal conditions (amylase derived from Aspergillus oryzae loading of 15 U/mL, 60 °C, and pH 6.1), the enzymatic hydrolysates of HCP (EHCP) demonstrated significantly higher α-glucosidase inhibition than non-enzymatically treated HCP (NEHCP). At a 6 mg/mL concentration, the α-glucosidase inhibition rates of EHCP and NEHCP were 77.32% and 52.92%, respectively. Molecular weight analysis revealed that EHCP was a homogeneous polysaccharide of 338.7 kDa, lower than that of NEHCP (504.6 kDa). The monosaccharide composition was Galacturonic acid/Glucuronic acid/Galactose/Rhamnose/Mannose/Fucose/Xylose/Arabinose/Glucose = 77.42:3.78:8.04:2.12:3.16:2.48:0.75:0.17:2.08 molar ratio. Infrared and nuclear magnetic resonance analyses confirmed pyranose rings and both α- and β-glycosidic linkages. Compared with NEHCP, EHCP demonstrated improved solubility, decreased crystallinity, and morphological changes from dense rod-like to loose flaky structures.","PeriodicalId":8224,"journal":{"name":"Applied Sciences","volume":"15 20","pages":"11057-11057"},"PeriodicalIF":0.0,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2076-3417/15/20/11057/pdf?version=1760530766","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147879530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Development and Validation of an HPLC-PDA Method for NMN Quantification in Commercial Pet Foods 商用宠物食品中NMN定量的HPLC-PDA方法的建立与验证

Applied Sciences Pub Date : 2025-10-08 DOI: 10.3390/app151910797

Y. Gloria Meng, Cunhao Li, Tao Lan, Lihong Wang, Jingxuan Zhang

引用次数: 1

Enzymatic Analysis of Chitin Deacetylases on Crystalline Chitin with Varied Molecular Weights: Insights from Active Pocket Characteristic Analysis 不同分子量结晶几丁质脱乙酰酶的酶学分析：来自活性口袋特征分析的见解

Applied Sciences Pub Date : 2025-10-05 DOI: 10.3390/app151910721

Kaige Chen, Shengyu Yang, Jun Cai

{"title":"Enzymatic Analysis of Chitin Deacetylases on Crystalline Chitin with Varied Molecular Weights: Insights from Active Pocket Characteristic Analysis","authors":"Kaige Chen, Shengyu Yang, Jun Cai","doi":"10.3390/app151910721","DOIUrl":"https://doi.org/10.3390/app151910721","url":null,"abstract":"Chitin deacetylases (CDAs), which catalyze the deacetylation of chitin to produce chitosan, have garnered significant interest due to their environmental compatibility and ability to control product quality. However, the low conversion efficiency resulting from chitin’s high molecular weight and crystallinity, as well as structural limitations of CDAs, has impeded their industrial application. In this study, we present the integrated approach combining bioinformatics and computational tools (adaptive Poisson–Boltzmann solver, Fpocket, and ProteinPlus) to systematically analyze sequence features and variations in active pocket properties among CDAs from diverse origins. Experimental evaluation of the deacetylation activity of AnCDA, AsCDA, BaCDA, and ScCDA, each with distinct pocket characteristics, on chitin substrates with varying molecular parameters revealed that CDAs with high hydrophobicity scores and low surface-to-volume ratios exhibited superior efficiency in converting high-molecular-weight chitin. These findings guide the rational selection and engineering of CDAs for industrial biocatalysis.","PeriodicalId":8224,"journal":{"name":"Applied Sciences","volume":"15 19","pages":"10721-10721"},"PeriodicalIF":0.0,"publicationDate":"2025-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2076-3417/15/19/10721/pdf?version=1759647717","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147333510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Fluoride Adsorptive Removal by Metallic Phosphates 金属磷酸盐吸附除氟效果评价

Applied Sciences Pub Date : 2025-09-26 DOI: 10.3390/app151910454

Ruijie Wang, Yingpeng Gu, Mengfei Ma, Yue Sun

{"title":"Evaluation of Fluoride Adsorptive Removal by Metallic Phosphates","authors":"Ruijie Wang, Yingpeng Gu, Mengfei Ma, Yue Sun","doi":"10.3390/app151910454","DOIUrl":"https://doi.org/10.3390/app151910454","url":null,"abstract":"Currently, various techniques are efficient in eliminating high quantities of fluoride from water, while the deep treatment of a low concentration of fluoridated water is inadequate. In this work, four metallic phosphates were synthesized, including YP, ZrP, CeP, and LaP, to enhance the elimination of fluoride. The X-ray diffractometer data demonstrated that ZrP was amorphous, while CeP, LaP, and YP were highly crystalline. YP had a strong fluoride removal ability in a neutral environment, and ZrP exhibited a superior fluoride adsorption effect in acidic media. The adsorption kinetic results suggested that YP, CeP, and LaP could achieve the adsorption equilibrium within 150 min, which was faster than ZrP. YP had the largest fluoride adsorption capacity fitted by Langmuir of 31.61 mg/g at 298 K, followed by ZrP, which was greater than those of CeP and LaP. All four metallic phosphates showed high selectivity in the interference of competing anions and organics, with YP and ZrP exhibiting superior selectivity than CeP and LaP. The adsorption mechanism was ligand exchange between metallic phosphate particles and fluoride, which was validated by Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. The adsorption rate of metallic phosphates remained essentially stable in five consecutive adsorption–desorption cycles. Overall, metallic phosphates, especially YP and ZrP, have enormous potential in enhancing fluoride removal in the treatment of fluoridated water.","PeriodicalId":8224,"journal":{"name":"Applied Sciences","volume":"15 19","pages":"10454-10454"},"PeriodicalIF":0.0,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2076-3417/15/19/10454/pdf?version=1758887555","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147882150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Development and Performance Evaluation of Translucent Concrete Incorporating Activated Copper Tailings as Cementitious Material 活化铜尾矿胶凝半透明混凝土的研制及性能评价

Applied Sciences Pub Date : 2025-09-19 DOI: 10.3390/app151810228

Guangdong An, Siyang Li, Zhaorui Li, Zhaohui He, Kai Li, Ping Ning, Xiangyu Wang, Xin Sun

{"title":"Development and Performance Evaluation of Translucent Concrete Incorporating Activated Copper Tailings as Cementitious Material","authors":"Guangdong An, Siyang Li, Zhaorui Li, Zhaohui He, Kai Li, Ping Ning, Xiangyu Wang, Xin Sun","doi":"10.3390/app151810228","DOIUrl":"https://doi.org/10.3390/app151810228","url":null,"abstract":"This study reports a method for producing translucent concrete using alkali-activated copper tailings, aiming to advance the valorization of solid waste and the development of sustainable construction materials. Under optimal conditions—600 °C calcination, 10 wt% CaO, and a 1:2 water-to-solid ratio—the material achieved a maximum 28-day compressive strength of 52.7 MPa, accompanied by a significantly reduced setting time. Leaching tests indicated that Cu, Zn, Pb, and As concentrations were well below the standard limits, ensuring environmental safety. Further optimization revealed that incorporating 40 wt% cement and 2 wt% polypropylene fibers (1 mm in diameter) provided the best balance between light transmission and mechanical performance. Microstructural analyses (XRD and SEM) confirmed the formation of C–S–H and C–A–S–H gels with minor Ca(OH)2, which densified the matrix and enhanced strength. Despite these promising results, potential variations in the tailing composition and challenges associated with industrial-scale implementation must be considered. Overall, this work elucidates the hydration and solidification mechanisms of copper-tailing-based translucent concrete and highlights its potential for environmentally sustainable and functional construction materials.","PeriodicalId":8224,"journal":{"name":"Applied Sciences","volume":"15 18","pages":"10228-10228"},"PeriodicalIF":0.0,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2076-3417/15/18/10228/pdf?version=1758287937","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147886080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantitative Proteomics Based on Data-Independent Acquisition Reveals Differential Protein Expression in Sika Deer Antler-Derived Traditional Chinese Medicine Across Different Growth Periods 基于数据独立获取的定量蛋白质组学揭示梅花鹿鹿角中药在不同生长期的差异蛋白表达

Applied Sciences Pub Date : 2025-09-04 DOI: 10.3390/app15179737

Yihao Jiang, Lei Liang, Hu Zheng, Zhangfeng Ding, Zhibiao Yu

{"title":"Quantitative Proteomics Based on Data-Independent Acquisition Reveals Differential Protein Expression in Sika Deer Antler-Derived Traditional Chinese Medicine Across Different Growth Periods","authors":"Yihao Jiang, Lei Liang, Hu Zheng, Zhangfeng Ding, Zhibiao Yu","doi":"10.3390/app15179737","DOIUrl":"https://doi.org/10.3390/app15179737","url":null,"abstract":"Deer antler-derived medicinal materials, including antler velvet, antlers, and deer antler base, exhibit differential therapeutic efficacy across developmental stages, though their molecular mechanisms at the proteomic level remain uncharacterized. This study employed Data-Independent Acquisition (DIA) quantitative proteomics to systematically analyze protein profiles in sika deer antler velvet, antlers, and deer antler base. Comparative analysis revealed 3154 differentially expressed proteins (DEPs, 95% upregulated) between antler velvet and antlers, which were significantly enriched in Ribosome Biogenesis (e.g., Polyadenylate-binding protein), oxidative phosphorylation, and neurodegenerative disease pathways. In the comparison of deer antler base versus antlers, 1024 DEPs (92% upregulated) were identified, primarily involved in proteolysis (e.g., ACTC protein), glycolysis, and complement and coagulation cascades. Between deer antler base and antler velvet, 2749 DEPs (87% downregulated) were enriched in Thioredoxin domains, cytoskeleton regulation, and RNA-binding functions. Subcellular localization demonstrated antler velvet proteins predominantly distributed in the cytoplasm (37.6%) and nucleus (19.6%), while deer antler base proteins showed marked enrichment in extracellular regions (19.7%) and cytoskeletal components. As the first comprehensive proteomic characterization of these materials, this study identifies ribosomal proteins and complement pathway-related proteins as key biomarkers, thus establishing a scientific foundation for precise authentication, quality control, and efficacy–mechanism interpretation of deer antler-derived medicines. It further highlights antler velvet’s neuroprotective potential and deer antler base’s immunomodulatory applications.","PeriodicalId":8224,"journal":{"name":"Applied Sciences","volume":"15 17","pages":"9737-9737"},"PeriodicalIF":0.0,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2076-3417/15/17/9737/pdf?version=1756992310","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147893383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Three-Dimensional-Printed Lateral Extraction Enhanced Desorption Electrospray Ionization Source for Mass Spectrometry 三维印刷横向萃取增强解吸电喷雾电离源质谱分析

Applied Sciences Pub Date : 2025-08-28 DOI: 10.3390/app15179468

Jilin Liu, Xiang Qian

{"title":"Three-Dimensional-Printed Lateral Extraction Enhanced Desorption Electrospray Ionization Source for Mass Spectrometry","authors":"Jilin Liu, Xiang Qian","doi":"10.3390/app15179468","DOIUrl":"https://doi.org/10.3390/app15179468","url":null,"abstract":"This paper introduces a novel Lateral Extraction Enhanced Desorption Electrospray Ionization (LEE-DESI) source. This source is specifically designed to tackle the crucial issue of electric field interference in dual-channel ambient ionization mass spectrometry (AIMS). By incorporating dual-channel spraying-based desorption and extraction into a 3D-printed chamber with optimized spatial parameters, the system effectively reduces cross-channel interference while boosting ionization efficiency. The desorption spray is responsible for desorbing analytes from untreated samples, and the extraction spray further ionizes more neutral droplets through charge transfer, which substantially enhances sensitivity. Compared with traditional DESI, the LEE-DESI source demonstrates improved detection limits, reproducibility, and operational simplicity, as validated using Rhodamine B, L-arginine, and Angiotensin I, as well as drug standards including methadone, ketamine, and fentanyl. This highlights its potential for high-throughput analysis of complex matrices in proteomics, metabolomics, and biomedical applications.","PeriodicalId":8224,"journal":{"name":"Applied Sciences","volume":"15 17","pages":"9468-9468"},"PeriodicalIF":0.0,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.mdpi.com/2076-3417/15/17/9468/pdf?version=1756395023","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147901569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1