Archives of biochemistry and biophysics最新文献

{"title":"Corrigendum to: “New CYP154C4 from Streptomyces cavourensis YBQ59 performs regio- and stereo-selective 3β-hydroxylation of nootkatone” [Arch. Biochem. Biophys. 762 (2024) 110192]","authors":"Thuy T.B. Ly , Thu-Thuy Thi Mai , Alessandra Raffaele , Vlada B. Urlacher , Thi Thao Nguyen , Michael C. Hutter , Hanh-Nguyen Thi Vu , Duong Thi Thuy Le , Tung Ngoc Quach , Quyet-Tien Phi","doi":"10.1016/j.abb.2025.110527","DOIUrl":"10.1016/j.abb.2025.110527","url":null,"abstract":"","PeriodicalId":8174,"journal":{"name":"Archives of biochemistry and biophysics","volume":"771 ","pages":"Article 110527"},"PeriodicalIF":3.8,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Causal role of phenylalanine and cholesterol metabolism in bladder cancer Chemoresistance: Insights from mendelian randomization and targeted molecular docking","authors":"Junyu Li ,&nbsp;Qianya Chen ,&nbsp;Jing wang ,&nbsp;Zhichao Wang ,&nbsp;Liumeng Jian ,&nbsp;Guangda Yang","doi":"10.1016/j.abb.2025.110530","DOIUrl":"10.1016/j.abb.2025.110530","url":null,"abstract":"<div><div>Bladder cancer (BLCA) exhibits profound chemoresistance, partly mediated by metabolic dysregulation. This study investigated the roles of phenylalanine (Phe) and cholesterol metabolism in BLCA chemoresistance using integrated omics and computational modeling. Transcriptomic analysis of the Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) datasets revealed that elevated hexokinase 2 (HK2) and low-density lipoprotein receptor (LDLR) expression correlates with poor response to cisplatin. Mechanistically, Phe catabolism via phenylalanine hydroxylase (PAH) activated mTORC1 signaling, promoting glycolysis and drug efflux, while cholesterol biosynthesis driven by SREBP2 enhanced ABCG2 transporter activity. Mendelian randomization (MR) analyses confirmed causal associations between Phe/cholesterol metabolism and chemoresistance risk. Pharmacological inhibition of HMG-CoA reductase or dietary phenylalanine restriction sensitized BLCA cells to cisplatin both <em>in vitro</em> and in xenograft models. Overexpression of FOLH1 or F7 reversed the inhibitory effects of Phe or cholesterol targeting, respectively, confirming their functional roles in resistance. These findings identify FOLH1 and F7 as mediators of metabolic resistance in BLCA and suggest metabolic targeting as a promising therapeutic strategy. However, whether combined inhibition of Phe and cholesterol pathways provides synergistic benefits over monotherapy remains to be determined in future studies.</div></div>","PeriodicalId":8174,"journal":{"name":"Archives of biochemistry and biophysics","volume":"772 ","pages":"Article 110530"},"PeriodicalIF":3.8,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preserving antimicrobial efficacy while extending peptide longevity: effects of residue glycosylation","authors":"Ilaria Di Donato ,&nbsp;Attila Tortorella ,&nbsp;Marco Campanile ,&nbsp;Andreas Rumpel ,&nbsp;Linda Leone ,&nbsp;Andrea Bosso ,&nbsp;Luigi Petraccone ,&nbsp;Angelina Lombardi ,&nbsp;Roland Winter ,&nbsp;Pompea Del Vecchio ,&nbsp;Rosario Oliva","doi":"10.1016/j.abb.2025.110526","DOIUrl":"10.1016/j.abb.2025.110526","url":null,"abstract":"<div><div>The spread of antibiotic-resistant bacteria has prompted the search for new drugs. Antimicrobial peptides (AMPs) are promising candidates, but their pharmacological application is limited by their poor stability, especially against proteolytic enzymes. A strategy to increase AMPs half-life is the introduction of sugars at key residues, a process termed glycosylation. In this work, the RLK10 peptide was obtained from the GKY10 peptide, by replacing the glutamine residue with an asparagine. Then, it was glycosylated at the asparagine by introducing a N-acetylglucosamine, obtaining the peptide RLK10-NAG. To further increase the stability, the N-terminus was acetylated, producing the Ac-RLK10-NAG peptide<em>. In cellulo</em> assays revealed that RLK10 modifications limitedly affect its antimicrobial activity. However, using POPE/POPG liposomes as a bacterial model membrane, marked differences in their mode of action were found. Stability tests against proteases revealed that the N-acetylglucosamine significantly enhances the stability of the RLK10 sequence. Unexpectedly, the acetylation didn't improve the peptide resistance against proteases, rather it increased its degradation susceptibility. These results demonstrate that glycosylation is an effective strategy to improve the AMPs stability while marginally affecting their biological activity, opening to the possibility of using AMPs in medicine and extending their use into other areas such as food preservation.</div></div>","PeriodicalId":8174,"journal":{"name":"Archives of biochemistry and biophysics","volume":"771 ","pages":"Article 110526"},"PeriodicalIF":3.8,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144548667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ameliorative effect of palonosetron against binge alcohol-induced neurodamage via targeting miR-155/AKT/mTOR/AMPK-mediated autophagic pathway","authors":"Muhammad A.E. Saad ,&nbsp;Rabab Hamed Sayed ,&nbsp;Ayman E. El-Sahar ,&nbsp;Noha H. Sayed ,&nbsp;Mona A. Kortam ,&nbsp;Nevine Fathy","doi":"10.1016/j.abb.2025.110525","DOIUrl":"10.1016/j.abb.2025.110525","url":null,"abstract":"<div><div>Binge drinking (BD) is heavy episodic alcohol drinking that is progressively practiced. Vast evidence verified that BD elicits neuronal and cognitive impairments. Debilitated autophagic machinery is a key culprit in BD-induced neurotoxicity. Palonosetron is a potent selective serotonin 5-HT3 receptor antagonist whose impact on BD has not yet been scrutinized. Thus, the present study aimed at exposing the potentiality of palonosetron and its link with AKT/mTOR/AMPK/ULK1 pathway in the BD-rat model. Rats were divided into 4 groups; group 1 received saline and Vanilla Ensure® Plus, whereas groups 2, 3 and 4 received 20 % w/v ethanol in Vanilla Ensure® Plus (intragastric gavage) every 8 h for 4 days, concomitantly with palonosetron (0.1 mg/kg, twice daily; i.p.) in groups 3 and 4, and chloroquine (50 mg/kg/day; i.p.) in group 4. BD impaired memory, locomotor, and cognitive functions, with concomitant TNF-α and IL-1β elevation implicating neuroinflammation-driven cognitive decline. The former effect was, mechanistically, triggered by halting autophagy via augmenting hippocampal pAKT/tAKT, pmTOR/tmTOR and pULK1/tULK1 ratios, while reducing pAMPK/tAMPK, with resultant imbalance of the autophagic markers; Beclin-1, LC3-II/LC3-I, p62 and caspase-3. Aberrant upregulation of miRNA-155 was also detected and was markedly correlated to AKT/mTOR/AMPK and autophagy trajectories. Palonosetron treatment significantly alleviated all the aforementioned deviations. Histopathological analysis further corroborated palonosetron neuroprotective effect. Chloroquine, a classical autophagy inhibitor, blunted palonosetron-induced improvement. The identified parameters were validated using the ShinyGO-0.81 database for functional enrichment analysis and KEGG pathway mapping. For the first time, palonosetron is likely to offer a reliable neuroprotective effect in BD via orchestrating the crosstalk between miRNA-155 and AKT/mTOR/AMPK signaling cascade.</div></div>","PeriodicalId":8174,"journal":{"name":"Archives of biochemistry and biophysics","volume":"771 ","pages":"Article 110525"},"PeriodicalIF":3.8,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144523671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Direct iron-protein coordination is dispensable for heme trafficking and the tetrapyrrole reductase activity of HmuF from Fusobacterium nucleatum","authors":"Alexandra K. McGregor,&nbsp;Long T.M. Do,&nbsp;Alexis Gauvin,&nbsp;Kirsten R. Wolthers","doi":"10.1016/j.abb.2025.110520","DOIUrl":"10.1016/j.abb.2025.110520","url":null,"abstract":"<div><div>HmuF from <em>Fusobacterium nucleatum</em> is a new member of the flavodoxin superfamily that traffics heme to anaerobilin synthase for decyclization of the porphyrin ring. HmuF then catalyzes the reduction of the linearized tetrapyrrole, termed anaerobilin. Sequence alignment of HmuF homologs of other gram-negative anaerobes reveals that the iron-coordinating histidine residue (H134) is not conserved in HmuF homologs of <em>Leptotrichia</em> and <em>Campylobacter.</em> Given this lack of sequence conservation, we created H134F, H134Y, H134A, H134C and H134M variants of HmuF to explore the importance of H134 on HmuF function. All variants retained the FMN cofactor and were purified with sub-stoichiometric amounts of heme bound to the protein. The electronic absorption spectra of the Fe³⁺-heme complexes suggest that the ferric heme is high-spin, and among the variants, only H134Y directly coordinates with the iron. Difference spectroscopy showed that each variant binds one equivalent of heme, and competition experiments with apomyoglobin reveal the heme dissociation rate constants (<em>k</em>_off) for HmuF H134Y and H134C are comparable to wild-type HmuF, while HmuF H134A, HmuF H134M, and HmuF H134F exhibit 8-, 15- and 200-fold faster <em>k</em>_off values, respectively. All variants can traffic heme to anaerobilin synthase for radical-mediated decyclization of the heme and catalyze the subsequent reduction of the linearized tetrapyrrole. Combined, these studies demonstrate that van der Waals interactions between the heme macrocycle and the protein, as well as the FMN cofactor, regulate heme binding affinity. Direct protein-iron coordination is also not essential for heme trafficking and anaerobilin reductase activities of HmuF.</div></div>","PeriodicalId":8174,"journal":{"name":"Archives of biochemistry and biophysics","volume":"771 ","pages":"Article 110520"},"PeriodicalIF":3.8,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144501350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding the mechanism involved in lung epithelial potassium current modulation by ozone","authors":"Rita Canella ,&nbsp;Mascia Benedusi ,&nbsp;Giulia Trinchera ,&nbsp;Angela Pignatelli ,&nbsp;Giuseppe Valacchi","doi":"10.1016/j.abb.2025.110522","DOIUrl":"10.1016/j.abb.2025.110522","url":null,"abstract":"<div><div>Given that the ionic balance of the pulmonary alveolar lining fluid is essential for correct gas exchange, our previous studies analyzed the effects of ozone (O₃), one of the most harmful pollutants for the respiratory system, on K⁺ current (I_K)in human cultured lung epithelial cells (A549). O₃ exposure significantly alters the flow of K⁺ ions, reducing the outward rectifier current component. O₃ does not act directly, but through its byproducts, among which the main ones are 4-hydroxy-2-nonenal (4HNE) and hydrogen peroxide (H₂O₂).</div><div>In this study we analyzed the action of 4HNE and H₂O₂ on I_K in human lung cells and verified the protective effect of catalase.</div></div><div><h3>Material</h3><div>A549 cellular line. 30’ exposure: O₃: 0.1 ppm; 4HNE: 5, 10 and 20 μM; H₂O₂: 20 μM. Glucose-oxidase: 10 mU/l, 1.00h exposure, analyzed after 24h. Catalase: 1000 U, 1.30h exposure.</div></div><div><h3>Methods</h3><div>Western blot, immunofluorescence and patch clamp techniques to study the action of the bio-products on I_K.</div><div>4HNE was able to significantly decrease the I_K, but it was less effective than O₃. H₂O₂ produced by the cells after GO administration completely reproduced the O₃ effect. Catalase showed its ability in preserving the outward rectifier component depressed by O₃, bringing back the current to the control level.</div><div>We can conclude that 4HNE and H₂O₂ are responsible for the O₃ action on potassium channels. The protective role of catalase confirms the ability of O₃ bio-product to modify the cellular redox homeostasis. In perspective it will be interesting to analyze their interaction, and the bio-molecular pathways activated.</div></div>","PeriodicalId":8174,"journal":{"name":"Archives of biochemistry and biophysics","volume":"771 ","pages":"Article 110522"},"PeriodicalIF":3.8,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144526229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Instability and resilience at the lipid membrane interface under ultrasound: composition matters","authors":"Alexandre Blanco-González ,&nbsp;Ángel Piñeiro ,&nbsp;Rebeca García-Fandiño","doi":"10.1016/j.abb.2025.110523","DOIUrl":"10.1016/j.abb.2025.110523","url":null,"abstract":"<div><div>Lipid membranes play a crucial role in cellular function, acting not only as structural barriers but also facilitating key biological processes such as selective permeability, signaling, and mechanical stability. The composition of these membranes varies significantly across different cell types, species, and disease states, influencing their mechanical properties and susceptibility to disruption. This variability presents an opportunity to selectively target pathological cells based on their unique lipid profiles, potentially allowing for the precise disruption of diseased cells while sparing healthy ones. Additionally, focused ultrasound (FUS) has emerged as a promising tool for modulating membrane integrity, with applications in targeted drug delivery and cancer therapy. However, the precise interactions between FUS waves and different lipid compositions remain insufficiently understood. This study systematically investigates the effects of varying ultrasound frequencies (5–50 MHz) and overpressures (5–50 bar) on the mechanical responses of four distinct lipid bilayers—POPC, POPE, POPG, and POPS—using molecular dynamics simulations. These lipids are commonly found in mammalian, bacterial, and cancerous cell membranes. Key structural parameters, including area per lipid, curvature, thickness, and lipid tail order, were analyzed to determine how different ultrasound conditions affect membrane integrity. The results reveal that lipid composition critically determines membrane vulnerability to mechanical perturbations. For instance, POPC membranes are more prone to deformation under certain ultrasound conditions, while POPG and POPS exhibit abrupt transitions to instability at extreme pressures and frequencies. These findings offer valuable insights into the selective tuning of ultrasound parameters for therapeutic applications and highlight the critical role of membrane composition in determining mechanical responses to ultrasound-induced stress.</div></div>","PeriodicalId":8174,"journal":{"name":"Archives of biochemistry and biophysics","volume":"771 ","pages":"Article 110523"},"PeriodicalIF":3.8,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144526227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of active site residues involved in substrate binding and cofactor specificity in a putrescine N-monooxygenase","authors":"Noah S. Lyons ,&nbsp;Robert A. Zalenski II ,&nbsp;Pablo Sobrado","doi":"10.1016/j.abb.2025.110519","DOIUrl":"10.1016/j.abb.2025.110519","url":null,"abstract":"<div><div>The putrescine <em>N</em>-monooxygenase (NMO) FbsI from <em>Acinetobacter baumannii</em> is a flavin-dependent enzyme that catalyzes the NADPH-dependent hydroxylation of putrescine to <em>N</em>-hydroxyputrescine, an important component of the siderophore fimsbactin A. Here, we probe the roles of T240, D390, and K223 in substrate binding and cofactor recognition. Site-directed mutagenesis and biochemical characterization showed that mutation of T240 to alanine resulted in a &gt;500-fold increase in the <em>K</em>_M for putrescine, with little effect on the <em>k</em>_cat value, highlighting the importance of this residue in binding. Mutation of D390 to alanine and asparagine rendered insoluble or inactive protein, respectively, suggesting this residue is essential for catalysis. Specificity for NAD(P)H was probed by mutating K223 to alanine and arginine. The K223R mutant had a 9-fold lower <em>K</em>_M with NADPH, while K223A had a 2-fold lower <em>k</em>_cat value and minimal change to the <em>K</em>_M value when compared to wild-type (WT) enzyme. However, rapid-reaction kinetics showed that K223R had a &gt;15-fold lower <em>K</em>_D with NADPH while K223A had a 3-fold higher <em>K</em>_D and 7.5-fold lower <em>k</em>_red compared to WT. These results demonstrate that mutation of K223 to arginine increases the specificity and efficiency of the enzyme for NADPH, identifying a key residue in cofactor recognition in FbsI.</div></div>","PeriodicalId":8174,"journal":{"name":"Archives of biochemistry and biophysics","volume":"771 ","pages":"Article 110519"},"PeriodicalIF":3.8,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144517873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antidiabetic properties of dihydrooxazole Derivatives: In vitro and in silico evaluation as potential aldose reductase and α-glucosidase inhibitors","authors":"Busra Ozturk Aydin ,&nbsp;Baris Anil ,&nbsp;Yeliz Demir ,&nbsp;Aleksandra Rakić ,&nbsp;Dušan Dimić ,&nbsp;Derya Aktas Anil","doi":"10.1016/j.abb.2025.110521","DOIUrl":"10.1016/j.abb.2025.110521","url":null,"abstract":"<div><div>Proteins included in type 2 diabetes mellitus are potential targets for minimizing the disease progression. In this contribution, fourteen cinnamoyl compounds were synthesized and characterized, leading to five new oxazole derivatives. Their structures were optimized at the B3LYP/6–311++G(d,p) level of theory, and the global and local reactivity parameters were calculated. Based on these parameters, the reactive sites were determined. The experimental inhibitory effect towards aldose reductase (ALR2) and α-glucosidase (α-Glu) was followed, with some of the compounds showing higher activity than standard compounds, epalrestat, and acarbose. The interactions at the molecular level were investigated by molecular docking simulation, and the specific binding explained the relative reactivity order. The toxicity of compounds was assessed through ecotoxicology examination.</div></div>","PeriodicalId":8174,"journal":{"name":"Archives of biochemistry and biophysics","volume":"771 ","pages":"Article 110521"},"PeriodicalIF":3.8,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144526226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular interactions of chiral ionic liquids with fungal membranes: thermodynamic and molecular dynamics simulation insights","authors":"Anita Wnętrzak ,&nbsp;Joanna Feder-Kubis ,&nbsp;Anna Chachaj-Brekiesz ,&nbsp;Krzysztof Łukawski ,&nbsp;Jan Kobierski ,&nbsp;Aneta D. Petelska ,&nbsp;Patrycja Dynarowicz-Latka","doi":"10.1016/j.abb.2025.110524","DOIUrl":"10.1016/j.abb.2025.110524","url":null,"abstract":"<div><div>The development of selective antifungal agents is crucial to improve therapeutic options while minimizing side effects. This study assessed the potential antimicrobial efficacy of ionic liquids, particularly against fungal pathogens. For this, a functionalized chiral ionic liquid (FCIL) with a naturally occurring (1<em>R</em>,2<em>S</em>,5<em>R</em>)-(−)-menthol moiety and a long alkyl chain was synthesized and characterized using spectral and thermal methods. The antifungal potential of this FCIL was evaluated by examining interactions with artificial fungal and mammalian membranes modeled as Langmuir monolayers. Thermodynamic analyses, complemented by adsorption and penetration experiments, Brewster angle microscopy, polarization modulation infrared reflection absorption spectroscopy, and molecular dynamics simulations, showed that FCIL incorporated into membranes and caused fungal membrane disintegration. This can be related to <em>π-π</em> interactions with ergosterol, a primary fungal membrane sterol, and favorable assimilation into membranes containing dioleoylphosphatidylcholine, an unsaturated phospholipid abundant in fungal cells. Conversely, interactions with mammalian membranes modeled using dipalmitoylphosphatidylcholine and cholesterol were thermodynamically unfavorable due to their tighter packing. These findings underline the FCIL's ability to selectively disrupt fungal membranes and suggest its potential use as a targeted antifungal agent with reduced mammalian cell toxicity. This research highlights the benefit of integrating experimental and computational methods to understand the molecular mechanics driving selective antifungal activity.</div></div>","PeriodicalId":8174,"journal":{"name":"Archives of biochemistry and biophysics","volume":"771 ","pages":"Article 110524"},"PeriodicalIF":3.8,"publicationDate":"2025-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144526228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}