Archives of Biological Sciences最新文献_第9页

Histopathology of Chironomus riparius (Diptera, Chironomidae) exposed to metal oxide nanoparticles 金属氧化物纳米颗粒对河滨手蛾(双翅目，手蛾科)组织病理学的影响

IF 0.8 4区生物学

Archives of Biological Sciences Pub Date : 2021-01-01 DOI: 10.2298/ABS210515025S

J. Stojanović, D. Milošević, Jelena S. Vitorović, Dimitrija Savić-Zdravković, N. Stanković, J. Stanković, P. Vasiljević

{"title":"Histopathology of Chironomus riparius (Diptera, Chironomidae) exposed to metal oxide nanoparticles","authors":"J. Stojanović, D. Milošević, Jelena S. Vitorović, Dimitrija Savić-Zdravković, N. Stanković, J. Stanković, P. Vasiljević","doi":"10.2298/ABS210515025S","DOIUrl":"https://doi.org/10.2298/ABS210515025S","url":null,"abstract":"As the production of metal-based nanomaterials increases, it is inevitable that nano-scale products and byproducts will enter the aquatic environment. In terms of global production, the most abundant nano-oxides are TiO2, CeO2 and Fe3O4 nanoparticles. Chironomus riparius is commonly used for ecotoxicological assessment and defining its histopathological biomarkers that showcase the toxic effect of tested nanoparticles should lead to a better understanding of the consequences of nanomaterial accumulation in aquatic ecosystems. In this study, a histological description of the digestive and excretory systems as well as the fat body structure of C. riparius larvae is provided. In addition, potential histological biomarkers of nano-oxide toxicity were determined based on the obtained histopathological alterations in organs. Vacuolization was observed in epithelial cells of midgut region I that were treated with nano-Fe3O4 as well as in Malpighian tubules treated with nano-Fe3O4 and nano-CeO2. Larvae exposed to nano-TiO2 showed alterations in the fat body and midgut region II tissue architecture. Additionally, shortening of the intestinal brush border was determined in groups exposed to nano-Fe3O4. These results reveal the high sensitivity of these organs, which can be used as biomarkers in histopathological assessment and therefore lead to further improvement of existing methodology in ecotoxicological studies.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68390043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Altered diversity of bacterial communities in two Drosophila species under laboratory conditions and lead exposure 实验室条件和铅暴露下两种果蝇细菌群落多样性的改变

IF 0.8 4区生物学

Archives of Biological Sciences Pub Date : 2021-01-01 DOI: 10.2298/abs200911054b

Mirjana Beribaka, I. Dimkić, Mihailo Jelić, S. Stanković, N. Pržulj, M. Andjelković, M. Stamenković-Radak

{"title":"Altered diversity of bacterial communities in two Drosophila species under laboratory conditions and lead exposure","authors":"Mirjana Beribaka, I. Dimkić, Mihailo Jelić, S. Stanković, N. Pržulj, M. Andjelković, M. Stamenković-Radak","doi":"10.2298/abs200911054b","DOIUrl":"https://doi.org/10.2298/abs200911054b","url":null,"abstract":"The composition of microbiota affects different traits of Drosophila throughout its life cycle and represents an important part of the adaptive response to environmental changes, such as increased levels of accumulated heavy metals in their habitats. We investigated the effects of lead added to the feeding substrate on the microbiota diversity in the fruit flies, Drosophila melanogaster and Drosophila subobscura. We compared the bacterial diversity of wild-caught flies and their progeny reared under standard laboratory conditions. We analyzed the shifts in bacterial diversity in the experimental groups of flies reared for 13 generations on standard and lead(II) acetate (Pb(CH3COO)2), Pb acetate-saturated substrates. Identification of the main bacterial genera was performed by next-generation sequencing (NGS) of the V3-V4 variable regions of the 16S rRNA gene. Results indicate significant species-specific differences in bacterial composition between natural and laboratory populations, and between the substrates. Diversity increased in both species under prolonged exposure to lead-polluted substrate, suggesting a potential adaptive response to an environmental stress.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68388347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Diurnal photoperiods and rhythmicity of the phototropic bending response in hypocotyls of sunflower, Helianthus annuus L. seedlings 向日葵(Helianthus annuus L.)幼苗下胚轴致光弯曲响应的日光周期和节律性

IF 0.8 4区生物学

Archives of Biological Sciences Pub Date : 2021-01-01 DOI: 10.2298/ABS210205018V

D. Vinterhalter, B. Vinterhalter

{"title":"Diurnal photoperiods and rhythmicity of the phototropic bending response in hypocotyls of sunflower, Helianthus annuus L. seedlings","authors":"D. Vinterhalter, B. Vinterhalter","doi":"10.2298/ABS210205018V","DOIUrl":"https://doi.org/10.2298/ABS210205018V","url":null,"abstract":"Research on phototropic (PT) bending in sunflower (Helianthus annuus L. cv. Kondi (Syngenta)) seedling hypocotyls presented herein focused on a comparison of diurnal and free-running photoperiods with the aim of explaining the development of diurnal rhythmicity. PT bending magnitudes and lag phase duration exhibited strong daily rhythmicity in all diurnal photoperiods, contrasting with the uniform PT bending response to constant light (CL) conditions. Plants had a daytime maximum for PT bending magnitudes in experiments starting around midday and a minimum in the dark period in those starting 4 h after dusk. Plants could compensate for large differences in the daytime duration of diurnal photoperiods. They required the first 4 h of darkness to recover and synchronize the PT bending and to start increasing the magnitudes of PT bending. The daily pattern of lag phase duration changes was similar but inverted, showing that synchronization also occurred during nighttime. Darkness was not required for PT bending under CL conditions, however, during diurnal photoperiods it enabled the establishment of diurnal rhythmicity and synchronized changes in PT bending capacity to occur when needed, providing maximal values at midday and minimal during the nighttime. Under prolonged duration of daytime corresponding to the start of CL condition, plantlets rapidly abandoned circadian regulation, their PT bending response becoming arrhythmic.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68388603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

SIRT2 mediated downregulation of FOXM1 in response to TGFβ through the RAF-MEK-ERK signaling pathway in colon cancer 在结肠癌中，SIRT2通过RAF-MEK-ERK信号通路介导FOXM1对TGFβ的下调

IF 0.8 4区生物学

Archives of Biological Sciences Pub Date : 2021-01-01 DOI: 10.2298/ABS210227020O

Ozkan Ozden, S. Park

{"title":"SIRT2 mediated downregulation of FOXM1 in response to TGFβ through the RAF-MEK-ERK signaling pathway in colon cancer","authors":"Ozkan Ozden, S. Park","doi":"10.2298/ABS210227020O","DOIUrl":"https://doi.org/10.2298/ABS210227020O","url":null,"abstract":"The transcription factor forkhead box M1 (FOXM1) is frequently upregulated in many solid tumors, including those in the colon. As a master regulator, the sirtuin (SIRT) protein family is comprised of seven nicotinamide adenine dinucleotide (NAD+)-dependent deacetylases/adenosine diphosphate (ADP) ribosyl transferases whose activities are associated with aging and cancer. In this study, we determined whether a cytoplasmic member of SIRTs, SIRT2, influences the expression of oncogenic FOXM1 in colon cancer in vitro. The association of SIRT2 and FOXM1 were analyzed using SIRT2 knockout mouse embryonic fibroblasts and SIRT2 knocked-down and overexpressing HCT116 colon cancer cell lines. Cell lines were treated with 10 ng/mL transforming growth factor-beta (TGFR) for 24 h. SIRT2 could downregulate FOXM1 through the TGF? mitogen-activated protein kinase (RAF-MEK-ERK) signaling pathway in genetically altered mouse embryonic fibroblasts and colon cancer cell lines. The indirect association between SIRT2 and FOXM1 through TGF? may be important because activators or inhibitors of SIRT2 could provide a potential approach to downregulate FOXM1 in gastrointestinal cancers.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68388634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The single nucleotide polymorphisms rs11761556 and rs12706832 of the leptin gene are associated with type 2 diabetes mellitus in the Iraqi population 伊拉克人群瘦素基因单核苷酸多态性rs11761556和rs12706832与2型糖尿病相关

IF 0.8 4区生物学

Archives of Biological Sciences Pub Date : 2021-01-01 DOI: 10.2298/ABS210129005M

N. Musafer, Z. F. Huyop, J. Ewadh, E. Supriyanto, M. Al-Thuwaini, M. Baqur

{"title":"The single nucleotide polymorphisms rs11761556 and rs12706832 of the leptin gene are associated with type 2 diabetes mellitus in the Iraqi population","authors":"N. Musafer, Z. F. Huyop, J. Ewadh, E. Supriyanto, M. Al-Thuwaini, M. Baqur","doi":"10.2298/ABS210129005M","DOIUrl":"https://doi.org/10.2298/ABS210129005M","url":null,"abstract":"This study was conducted to assess the potential association between leptin (LEP) gene polymorphisms and type 2 diabetes mellitus (T2DM) in Iraqi patients. Genomic DNA was extracted from 120 diabetic subjects and 100 controls. Three specific PCR fragments were designed to flank three highly frequent single nucleotide polymorphism (SNP)s within LEP, rs11761556, rs12706832 and rs2167270. The amplified loci were genotyped by PCR-single-strand conformation polymorphism (SSCP) followed by Sanger sequencing for representative genotypes. Logistic regression analysis was performed to detect the association between the targeted genetic variants and T2DM. PCR-SSCP genotyping showed three banding patterns for all three targeted SNPs. Individuals with the AA genotype in both rs11761556 and rs12706832 SNPs showed significantly higher (P<0.05) body mass index (BMI), waist circumference (WC), fasting blood glucose (FBG), hemoglobin A1c (HbA1c), homeostatic model assessment for insulin resistance (HOMA-IR), insulin, low-density lipoprotein cholesterol (LDL-C) and triglyceride (TG) values than those with other genotypes. Association analysis revealed that individuals with the A allele exhibited a greater risk of T2DM. Data of the present investigation indicated that both rs11761556 and rs12706832 SNPs exerted a noticeable association with T2DM. The study suggests implementing both rs11761556 and rs12706832 SNPs in the early detection of T2DM.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68389076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Raman spectral analysis of the brainstem and responses of neuroglia and cytokines in whole-body gamma-irradiated rats after administration of aminothiol-based radioprotector GL2011 给药氨基硫醇基放射保护剂GL2011后，全身γ辐照大鼠脑干的拉曼光谱分析和神经胶质细胞和细胞因子的反应

IF 0.8 4区生物学

Archives of Biological Sciences Pub Date : 2021-01-01 DOI: 10.2298/ABS210129011K

Dušica M Kočović, D. Bajuk-Bogdanović, I. Maslovarić, Biljana Bozic-Nedeljkovic, P. Andjus, M. Daković

{"title":"Raman spectral analysis of the brainstem and responses of neuroglia and cytokines in whole-body gamma-irradiated rats after administration of aminothiol-based radioprotector GL2011","authors":"Dušica M Kočović, D. Bajuk-Bogdanović, I. Maslovarić, Biljana Bozic-Nedeljkovic, P. Andjus, M. Daković","doi":"10.2298/ABS210129011K","DOIUrl":"https://doi.org/10.2298/ABS210129011K","url":null,"abstract":"The search for an effective and non-toxic radioprotector is ongoing. We tested a novel, natural aminothiol-based radioprotector, GL2011, that was applied 30 min, 3 h or 6 h after the exposure of male albino Wistar rats to a 6.7 Gy mild dose of gamma radiation. The molecular signatures of radioprotection were investigated with Raman microspectroscopy of brainstem tissue samples. Morphological changes and activation of astrocytes and microglia were assessed by immunohistochemistry. Global markers of neuroinflammation were followed by ELISA to monitor blood plasma levels of proinflammatory (IL-6 and TNF-?) and anti-inflammatory (IL-10) cytokines. A thirty-day follow-up determined survival of unprotected animals 37.5%. A survival increase was observed after radioprotection (75%, irrespective of the time of application). Raman spectra revealed a slightly deleterious effect of radiation on nucleic acids in surviving animals that was mitigated with the radioprotector, as GL2011 preserved the morphology of both astrocytes and microglia, with reduced microglial infiltration. Cytokine assessment revealed an immunomodulatory effect of the novel radioprotector. The overall results point out the positive effects of a single dose of GL2011 applied at different times. The molecular and cellular changes in the brainstem indicate that the radioprotector applied after radiation conferred better protection, which underlines its translation to cure radiation accidents.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68389090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

All-trans retinoic acid prevents oxidative stress-mediated cellular senescence via upregulation of insulin-like growth factor binding protein-6 in normal human epidermal keratinocytes 全反式维甲酸通过上调正常人表皮角质形成细胞中的胰岛素样生长因子结合蛋白-6来防止氧化应激介导的细胞衰老

IF 0.8 4区生物学

Archives of Biological Sciences Pub Date : 2021-01-01 DOI: 10.2298/abs210615036c

Hyangtae Choi, J. Lee, N. Park, Jin Nam, W. Park, C. Lee, B. Kim

{"title":"All-trans retinoic acid prevents oxidative stress-mediated cellular senescence via upregulation of insulin-like growth factor binding protein-6 in normal human epidermal keratinocytes","authors":"Hyangtae Choi, J. Lee, N. Park, Jin Nam, W. Park, C. Lee, B. Kim","doi":"10.2298/abs210615036c","DOIUrl":"https://doi.org/10.2298/abs210615036c","url":null,"abstract":"All-trans retinoic acid (ATRA) influences cellular proliferation and differentiation but its mechanisms of action are not understood in keratinocytes. To investigate the potential mechanisms of action of ATRA in keratinocytes, microarray analysis of ATRA-treated normal human epidermal keratinocytes (NHEKs) was performed. Based on microarray data, we focused on insulin-like growth factor binding protein-6 (IGFBP-6), which is known to inhibit cellular senescence but has not been previously investigated in the context of ATRA-induced signaling in NHEKs. We verified that ATRA significantly increased IGFBP-6 gene and protein expression in NHEKs. Next, the effects of ATRA and IGFBP-6 on cell proliferation and senescence in H2O2-treated NHEKs were examined. IGFBP-6 was knocked-down using siRNA or overexpressed using pCMV-IGFBP-6. Cellular proliferation was observed using the bromodeoxyuridine (BrdU) incorporation assay. Cellular senescence was determined by monitoring SA-?-Gal staining and p21 expression. When IGFBP-6 was knocked down, cellular proliferation was inhibited, and the cellular senescence markers were increased. IGFBP-6 overexpression or ATRA treatment of H2O2-treated NHEKs rescued these effects. Taken together, our results suggest that ATRA prevents premature senescence-related skin damage at least in part by increasing IGFBP-6 expression, as shown herein in reactive oxygen species (ROS)-stimulated NHEKs.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68389602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

rs2682818/miR-618 is a novel marker associated with increased risk of breast cancer in the Iranian population rs2682818/miR-618是伊朗人群中与乳腺癌风险增加相关的新标志物

IF 0.8 4区生物学

Archives of Biological Sciences Pub Date : 2021-01-01 DOI: 10.2298/abs210808039n

Atefeh Najafian-Najafabady, Nasim Ebrahimi, Sadeq Vallian

引用次数: 1

Antibacterial, antioxidant, cytotoxic effects and GC-MS analysis of mangrove-derived Streptomyces achromogenes TCH4 extract 红树来源消色链霉菌TCH4提取物的抗菌、抗氧化、细胞毒作用及GC-MS分析

IF 0.8 4区生物学

Archives of Biological Sciences Pub Date : 2021-01-01 DOI: 10.2298/ABS210320017T

J. Tangjitjaroenkun, W. Pluempanupat, Rungnapa Tangchitcharoenkhul, Waraporn Yahayo, R. Supabphol

{"title":"Antibacterial, antioxidant, cytotoxic effects and GC-MS analysis of mangrove-derived Streptomyces achromogenes TCH4 extract","authors":"J. Tangjitjaroenkun, W. Pluempanupat, Rungnapa Tangchitcharoenkhul, Waraporn Yahayo, R. Supabphol","doi":"10.2298/ABS210320017T","DOIUrl":"https://doi.org/10.2298/ABS210320017T","url":null,"abstract":"The in vitro biological activities of the ethyl acetate extract of the culture filtrate from Streptomyces achromogenes TCH4 (TCH4 extract) were evaluated. The ethyl acetate extract of TCH4 produced a bacteriostatic effect against Enterobacter cloacae, Staphylococcus aureus, Staphylococcus saprophyticus, Bacillus subtilis, methicillin-resistant S. aureus (MRSA) and Klebsiella pneumoniae. The extract had bactericidal activity against S. aureus, S. saprophyticus, S. aureus (MRSA) and K. pneumoniae with minimum bactericidal concentration (MBC) values in the range of 500-1000 ?g/mL. The total phenolic and flavonoid contents in TCH4 extract were 107.20?}2.57 mg gallic acid equivalent (GAE)/g and 44.91?}0.84 mg quercetin equivalent (QE)/g of dry extract. Antioxidant activity was determined by DPPH radical (IC50 299.64?}6.83 ?g/mL) and ABTS radical scavenging (IC50 65.53?}0.95 ?g/mL), and the ferric-reducing antioxidant power (FRAP) (822.76?}9.12 mM FeSO4.7H2O/g dry extract) assays. TCH4 extract exhibited cytotoxic activity in the DU-145 cell line (IC50 9.36?}0.37 ?g/ mL). Analysis of extract constituents by GC-MS revealed pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl) (36.85%), benzeneacetamide (23.76%), and deferoxamine (12.85%) as the major compounds, which have been reported to possess pharmaceutical properties. S. achromogenes TCH4 could be a potential source of bioactive metabolites with antibacterial, antioxidant and anticancer activities for pharmaceutical applications.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68388773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Chidamide modulates proliferation, migration and apoptosis of human tongue squamous carcinoma SCC9 cells through multiple signaling pathways 奇达胺通过多种信号通路调控人舌鳞癌SCC9细胞的增殖、迁移和凋亡

IF 0.8 4区生物学

Archives of Biological Sciences Pub Date : 2021-01-01 DOI: 10.2298/abs210815035h

Hong-jie Huang, T. Deng, Jin Qian, Jie Hu, Yangyang Zhu, M. Tian, Xiaohong Guo, Lili Lu

{"title":"Chidamide modulates proliferation, migration and apoptosis of human tongue squamous carcinoma SCC9 cells through multiple signaling pathways","authors":"Hong-jie Huang, T. Deng, Jin Qian, Jie Hu, Yangyang Zhu, M. Tian, Xiaohong Guo, Lili Lu","doi":"10.2298/abs210815035h","DOIUrl":"https://doi.org/10.2298/abs210815035h","url":null,"abstract":"Chidamide, a histone deacetylase (HDAC) inhibitor, displays antitumor activities in different tumor cells. Tongue squamous cell carcinoma (TSCC) is the most prevalent oral cavity malignancy with a high incidence and a high mortality rate. We describe the antitumor effects of chidamide on human TSCC SCC9 cells, which has not been reported before. Cell viability and wound healing assay and flow cytometry analysis were used to determine the proliferation, migration, cell cycle and apoptosis of chidamide-treated SCC9 cells in vitro. Western blotting was used to detect relative changes in protein levels. Our results reveal that chidamide inhibits SCC9 cell proliferation by decreasing ERK1/2 and mTOR phosphorylation and arresting the cell cycle in G0/G1 phase. Chidamide decreased cell migration in dose- and time-dependent manner by increasing E-cadherin expression. Chidamide induced SCC9 cells apoptosis by increasing the level of cleaved caspase-3 and decreasing the expression of Bcl-2. To sum up, chidamide displayed potent antitumor effects on SCC9 cells through multiple signaling pathways and has the potential to be developed as a new therapeutic agent to treat TSCC.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68389684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0