Archives of Biological Sciences最新文献_第8页

Assessment of the impact of copper mining and related industrial activities on aquatic macroinvertebrate communities of the Pek River (Serbia) 铜矿开采及相关工业活动对佩克河水生大型无脊椎动物群落的影响评估(塞尔维亚)

IF 0.8 4区生物学

Archives of Biological Sciences Pub Date : 2021-01-01 DOI: 10.2298/ABS210314023C

Jelena Canak-Atlagic, Stefan Andjus, Jelena Djuknic, Ana Milivojević, Katarina Kulic, Jovo Pokrajac, B. Tubic, M. Paunović

{"title":"Assessment of the impact of copper mining and related industrial activities on aquatic macroinvertebrate communities of the Pek River (Serbia)","authors":"Jelena Canak-Atlagic, Stefan Andjus, Jelena Djuknic, Ana Milivojević, Katarina Kulic, Jovo Pokrajac, B. Tubic, M. Paunović","doi":"10.2298/ABS210314023C","DOIUrl":"https://doi.org/10.2298/ABS210314023C","url":null,"abstract":"The upper stretch of the Pek River is located in proximity to one of the largest mining sites in Serbia. To estimate the influence of copper mining on river biota, the quantitative and qualitative macroinvertebrate community structure was analyzed, and the ecological status of the river was assessed using standard biological indices. Data was gathered at 6 sites along the river. A total of 75 macroinvertebrate taxa were recorded. The reference site had the highest number of recorded taxa (48) and a high biodiversity (diversity index H=2.3). In contrast, the lowest number of taxa (8), a low diversity index value (H=1.15), and a very poor overall ecological status were recorded at site 2, which is heavily influenced by mining discharge. The Ephemeroptera, Plecoptera and Trichoptera group (EPT) constituted 46.30% of the community at site 1 but was completely absent at site 2 where Diptera (70%) and Oligochaeta (20%) were dominant, reflecting a strong impact of the mining industry on the upper river stretch. A gradual improvement of water quality downstream of the pollution sources indicates that this medium-sized river has the capacity to overcome intensive pollution and to revitalize itself along its course.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68388694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Protective effects of pumpkin (Cucurbita pepo L.) seed oil on rat liver damage induced by chronic alcohol consumption 南瓜籽油对慢性酒精所致大鼠肝损伤的保护作用

IF 0.8 4区生物学

Archives of Biological Sciences Pub Date : 2021-01-01 DOI: 10.2298/ABS201205008R

I. Radic, M. Miric, M. Mijović, N. Tatalović, M. Mitić, V. Nestorovic, M. Adzic, D. Blagojević, L. Popović, S. Janićijević-Hudomal

{"title":"Protective effects of pumpkin (Cucurbita pepo L.) seed oil on rat liver damage induced by chronic alcohol consumption","authors":"I. Radic, M. Miric, M. Mijović, N. Tatalović, M. Mitić, V. Nestorovic, M. Adzic, D. Blagojević, L. Popović, S. Janićijević-Hudomal","doi":"10.2298/ABS201205008R","DOIUrl":"https://doi.org/10.2298/ABS201205008R","url":null,"abstract":"Pumpkin seed oil (PSO) possesses a protective potential against liver injury due to the presence of biologically active ingredients. Adult male albino rats were administrated PSO (per os, 2 mL/kg b.w./day) and a 12% ethanol solution in water, ad libitum, with an average intake of 8.14 g of ethanol/kg bw/day for 6 weeks. Congestion, hepatic central vein dilation, portal vein branch dilation, Kupffer cell hyperplasia, fatty liver changes, hepatocyte focal necrosis were observed after daily alcohol intake. All observed changes were reduced when PSO was ingested with ethanol. PSO intake itself induced discrete cellular edema, congestion and slight dilatation of the central and portal vain branches. Chronic ethanol intake elevated catalase (CAT) activity and glutathione reductase (GR) protein expression; concomitant PSO intake had no effect on CAT activity or GR protein expression. PSO intake decreased the activities of GR, glutathione-S-transferase (GST) and xanthine oxidase (XOD) in the liver, probably due to the ingestion of antioxidants. Intake of PSO and ethanol significantly decreased cytosolic superoxide dismutase (SOD1) and increased NF-?B protein expression compared to ethanol intake, suggesting that the protective effects of PSO were mediated by the NF-?B signaling pathway. Our results reveal a therapeutic potential of PSO in alcoholic liver disease.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68388791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Interleukin-17 modulates uPA and MMP2 expression in human periodontal ligament mesenchymal stem cells: Involvement of the ERK1/2 MAPK pathway 白细胞介素-17调节人牙周韧带间充质干细胞中uPA和MMP2的表达:ERK1/2 MAPK通路的参与

IF 0.8 4区生物学

Archives of Biological Sciences Pub Date : 2021-01-01 DOI: 10.2298/abs210929048o

I. Okic-Djordjevic, T. Kukolj, Hristina Obradović, D. Trivanović, Andjelija Petrovic, S. Mojsilović, M. Miletic, D. Bugarski, A. Jauković

{"title":"Interleukin-17 modulates uPA and MMP2 expression in human periodontal ligament mesenchymal stem cells: Involvement of the ERK1/2 MAPK pathway","authors":"I. Okic-Djordjevic, T. Kukolj, Hristina Obradović, D. Trivanović, Andjelija Petrovic, S. Mojsilović, M. Miletic, D. Bugarski, A. Jauković","doi":"10.2298/abs210929048o","DOIUrl":"https://doi.org/10.2298/abs210929048o","url":null,"abstract":"Periodontal disease is a chronic infection of periodontal tissue characterized by extracellular matrix (ECM) degradation due to increased expression of plasminogen activators and matrix metalloproteinases (MMPs) and various proinflammatory cytokines, including interleukin (IL)-17. Successful regeneration of damaged periodontal tissues depends on the proper functionality of periodontal ligament mesenchymal stem cells (PDLMSCs), especially the production of extracellular matrix proteases. We investigated the influence of IL-17 on ECM remodeling through modulation of urokinasetype plasminogen activator (uPA) and MMP2/MMP9 expression in human PDLMSCs at mRNA, protein and activity levels using by RT-PCR, Western blotting and zymography, respectively. Investigation of the involvement of MAPKs in these processes in PDLMSCs was determined by Western blotting, as well as by utilizing specific p38 and MEK1/2 inhibitors. Our results show that IL-17 activates MAPK signaling in PDLMSCs. Moreover, IL-17 had no effect on MMP9 expression, but it stimulated uPA and MMP2 gene and protein expression in PDLMSCs through the activation of the ERK1/2 MAPK signaling pathway. The obtained data suggest that IL-17 contributes to ECM degradation in the periodontal ligament by stimulating uPA and MMP2 expression and activity in PDLMSCs. This information is important for understanding periodontal disease development and defining future directions of its treatment.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68389859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Aqueous extract from Chrysophyllum cainito bark exhibits embryonic toxicity in Danio rerio and negligible acute toxicity in adult wistar rats 金菊树皮水提物对小鼠具有胚胎毒性，对成年wistar大鼠具有可忽略的急性毒性

IF 0.8 4区生物学

Archives of Biological Sciences Pub Date : 2021-01-01 DOI: 10.2298/abs211110046d

Hau V Doan, P. Sritangos, O. Weeranantanapan, N. Chudapongse

{"title":"Aqueous extract from Chrysophyllum cainito bark exhibits embryonic toxicity in Danio rerio and negligible acute toxicity in adult wistar rats","authors":"Hau V Doan, P. Sritangos, O. Weeranantanapan, N. Chudapongse","doi":"10.2298/abs211110046d","DOIUrl":"https://doi.org/10.2298/abs211110046d","url":null,"abstract":"Chrysophyllum cainito has been used as a traditional medicine to treat a wide range of diseases, but the toxicity profile of this plant remains unknown. This study aimed to evaluate the acute toxicity of the aqueous extract of C. cainito (CE) bark based on OECD guidelines in two different in vivo experimental models: acute single-dose oral toxicity in adult Wistar rats and the zebrafish embryo acute toxicity test. All concentrations of CE (500-4000 mg/kg) tested during a 14-day period in both male and female rats showed no effect on behavior, body weight, organ weights, biochemical and hematological parameters. In contrast, CE significantly delayed zebrafish embryo hatching and decreased embryo survival rates in a dose-dependent manner. Hatched larvae were notably sensitive to CE-induced toxicity compared to unhatched fish embryos. Acridine orange staining showed that CE induced apoptosis in the yolk sac region that is responsible for supplying nutrients to support larval growth and development. According to OECD guidelines, CE was identified as GHS category 5, a substance with low to no acute toxicity. However, as embryotoxicity was observed in zebrafish, CE use during pregnancy should be exercised with caution until further examination of its safety.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68389902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

One-step purification and freeze stability of papain at acidic pH values 木瓜蛋白酶在酸性pH值下的一步纯化和冷冻稳定性

IF 0.8 4区生物学

Archives of Biological Sciences Pub Date : 2021-01-01 DOI: 10.2298/ABS201217001M

S. Marković, J. Milošević, Milica D. Djurić, A. Lolić, N. Polović

引用次数: 2

Agmatine reduces chlorpromazine prooxidant effects in rat hippocampus and striatum 胍丁胺降低氯丙嗪在大鼠海马和纹状体中的抗氧化作用

IF 0.8 4区生物学

Archives of Biological Sciences Pub Date : 2021-01-01 DOI: 10.2298/abs210429028d

Bratislav Dejanović, Vesna Begović-Kuprešanin, I. Stevanović, I. Lavrnja, B. Šošić-Jurjević, M. Ninković, S. Trifunović

{"title":"Agmatine reduces chlorpromazine prooxidant effects in rat hippocampus and striatum","authors":"Bratislav Dejanović, Vesna Begović-Kuprešanin, I. Stevanović, I. Lavrnja, B. Šošić-Jurjević, M. Ninković, S. Trifunović","doi":"10.2298/abs210429028d","DOIUrl":"https://doi.org/10.2298/abs210429028d","url":null,"abstract":"The use of the antidepressant drug chlorpromazine (CPZ) is linked to the occurrence of oxidative stress in some brain structures. Thus, overcoming the side effects of CPZ is of great importance. Because agmatine (AGM) can act as a free radical scavenger, it is an interesting compound as an adjunct to CPZ therapy. The aim of our study was to investigate the enzymatic parameters of oxidative stress in the hippocampus and striatum of rats after CPZ treatment, and the potential protective effects of AGM. Rats were injected as follows with (i) 1 mL/kg b.w. saline; (ii) a single intraperitoneal (i.p.) dose of CPZ (38.7 mg/kg); (iii) CPZ (38.7 mg/kg) and AGM (75 mg/kg); (iv) AGM (75 mg/kg). CPZ induced an increase in superoxide anion radical (O2 ?-) concentration, while the activities of the antioxidant enzymes, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR), were lowered in both the hippocampus and striatum. Cotreatment with CPZ and AGM protected the examined brain structures by reversing the antioxidant enzyme activities to the control values. Following CPZ treatment, the effects were more pronounced for SOD and GPx in the hippocampus, and for SOD, CAT and GPx in the striatum. The full effect of restored superoxide production was achieved in the striatum, which points to the role of CAT. The obtained results suggest that CPZ in combination with AGM may be considered as a new treatment strategy.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68388980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neural pathways underlying the interplay between emotional experience and behavior, from old theories to modern insight 情绪体验和行为之间相互作用的神经通路，从古老的理论到现代的见解

IF 0.8 4区生物学

Archives of Biological Sciences Pub Date : 2021-01-01 DOI: 10.2298/abs210510029s

O. Stanojlović, N. Šutulović, D. Hrnčić, D. Mladenović, A. Rašić-Marković, Nebojsa Randunovic, Milena Vesković

{"title":"Neural pathways underlying the interplay between emotional experience and behavior, from old theories to modern insight","authors":"O. Stanojlović, N. Šutulović, D. Hrnčić, D. Mladenović, A. Rašić-Marković, Nebojsa Randunovic, Milena Vesković","doi":"10.2298/abs210510029s","DOIUrl":"https://doi.org/10.2298/abs210510029s","url":null,"abstract":"Emotions are specific psychological states brought about by neurophysiological changes associated with feelings, thoughts and behavioral responses. Emotions were considered as irrational experiences beyond the domain of logical perception because of their intertwinement with mood, temperament, creativity, motivation and personality. Through the centuries, emotions have been the focus of research among great classical philosophers, doctors, neuropsychologists, neuroscientists, neurologists and psychiatrists. The neurophysiological basis of behavior, such as emotional facial expression, and autonomic events in the physiological theory of William James and James-Lange and modified by Cannon-Bard, was followed by the two-factor theory of emotions of Schachter-Singer and Lazarus? higher-order cognitive evaluation. Four components that influence each other represent the concept of emotions and complete the overall emotional experience, and these are: autonomous (increase in heart rate, blood pressure); somatic (body language, facial expressions); cognitive (control, management), and subjective feeling (emotion, individual experience). The interplay between emotions and cognition has been the subject of research. Emotions can be evoked reflexively by simple physical stimuli (bottom-up), but can also be complex reactions involving cognitive, physiological and behavioral reactions (top-down). The amygdala, the ?alert\" or ?neural alarm? structure, is responsible for conditioning fear, while the medial prefrontal cortex participates in emotion self-regulation and decision making.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68388992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Diet of the European ground squirrel (Spermophilus citellus) in the southern Pannonian plain 潘诺尼亚平原南部欧洲地松鼠的饮食

IF 0.8 4区生物学

Archives of Biological Sciences Pub Date : 2021-01-01 DOI: 10.2298/ABS201231007A

Maja Arok, T. Nikolić, Barbara Győri-Koósz, Lea Milinski, D. Ćirović

{"title":"Diet of the European ground squirrel (Spermophilus citellus) in the southern Pannonian plain","authors":"Maja Arok, T. Nikolić, Barbara Győri-Koósz, Lea Milinski, D. Ćirović","doi":"10.2298/ABS201231007A","DOIUrl":"https://doi.org/10.2298/ABS201231007A","url":null,"abstract":"Overwinter survival of hibernators is directly influenced by the fat reserves accumulated during the active season. The European ground squirrel, Spermophilus citellus, an endangered flagship species of the open grasslands of central and southeastern Europe, is no exception. Considering anthropogenic changes affecting its habitats in northern Serbia, we studied the species? feeding habits by subjecting its feces to microhistological examination. In addition to identifying plant remains in the feces, we examined the vegetation of the four study sites located inside a local mosaic of steppe and saline pastures. Our findings indicate that these remaining European ground-squirrel sites are diverse and stable grasslands covered by native flora that represents a complete dietary base for the species. The diet is dominated by a small number of plant species, specifically Achillea millefolium, legumes and grasses, with sporadic contributions from a number of other species. The diet of S. citellus also differs by location and season in that grasses dominate during the early season, while A. millefolium takes precedence later in the year. This dietary information is essential for the development of future species and habitat management strategies. In light of the predicted impact of climate change on grasslands in northern Serbia, our findings will be instrumental for translocations and conservation actions in the future.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68389022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Correlation between echocardiographic findings and biochemical markers in term newborns with moderate to severe perinatal asphyxia 中重度围产期窒息足月新生儿超声心动图表现与生化指标的相关性

IF 0.8 4区生物学

Archives of Biological Sciences Pub Date : 2021-01-01 DOI: 10.2298/ABS210121012V

M. Vasiljevic, D. Nešić, Stasa Krasic, Sanja Ninic, S. Prijic, M. Ašanin, S. Stanković, Ivana Cerovic, S. Milićević, Sanja Knezevic-Rangelov, V. Vukomanovic

{"title":"Correlation between echocardiographic findings and biochemical markers in term newborns with moderate to severe perinatal asphyxia","authors":"M. Vasiljevic, D. Nešić, Stasa Krasic, Sanja Ninic, S. Prijic, M. Ašanin, S. Stanković, Ivana Cerovic, S. Milićević, Sanja Knezevic-Rangelov, V. Vukomanovic","doi":"10.2298/ABS210121012V","DOIUrl":"https://doi.org/10.2298/ABS210121012V","url":null,"abstract":"Perinatal asphyxia (PA) is a condition characterized by a gas exchange disorder due to a lack of blood flow or gas exchange, with potential multiorgan dysfunction. Our study aimed to determine the correlation between biochemical markers and echocardiography findings in a group of asphyxiated newborns. The prospective cohort study included 120 neonates (52/120 with PA) treated at a tertial referral pediatric center, from 2012 to 2014. A moderate-strong positive correlation was recorded between the transtricuspid pressure gradient (TRPG) and serum lactate, and between TRPG and NT-proBNP in the PA group (P<0.001) on the 1st day of life. A moderate positive correlation was found between NT-proBNP, lactate and troponins on one side, and TRPG on the other in the PA group after the 2nd measurement. Multinomial regression analysis showed that the lactate level was an independent factor for survival on the 1st (odds ratio (OR) 41.3, 95% confidence interval (CI) 2.14-797.1) and 3rd (OR 136.4, 95% CI 2.27-8206.7) days. Our research confirmed a significant correlation between echocardiographic and biochemical parameters of the myocardial lesion and cardiac function. Due to their complementarity, the use of the biochemical and echocardiographic parameters may be conditioned by their availability.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68389064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of two novel type II topoisomerase mutations in Enterococcus spp. isolated from a hospital in China 中国某医院分离肠球菌两种新型II型拓扑异构酶突变的鉴定

IF 0.8 4区生物学

Archives of Biological Sciences Pub Date : 2021-01-01 DOI: 10.2298/abs210628034s

R. Su, Yunzhi Peng, Zhanli Wang, Hui Yu, Qi Wu

{"title":"Identification of two novel type II topoisomerase mutations in Enterococcus spp. isolated from a hospital in China","authors":"R. Su, Yunzhi Peng, Zhanli Wang, Hui Yu, Qi Wu","doi":"10.2298/abs210628034s","DOIUrl":"https://doi.org/10.2298/abs210628034s","url":null,"abstract":"Type II topoisomerases, including DNA gyrase (GyrA) and topoisomerase IV (ParC), contribute to fluoroquinolone resistance in Enterococcus spp. This study investigated the mutational status of the quinolone resistance-determining regions (QRDRs) of GyrA and ParC in the clinical isolates of enterococci from a hospital in Baotou, China. We analyzed 110 enterococcal isolates, including 57 Enterococcus faecalis and 53 Enterococcus faecalis faecium. The resistance rates of E. faecalis and E. faecium to ciprofloxacin were 63.16% and 84.91%, respectively. We found that 32 samples of E. faecalis and 42 of E. faecium had single or combined mutations in gyrA and/or parC, which were all resistant to ciprofloxacin. Only two ciprofloxacin-resistant E. faecalis isolates had no mutation. No mutations in gyrA and parC genes in all ciprofloxacinsusceptible isolates were found. Ciprofloxacin minimal inhibitory concentrations (MICs) in the mutation group were significantly higher than those of the non-mutation group, indicating that mutations in the QRDRs of gyrA and parC were correlated with MIC elevation. Two novel substitutions (GyrA Ser83Phe and ParC Ser80Leu) of E. faecalis were identified herein. Three-dimensional modeling revealed that these novel amino acid substitutions could disrupt the water/metal-ion bridge and decrease the interaction between the enzymes and ciprofloxacin. The data showed a diversity of mutation types in QRDRs of type II topoisomerases whose association with fluoroquinolone resistance in clinical isolates of enterococci warrants further investigation.","PeriodicalId":8145,"journal":{"name":"Archives of Biological Sciences","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68389610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0