Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes最新文献_第3页

Establishment 5 doses presentation of the triple viral vaccine without albumin to reduce loss during administration 建立5剂不含白蛋白的三联病毒疫苗，以减少给药过程中的损失

Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes Pub Date : 2022-01-01 DOI: 10.35259/isi.2022_52264

S. Assumpção, M. Freire, E. Caride, C. Crespo

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Generation and characterization of anti-CD19 CAR-T cells overexpressing the protein PHF19 过表达PHF19蛋白的抗cd19 CAR-T细胞的产生和表征

Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes Pub Date : 2022-01-01 DOI: 10.35259/isi.2022_52280

K. Hajdu, Leonardo Silva, M. Bonamino

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Optimizing usage of remaining blood from Interferon Gamma Releasing Assay (IGRA) to search for immune signatures and biomarkers for Latent Tuberculosis Infection (LTBI) 优化干扰素γ释放试验(IGRA)剩余血液的使用，寻找潜伏性结核感染(LTBI)的免疫特征和生物标志物

Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes Pub Date : 2022-01-01 DOI: 10.35259/isi.2022_52179

B. Ferreira, M. Nunes, D. Rodrigues, P. Rigato

{"title":"Optimizing usage of remaining blood from Interferon Gamma Releasing Assay (IGRA) to search for immune signatures and biomarkers for Latent Tuberculosis Infection (LTBI)","authors":"B. Ferreira, M. Nunes, D. Rodrigues, P. Rigato","doi":"10.35259/isi.2022_52179","DOIUrl":"https://doi.org/10.35259/isi.2022_52179","url":null,"abstract":"of T cell activation molecules IFN-g production in response to QTF We immunophenotyped the remaining blood cells used in the QTF test. Our preliminary results show that, as expected, most of the cells present in the lymphocyte gate are CD45+/CD3+ with a median of 77.8% (Tube N, Nill); 82.5% (Mitogen, M); 74.03% (TB1) and 76.75% (TB2). Considering CD4+ T cells, we detected 56% (N), 44% (M), 56% (TB1) and 55% (TB2). On the other hand, we showed that there were 28% (N), 25% (M), 31% (TB1) and 29% (TB2) of CD8+ T cells in the tubes. When we looked for activation markers (CD69, CD71 and HLA-DR) we showed that when T cells are stimulated with mitogen there are higher percentages of T cells, TCD4+ TCD8+ expressing these markers. For the antigen-specific tubes (TB1 and TB2) there were not enough QTF positive samples to conclude the correlation of IFN-g production with the expression of these activation markers. Conclusion: We conclude that it is possible to better characterize the immune phenotype of remaining blood from IGRA.","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83297598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Continued Process Verification for COVID-19 Vaccine Formulation and Packaging (recombinant) COVID-19疫苗制剂和包装(重组)继续工艺验证

Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes Pub Date : 2022-01-01 DOI: 10.35259/isi.2022_52269

Paulo Silva, Pedro Correia, Daiane Grugel, Victor Ferreira, Rayane Gonçalves

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Technological roadmap in covid-19 vaccine production covid-19疫苗生产技术路线图

Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes Pub Date : 2022-01-01 DOI: 10.35259/isi.2022_52278

Silvia Santos, Wanise Barroso

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Scale up of molecular kit for diagnostic of COVID-19 during pandemic period 在大流行期间扩大用于诊断COVID-19的分子试剂盒规模

Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes Pub Date : 2022-01-01 DOI: 10.35259/isi.2022_52178

Carla Almeida, M. Stávale, Fernanda Santos, Patricia Baptista

引用次数: 0

A significant portion of undergraduate health science students are not immunized as they should 相当一部分健康科学本科学生没有接种他们应该接种的疫苗

Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes Pub Date : 2022-01-01 DOI: 10.35259/isi.2022_52217

C. Vitral, Samuel Machado, Camila Santos, E. Pinto, Leonardo Abreu

引用次数: 0

Evaluation of a Wuchereria bancrofti recombinant antigen for the capture antibody diagnosis of dog heartworm 一种重组班氏乌切利氏菌抗原对犬心丝虫捕获抗体诊断的评价

Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes Pub Date : 2022-01-01 DOI: 10.35259/isi.2022_52182

Darleide Correia, Esdras Gueiros, Wagner J T Santos, A. Rocha, L. Alves

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Screening system development for HDAC1/Sp1 complex inhibitors HDAC1/Sp1复合物抑制剂筛选系统的开发

Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes Pub Date : 2022-01-01 DOI: 10.35259/isi.2022_52294

F. Verza, Ana Saltoratto, M. Marins

{"title":"Screening system development for HDAC1/Sp1 complex inhibitors","authors":"F. Verza, Ana Saltoratto, M. Marins","doi":"10.35259/isi.2022_52294","DOIUrl":"https://doi.org/10.35259/isi.2022_52294","url":null,"abstract":"gene expression regulators. expression on this to target the elements that interact with Sp1 such as histone deacetylase 1 (HDAC1). The interaction of its domain and the C-terminal region of Sp1 inhibits the connection of HDAC1 with the promoter region of genes and repress gene expression. Objective: Considering the role of post-translational modifications in determining the transcriptional activity of Sp1 and the interaction with other proteins such as HDAC1, we report a system for the development of inhibitors of the HDAC1/Sp1 complex using mammalian two-hybrid system. Methodology: In this strategic approach, the DNA-binding domain and the transcriptional activation domain are produced by separate plasmids and become closely associated when the protein HDAC1 fused to a DNA-binding domain, interacts with the protein Sp1 fused to a transcriptional activation domain. The interaction between the proteins results in transcription of the firefly luciferase reporter gene. Results: With this experimental system we can select substances that inhibit the HDAC1/Sp1 interaction and use them in the development of anticancer drugs based on the activation of tumor suppressor genes regulated by Sp1/HDAC1 complex. Conclusion: Our system is applicable to the screening of HDAC1/Sp1 binding inhibitors to assess their antitumor and toxicity activity, but due to the complexity of histone modifications and transcriptional initiation, we cannot rule out the involvement of other epigenetic enzymes or transcription factors.","PeriodicalId":8089,"journal":{"name":"Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89190497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In silico identification of epitopes target of humoral response against Sphingomyelinase 2 (Sph2) of pathogenic Leptospira 致病性钩端螺旋体鞘磷脂酶2 (Sph2)体液应答表位靶点的计算机识别

Annals of the symposium: vaccines, biopharmaceuticals, in vitro diagnosis, management, other related themes Pub Date : 2022-01-01 DOI: 10.35259/isi.2022_52218

Laura Ataídes, Fernanda Maia, F. Conte, Rodrigo Silva

引用次数: 0