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The integrative nature of biochemistry: challenges of biochemical education in the USA. 生物化学的整体性:美国生物化学教育的挑战。
Biochemical education Pub Date : 2000-03-01
Huang
{"title":"The integrative nature of biochemistry: challenges of biochemical education in the USA.","authors":"Huang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The intricate interplay of Biochemistry with well-established disciplines often blurs the identity of the subject. The issues are many. What is biochemical education? Who should be educated? What should be taught? What should the requirements be for Biochemistry major? What is a career in Biochemistry? The curriculum, course syllabus and application of Biochemistry are ever-evolving concerns. The challenges are particularly keen in the USA due to the diversity in its teaching modes, and in the composition of the student body. The constant changes in technologies also shift the needs of skill and knowledge of the graduates. This presentation is to examine the Biochemistry degree programs in the USA, particularly the curricula of private and public research universities, contrasting them with those of the liberal arts colleges. The goal is to sense the trends of changes, probing how the challenges are met, and to solicit and formulate recommendations as we approach a new millennium.</p>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 2","pages":"64-70"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21575529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An experiment on apoptosis induced by polyamine adducts produced in the presence of serum amine oxidase 血清胺氧化酶存在下多胺加合物诱导细胞凋亡的实验
Biochemical education Pub Date : 2000-03-01 DOI: 10.1016/S0307-4412(99)00125-9
Ignacio Fajardo, José Luis Urdiales, Francisca Sánchez-Jiménez, Miguel Ángel Medina
{"title":"An experiment on apoptosis induced by polyamine adducts produced in the presence of serum amine oxidase","authors":"Ignacio Fajardo,&nbsp;José Luis Urdiales,&nbsp;Francisca Sánchez-Jiménez,&nbsp;Miguel Ángel Medina","doi":"10.1016/S0307-4412(99)00125-9","DOIUrl":"https://doi.org/10.1016/S0307-4412(99)00125-9","url":null,"abstract":"<div><p>A two-session experiment is proposed to train students with an easy, economic and educative procedure to detect the typical DNA ladder produced in many apoptotic events. The procedure is accurate enough to provide an easy way to compare degrees of damage in DNA caused by different treatments.</p></div>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 2","pages":"Pages 110-112"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(99)00125-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137001108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Chemical cross-linking analysis of Ca2+-ATPase from rabbit skeletal muscle 兔骨骼肌Ca2+- atp酶的化学交联分析
Biochemical education Pub Date : 2000-01-01 DOI: 10.1016/S0307-4412(98)00268-4
Brendan E. Murray, Kay Ohlendieck
{"title":"Chemical cross-linking analysis of Ca2+-ATPase from rabbit skeletal muscle","authors":"Brendan E. Murray,&nbsp;Kay Ohlendieck","doi":"10.1016/S0307-4412(98)00268-4","DOIUrl":"https://doi.org/10.1016/S0307-4412(98)00268-4","url":null,"abstract":"<div><p>A student laboratory experiment is described dealing with chemical cross-linking analysis of Ca<sup>2+-</sup>ATPase from rabbit muscle. The experiment introduces students to a wide range of techniques including subcellular fractionation, chemical cross-linking, polyacrylamide gel electrophoresis, blotting and immunochemical detection of proteins.</p></div>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 1","pages":"Pages 41-46"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(98)00268-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136412178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Classifying amino acids as gluco(glyco)genic, ketogenic, or both 将氨基酸分为葡萄糖原、生酮原或两者兼而有之
Biochemical education Pub Date : 2000-01-01 DOI: 10.1016/S0307-4412(98)00271-4
Gabriele D’Andrea
{"title":"Classifying amino acids as gluco(glyco)genic, ketogenic, or both","authors":"Gabriele D’Andrea","doi":"10.1016/S0307-4412(98)00271-4","DOIUrl":"10.1016/S0307-4412(98)00271-4","url":null,"abstract":"<div><p>Eleven popular and recent biochemistry textbooks were examined and compared concerning the classification of amino acids as being gluco(glyco)genic, ketogenic, or both. The main differences concerned mostly two amino acids, threonine, which in some cases is considered only gluco(glyco)genic and in others both gluco(glyco)-ketogenic, and lysine, which is considered chiefly ketogenic, but in two cases is reported as being gluco(glyco)-ketogenic. Some more mistakes, discrepancies and curiosities among different textbooks are also reported. Biochemistry textbook authors and teachers should be aware of this situation to avoid any confusion, especially among students.</p></div>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 1","pages":"Pages 27-28"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(98)00271-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21569896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 12
Problem-based learning in veterinary medicine: protein metabolism. 基于问题的兽医学学习:蛋白质代谢。
Biochemical education Pub Date : 2000-01-01
Rivarola, García
{"title":"Problem-based learning in veterinary medicine: protein metabolism.","authors":"Rivarola,&nbsp;García","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A problem-based approach to teaching biochemistry to veterinary students is described which aims to encourage the finding and learning of biochemical information that is relevant to real-life veterinary problems.</p>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 1","pages":"30-31"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21569897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of biotechnology education in Turkey 土耳其生物技术教育的发展
Biochemical education Pub Date : 2000-01-01 DOI: 10.1016/S0307-4412(99)00116-8
Feride Severcan , Aysegul Ozan , Parvez I. Haris
{"title":"Development of biotechnology education in Turkey","authors":"Feride Severcan ,&nbsp;Aysegul Ozan ,&nbsp;Parvez I. Haris","doi":"10.1016/S0307-4412(99)00116-8","DOIUrl":"10.1016/S0307-4412(99)00116-8","url":null,"abstract":"<div><p>For sometime Turkish scientists have been actively involved in biotechnology related research. However, biotechnology educa-tion in Turkey is a relatively recent phenomenon. The subject has not been addressed at the undergraduate level in a serious way until recently. This is evident from the lack of undergraduate degree programmes in biotechnology at Turkish Universities. The Turkish scientific establishment is very much aware of the importance of biotechnology and has identified this subject as one of the priority areas. The Universities are taking positive steps towards enhancing Biotechnology education. This article focuses on the emergence, as well as the problems and prospects of Biotechnology education in Turkey.</p></div>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 1","pages":"Pages 36-38"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(99)00116-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21569899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 12
Optimization of energy coupling: what is all the argument about? 能量耦合的优化：所有的争论都是关于什么的？
Biochemical education Pub Date : 2000-01-01 DOI: 10.1016/S0307-4412(99)00087-4
Keith L. Manchester
{"title":"Optimization of energy coupling: what is all the argument about?","authors":"Keith L. Manchester","doi":"10.1016/S0307-4412(99)00087-4","DOIUrl":"https://doi.org/10.1016/S0307-4412(99)00087-4","url":null,"abstract":"<div><p>Although it may be asked how effective glycolysis is in retaining the chemical energy in the bonds of glucose during its breakdown in the formation of ATP, the reasons for the coupled pathway of glycolysis having evolved as it has are probably as much as a consequence of the need to find reactions that can lead to formation of phosphoryl groups able to transfer to ADP as to the overall thermodynamics of the pathway. It is not meaningful to talk of optimization of energy coupling solely in terms of free energy changes.</p></div>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 1","pages":"Pages 18-19"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(99)00087-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72234578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Understanding ion transport by quantification of mitochondrial swelling 通过量化线粒体肿胀了解离子转运
Biochemical education Pub Date : 2000-01-01 DOI: 10.1016/S0307-4412(99)00095-3
Mina Königsberg
{"title":"Understanding ion transport by quantification of mitochondrial swelling","authors":"Mina Königsberg","doi":"10.1016/S0307-4412(99)00095-3","DOIUrl":"https://doi.org/10.1016/S0307-4412(99)00095-3","url":null,"abstract":"<div><p>Suspensions of mitochondria are turbid and scatter light. An increase in the matrix volume (swelling) due to the influx of permeable solutes results in a decrease in the amount of light scattered. This property can be used to study solute fluxes across the mitochondrial inner membrane. A rapid method for isolating mitochondria is presented along with three swelling experiments using energized and non-energized mitochondria to illustrate ion transport across energy transducing membranes.</p></div>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 1","pages":"Pages 24-26"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(99)00095-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137389685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A simple and rapid method for screening amylolytic bacteria 一种简单、快速筛选解淀粉细菌的方法
Biochemical education Pub Date : 2000-01-01 DOI: 10.1016/S0307-4412(99)00102-8
Pedro Chimoy Effio, Enny Fernandes Silva, Manuel Troyano Pueyo
{"title":"A simple and rapid method for screening amylolytic bacteria","authors":"Pedro Chimoy Effio,&nbsp;Enny Fernandes Silva,&nbsp;Manuel Troyano Pueyo","doi":"10.1016/S0307-4412(99)00102-8","DOIUrl":"https://doi.org/10.1016/S0307-4412(99)00102-8","url":null,"abstract":"<div><p>A laboratory class was designed for the study of the ecology of amylolytic bacteria in soil, although other sources may be equally suitable for this purpose. Groups of three students carried out the following: (a) preparation and sterilization of medium and plates, (b) collection and preparation of soil samples, spreading the samples on the plates, (c) incubation of the plates at 37°C overnight, a further 1 h incubation at 60°C to observe amylolytic activity due to thermophilic bacteria, and (d) interpretation and discussion of the results. These tasks are accomplished in two periods of 4<!--> <!-->h on consecutive days. No sophisticated instruments are required for these experiments, which can be carried out in three classes of 4<!--> <!-->h each. On the first day the students prepare culture media, buffers and reagents, as well as collect and grow soil samples. The second day is spent for both taxonomic identification of colonies and the HAI determination.</p></div>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 1","pages":"Pages 47-49"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(99)00102-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137389666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Does succinate oxidation yield FADH2 or ubiquinol? 琥珀酸氧化生成FADH2还是泛醇?
Biochemical education Pub Date : 2000-01-01 DOI: 10.1016/S0307-4412(99)00106-5
Simon Brown
{"title":"Does succinate oxidation yield FADH2 or ubiquinol?","authors":"Simon Brown","doi":"10.1016/S0307-4412(99)00106-5","DOIUrl":"https://doi.org/10.1016/S0307-4412(99)00106-5","url":null,"abstract":"<div><p>Most textbooks still show the oxidation of succinate in the tricarboxylic acid (TCA) cycle resulting in the reduction of FADH<sub>2</sub>. Such a presentation does not reflect the reaction catalysed by the enzyme in vivo or in vitro, does not simplify the treatment of the reaction, and is unnecessarily misleading and confusing.</p></div>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 1","pages":"Pages 52-54"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(99)00106-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137389682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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