发布求助
文献互助 智能选刊 最新文献

Biochemical education最新文献

筛选
英文 中文
Experimental treatment of the laws of heterogeneous catalysis with immobilized yeast cells (Saccharomyces cerevisiae) 固定化酵母细胞多相催化规律的实验处理
Biochemical education Pub Date : 2000-03-01 DOI: 10.1016/S0307-4412(99)00130-2
Peter Grunwald
{"title":"Experimental treatment of the laws of heterogeneous catalysis with immobilized yeast cells (Saccharomyces cerevisiae)","authors":"Peter Grunwald","doi":"10.1016/S0307-4412(99)00130-2","DOIUrl":"10.1016/S0307-4412(99)00130-2","url":null,"abstract":"<div><p>A series of simple, low-cost experiments is described in this paper that allows students to be introduced to some basic kinetic laws relating to heterogeneous catalysis. Immobilized yeast cells are used as the example and therefore simultaneously offer the opportunity to acquaint the students with the theoretical and practical background of an important branch of biotechnology.</p></div>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 2","pages":"Pages 96-99"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(99)00130-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56624022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
A guided experimental approach to practical molecular pharmacology teaching: demonstration of sequence selectivity of DNA-binding drugs by arrested polymerase chain reaction 分子药理学实践教学的实验指导方法:用阻滞聚合酶链反应演示dna结合药物的序列选择性
Biochemical education Pub Date : 2000-03-01 DOI: 10.1016/S0307-4412(99)00083-7
Roberto Gambari
{"title":"A guided experimental approach to practical molecular pharmacology teaching: demonstration of sequence selectivity of DNA-binding drugs by arrested polymerase chain reaction","authors":"Roberto Gambari","doi":"10.1016/S0307-4412(99)00083-7","DOIUrl":"10.1016/S0307-4412(99)00083-7","url":null,"abstract":"<div><p>Here, we report a simple experimental approach for demonstrating sequence-selectivity of DNA-binding drugs by using the polymerase-chain reaction (PCR). The experiments described could be easily and reproducibly performed by medical and science students and biochemistry teachers, do not require complex or expensive instruments and <sup>32</sup>P labelled probes or primers. The procedure described could represent a novel approach to practical biochemistry teaching in the field of biochemical pharma-cology.</p></div>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 2","pages":"Pages 100-103"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(99)00083-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56623549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
A single protein research integrated advanced biochemistry laboratory course; spectroscopic determination of tyrosyl side chain pKa 结合高等生物化学实验课程的单蛋白研究酪氨酸侧链pKa的光谱测定
Biochemical education Pub Date : 2000-03-01 DOI: 10.1016/S0307-4412(99)00127-2
Brian N. Blanchette, Bal Ram Singh
{"title":"A single protein research integrated advanced biochemistry laboratory course; spectroscopic determination of tyrosyl side chain pKa","authors":"Brian N. Blanchette,&nbsp;Bal Ram Singh","doi":"10.1016/S0307-4412(99)00127-2","DOIUrl":"https://doi.org/10.1016/S0307-4412(99)00127-2","url":null,"abstract":"<div><p>The unique bio-analytical properties of the amino acid tyrosine (Tyr) are the focus of this experiment from the research oriented biochemistry laboratory course at our university. In the present study p<em>K</em><sub>a<sub>1</sub></sub>, p<em>K</em><sub>a<sub>2</sub></sub>, and p<em>K</em><sub>a<sub>3</sub></sub> values for free Tyr were estimated to be 2.30, 9.40, and 9.97, respectively, when free Tyr was titrated with 1<!--> <!-->mM NaOH and 1<!--> <!-->mM HCl using a pH meter. Spectrophotometric analysis of the phenolic side chain p<em>K</em><sub>a<sub>3</sub></sub> revealed a value of 10.14, which was consistent with the p<em>K</em><sub>a</sub><em>s</em> estimated from the pH meter. The results from this experiment will allow students to compare the free Tyr properties with those present in a protein.</p></div>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 2","pages":"Pages 107-109"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(99)00127-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"137439794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A guided experimental approach to practical molecular pharmacology teaching: demonstration of sequence selectivity of DNA-binding drugs by arrested polymerase chain reaction. 分子药理学实践教学的实验指导方法:用阻滞聚合酶链反应演示dna结合药物的序列选择性。
Biochemical education Pub Date : 2000-03-01
Gambari
{"title":"A guided experimental approach to practical molecular pharmacology teaching: demonstration of sequence selectivity of DNA-binding drugs by arrested polymerase chain reaction.","authors":"Gambari","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Here, we report a simple experimental approach for demonstrating sequence-selectivity of DNA-binding drugs by using the polymerase-chain reaction (PCR). The experiments described could be easily and reproducibly performed by medical and science students and biochemistry teachers, do not require complex or expensive instruments and 32P labelled probes or primers. The procedure described could represent a novel approach to practical biochemistry teaching in the field of biochemical pharma-cology.</p>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 2","pages":"100-103"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21575436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Strategies for building criticism skills in undergraduate biochemists 生物化学本科生批评技巧的培养策略
Biochemical education Pub Date : 2000-03-01 DOI: 10.1016/S0307-4412(99)00138-7
Gareth Denyer
{"title":"Strategies for building criticism skills in undergraduate biochemists","authors":"Gareth Denyer","doi":"10.1016/S0307-4412(99)00138-7","DOIUrl":"10.1016/S0307-4412(99)00138-7","url":null,"abstract":"<div><p>This paper describes an easy method for empowering biochemistry students to think critically about research data. The technique encourages students to formulate their own opinions by removing the usually dominant ‘expert’ commentary. The exercise can be done individually or in very large groups, requires no specialist materials and confirmation that articulation and criticism skills have been learnt can be assessed under standard examination conditions. The skills learnt are not discipline specific and, as well as learning how best to read research papers, students also learn something about the research process.</p></div>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 2","pages":"Pages 74-75"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(99)00138-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21575531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Journal club as a supplement to the undergraduate biochemistry laboratory 期刊俱乐部作为本科生生物化学实验室的补充
Biochemical education Pub Date : 2000-03-01 DOI: 10.1016/S0307-4412(99)00135-1
Mona L. Hall, Adele J. Wolfson
{"title":"Journal club as a supplement to the undergraduate biochemistry laboratory","authors":"Mona L. Hall,&nbsp;Adele J. Wolfson","doi":"10.1016/S0307-4412(99)00135-1","DOIUrl":"https://doi.org/10.1016/S0307-4412(99)00135-1","url":null,"abstract":"<div><p>After purification of lysozyme, our biochemistry students write a research proposal that outlines a strategy for studying this enzyme after alteration by site-directed mutagenesis. Despite a literature search that yielded a wealth of background information, students were often overwhelmed by the assignment because they were not familiar with advanced techniques of protein analysis. We therefore developed a series of journal clubs in which teams of students present methods and data found in papers dealing with lysozyme. The five topics for journal clubs include; substrate binding and mechanism; spectroscopic techniques; stability analysis; two-dimensional NMR; and X-ray crystallography. After the adoption of the group talks, the quality of the research proposals improved immensely and students found the assignment to be an educationally rewarding exercise.</p></div>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 2","pages":"Pages 71-73"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(99)00135-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91634921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The excellence of turnip mitochondrial fraction. 萝卜线粒体分数的优良性。
Biochemical education Pub Date : 2000-03-01
Vicente, Madeira
{"title":"The excellence of turnip mitochondrial fraction.","authors":"Vicente,&nbsp;Madeira","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Several mitochondrial fractions were screened for suitability in practical experiments designed for students, with the following issues in mind: avoiding the use of animals; minimal expenditure of labour and time; high enzyme activities; accessible instruments and low-cost materials. Turnips and potato tubers were identified as the best materials from which to extract purified mitochondria according to these criteria, with high respiratory activities and integrity maintained during five consecutive days. Mitochondrial respiration was assayed for succinate, exogenous NADH, malate/pyruvate and alpha-ketoglutarate oxidations with excellent results. At the fifth day, the respiratory control was still about 3, the integrity of the outer mitochondrial membrane maintained at values higher than 80%, and the enzymes retained more than 55% of the initial activities.</p>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 2","pages":"104-106"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21575437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The excellence of turnip mitochondrial fraction. 萝卜线粒体分数的优良性。
Biochemical education Pub Date : 2000-03-01 DOI: 10.1111/J.1539-3429.2000.TB00034.X
Vicente, Madeira
{"title":"The excellence of turnip mitochondrial fraction.","authors":"Vicente, Madeira","doi":"10.1111/J.1539-3429.2000.TB00034.X","DOIUrl":"https://doi.org/10.1111/J.1539-3429.2000.TB00034.X","url":null,"abstract":"Several mitochondrial fractions were screened for suitability in practical experiments designed for students, with the following issues in mind: avoiding the use of animals; minimal expenditure of labour and time; high enzyme activities; accessible instruments and low-cost materials. Turnips and potato tubers were identified as the best materials from which to extract purified mitochondria according to these criteria, with high respiratory activities and integrity maintained during five consecutive days. Mitochondrial respiration was assayed for succinate, exogenous NADH, malate/pyruvate and alpha-ketoglutarate oxidations with excellent results. At the fifth day, the respiratory control was still about 3, the integrity of the outer mitochondrial membrane maintained at values higher than 80%, and the enzymes retained more than 55% of the initial activities.","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 2 1","pages":"104-106"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/J.1539-3429.2000.TB00034.X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63148007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
How do proteins fold? 蛋白质是如何折叠的?
Biochemical education Pub Date : 2000-03-01 DOI: 10.1016/S0307-4412(99)00144-2
Christopher A Smith
{"title":"How do proteins fold?","authors":"Christopher A Smith","doi":"10.1016/S0307-4412(99)00144-2","DOIUrl":"https://doi.org/10.1016/S0307-4412(99)00144-2","url":null,"abstract":"<div><p>This article emphasizes the importance of getting students to understand the ways in which polypeptides fold to form protein molecules with complex higher-ordered structures. Modern views on how this folding occurs in vitro and in the cell are summarized and set within an appropriate biological context.</p></div>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 2","pages":"Pages 76-79"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(99)00144-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90002194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A single protein research integrated advanced biochemistry laboratory course; spectroscopic determination of tyrosyl side chain pKa. 结合高等生物化学实验课程的单蛋白研究酪氨酸侧链pKa的光谱测定。
Biochemical education Pub Date : 2000-03-01
Blanchette, Singh
{"title":"A single protein research integrated advanced biochemistry laboratory course; spectroscopic determination of tyrosyl side chain pKa.","authors":"Blanchette,&nbsp;Singh","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The unique bio-analytical properties of the amino acid tyrosine (Tyr) are the focus of this experiment from the research oriented biochemistry laboratory course at our university. In the present study pK(a(1)), pK(a(2)), and pK(a(3)) values for free Tyr were estimated to be 2.30, 9.40, and 9.97, respectively, when free Tyr was titrated with 1mM NaOH and 1mM HCl using a pH meter. Spectrophotometric analysis of the phenolic side chain pK(a(3)) revealed a value of 10.14, which was consistent with the pK(a)s estimated from the pH meter. The results from this experiment will allow students to compare the free Tyr properties with those present in a protein.</p>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"28 2","pages":"107-109"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21575438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
首页 上一页 下一页 尾页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：481959085
Book学术官方微信