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A CAI System for Simulation of Enzymes Kinetics 酶动力学模拟CAI系统
Biochemical education Pub Date : 1999-10-01 DOI: 10.1016/S0307-4412(99)00070-9
G. G. Amorim, F. S. Leão, M. R. Martins, Joana D. R. Santos, M. D. Novaes, J. L. L. Filho
{"title":"A CAI System for Simulation of Enzymes Kinetics","authors":"G. G. Amorim, F. S. Leão, M. R. Martins, Joana D. R. Santos, M. D. Novaes, J. L. L. Filho","doi":"10.1016/S0307-4412(99)00070-9","DOIUrl":"https://doi.org/10.1016/S0307-4412(99)00070-9","url":null,"abstract":"","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"27 1","pages":"223-224"},"PeriodicalIF":0.0,"publicationDate":"1999-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(99)00070-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56622970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Bioconversion of waste paper materials to sugars: an application illustrating the environmental benefit of enzymes 废纸原料生物转化为糖:说明酶的环境效益的应用
Biochemical education Pub Date : 1999-10-01 DOI: 10.1016/S0307-4412(99)00053-9
J.P.H. van Wyk, M.A. Mogale, K.S. Moroka
{"title":"Bioconversion of waste paper materials to sugars: an application illustrating the environmental benefit of enzymes","authors":"J.P.H. van Wyk,&nbsp;M.A. Mogale,&nbsp;K.S. Moroka","doi":"10.1016/S0307-4412(99)00053-9","DOIUrl":"10.1016/S0307-4412(99)00053-9","url":null,"abstract":"<div><p>Recycling of waste materials into useful products is very topical not only to protect natural resources, but also to limit environmental pollution. The enzymatically catalyzed bioconversion of organic based waste such as used paper materials to soluble sugars like glucose can be performed in an environmental-friendly way. A basic experiment is proposed to show how different paper materials exhibit varying susceptibilities towards cellulase catalyzed saccharification, and that this approach can be applied by biochemistry or biotechnology students in the bioconversion of different cellulose related waste materials.</p></div>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"27 4","pages":"Pages 227-228"},"PeriodicalIF":0.0,"publicationDate":"1999-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(99)00053-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56622527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 11
Product review 产品评论
Biochemical education Pub Date : 1999-10-01 DOI: 10.1016/S0307-4412(99)00112-0
{"title":"Product review","authors":"","doi":"10.1016/S0307-4412(99)00112-0","DOIUrl":"10.1016/S0307-4412(99)00112-0","url":null,"abstract":"","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"27 4","pages":"Page 244"},"PeriodicalIF":0.0,"publicationDate":"1999-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(99)00112-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56623810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Sex determination using the polymerase chain reaction 用聚合酶链反应测定性别
Biochemical education Pub Date : 1999-10-01 DOI: 10.1016/S0307-4412(99)00044-8
Anthony S. Weiss, Jill M. Johnston
{"title":"Sex determination using the polymerase chain reaction","authors":"Anthony S. Weiss,&nbsp;Jill M. Johnston","doi":"10.1016/S0307-4412(99)00044-8","DOIUrl":"10.1016/S0307-4412(99)00044-8","url":null,"abstract":"<div><p>A practical class experiment on the PCR is described which has been used over several years as part of an undergraduate biochemistry and molecular biology course for science students. A major aim is to provide experience in the use of the polymerase chain reaction (PCR) and its interpretation. Students are given small coded DNA samples and use the PCR reaction to determine whether the sample is from a male or a female.</p></div>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"27 4","pages":"Pages 237-239"},"PeriodicalIF":0.0,"publicationDate":"1999-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(99)00044-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56622340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Product review 产品评论
Biochemical education Pub Date : 1999-10-01 DOI: 10.1016/S0307-4412(99)00070-9
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引用次数: 1
Index 指数
Biochemical education Pub Date : 1999-10-01 DOI: 10.1016/S0307-4412(99)00118-1
{"title":"Index","authors":"","doi":"10.1016/S0307-4412(99)00118-1","DOIUrl":"https://doi.org/10.1016/S0307-4412(99)00118-1","url":null,"abstract":"","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"27 4","pages":"Page 252"},"PeriodicalIF":0.0,"publicationDate":"1999-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(99)00118-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136812123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Product review 产品评论
Biochemical education Pub Date : 1999-10-01 DOI: 10.1016/S0307-4412(99)00063-1
{"title":"Product review","authors":"","doi":"10.1016/S0307-4412(99)00063-1","DOIUrl":"https://doi.org/10.1016/S0307-4412(99)00063-1","url":null,"abstract":"","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"27 4","pages":"Page 225"},"PeriodicalIF":0.0,"publicationDate":"1999-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(99)00063-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91720826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Second IUBMB workshop on biochemical education in Bucharest, Rumania, september 21–23, 1998 1998年9月21日至23日在罗马尼亚布加勒斯特举行的第二届生物化学教育讲习班
Biochemical education Pub Date : 1999-10-01 DOI: 10.1016/S0307-4412(99)00057-6
C. Sandu
{"title":"Second IUBMB workshop on biochemical education in Bucharest, Rumania, september 21–23, 1998","authors":"C. Sandu","doi":"10.1016/S0307-4412(99)00057-6","DOIUrl":"https://doi.org/10.1016/S0307-4412(99)00057-6","url":null,"abstract":"","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"27 1","pages":"192-192"},"PeriodicalIF":0.0,"publicationDate":"1999-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(99)00057-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56622924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
An experiment illustrating DNA-protein interactions using nuclear extracts from chicken erythrocytes 利用鸡红细胞核提取物说明dna -蛋白相互作用的实验
Biochemical education Pub Date : 1999-10-01 DOI: 10.1016/S0307-4412(99)00066-7
Beatriz Garat
{"title":"An experiment illustrating DNA-protein interactions using nuclear extracts from chicken erythrocytes","authors":"Beatriz Garat","doi":"10.1016/S0307-4412(99)00066-7","DOIUrl":"10.1016/S0307-4412(99)00066-7","url":null,"abstract":"<div><p>The electrophoretic mobility shift assay is a simple and rapid method for visualizing the existence of specific DNA-protein interactions and is a useful teaching experiment. In the experiment described here the students prepare nuclear extracts, a DNA probe, and can successfully produce specific DNA-protein complexes and estimate physicochemical parameters characterizing the interaction. The system described is easy to perform. The synthesis of the oligonucleotides required and the labeled deoxyribonucleotide used for labeling the probe are the main costs of the experiment, but since the synthesized oligonucleotides can be used in several courses and the amount of labeled deoxyribonucleotide required for labeling the probe is small the experiment is relatively inexpensive to put on with a class.</p></div>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"27 4","pages":"Pages 232-236"},"PeriodicalIF":0.0,"publicationDate":"1999-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(99)00066-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56622832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
A laboratory class experiment to detect and quantify lovastatin in commercial medicaments 商用药物中洛伐他汀的检测与定量实验
Biochemical education Pub Date : 1999-10-01 DOI: 10.1016/S0307-4412(99)00058-8
Walter Giordano, Carlos E. Domenech
{"title":"A laboratory class experiment to detect and quantify lovastatin in commercial medicaments","authors":"Walter Giordano,&nbsp;Carlos E. Domenech","doi":"10.1016/S0307-4412(99)00058-8","DOIUrl":"10.1016/S0307-4412(99)00058-8","url":null,"abstract":"<div><p>This article describes a laboratory class experiment for an undergraduate Biochemistry course for Chemistry students. The experimental work involves the extraction of lovastatin from a medicament utilized in the treatment of hypercholesterolemic patients, and its determination by spectroscopy and by reversed-phase high-performance liquid chromatography. The entire experiment can be performed in one laboratory period, e.g. less than one day.</p></div>","PeriodicalId":80258,"journal":{"name":"Biochemical education","volume":"27 4","pages":"Pages 229-231"},"PeriodicalIF":0.0,"publicationDate":"1999-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0307-4412(99)00058-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"56622947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
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