Methods in cell science : an official journal of the Society for In Vitro Biology最新文献_第5页

Membrane oxidative damage and apoptosis in cervical carcinoma cells of patients after radiation therapy. 宫颈癌放疗后细胞膜氧化损伤及细胞凋亡的研究。

Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2002-01-01

S M Bhosle, B N Pandey, N G Huilgol, K P Mishra

引用次数: 0

High resolution flow cytometric analysis of electronic nuclear volume and DNA content in normal and abnormal human tissue. 正常和异常人体组织中电子核体积和DNA含量的高分辨率流式细胞术分析。

Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2002-01-01 DOI: 10.1023/a:1024100705728

Richard A Thomas, Awtar Krishan, Michael Brochu

{"title":"High resolution flow cytometric analysis of electronic nuclear volume and DNA content in normal and abnormal human tissue.","authors":"Richard A Thomas, Awtar Krishan, Michael Brochu","doi":"10.1023/a:1024100705728","DOIUrl":"https://doi.org/10.1023/a:1024100705728","url":null,"abstract":"Background: The NPE Analyzer flow cytometer can simultaneously analyze the electronic nuclear volume (ENV) and DNA content of cells. This study describes the schematics, resolution, reproducibility, and sensitivity of biological standards analyzed on this unit.Methods: Calibrated beads and biological standards (lymphocytes, trout erythrocytes [TRBC], calf thymocytes, and tumor cells) were analyzed for ENV versus DNA content. Parallel data (forward scatter versus DNA) from a conventional flow cytometer were obtained.Results: ENV linearity studies yielded an R value of 0.999. TRBC had a coefficient of variation (CV) of 1.18 +/- 0.13. DNA indexes as low as 1.02 were detectable. DNA content of lymphocytes from 42 females was 1.9% greater than that for 60 males, with a noninstrumental variability in total DNA content of 0.5%. The ENV/DNA ratio was constant in 15 normal human tissue samples, but differed in the four animal species tested. The ENV/DNA ratio for a hypodiploid breast carcinoma was 2.3 times greater than that for normal breast tissue.Conclusions: The high-resolution ENV versus DNA analyses are highly reliable, sensitive, and can be used for the detection of near-diploid tumor cells that are difficult to identify with conventional cytometers. ENV/DNA ratio may be a useful parameter for detection of aneuploid populations.","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"24 1-3","pages":"11-8"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1024100705728","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22444290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Androgen receptor expression in archival human breast tumors. 人乳腺肿瘤档案中雄激素受体的表达。

Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2002-01-01 DOI: 10.1023/a:1024137729835

Poonam Arya, Ilia H Andritsch, Awtar Krishan

{"title":"Androgen receptor expression in archival human breast tumors.","authors":"Poonam Arya, Ilia H Andritsch, Awtar Krishan","doi":"10.1023/a:1024137729835","DOIUrl":"https://doi.org/10.1023/a:1024137729835","url":null,"abstract":"Hormone receptors play a major role in growth and hormonal therapy of breast and prostate tumors. Quantitative results from the ligand binding assays cannot determine heterogeneity in receptor expression nor can they discriminate between expression of the stromal and the tumor cells. Availability of antibodies to hormone receptors has led to the development of immunohistochemistry as a standard method for monitoring of hormone receptor expression under a microscope. However, this method is based on examination of a small number of cells. Laser flow cytometry has been extensively used for monitoring of receptor expression in human liquid tumors. As most of the hormone receptor expression is nuclear, we have developed methods for flow cytometric analysis of receptor expression in nuclei isolated from enzyme treated paraffin sections. The present report based on gated analysis of androgen receptor expression in nuclei isolated from archival formalin fixed/paraffin embedded breast tumors shows that receptor expression in aneuploid sub-populations is greater than that of the diploid cells.","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"24 1-3","pages":"61-4"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1024137729835","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22444204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Host-pathogen interactions: leukocyte phagocytosis and associated sequelae. 宿主-病原体相互作用:白细胞吞噬和相关后遗症。

Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2002-01-01 DOI: 10.1023/a:1024154200702

Jovanka M Voyich, Frank R DeLeo

{"title":"Host-pathogen interactions: leukocyte phagocytosis and associated sequelae.","authors":"Jovanka M Voyich, Frank R DeLeo","doi":"10.1023/a:1024154200702","DOIUrl":"https://doi.org/10.1023/a:1024154200702","url":null,"abstract":"Polymorphonuclear leukocytes (PMNs) are a critical component of the human innate immune response and are the first line of defense against invading microorganisms. Phagocytosis of invading microbes induces production of reactive oxygen species (ROS) by PMNs, which facilitates bactericidal activity. In addition to eliminating microorganisms, phagocytosis also accelerates PMN apoptosis, a process critical for resolution of inflammation. Inasmuch as leukocyte phagocytosis and ROS production are key components of the innate immune response, we developed flow cytometric methods to evaluate these processes in human PMNs. In contrast to traditional microscopy-based analyses, the methods described herein provide objective and high throughput measures of host cell-pathogen interactions. Importantly, they can be adapted for use with a number of fluorometric probes, and bacterium and host cell of choice, and each is based upon a common phagocytosis assay system. We also describe methods to measure phagocytosis-induced PMN apoptosis with this assay system. These methods entail detecting surface-exposed phosphatidylserine (early apoptosis), and measuring PMN chromatin condensation and DNA fragmentation (late apoptosis). Taken together, these assays provide rapid and accurate assessment of critical PMN processes.","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"24 1-3","pages":"79-90"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1024154200702","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22444712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 30

A simple flow-through micro-chamber for handling fragile, small tissue explants and single non-adherent cells. 一个简单的流动通过微室处理脆弱，小组织外植体和单个非贴壁细胞。

Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2002-01-01 DOI: 10.1023/a:1024445010789

Peter Hausen, Metta Riebesell

引用次数: 4

Androgen receptor expression in archival human breast tumors. 人乳腺肿瘤档案中雄激素受体的表达。

Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2002-01-01 DOI: 10.1007/978-94-017-0623-0_9

Poonam Arya, I. Andritsch, A. Krishan

引用次数: 0

Chromosome sorting and genomics. 染色体分选和基因组学。

Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2002-01-01 DOI: 10.1023/a:1024108923475

L Scott Cram, Carolyn S Bell, John J Fawcett

引用次数: 6

Flow cytometric monitoring of drug resistance in human tumor cells. 人肿瘤细胞耐药的流式细胞术监测。

Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2002-01-01 DOI: 10.1023/a:1024185612997

Awtar Krishan

引用次数: 5

Flowcytometric detection of PNH defect and response to therapy in aplastic anemia patients. 再生障碍性贫血患者PNH缺陷的流式细胞检测及对治疗的反应。

Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2002-01-01 DOI: 10.1023/a:1024150032561

Neelam Varma, Subhash Varma, Harpreet Vohra, Kiran Malik, Gurjeevan Garewal

引用次数: 0

Flow cytometric techniques for the detection of microorganisms. 微生物检测的流式细胞术技术。

Methods in cell science : an official journal of the Society for In Vitro Biology Pub Date : 2002-01-01 DOI: 10.1023/a:1024106317540

Hazel M Davey

{"title":"Flow cytometric techniques for the detection of microorganisms.","authors":"Hazel M Davey","doi":"10.1023/a:1024106317540","DOIUrl":"https://doi.org/10.1023/a:1024106317540","url":null,"abstract":"Flow cytometry (FCM) is a technique, which allows one to analyse cells rapidly and individually, and permits the quantitative analysis of distributions of a property or properties in a population. It therefore offers many advantages over conventional measurements for the analysis of biological cells. Historically the technique has been widely applied for the study of mammalian cells, but its use in microbiology has been more limited; this is mainly a consequence of the smaller size of microbes, which results in the smaller optical signals that can be obtained from them. Developments in light sources and optics, together with brighter, spectrally-diverse dyes have reduced this barrier over recent years and the flow cytometer is now an essential tool in many microbiological research establishments. FCM has an increasing role to play in the detection of microbes in both industrial and clinical settings. Environmental monitoring to prevent outbreaks of human diseases such as cryptosporidiosis and Legionnaires' disease and to detect acts of biowarfare or bioterrorism are all amenable to flow cytometric study. This review seeks to highlight the role of the flow cytometer in the detection of microbial cells.","PeriodicalId":80082,"journal":{"name":"Methods in cell science : an official journal of the Society for In Vitro Biology","volume":"24 1-3","pages":"91-7"},"PeriodicalIF":0.0,"publicationDate":"2002-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1023/a:1024106317540","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22444713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 59