Annals of Clinical Biochemistry最新文献_第5页

Stability of electrolytes in whole blood at room temperature prior to centrifugation: Implications for clinical analysis. 室温下离心前全血电解质的稳定性：临床分析的意义。

IF 1.1 4区医学

Annals of Clinical Biochemistry Pub Date : 2026-07-07 DOI: 10.1177/00045632261470646

Sayamon Janyapaisarn, Maneenooch Torngsawarng, Napaporn Inganuruksakul, Sumana Mas-Oodi, Theerawut Chanmee, Sureerut Porntadavity

{"title":"Stability of electrolytes in whole blood at room temperature prior to centrifugation: Implications for clinical analysis.","authors":"Sayamon Janyapaisarn, Maneenooch Torngsawarng, Napaporn Inganuruksakul, Sumana Mas-Oodi, Theerawut Chanmee, Sureerut Porntadavity","doi":"10.1177/00045632261470646","DOIUrl":"10.1177/00045632261470646","url":null,"abstract":"Background/ObjectivesThe pre-analytical stability of electrolytes in capped whole blood at room temperature remains uncertain due to limited studies and inconsistent findings. This study investigates electrolyte stability during delayed centrifugation, in accordance with recent EFLM recommendations.Materials and methodsThe stability of electrolyte concentrations (sodium, potassium, chloride, and bicarbonate) was assessed in capped whole blood samples from 40 participants stored at room temperature at three time intervals (0, 2, and 4 h) prior to centrifugation. Differences in electrolyte concentrations were expressed as percent deviation (%PD) from baseline (T0). The %PD for each analyte was calculated and compared with the maximum permissible difference (MPD), derived from intra- and inter-individual biological variation.ResultsThe calculated MPD values for sodium, potassium, chloride, and bicarbonate were 1.5%, 4.2%, 1.9%, 10.8%, respectively. The mean %PD at 2 h for sodium, potassium, chloride, and bicarbonate was 0.2, 0.1, -0.4, and -0.3, respectively. At 4 h, the mean %PD values were 0.3, 2.1, -0.7, and -0.4, respectively. All mean %PD values remained within the MPD limits at both 2 and 4 h, with potassium showing a higher mean %PD at the 4-h time point compared to 2 h.ConclusionsSodium and potassium concentrations increase over time, while chloride and bicarbonate concentrations decrease. Electrolytes in capped whole blood stored at room temperature with delayed centrifugation remained stable for up to 4 h. However, potassium appeared to be more susceptible to changes due to delayed processing. Timely sample handling is therefore critical to ensuring accurate electrolyte measurements.","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":" ","pages":"45632261470646"},"PeriodicalIF":1.1,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148395755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of Jaffe, Enzymatic, and Mass Spectrometric Methods for Creatinine Measurement in Protein-Positive and Hemoglobin-Positive Urine Samples. 蛋白质阳性和血红蛋白阳性尿样中肌酐测定的Jaffe、酶和质谱方法的比较。

IF 1.1 4区医学

Annals of Clinical Biochemistry Pub Date : 2026-07-07 DOI: 10.1177/00045632261470633

Gulsum Abuşoğlu, Mohammad Ahmad Bik, Firdevs Sak, Duygu Eryavuz Onmaz, Ali Unlu, Fatma Hümeyra Yerlikaya, Sedat Abuşoğlu, Yasemin Coşkun Yavuz

{"title":"Comparison of Jaffe, Enzymatic, and Mass Spectrometric Methods for Creatinine Measurement in Protein-Positive and Hemoglobin-Positive Urine Samples.","authors":"Gulsum Abuşoğlu, Mohammad Ahmad Bik, Firdevs Sak, Duygu Eryavuz Onmaz, Ali Unlu, Fatma Hümeyra Yerlikaya, Sedat Abuşoğlu, Yasemin Coşkun Yavuz","doi":"10.1177/00045632261470633","DOIUrl":"https://doi.org/10.1177/00045632261470633","url":null,"abstract":"Accurate measurement of urine creatinine is essential for evaluating renal function. Although the Jaffe and enzymatic methods are routinely applied in clinical laboratories, various urinary compounds can interfere with these assays. This study investigated the effects of hemoglobin and protein positivity on creatinine measurement and partially validated a mass spectrometric (LC-MS/MS) procedure. Urine samples from 136 participants were categorized based on dipstick results into three groups: negative (n = 55), hemoglobin-positive (n = 51), and protein-positive (n = 30). Creatinine concentrations were measured using Jaffe and enzymatic methods on an Architect c16000 analyzer and by LC-MS/MS on an API 3200 system. The LC-MS/MS method demonstrated linearity between 3-350 mg/dL, intra- and inter-assay coefficients of variation of 3.2-3.8% and 1.9-2.9%, and bias values ranging from 1.1% to 18.2%. Correlations among LC-MS/MS, enzymatic, and Jaffe methods ranged from 0.981 to 0.998. Bland-Altman analysis showed mean absolute biases of 0.06 to 17.33 mg/dL (1.48-19.42%) in controls, 1.44 to 4.78 mg/dL (0.79-9.59%) in hemoglobin-positive samples, and 2.18 to 9.87 mg/dL (0.52-14.39%) in protein-positive samples. Regression analysis showed slopes closer to unity in hemoglobin- and protein-positive samples compared to controls, likely reflecting higher creatinine concentrations in these groups. Despite this apparent improvement, bias values increased, particularly in protein-positive samples. Both routine methods showed acceptable agreement with LC-MS/MS, while the enzymatic method demonstrated slightly closer agreement under these conditions. LC-MS/MS maintained highest analytical specificity. These findings highlight the importance of method selection for reliable interpretation of renal function, especially in patients presenting with hematuria or proteinuria.","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":" ","pages":"45632261470633"},"PeriodicalIF":1.1,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148395616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Limitations of spectrophotometry in the analysis of cerebrospinal fluid bilirubin. 分光光度法分析脑脊液胆红素的局限性。

IF 1.1 4区医学

Annals of Clinical Biochemistry Pub Date : 2026-07-05 DOI: 10.1177/00045632261466866

Katherine Birch, Maria Squires, Miles Chapman, Melanie Hart, Carrie Chadwick, John Goodfellow, Ravishankar Sargur, Bushra Zaidi, James Hoy, Michael Lunn, Dina Patel

{"title":"Limitations of spectrophotometry in the analysis of cerebrospinal fluid bilirubin.","authors":"Katherine Birch, Maria Squires, Miles Chapman, Melanie Hart, Carrie Chadwick, John Goodfellow, Ravishankar Sargur, Bushra Zaidi, James Hoy, Michael Lunn, Dina Patel","doi":"10.1177/00045632261466866","DOIUrl":"https://doi.org/10.1177/00045632261466866","url":null,"abstract":"BackgroundCerebrospinal fluid (CSF) spectrophotometry is well-established in the UK for measuring CSF bilirubin and oxyhaemoglobin to aid in the diagnosis of subarachnoid haemorrhage. Although it is known that some antibiotics and large amounts of oxyhaemoglobin interfere in the spectrophotometric analysis of CSF bilirubin, there is little published evidence about the impact of such interferences.MethodsExperimental samples were distributed to participants in the UK National External Quality Assessment Service (NEQAS) for Immunology, Immunochemistry and Allergy EQA programme for CSF haem pigments. Distribution 244 consisted of a pair of matched samples but with sample 244-2 containing an increased amount of oxyhaemoglobin compared to sample 244-1. Distribution 251 consisted of a pair of matched samples but with sample 251-2 containing additional doxycycline at a concentration of 0.5 µg/mL compared to sample 251-1. Participants analysed the samples spectrophotometrically and absorbance values were returned to UK NEQAS.ResultsThe net bilirubin absorbance (NBA) was significantly reduced in the presence of both interferants; adding 0.6% oxyhaemoglobin decreased the NBA by a mean of 55.9% and adding 0.5 µg/mL doxycycline decreased the NBA by a mean of 14.3%. Wilcoxon signed rank tests showed the NBA was significantly different for sample 2 compared to sample 1 for both distributions.ConclusionsIncreased amounts of oxyhaemoglobin and the presence of doxycycline can both negatively interfere with NBA. The interference is subtle and difficult to detect but has the potential to change a true positive result to a false negative, highlighting a significant limitation of the CSF spectrophotometry technique.","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":" ","pages":"45632261466866"},"PeriodicalIF":1.1,"publicationDate":"2026-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148389901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Commentary on the impact of lithium heparin vacutainer tubes on daily cerebrospinal fluid analysis. 肝素锂真空管对日常脑脊液分析的影响。

IF 1.1 4区医学

Annals of Clinical Biochemistry Pub Date : 2026-07-05 DOI: 10.1177/00045632261466862

Valery Brunel

引用次数: 0

Estimation of half-life with confidence interval for phosphatidylethanol (PEth) in blood based on two consecutive measurements. 基于两次连续测量的血液中磷脂酰乙醇（PEth）半衰期的置信区间估计。

IF 1.1 4区医学

Annals of Clinical Biochemistry Pub Date : 2026-07-01 Epub Date: 2025-12-15 DOI: 10.1177/00045632251411787

Johan Bjerner, Michael Sovershaev, Elisabeth Wiik Vigerust, Thor Hilberg, Johannes Østrem Fjøse, Tom Rune Karlsen

{"title":"Estimation of half-life with confidence interval for phosphatidylethanol (PEth) in blood based on two consecutive measurements.","authors":"Johan Bjerner, Michael Sovershaev, Elisabeth Wiik Vigerust, Thor Hilberg, Johannes Østrem Fjøse, Tom Rune Karlsen","doi":"10.1177/00045632251411787","DOIUrl":"10.1177/00045632251411787","url":null,"abstract":"BackgroundPhosphatidylethanol (PEth) is formed in erythrocyte membranes after alcohol consumption. When abstaining, the PEth level falls with a rate proportional to its concentration, and a short apparent PEth half-life supports abstinence. We here derive algorithms for calculating unbiased half-lives and confidence intervals (CIs).MethodsPEth was measured using Acquity UPC2-MS/MS systems in clinical blood samples from out-patients. We identified 6989 individuals having taken two or more PEth samples within 28 days. One measurement pair was randomly selected from everyone. We derived methods for and calculated PEth half-lives and corresponding 95% CIs for exact, rounded, and truncated data, on closed form, Monte Carlo methods and No-U-turn sampling.ResultsThe peak of the PEth half-life was at 8.62 days. Peak PEth half-life was 8.72 days for men and 8.47 days for women (P = 0.028) and on age: 8.55 days for age 18-39 years, 8.56 for age 40-59 years and 8.87 for 60+ years (P = 0.026). PEth concentration did not significantly affect half-life. CIs on a closed form performed excellently on exact data, with misclassification of abstinence for 16 out of 6989 observations (0.23%). When rounding or truncating data, misclassification occurred using Monte Carlo methods in 104 (1.5%) and 127 (1.8%) of the observations and using closed form algorithms in 855 (12.2%) and 777 (11.1%).ConclusionUnbiased PEth half-lives and CIs can be calculated and put into use in laboratory information systems. Rounding or truncating data used for PEth half-life calculation widened CIs with misinterpretations of alcohol abstinence.","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":" ","pages":"319-326"},"PeriodicalIF":1.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145761892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing laboratory quality through comprehensive risk management and process analysis. 通过全面的风险管理和过程分析提高实验室质量。

IF 1.1 4区医学

Annals of Clinical Biochemistry Pub Date : 2026-07-01 Epub Date: 2026-02-02 DOI: 10.1177/00045632261424771

Shobha C Ramachandra, Venkatesha Madegowda, Manish Kumar Pandey, Akila Prashant, Kusuma K Shivashankar, Swetha N Kempegowda

{"title":"Enhancing laboratory quality through comprehensive risk management and process analysis.","authors":"Shobha C Ramachandra, Venkatesha Madegowda, Manish Kumar Pandey, Akila Prashant, Kusuma K Shivashankar, Swetha N Kempegowda","doi":"10.1177/00045632261424771","DOIUrl":"10.1177/00045632261424771","url":null,"abstract":"BackgroundLaboratory test results are crucial for clinical decisions, but errors at any stage can compromise patient safety. Despite technological advances and quality systems, laboratory processes remain vulnerable, necessitating structured risk assessment. Limited research exists on integrating Failure Mode and Effects Analysis (FMEA) with Sigma metrics. This study addresses these gaps through a comprehensive risk assessment in a NABL-accredited tertiary care hospital laboratory.MethodsA retrospective observational study was conducted over 2.5 years. FMEA was used to identify errors across all phases of the total testing process. Quarterly error rates were calculated, converted to defects per million (DPM), and expressed as Sigma metrics. Risk Priority Numbers (RPN = severity × detectability × frequency) were scored quarterly to prioritize failures. The effect of corrective actions was evaluated by comparing pre- and post-intervention Sigma values.ResultsAcross 10 quarters (five pre- and five post-intervention), 23 error types were identified. The highest RPNs (>20) were observed for haemolyzed samples, clotted samples, and non-intimation of critical values. Haemolyzed samples declined from 0.97% to 0.49% (ARR 0.48%; RR 0.51; 95% CI 0.47-0.55; P < .0001). Clotted samples reduced from 0.24% to 0.06% (ARR 0.18%; RR 0.24; 95% CI 0.20-0.29; P < .0001). Non-intimation of critical values decreased from 2.63% to 2.18% (RR 0.83; 95% CI 0.70-0.99; P = .065).ConclusionIntegrating FMEA with Sigma metrics provides a robust framework for identifying, prioritizing, and reducing laboratory errors. Continuous monitoring and corrective action are essential to sustain improvements in laboratory quality and patient safety.","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":" ","pages":"345-356"},"PeriodicalIF":1.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146103593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Routine laboratory triage for NT-proBNP elevation may reflect cardiorenal frailty as much as heart failure specificity. NT-proBNP升高的常规实验室分诊可能反映心肾虚弱和心力衰竭的特异性。

IF 1.1 4区医学

Annals of Clinical Biochemistry Pub Date : 2026-07-01 DOI: 10.1177/00045632261466859

Zhanyun Liu

引用次数: 0

Elevated haemoglobin A1c confounds thalassaemia diagnosis by lowering HbA2 levels. 升高的A1c通过降低HbA2水平混淆了地中海贫血的诊断。

IF 1.1 4区医学

Annals of Clinical Biochemistry Pub Date : 2026-07-01 Epub Date: 2025-10-27 DOI: 10.1177/00045632251395562

Jeroen Slaats, Erfan Nur, Harriët Heijboer, Eva Rettenbacher, Rob van Zwieten, Esther Miltenburg, Joyce Reijerse, Nienke Verhaart, Wouter van den Eshof, Bart J Biemond, Henrike M Hamer

{"title":"Elevated haemoglobin A1c confounds thalassaemia diagnosis by lowering HbA2 levels.","authors":"Jeroen Slaats, Erfan Nur, Harriët Heijboer, Eva Rettenbacher, Rob van Zwieten, Esther Miltenburg, Joyce Reijerse, Nienke Verhaart, Wouter van den Eshof, Bart J Biemond, Henrike M Hamer","doi":"10.1177/00045632251395562","DOIUrl":"10.1177/00045632251395562","url":null,"abstract":"Background: Thalassemias are inherited disorders caused by reduced production of structurally normal haemoglobin chains. Haemoglobin A2 (HbA2) constitutes an important parameter in the diagnostic evaluation of thalassaemias. Insight into the factors that modulate HbA2 levels is critical for correct interpretation of laboratory results in cases where thalassaemia is suspected. Methods: A retrospective study was conducted on patients who underwent haemoglobin analysis by high-performance liquid chromatography (HPLC) at Amsterdam UMC. Patients with elevated haemoglobin A1c (HbA1c) levels due to chronic hyperglycaemia were compared with controls, an iron deficiency cohort, and α-thalassemia cohorts. Results: Patients with strongly elevated HbA1c levels (110-180 mmol/mol) showed significantly reduced HbA2 levels compared with controls (2.0% vs. 2.4%, P<0.0001). This reduction in HbA2 fraction was not observed when HbA2 was expressed relative to non-glycated HbA instead of all haemoglobin fractions. Red cell indices (MCH, MCV) and haemoglobin concentrations remained unaffected. The degree of HbA2 reduction in patients with high HbA1c was comparable to that observed in iron deficiency and α-thalassemia. Conclusions: Elevated HbA1c levels due to chronic hyperglycaemia lower measured HbA2 fractions, confounding the diagnostic evaluation of thalassaemias. Laboratories should consider HbA1c status when interpreting HbA2 results in patients with poorly controlled diabetes. Expressing HbA2 relative to non-glycated HbA may improve diagnostic accuracy in such cases.","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":" ","pages":"357-361"},"PeriodicalIF":1.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145375900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improving the PLASMIC score with the use of RDW-SD in the differential diagnosis for thrombotic thrombocytopenic purpura in Chinese patients. 应用RDW-SD提高血浆评分在中国血栓性血小板减少性紫癜患者鉴别诊断中的价值

IF 1.1 4区医学

Annals of Clinical Biochemistry Pub Date : 2026-07-01 Epub Date: 2025-12-15 DOI: 10.1177/00045632251411788

Chi Zhang, Libo Xia, Xuesong Lv, Weixun Sun, Zhigang Xiong, Yang Fei

{"title":"Improving the PLASMIC score with the use of RDW-SD in the differential diagnosis for thrombotic thrombocytopenic purpura in Chinese patients.","authors":"Chi Zhang, Libo Xia, Xuesong Lv, Weixun Sun, Zhigang Xiong, Yang Fei","doi":"10.1177/00045632251411788","DOIUrl":"10.1177/00045632251411788","url":null,"abstract":"BackgroundThis study aimed to identify laboratory parameters that could optimize the PLASMIC score, thereby improving its diagnostic accuracy for thrombotic thrombocytopenic purpura (TTP).MethodsWe performed a retrospective analysis of 136 patients with suspected TTP who had available ADAMTS-13 activity measurements. Patients were stratified into two groups based on ADAMTS-13 activity: a TTP group (n = 49) and a non-TTP group (n = 87). Routine laboratory parameters-including hemoglobin (HGB), red blood cell distribution width-standard deviation (RDW-SD), mean corpuscular volume (MCV), platelet count (PLT), alanine aminotransferase (ALT), aspartate aminotransferase (AST), direct bilirubin (DBIL), indirect bilirubin (IBIL), lactate dehydrogenase (LDH), creatinine (CR), urea (UREA), international normalized ratio (INR), and D-dimer-were compared between groups. Statistically significant parameters were selected as candidate variables to refine the PLASMIC score. The diagnostic performance of the modified model was evaluated using receiver operating characteristic (ROC) curve analysis.ResultsSix parameters-HGB, PLT, ALT, RDW-SD, IBIL, and LDH-demonstrated significant differences between TTP and non-TTP patients. Multivariate logistic regression identified RDW-SD, PLT, and LDH as independent predictors of TTP. Based on these findings, we revised the PLASMIC score by substituting MCV with RDW-SD. The modified model exhibited a higher area under the curve (AUC) (0.907 vs 0.817) while maintaining sensitivity (95.9%) and improving specificity (70.1% vs 65.9%) compared to the original.ConclusionThe modified PLASMIC score model may improve diagnostic accuracy for TTP in similar patient populations, but requires external validation in diverse cohorts to confirm its broader utility.","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":" ","pages":"327-335"},"PeriodicalIF":1.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145761959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A UK & Ireland national audit of urine acidification. 英国和爱尔兰尿液酸化的国家审计。

IF 1.1 4区医学

Annals of Clinical Biochemistry Pub Date : 2026-07-01 Epub Date: 2025-12-15 DOI: 10.1177/00045632251411791

Emma Hughes, Louise Ward, Wassif S Wassif

{"title":"A UK & Ireland national audit of urine acidification.","authors":"Emma Hughes, Louise Ward, Wassif S Wassif","doi":"10.1177/00045632251411791","DOIUrl":"10.1177/00045632251411791","url":null,"abstract":"ObjectivesAcidification of urine samples has long been used as a method of preservation to enhance analyte stability. However, there are inherent safety risks to staff and patients when acid preservatives are used. The Association of Laboratory Medicine National Audit Committee sought to assess urine acidification practices in NHS laboratories.MethodAn 11 question survey was sent to all Association of Laboratory Medicine members for completion between 24th January 2023 and 24th February 2023 and data analysis performed using Microsoft Excel. For a variety of analytes, laboratories were asked to detail the type of recommended and accepted collection containers, whether 24 h and/or spot urine samples were accepted, if preservative was added to samples on receipt if not collected with preservative and the storage conditions for unpreserved samples.Results69 laboratories responded. Safety information was provided to users by the majority of laboratories and 88% of laboratories would pH test samples prior to sending them to a referral laboratory if acidification was a prerequisite. Variation was noted in quoted time of sample stability when refrigerated. Laboratories provided answers about specific tests - sodium, potassium, osmolality, calcium, magnesium, phosphate, creatinine, Bence Jones protein, total protein, urate, citrate, oxalate, cysteine, catecholamines, metanephrines, 5-HIAA, VMA/HMMA, copper, amino acids, organics acids and glycosaminoglycans.ConclusionsThere is significant variation in the use of acid as a preservative for urine samples throughout NHS laboratories as well as historical requirements for urine acidification for certain analytes which evidence has indicated is no longer a requirement.","PeriodicalId":8005,"journal":{"name":"Annals of Clinical Biochemistry","volume":" ","pages":"336-344"},"PeriodicalIF":1.1,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145761915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0