Abdulla L Jiad, May K Ismael, Taher A Salih, Salma N Malik, Bahaa Abdullah Laftaah Al-Rubaii
{"title":"Genotyping and evaluation of interleukin-10 and soluble HLA-G in abortion due to toxoplasmosis and HSV-2 infections.","authors":"Abdulla L Jiad, May K Ismael, Taher A Salih, Salma N Malik, Bahaa Abdullah Laftaah Al-Rubaii","doi":"10.17420/ap6802.444","DOIUrl":"https://doi.org/10.17420/ap6802.444","url":null,"abstract":"<p><p>Abortion is a condition that occurs due to one of the pathological injuries, often one of the members of the TORCH is the real cause. The current study aimed to investigate the impact of toxoplasmosis, HSV-2 infections with abortion, and also, the identification of immunogenetics marker (interleukin-10) that may be associated with abortion. Anti-Toxoplasma IgG, IgM, Herpes simplex virus-2 IgM, human soluble leukocyte antigen class I-G and interleukin-10 were estimated by ELISA technique, while the expression of IL-10 gene was investigated by using the real-time PCR. The results showed that among aborted women the rate of anti-Toxoplasma and HSV-2 IgM antibodies occurred within the age groups (21-30) years and (31-40) years 32(100.0%) and 36(100.0% ), respectively. A significant relationship was found between IL-10 and cases with a P=0.005. The pattern of distribution of HLA-G in the studied groups showed that there was a significant relationship between HLA-G and cases with a P=0.005. Regarding the IL-10 rs gene, the results revealed an amplified product of 377 bp and there was a high Ct value for patients and controls with a high Ct value of templates, preoperational to the gene concentration. We concluded that there was a significant relationship between human leukocyte antigen-G and the cases. It was found that there was a high Ct value for patients and controls with a high Ct value for templates.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":" ","pages":"385-390"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40488392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mounir Abi-Said, Jessica Bou Shroush, Sarah Karam, Houssam Shaib
{"title":"Intestinal parasites of Apodemus mystacinus along altitudinal stratification of Ibrahim River, Mount Lebanon.","authors":"Mounir Abi-Said, Jessica Bou Shroush, Sarah Karam, Houssam Shaib","doi":"10.17420/ap6802.428","DOIUrl":"https://doi.org/10.17420/ap6802.428","url":null,"abstract":"<p><p>This study documents the diversity of intestinal parasites in the eastern broad-toothed field mouse (Apodemus mystacinus) along the altitudinal gradient of Ibrahim River in Mount Lebanon during the spring and autumn of 2017. A total of 190 A. mystacinus were trapped in seventeen sites (6 riparian, 11 non-riparian) and examined for intestinal parasites. Eight intestinal parasites were identified including Heligmosomoides polygyrus, Trichuris muris, Syphacia frederici, Protospirura muris, and Aspiculuris tetraptera (nematodes), Hymenolepis diminuta (cestode), Brachylaima spp. (trematode), and Eimeria alorani (coccidian). Most of the trapped mice (85%) were infected with intestinal parasites and 38.84% showed concurrent infection with at least two parasitic species. The season had a significant effect on mice infection with Syphacia frederici, and mice are most likely to have infection with this nematode in spring season. The vegetation zone had a significant overall impact on mice infection with the nematodes Heligmosomoides polygyrus and Trichuris muris while gender did not influence significantly mouse infection with intestinal parasites. This is the first study that has been carried out to identify the intestinal parasite community in A. mystacinus of Ibrahim River region. The findings pave the way for future studies on intestinal parasites in rodents and the environmental variations affecting their dynamics.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":" ","pages":"227-239"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40504476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"In vivo comparative study of the efficacy of β-sitosterol, ketoconazole 2% and mupirocin for the treatment cutaneous leishmaniosis.","authors":"Alyaa A Hafedh","doi":"10.17420/ap6802.431","DOIUrl":"https://doi.org/10.17420/ap6802.431","url":null,"abstract":"<p><p>Cutaneous leishmaniosis (CL) is an important parasitic disease characterized by specific skin lesion, includes the vector that cause the CL and treatment in general. The study aimed to identify the effect of three different drugs which are β-sitosterol, ketoconazole 2% and mupirocin in the treatment of cutaneous leishmaniosis. The study was conducted at Dermatological Unit of Al Hussein Teaching Hospital/Thi-Qar/Iraq from October to November 2021. The patients presented with a lesion will be involved in this study and its involved isolation of parasites from the lesion of patients and the parasite was replicated in (NNN) media then the inoculum was concentered. A total of 40 male of mice (Mus musculus) of BALB/c strain injected by parasite suspension, after the appearance of lesion, β-sitosterol, ketoconazole 2% and mupirocin was applied on a lesions daily for 2 weeks, the statistical analysis was done by SPSS program. In the current study, the β-sitosterol was most effective in the treatment of skin leishmanial lesion than the other drugs with mean is (11.9±1.449 mm) in compared with the other drugs under P-value < 0.046 with the complete recovery. β-sitosterol was highly effect on the L. tropica infections with complete recovery and no scar appearance than ketoconazole and mupirocin and can be used for treatment of the disease lesion.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":" ","pages":"257-261"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40504486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Mehdi Najm, Mahdi Fakhar, Mohammad Shokrzadeh Lamuki, Bahman Rahimi-Esboei, Farshideh Habibi, Mitra Sharbatkhori
{"title":"Corynosoma caspicum (Acanthocephala, Polymorphidae), as a heavy metal bioindicator in the fish Gasterosteus aculeatus from the Caspian Sea, northern Iran.","authors":"Mehdi Najm, Mahdi Fakhar, Mohammad Shokrzadeh Lamuki, Bahman Rahimi-Esboei, Farshideh Habibi, Mitra Sharbatkhori","doi":"10.17420/ap6803.460","DOIUrl":"https://doi.org/10.17420/ap6803.460","url":null,"abstract":"<p><p>Previous marine biology studies found that the concentration of heavy metals in some parasites of fish such as acanthocephalans can be a proper bioindicator. Therefore, we attempted to measure five heavy metal concentrations in the tissues of the fish Gasterosteus aculeatus (G. aculeatus) and its acanthocephalan parasites, Corynosoma caspicum (C. caspicum) from the Southern Caspian Sea, northern Iran. G. aculeatus (three-spined stickleback) was collected from the south of the Caspian Sea, Mazandaran Province, northern Iran. After tissue preparation, the heavy metal concentrations in fishes and acanthocephalans were obtained using the tissue dissolution technique and an atomic absorption spectrophotometer. The concentrations of chromium (Cr), cadmium (Cd), lead (Pb), zinc (Zn), and copper (Cu) in the skin, liver, muscle, and intestine tissues of the fish and its parasites, C. caspicum, were measured and compared. Eighty (32%) of 250 collected fish were infected by at least one acanthocephalan parasite. The Cr indicated the highest concentration (5.329±3.275) of the heavy metals in acanthocephalan, even more than the skin, liver, and muscle of infected fishes. Cd had the lowest concentration (0.0333±0.0075) of heavy metals in acanthocephalan, but it was still higher than the concentration in the infected fishes' skin, liver, muscle, and intestine tissues. Our findings indicated that C. caspicum parasites can be considered extremely sensitive early-alert bioindicators, particularly in sensitive and under-threat environments with low pollution levels.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"68 3","pages":"537-542"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10816198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Identification and phylogenic analysis of Leishmania species among patients and reservoir hosts based on N-acetylglucosamine-1-phosphate transferase gene in Central Iran.","authors":"Mojgan Farrokhi-Karibozorg, Homa Hajjaran, Zahra Ghayour-Najafabadi, Seyed Hossein Hejazi, Anjila Ataei-Pirkooh, Mehdi Mohebal","doi":"10.17420/ap6803.452","DOIUrl":"https://doi.org/10.17420/ap6803.452","url":null,"abstract":"<p><p>Leishmaniosis, a vector-born disease that infects humans and other vertebrates, is the result of infection with Leishmania species belong to the family Trypanosomatidae. The present study was performed to determine the status of cutaneous leishmaniosis in Isfahan province. Samples were taken from the margin of skin ulcers of patients with suspected CL referred to the medical health centers in Isfahan province. Also, ear and snout samples were taken from the rodents. In total, 85 parasitologically positive samples were subjected to the PCR-RFLP method based on the nagt gene for identification of Leishmania species, also 11 samples were subjected to sequencing and phylogenetic analysis. For all positive samples, a 1450-1460 bp band of the nagt gene was amplified in PCR method. The digestion pattern of ACC1 enzyme in 79 of patients indicated L. major and in one sample was similar to L. tropica. Four rodent reservoirs distingue as L. major and one sample as L. turanica. Phylogenetic analysis confirmed the species identification and three haplotypes were reported. The results of the current study showed that L. major is the predominant species of Leishmania parasites in Isfahan province and the main reservoir of CL is Rhombomys opimus. Also, the nagt gene is a useful and practical marker for determining different species of Leishmania parasites as well as their phylogenetic analysis.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"68 3","pages":"461-471"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10439064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Cynthia J González-Rivas, Gabriel N Castillo, Iván D Simoncelli
{"title":"Synhimantus (Dispharynx) nasuta (Nematoda: Acuariidae) on the Green-barred Woodpecker Colaptes melanochloros (Aves: Picidae) in Argentina.","authors":"Cynthia J González-Rivas, Gabriel N Castillo, Iván D Simoncelli","doi":"10.17420/ap6804.493","DOIUrl":"https://doi.org/10.17420/ap6804.493","url":null,"abstract":"<p><p>Only a very small percentage of the total bird species reported for Argentina has been studied from the parasitic point of view. This places wild birds as the least studied compared to other vertebrates. In light of this, the goal of the present study is to contribute to the knowledge of endoparasites on wild birds of Argentina. We analyzed a specimen of Green-barred Woodpecker, Colaptes melanochloros, which arrived in bad conditions at the Center for Wildlife Rehabilitation, Environmental Education and Responsible Recreation (Faunistic). The bird subsequently died and we proceeded to do a routine postmortem analysis. The analysis determined the presence of four nematodes (3 females and 1 male) of Synhimantus (Dispharynx) nasuta, this being the first record for the Green-barred Woodpecker Colaptes melanochloros in Argentina. This study provides novel information about parasitism in Argentina's birds.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"68 4","pages":"843-847"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141974922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"An updated insight into the gastrointestinal helminthoses of poultry: a review.","authors":"Wafaa A Abd El-Ghany","doi":"10.17420/ap6804.471","DOIUrl":"https://doi.org/10.17420/ap6804.471","url":null,"abstract":"<p><p>This review article provides more information about the incidence of helminths affect the gastrointestinal tracts of poultry in different countries, life cycle, clinical picture, diagnosis, and prevention and control measures of such infections. Backyard and deep litter production systems show higher helminth infections than cage system. Moreover, the incidence of helminth infection is more common in tropical countries of Africa and Asia than of European ones due to the suitability of environment and management conditions. Nematodes and cestodes are the most common gastrointestinal helminths of avian species, followed by trematodes. The life cycles of helminths may be direct or indirect, but the infection is usually through faecal-oral route. Affected birds show general signs, low production performance parameters, and even death due to intestinal obstruction and rupture. Lesions of the infected birds reveal catarrhal to haemorrhagic enteritis according to the severity of infection. Diagnosis of affection is mainly based on post mortem examination or microscopic detection of eggs or parasites. As internal parasites adversely affect the host causing poor feed utilization and low performance, thus intervention control strategies are urgent. Prevention and control strategies are relied on application of strict biosecurity measures, eradication of intermediate hosts, early routine diagnosis, and continuous application of specific anthelmintic drugs. Deworming using herbal medicine is recent and successful and may be good alternative to chemicals. In conclusion, helminth infections of poultry remain a major hurdle against the profitable production in poultry producing countries and necessary preventive and control measures should be strictly applied by poultry producers.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"68 4","pages":"645-656"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10757849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"First molecular evidence of Theileria lestoquardi in small ruminants in northern Iran.","authors":"Seyedmousa Motavallihaghi, Mahdi Fakhar, Mitra Sharbatkhori, Abdol Sattar Paghe","doi":"10.17420/ap6803.459","DOIUrl":"https://doi.org/10.17420/ap6803.459","url":null,"abstract":"<p><p>Ovine theileriosis as a critical agent in small ruminant production, can cause lethal infections. Different species of Theileria have been reported in various parts of the world, and each species causes different diseases in the host. This is the first molecular study to investigate the prevalence of ovine theileriosis and identify the dominant Theileria species in northern Iran. A number of 220 small ruminants, including sheep and goats, were randomly sampled from 22 flocks. Peripheral blood smears were stained by the Giemsa staining method. As well as for species identification, all samples were examined by PCR. From 220 samples, 160 and 60 were sheep and goat, respectively. By the Giemsa staining method, Theileria parasite was observed in 20 (9%) samples. But by polymerase chain reaction (PCR) method, 30 (13.6%) samples were positive for Theileria species. Theileria lestoquardi was the most common species found in these animals. The high prevalence of theileriosis in small ruminants demonstrates the emergence of ovine theileriosis in Mazandaran and Golestan provinces in northern Iran.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"68 3","pages":"531-536"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10815389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The effect of toxoplasmosis on renal function in hemodialysis patients.","authors":"Mustafa Ahmed Abood, Entsar Jabbar Saheb","doi":"10.17420/ap6804.475","DOIUrl":"https://doi.org/10.17420/ap6804.475","url":null,"abstract":"<p><p>Toxoplasma gondii is an obligate intracellular protozoan parasite; it spreads via the circulatory system during infection and causes chronic infection in various organs. Toxoplasmosis affects nearly one third of people worldwide, especially immunocompromised people. This study aimed to determine the effect of toxoplasmosis on renal function in hemodialysis patients. Overall 300 patients referred to the Medical City, Al-Karama General Hospital, Baghdad, Iraq were enrolled from 2021 to 2022. All serum samples were tested for T. gondii immunoglobulins (IgG and IgM) antibodies, urea and creatinine levels. In patients undergoing hemodialysis, the results revealed a high positivity percentage for anti-Toxoplasma IgG. In hemodialysis patients infected with T. gondii, the urea and creatinine levels were higher than the controls. The mean urea level was high in hemodialysis patients infected with toxoplasmosis compare with hemodialysis patient without toxoplasmosis in different gender and age while the level of creatinine had no significant differences in hemodialysis patient with or without toxoplasmosis. These finding suggest that the incidental rate of toxoplasmosis could be considered as an indication to the high risk of hemodialysis patients.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"68 4","pages":"685-692"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9989907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Past, present and future of Trichomonas vaginalis: a review study.","authors":"Rasha Khalil Abduljalil Alsaad","doi":"10.17420/ap6803.447","DOIUrl":"https://doi.org/10.17420/ap6803.447","url":null,"abstract":"<p><p>Trichomonas vaginalis (TV) is the most common non-viral sexually transmitted infection (STI) microaerophilic protist parasite, which is the causative agent of trichomonosis. Globally, the estimated annual incidence is more than 270 million cases. It is correlated for several health problems including pelvic inflammatory disease (PID), pregnancy miscarriages, cervical carcinoma, prostatitis, prostatic adenocarcinomas, infertility, and the acquisition of human immunodeficiency virus (HIV). Most individuals infected with TV are asymptomatic. Metronidazole (MTZ) has been the treatment of choice for women. Currently, there is no effective vaccine against this pathogen despite efforts at vaccine development. Different socio-economic, demographic, behavioral, and biological factors are associated with the disease. Apart from its role as a pathogenic agent of diseases, it is also a fascinating organism with a surprisingly large genome for a parasite, i.e. larger than 160 Mb, and physiology adapted to its microaerophilic lifestyle. Particularly, the hydrogenosome, a mitochondria-derived organelle that releases hydrogen, attracted much interest in the last decades and rendered TV a model organism for eukaryotic evolution. According to the high prevalence and health consequences associated with TV, there is a requirement for improved screening programs in Iraq. The early diagnosis of asymptomatic diseases and effective treatment regimens are mandatory. Despite being highly prevalent of trichomonosis in the world, there is no review research published that solely focuses on T. vaginalis infections in Iraq.</p>","PeriodicalId":7987,"journal":{"name":"Annals of parasitology","volume":"68 3","pages":"409-419"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10705355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}