Applied Ecology and Environmental Research最新文献

{"title":"CONTACT TOXICITY AND ANTIFEEDANT ACTIVITIES OF RHODODENDRON YUNNANENSE EXTRACTS AGAINST STORAGE DISEASE AND PESTS OF TRADITIONAL CHINESE MEDICINES","authors":"X.X. WU, G.F. LI, Y.R. HE, H.Q. ZHU, Y.Q. GAO, W. G. Su","doi":"10.15666/aeer/2403_42794297","DOIUrl":"https://doi.org/10.15666/aeer/2403_42794297","url":null,"abstract":"This study evaluated the contact-killing and anti-feeding activities of Yunnan Rhododendron (Rhododendron yunnanense) extracts and their isolated compounds against pests threatening stored traditional Chinese medicinal herbs. Extracts were prepared using methanol, 99% ethanol, petroleum ether, and deesterified methanol, followed by liquid-liquid extraction and chromatographic purification. Bioassays targeted Rhyzopertha dominica, Stegobium paniceum, and Ditylenchus dipsaci, with toxicity assessed via topical application and Tween 80 suspension methods, while anti-feeding activity was tested using flour-based assays. Key results showed that de-esterified methanol leaf extracts yielded the highest paste (31.25%), followed by 99% ethanol leaf extracts (30.58%). The 99% ethanol leaf extract exhibited the strongest contact-killing activity, with LD50 values of 15.76 μg/adult (R. dominica), 14.69 μg/adult (S. paniceum), and 0.74 mg/mL (D. dipsaci). Among isolated compounds, 4-methoxy-7-hydroxycoumarin demonstrated remarkable anti-feeding effects (75.24% refusal rate at 2.5 mg/mL), while apocynin showed potent contact toxicity (LD50 = 6.53 μg/adult for R. dominica). These findings highlight Yunnan Rhododendron. (R. yunnanense) as a promising source of natural insecticides for eco-friendly pest management in herbal storage. Further research should explore mechanistic pathways and field applicability.","PeriodicalId":7975,"journal":{"name":"Applied Ecology and Environmental Research","volume":"24 3","pages":"4279-4297"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://doi.org/10.15666/aeer/2403_42794297","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148305556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ROLE AND MECHANISM OF GMHORSTS GENE ON DROUGHT STRESS TOLERANCE OF SOYBEAN","authors":"Zhikang Li","doi":"10.15666/aeer/2302_26032619","DOIUrl":"https://doi.org/10.15666/aeer/2302_26032619","url":null,"abstract":"With the frequent occurrence of global drought, some countries and regions are facing reduced soybean production.Therefore, in order to increase soybean yield and ensure global food security, the influence and mechanism of soybean GmHORSTs gene on its drought stress tolerance are analyzed, in order to provide support for improving soybean drought resistance.The experimental results indicated that after drought stress, the fresh weight, dry weight, and water content of pTRV-GmHORSTs were 0.90 g, 0.48 g, and 36.7%,respectively, significantly lower than that of pTRV-00.The content of malondialdehyde and H2O2 were both higher than in pTRV-00, with values of 0.41 umlo/g FW and 13.69 umlo/g, respectively.The fresh weight, dry weight, and water content of GmHORSTs overexpressed plants after drought stress were 1.51 g, 0.59 g, and 61.2%, respectively, all higher than those of wild plants, while their H2O2 and propylene glycol contents were lower than those of wild plants.Under drought stress, the withering degree of pTRV-GmHORSTs was higher than that of normal plants, while the withering degree of over-expressed plants was significantly lower than that of wild plants.In addition, under drought stress conditions, the stomatal conductance of pTRV-GmHORSTs was 19.7 um 2 , which was higher than that of pTRV-00.The stomatal conductance of over-expressed plants was 3.9 um 2 , which was lower than that of wild plants.The abscisic acid content of pTRV-GmHORSTs was 45.3 ng/mL, which was lower than that of pTRV-00.The abscisic acid content of over-expressed plants was 84.8 ng/mL and 84.7 ng/mL, both higher than that of wild plants.The above results indicate that the GmHORSTs gene can increase stomatal closure by increasing leaf abscisic acid content, thereby reducing transpiration and improving soybean drought tolerance.","PeriodicalId":7975,"journal":{"name":"Applied Ecology and Environmental Research","volume":"23 2","pages":"2603-2619"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://doi.org/10.15666/aeer/2302_26032619","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147879022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PHYTOCHEMICAL CONSTITUENTS OF TICKBERRY (OSTEOSPEMUM MONILIFERUM L.) LEAF AND STEM EXTRACTS","authors":"Y. MHLOMI, Yougasphree Naidoo, Kuben Naidoo, Yaser Hassan Dewir, Salah El-Hendawy, T.S. ALSHAHRANI, R. H. Patil","doi":"10.15666/aeer/2303_38393860","DOIUrl":"https://doi.org/10.15666/aeer/2303_38393860","url":null,"abstract":"Tickberry (Osteospemum moniliferum) is renowned for its ethnomedicinal uses.This study aimed to evaluate the chemical composition of the leaves and stems of O. moniliferum.The phytochemical constituents were identified by phytochemical screening and gas chromatography-mass spectroscopy (GC-MS) techniques in hexane, and chloroform extracts.Fourier transmission infrared spectroscopy (FTIR) and Energy-dispersive X-ray (EDX) microanalysis were employed to identify the powdered samples' functional groups and elemental composition.The fluorescence of leaves and stem powders was examined using various chemical solvents.Phytochemical analysis of leaves and stems showed positive results for various phytochemical compounds.GC-MS revealed the presence of forty-seven compounds in leaf and stem extracts.FTIR analysis revealed different peak values with various functional groups in powdered samples, confirming the presence of alcohol, carboxylic acid, alkanes, amides, and alkyl halides.EDX microanalysis revealed the presence of carbon (65.07%), oxygen, chlorine, sodium, calcium, potassium, magnesium, and copper.Aluminium and silicon were only found in leaf samples.This study stands as the first report on the phytochemical constituents of this species.The identified phytochemicals indicate the potential therapeutic applications of O. moniliferum for a variety of ailments.These findings could pave the way leading to the establishment of potential drugs.","PeriodicalId":7975,"journal":{"name":"Applied Ecology and Environmental Research","volume":"23 3","pages":"3839-3860"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://doi.org/10.15666/aeer/2303_38393860","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147907959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"STUDY ON THE PHYSIOLOGICAL AND EMOTIONAL RECOVERY EFFECT OF MENTHA SPICATA L. TO COLLEGE STUDENTS UNDER VISUAL-OLFACTORY INTERACTION","authors":"Xiaodong Zhang, Huajun Chen, Yu Cai, Rong Yin, Wanshan Ning","doi":"10.15666/aeer/2306_1181511832","DOIUrl":"https://doi.org/10.15666/aeer/2306_1181511832","url":null,"abstract":"","PeriodicalId":7975,"journal":{"name":"Applied Ecology and Environmental Research","volume":"23 6","pages":"11815-11832"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://doi.org/10.15666/aeer/2306_1181511832","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147909540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"STUDY ON THE DIFFERENCES IN PHOTOSYNTHETIC CHARACTERISTICS AND LEAF ANATOMICAL STRUCTURE AMONG VARIOUS SPECIES OF ORMOSIA PLANTS","authors":"G.H. HE, Jintian Tang, Xue-Long Li, Lu Liu, Xiaojie Cai, Shengjun Feng, Rong Zou, Zhenxu Deng, Yanlin Luo, Lihong Gao, Xiaochun Wei","doi":"10.15666/aeer/2302_36033621","DOIUrl":"https://doi.org/10.15666/aeer/2302_36033621","url":null,"abstract":"In order to explore the photosynthetic characteristics and leaf anatomical structure of the Ormosia genus, a series of measurements were taken on three endangered species: Ormosia xylocarpa, Ormosia henryi and Ormosia microphylla.These measurements included the light response curve, the CO2 response curve, the leaf anatomical structure, the leaf epidermal characteristics and the chlorophyll content.The aim of this study was to identify the factors influencing plant photosynthesis.Results showed: (1) The three Ormosia species were light-favoring tree species.O. henryi had strong photosynthetic characteristics, and the maximum photosynthetic rate (Pmax) was 15.1354 molms, which was much higher than the other two Ormosia species.Among the three species of Ormosia, O. henryi has the strongest weak light utilization ability and CO2 utilization rate, and O. xylocarpa has a strong CO2 utilization range.(2) Among the three Ormosia species, O. xylocarpa had the thickest leaves, but the thickness of palisade tissue was O. henryi > O. xylocarpa > O. microphylla.(3) The single stomatal area of O. henryi was significantly higher than that of O. xylcarpa and O. microphylla, and the stomatal density of O. xylcarpa was significantly higher than that of O. henryi and O. microphylla.(4) The Chl a of the three Ormosia species was higher than Chl b, which was similar to the results of photosynthetic characteristics.The Chl a, Chl b and Chl (a+b) of O. henryi were significantly higher than those of O. xylocarpa and O. microphylla.The Car content of O. microphylla was significantly lower than that of O. henryi and O. xylocarpa.In conclusion; the leaf microstructure, chlorophyll content and epidermal characteristics of the three Ormosia species are significant importance with regard to their photosynthetic capacity.During the cultivation process, increasing CO2 concentration in the short term can increase the photosynthetic rate of the aforementioned three Ormosia species; in seedling management, appropriate increase in light conditions for Ormosia plants has been demonstrated to facilitate their growth.","PeriodicalId":7975,"journal":{"name":"Applied Ecology and Environmental Research","volume":"23 2","pages":"3603-3621"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://doi.org/10.15666/aeer/2302_36033621","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147879891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IMPACT OF NANOPARTICLES ON THE ANAEROBIC DIGESTION CHARACTERISTICS OF ENROFLOXACINCONTAINING MANURE OF LIVESTOCK AND POULTRY AND ANALYSIS OF MICROBIAL COMMUNITY EVOLUTION","authors":"Xiaofei Zhen, Ru Jiao, Hongbin Zhan, W.B. WU, Lei Feng","doi":"10.15666/aeer/2303_38213838","DOIUrl":"https://doi.org/10.15666/aeer/2303_38213838","url":null,"abstract":"","PeriodicalId":7975,"journal":{"name":"Applied Ecology and Environmental Research","volume":"23 3","pages":"3821-3838"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://doi.org/10.15666/aeer/2303_38213838","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147331592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A STUDY ON CIRCULAR CONSUMPTION BEHAVIOR ADDRESSING CONSUMERS’ UPSTREAM AND DOWNSTREAM ACTIVITIES IN PAKISTAN","authors":"Hassan Shams, Mohd Nizam Ab Rahman, Hawa Hishamuddin","doi":"10.15666/aeer/2306_1044310459","DOIUrl":"https://doi.org/10.15666/aeer/2306_1044310459","url":null,"abstract":"This study explores circular consumption behavior from the consumers' perspective.Consequently, this perspective is essential for connecting upstream and downstream circular activities, as consumers acquire products upstream and can contribute to closing the consumption loop through downstream participation.In this context, R-strategies play a key role when applied by consumers.A circular consumption behavior framework has been proposed, which is grounded in three consumer decisions: (1) purchase, (2) usage, and (3) post-use activities.This framework integrates these decisions with circular 8R-strategies connected to both upstream and downstream processes.For this purpose, data were collected from 528 respondents from Pakistan.The assessment focused on consumers' perspectives, 8R-strategies, and their mean responses.The overall mean score for circular consumption behaviors was found to be 3.00, suggesting a neutral to slightly low level of engagement among respondents.This study provides valuable insights into consumer-driven circular consumption behavior, which can support the establishment of a circular network.","PeriodicalId":7975,"journal":{"name":"Applied Ecology and Environmental Research","volume":"23 6","pages":"10443-10459"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://doi.org/10.15666/aeer/2306_1044310459","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147896637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BENEFICIAL EFFECTS OF MILD WATER DEFICIENCY ON GROWTH AND YIELD IN THE SEEDLING STAGE OF SOYBEAN","authors":"Mengjie Wang, Quansheng Chen, Ru Jia, Lei Zhou, C. WANG, Xijun Jin, Yancai Zhang, Liang Cao, Mengxia Wang, Chunyuan Ren","doi":"10.15666/aeer/2301_215234","DOIUrl":"https://doi.org/10.15666/aeer/2301_215234","url":null,"abstract":"This experiment was conducted at Heilongjiang Bayi Agricultural University in China, using Sui Nong 26 soybeans and negative pressure water supply equipment.The study set different water control periods (V1, R1, and R5 stages) and varying degrees of water stress (normal, mild, moderate, severe) to analyze soybean yield, nutrient uptake, growth, and physiological and biochemical responses to drought and water compensation.The aim was to establish high-quality, high-yield, water-saving cultivation techniques for dryland soybeans.Results showed that increasing water stress decreased soybean plant height and stem diameter.At the V1 and R1 stages, root length initially decreased and then increased, while drought stress at R1 and R5 stages inhibited dry matter accumulation.Severe drought stress significantly reduced dry matter compared to mild stress.Mild drought stress at the seedling stage increased dry matter accumulation, but severe stress hindered recovery after rehydration.During the V1 stage, mild drought stress increased Pn, Tr, and Gs by 7.9%, 16.4%, and 1.0%, respectively.In the R1 stage, drought stress reduced Pn, Gs, Ci, and Tr, with greater reductions under more severe stress.Post-rehydration, Pn, Gs, and Ci under mild stress were higher than CK.At the R5 stage, rehydration failed to restore photosynthetic capacity.Mild drought stress increased grain yield by 5.0% at the seedling stage but reduced it during flowering and bulging stages, also increasing grain protein content while decreasing lipid content.","PeriodicalId":7975,"journal":{"name":"Applied Ecology and Environmental Research","volume":"23 1","pages":"215-234"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://doi.org/10.15666/aeer/2301_215234","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147880161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"COMPARATIVE STUDY ON LEAF ANATOMICAL STRUCTURE AND PHOTOSYNTHETIC CHARACTERISTICS OF WILD AND RETROGRESSION POPULATIONS OF PAPHIOPEDILUM EMERSONII","authors":"Xiaojie Cai, Lihong Gao, Sam Yang, Lu Liang, Fengshou Tang, Yebin Jiang, Rong Zou, Xiaochun Wei","doi":"10.15666/aeer/2306_1091310926","DOIUrl":"https://doi.org/10.15666/aeer/2306_1091310926","url":null,"abstract":"This study systematically compared the differences in photosynthetic physiological characteristics and leaf anatomical structure between the population of the original habitat and the regression population of Paphiopedilum emersonii.The results showed that the in situ plants showed significant advantages in photosynthetic performance, including higher net photosynthetic rate, maximum photosynthetic rate (Pmax), light saturation point (LSP) and apparent quantum yield (AQY), but their chlorophyll content was lower than that of the regression plants.The anatomical structure showed that the leaves of the regression plants were thicker, and the thickness of palisade tissue and sponge tissue and stomatal area were significantly larger than those of the regression plants, which was positively correlated with their higher chlorophyll content.It is worth noting that photosynthetic parameters (Pmax, LSP, AQY) were significantly positively correlated with pigment parameters (Car/Chl a + b) and upper epidermis thickness (UET).Based on these findings, it is recommended to select a moist habitat with shading when returning to the introduction to alleviate photoinhibition and maintain water balance, thereby increasing the survival rate of the returning plants.","PeriodicalId":7975,"journal":{"name":"Applied Ecology and Environmental Research","volume":"23 6","pages":"10913-10926"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://doi.org/10.15666/aeer/2306_1091310926","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147877708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"EFFECTS OF DIFFERENT SHADING CONDITIONS ON THE GROWTH, PHOTOSYNTHETIC CHARACTERISTICS AND LEAF STRUCTURE OF FIRMIANA KWANGSIENSIS SAPLINGS","authors":"Yujie Yang, Rong Zou, Jianmin Tang, Pan Li, Shenlong Zhu, Xiaochun Wei","doi":"10.15666/aeer/2301_819837","DOIUrl":"https://doi.org/10.15666/aeer/2301_819837","url":null,"abstract":"To determine the optimal light environment for Firmiana kwangsiensis saplings, the growth characteristics and physiological traits of the saplings in environments shaded in different ways were compared with those in the full light environment as a control in this paper.The study revealed that F. kwangsiensis saplings showed significantly higher ground diameter, radiation area of the root, and fresh weight under 25% shading rate.The chlorophyll a, chlorophyll b, and total amount and ratio of both chlorophylls displayed a decreasing trend with the reduction of shading intensity.The photosynthetic physiological parameters, including maximum net photosynthetic rate (Pmax) and net photosynthetic rate (Pn), etc. of F. kwangsiensis saplings under 25% shading condition were higher than that in other treatments.The density of stomata, palisade tissue, spongy tissue, and leaf thickness of leaves increased with higher light intensity.The leaves of saplings under full light condition displayed a \"double-layer palisade tissue\" structure with drought-resistant properties.The results of correlation analysis showed that photosynthetic physiological characteristics and leaf anatomical structure had the greatest influence on the growth of F. kwangsiensis saplings.In summary, F. kwangsiensis saplings thrive in shaded environments, with a 25% shading being particularly optimal for their growth.However, it is worth noting that this plant is vulnerable to drought stress, and as such, it is crucial to ensure proper water management when introducing and cultivating it.","PeriodicalId":7975,"journal":{"name":"Applied Ecology and Environmental Research","volume":"23 1","pages":"819-837"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://doi.org/10.15666/aeer/2301_819837","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147878056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}