CONTACT TOXICITY AND ANTIFEEDANT ACTIVITIES OF RHODODENDRON YUNNANENSE EXTRACTS AGAINST STORAGE DISEASE AND PESTS OF TRADITIONAL CHINESE MEDICINES

IF 0.7 4区环境科学与生态学 Q4 ECOLOGY

Applied Ecology and Environmental Research Pub Date : 2026-01-01 DOI:10.15666/aeer/2403_42794297

X.X. WU, G.F. LI, Y.R. HE, H.Q. ZHU, Y.Q. GAO, W. G. Su

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Abstract

This study evaluated the contact-killing and anti-feeding activities of Yunnan Rhododendron (Rhododendron yunnanense) extracts and their isolated compounds against pests threatening stored traditional Chinese medicinal herbs. Extracts were prepared using methanol, 99% ethanol, petroleum ether, and deesterified methanol, followed by liquid-liquid extraction and chromatographic purification. Bioassays targeted Rhyzopertha dominica, Stegobium paniceum, and Ditylenchus dipsaci, with toxicity assessed via topical application and Tween 80 suspension methods, while anti-feeding activity was tested using flour-based assays. Key results showed that de-esterified methanol leaf extracts yielded the highest paste (31.25%), followed by 99% ethanol leaf extracts (30.58%). The 99% ethanol leaf extract exhibited the strongest contact-killing activity, with LD50 values of 15.76 μg/adult (R. dominica), 14.69 μg/adult (S. paniceum), and 0.74 mg/mL (D. dipsaci). Among isolated compounds, 4-methoxy-7-hydroxycoumarin demonstrated remarkable anti-feeding effects (75.24% refusal rate at 2.5 mg/mL), while apocynin showed potent contact toxicity (LD50 = 6.53 μg/adult for R. dominica). These findings highlight Yunnan Rhododendron. (R. yunnanense) as a promising source of natural insecticides for eco-friendly pest management in herbal storage. Further research should explore mechanistic pathways and field applicability.

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云南杜鹃提取物对中药贮藏病虫害的接触毒性及拒食活性研究

研究了云南杜鹃（Rhododendron yunnanense）提取物及其分离化合物对储存药害虫的杀虫和抗食活性。以甲醇、99%乙醇、石油醚、脱酯化甲醇为萃取剂，进行液液萃取、色谱纯化。生物实验以鼠尾蛇、虎尾蛇和二叉蛇为目标，通过外用和Tween 80悬浮液法评估毒性，用面粉法测试抗摄食活性。关键结果表明，脱酯化甲醇叶提取物的膏体率最高（31.25%），其次是99%乙醇叶提取物（30.58%）。99%乙醇叶提取物的接触杀虫活性最强，其LD50值分别为15.76 μg/成虫、14.69 μg/成虫和0.74 mg/mL。其中，4-甲氧基-7-羟基香豆素具有显著的抗取食作用（2.5 mg/mL拒取率为75.24%），罗布麻素具有较强的接触毒性（对家蝇的LD50 = 6.53 μg/成虫）。这些发现突出了云南杜鹃属植物。（R. yunnanense）是一种很有前途的天然杀虫剂，可用于中草药储存库的生态有害生物管理。进一步的研究应探索机理途径和野外适用性。

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来源期刊

Applied Ecology and Environmental Research ECOLOGY-ENVIRONMENTAL SCIENCES

CiteScore

1.40

自引率

14.30%

发文量

104

审稿时长

14 months

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