ROLE AND MECHANISM OF GMHORSTS GENE ON DROUGHT STRESS TOLERANCE OF SOYBEAN

IF 0.7 4区环境科学与生态学 Q4 ECOLOGY

Applied Ecology and Environmental Research Pub Date : 2025-01-01 DOI:10.15666/aeer/2302_26032619

Zhikang Li

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Abstract

With the frequent occurrence of global drought, some countries and regions are facing reduced soybean production.Therefore, in order to increase soybean yield and ensure global food security, the influence and mechanism of soybean GmHORSTs gene on its drought stress tolerance are analyzed, in order to provide support for improving soybean drought resistance.The experimental results indicated that after drought stress, the fresh weight, dry weight, and water content of pTRV-GmHORSTs were 0.90 g, 0.48 g, and 36.7%,respectively, significantly lower than that of pTRV-00.The content of malondialdehyde and H2O2 were both higher than in pTRV-00, with values of 0.41 umlo/g FW and 13.69 umlo/g, respectively.The fresh weight, dry weight, and water content of GmHORSTs overexpressed plants after drought stress were 1.51 g, 0.59 g, and 61.2%, respectively, all higher than those of wild plants, while their H2O2 and propylene glycol contents were lower than those of wild plants.Under drought stress, the withering degree of pTRV-GmHORSTs was higher than that of normal plants, while the withering degree of over-expressed plants was significantly lower than that of wild plants.In addition, under drought stress conditions, the stomatal conductance of pTRV-GmHORSTs was 19.7 um 2 , which was higher than that of pTRV-00.The stomatal conductance of over-expressed plants was 3.9 um 2 , which was lower than that of wild plants.The abscisic acid content of pTRV-GmHORSTs was 45.3 ng/mL, which was lower than that of pTRV-00.The abscisic acid content of over-expressed plants was 84.8 ng/mL and 84.7 ng/mL, both higher than that of wild plants.The above results indicate that the GmHORSTs gene can increase stomatal closure by increasing leaf abscisic acid content, thereby reducing transpiration and improving soybean drought tolerance.

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gmhorsts基因在大豆抗旱性中的作用及机制

随着全球干旱的频繁发生，一些国家和地区面临大豆减产。因此，为了提高大豆产量，保障全球粮食安全，分析大豆GmHORSTs基因对其抗旱性的影响及其机制，为提高大豆抗旱性提供支持。结果表明，干旱胁迫后，pTRV-GmHORSTs的鲜重、干重和含水量分别为0.90 g、0.48 g和36.7%，显著低于pTRV-00；丙二醛和H2O2含量均高于pTRV-00，分别为0.41 umlo/g FW和13.69 umlo/g。干旱胁迫后GmHORSTs过表达植株鲜重、干重和含水量分别为1.51 g、0.59 g和61.2%，均高于野生植株，H2O2和丙二醇含量低于野生植株。干旱胁迫下，pTRV-GmHORSTs的枯萎程度高于正常植株，而过表达植株的枯萎程度显著低于野生植株。此外，干旱胁迫条件下，pTRV-GmHORSTs的气孔导度为19.7 μ m 2，高于pTRV-00。过表达植株的气孔导度为3.9 μ m 2，低于野生植株。pTRV-GmHORSTs的脱落酸含量为45.3 ng/mL，低于pTRV-00。过表达植株脱落酸含量分别为84.8 ng/mL和84.7 ng/mL，均高于野生植株。上述结果表明，GmHORSTs基因可以通过增加叶片脱落酸含量来促进气孔关闭，从而减少蒸腾作用，提高大豆的抗旱性。

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来源期刊

Applied Ecology and Environmental Research ECOLOGY-ENVIRONMENTAL SCIENCES

CiteScore

1.40

自引率

14.30%

发文量

104

审稿时长

14 months

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