Biotechnology annual review最新文献_第3页

Advances in antibody manufacturing using mammalian cells. 利用哺乳动物细胞制造抗体的研究进展。

Biotechnology annual review Pub Date : 2007-01-01 DOI: 10.1016/S1387-2656(07)13005-2

K John Morrow

{"title":"Advances in antibody manufacturing using mammalian cells.","authors":"K John Morrow","doi":"10.1016/S1387-2656(07)13005-2","DOIUrl":"https://doi.org/10.1016/S1387-2656(07)13005-2","url":null,"abstract":"In this review, we describe recent advances in antibody processing technology including: (1) development of proprietary cell lines; (2) improved expression systems optimized by selective technologies to boost underperformers; (3) improved protein-free and serum-free culture media; and (4) attention to glycosylation and other post-translational modifications. Advances in computer technology and sophisticated redesign of bioreactors have been major contributors to the dramatic improvements in antibody yields that have been documented in the last decade. Disposable bioreactor components are now widespread, resulting in improved yields, better quality product and lower costs for producers. Downstream innovations include (1) disposable devices for clarification and purification, (2) improved resins and ligands, and (3) new designs of hardware for improved performance. While there are numerous factors contributing to the increased yields that have been obtained, the most sustained of these is the introduction of disposable technologies on both the upstream and the downstream ends of the process. With the continuing introduction of improved computer technology and technological innovation, there is every reason to believe that quality and quantity of antibody products will continue to improve in the coming years, and supply will be adequate to meet the forthcoming needs of the industry.","PeriodicalId":79566,"journal":{"name":"Biotechnology annual review","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1387-2656(07)13005-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40979842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 23

Production of plasmid DNA for pharmaceutical use. 医药用质粒DNA的生产。

Biotechnology annual review Pub Date : 2007-01-01 DOI: 10.1016/S1387-2656(07)13008-8

Carsten Voss

引用次数: 26

Porous silicon protein microarray technology and ultra-/superhydrophobic states for improved bioanalytical readout. 多孔硅蛋白微阵列技术和提高生物分析读数的超/超疏水状态。

Biotechnology annual review Pub Date : 2007-01-01 DOI: 10.1016/S1387-2656(07)13007-6

Anton Ressine, György Marko-Varga, Thomas Laurell

{"title":"Porous silicon protein microarray technology and ultra-/superhydrophobic states for improved bioanalytical readout.","authors":"Anton Ressine, György Marko-Varga, Thomas Laurell","doi":"10.1016/S1387-2656(07)13007-6","DOIUrl":"https://doi.org/10.1016/S1387-2656(07)13007-6","url":null,"abstract":"One attractive method for monitoring biomolecular interactions in a highly parallel fashion is the use of microarrays. Protein microarray technology is an emerging and promising tool for protein analysis, which ultimately may have a large impact in clinical diagnostics, drug discovery studies and basic protein research. This chapter is based upon several original papers presenting our effort in the development of new protein microarray chip technology. The work describes a novel 3D surface/platform for protein characterization based on porous silicon. The simple adjustment of pore morphology and geometry offers a convenient way to control wetting behavior of the microarray substrates. In this chapter, an interesting insight into the surface role in bioassays performance is made. The up-scaled fabrication of the novel porous chips is demonstrated and stability of the developed supports as well as the fluorescent bioassay reproducibility and data quality issues are addressed. We also describe the efforts made by our group to link protein microarrays to matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), suggesting porous silicon as a convenient platform for fast on-surface protein digestion protocols linked to MS-readout. The fabrication of ultra- and superhydrophobic states on porous silicon is also described and the utilization of these water-repellent properties for a new microscaled approach to superhydrophobic MALDI-TOF MS target anchor chip is covered.","PeriodicalId":79566,"journal":{"name":"Biotechnology annual review","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1387-2656(07)13007-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40979845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 57

Engineering, expression, purification, and production of recombinant thermolysin. 重组热溶酶的工程、表达、纯化和生产。

Biotechnology annual review Pub Date : 2007-01-01 DOI: 10.1016/S1387-2656(07)13003-9

Kuniyo Inouye, Masayuki Kusano, Yasuhiko Hashida, Masashi Minoda, Kiyoshi Yasukawa

{"title":"Engineering, expression, purification, and production of recombinant thermolysin.","authors":"Kuniyo Inouye, Masayuki Kusano, Yasuhiko Hashida, Masashi Minoda, Kiyoshi Yasukawa","doi":"10.1016/S1387-2656(07)13003-9","DOIUrl":"https://doi.org/10.1016/S1387-2656(07)13003-9","url":null,"abstract":"Thermolysin [EC 3.4.24.27] is a thermostable neutral zinc metalloproteinase originally identified in the culture broth of Bacillus thermoproteolyticus Rokko. Since the discovery in 1962, the enzyme has been extensively studied regarding its structure and catalytic mechanism. Today, thermolysin is a representative of zinc metalloproteinase and an attractive target in protein engineering to understand the catalytic mechanism, thermostability, and halophilicity. Thermolysin is used in industry, especially for the enzymatic synthesis of N-carbobenzoxy L-Asp-L-Phe methyl ester (ZDFM), a precursor of an artificial sweetener, aspartame. Generation of genetically engineered thermolysin with higher activity in the synthesis of ZDFM has been highly desired. In accordance with the expansion of studies on thermolysin, various strategies for its expression and purification have been devised and successfully used. In this review, we aim to outline recombinant thermolysins associated with their engineering, expression, purification, and production.","PeriodicalId":79566,"journal":{"name":"Biotechnology annual review","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1387-2656(07)13003-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40977676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 37

Ganoderma lucidum and its pharmaceutically active compounds. 灵芝及其药物活性化合物。

Biotechnology annual review Pub Date : 2007-01-01 DOI: 10.1016/S1387-2656(07)13010-6

Bojana Boh, Marin Berovic, Jingsong Zhang, Lin Zhi-Bin

引用次数: 460

Biotechnology, health, and peace. Foreword. 生物技术、健康与和平。前言。

Biotechnology annual review Pub Date : 2007-01-01 DOI: 10.1016/S1387-2656(07)13013-1

Ole D Mjøs

引用次数: 0

Citric acid production. 柠檬酸生产。

Biotechnology annual review Pub Date : 2007-01-01 DOI: 10.1016/S1387-2656(07)13011-8

Marin Berovic, Matic Legisa

引用次数: 222

Toward a new era in sequencing. 迈向测序的新时代。

Biotechnology annual review Pub Date : 2007-01-01 DOI: 10.1016/S1387-2656(07)13001-5

Burkhard Ziebolz, Marcus Droege

引用次数: 7

Potentials of phenolic molecules of natural origin and their derivatives as anti-HIV agents. 天然来源的酚类分子及其衍生物作为抗hiv药物的潜力。

Biotechnology annual review Pub Date : 2007-01-01 DOI: 10.1016/S1387-2656(07)13009-X

Mahmud Tareq Hassan Khan, Arjumand Ather

{"title":"Potentials of phenolic molecules of natural origin and their derivatives as anti-HIV agents.","authors":"Mahmud Tareq Hassan Khan, Arjumand Ather","doi":"10.1016/S1387-2656(07)13009-X","DOIUrl":"https://doi.org/10.1016/S1387-2656(07)13009-X","url":null,"abstract":"Identification of phenolic compounds and their derivatives interfering the several steps of the viral life cycle of the human immunodeficiency virus type 1 (HIV-1) is focused for the development of novel molecules for the treatment of AIDS. Several phenolic compounds isolated and characterized from natural sources have been studied in detail and found to exhibit inhibitory effects against different steps of the HIV-1 life cycle, including virus-cell fusion and virus absorption, reverse transcription, integration (IN) and proteolytic cleavage. In the review, we are summarizing some strong evidences demonstrating several phenolic molecules and their derivatives from natural sources display promising anti-HIV-1 activities. The anti-HIV compounds have been organized in this review according to their mechanism of action in the life cycle of HIV. We also mentioned some findings using in silico approaches, like virtual screening, docking, neural network, etc., and even the chemogenomics and/or functional genomics approaches could be useful for the quick identifying promising new lead anti-HIV molecules without having any other unwanted pharmacological effects. Plants having large amount of phenolic compounds, can be considered as strong sources of molecules for the treatment of HIV-1. Despite the continuous advances made in antiretroviral combination therapy, AIDS has become the leading cause of death in Africa and the fourth worldwide. Today, many research groups are exploring the bio- and chemo-diversity of the plant kingdom to find new and better anti-HIV drugs with novel mechanisms of action.","PeriodicalId":79566,"journal":{"name":"Biotechnology annual review","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1387-2656(07)13009-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40979847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

Trends in the development and application of functional biomembrane surfaces. 功能性生物膜表面的发展与应用趋势。

Biotechnology annual review Pub Date : 2006-01-01 DOI: 10.1016/S1387-2656(06)12004-9

Tzong-Hsien Lee, Marie-Isabel Aguilar

引用次数: 5