Molecular pathology : MP最新文献_第5页

Epstein-Barr virus encoded latent membrane protein 1 (LMP1) and TNF receptor associated factors (TRAF): colocalisation of LMP1 and TRAF1 in primary EBV infection and in EBV associated Hodgkin lymphoma. eb病毒编码的潜伏膜蛋白1 (LMP1)和TNF受体相关因子(TRAF): LMP1和TRAF1在原发性EBV感染和EBV相关霍奇金淋巴瘤中的共定位

Molecular pathology : MP Pub Date : 2003-06-01 DOI: 10.1136/mp.56.3.156

G Siegler, E Kremmer, R Gonnella, G Niedobitek

{"title":"Epstein-Barr virus encoded latent membrane protein 1 (LMP1) and TNF receptor associated factors (TRAF): colocalisation of LMP1 and TRAF1 in primary EBV infection and in EBV associated Hodgkin lymphoma.","authors":"G Siegler, E Kremmer, R Gonnella, G Niedobitek","doi":"10.1136/mp.56.3.156","DOIUrl":"https://doi.org/10.1136/mp.56.3.156","url":null,"abstract":"Aims: Epstein-Barr virus (EBV) immortalises B cells in vitro and is associated with several malignancies. Most phenotypic effects of EBV are mediated by latent membrane protein 1 (LMP1), which interacts with tumour necrosis factor receptor associated factors (TRAFs) to activate NF-kappaB. This study examines TRAF1 and LMP1 expression in EBV associated lymphoproliferations.Methods: TRAF1 expression was investigated in 26 Hodgkin lymphomas (HL; 18 EBV+, eight EBV-), seven EBV+ Burkitt lymphomas (BL), two infectious mononucleosis (IM) tonsils, and lymphoreticular tissue from eight chronic virus carriers. Seven anaplastic large cell lymphomas and 10 follicular B cell lymphomas were also studied. Colocalisation of TRAF1 and LMP1 was studied by immunofluorescent double labelling and confocal laser microscopy.Results: TRAF1 colocalises with LMP1 in EBV infected cells in IM. EBV positive lymphocytes from chronic virus carriers were negative for TRAF1 and LMP1. In HL biopsies, TRAF1 was strongly expressed independently of EBV status, whereas all BL cases were TRAF1-. In EBV+ HL cases, TRAF1 colocalised with LMP1. Eight of 10 follicular lymphomas expressed TRAF1 in centroblast-like cells. Four of seven anaplastic large cell lymphomas weakly expressed TRAF1.Conclusions: These results suggest that in non-neoplastic lymphocytes, TRAF1 expression is dependent on the presence of LMP1, and that in IM B cells in vivo, LMP1 associated signalling pathways are active. In HL, TRAF1 is expressed independently of EBV status, probably because of constitutive NF-kappaB activation. The function of TRAF1 in HL remains to be determined.","PeriodicalId":79512,"journal":{"name":"Molecular pathology : MP","volume":"56 3","pages":"156-61"},"PeriodicalIF":0.0,"publicationDate":"2003-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1187311/pdf/mp56000156.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22416174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Three novel PAX6 mutations in patients with aniridia. 无虹膜患者的三个新的PAX6突变。

Molecular pathology : MP Pub Date : 2003-06-01 DOI: 10.1136/mp.56.3.180

W Zumkeller, U Orth, A Gal

引用次数: 16

Molecular Cytogenetics. Protocols and Applications 分子细胞遗传学。协议和应用

Molecular pathology : MP Pub Date : 2003-06-01 DOI: 10.1136/mp.56.3.190

J. Crocker

引用次数: 2

Genetic vulnerability following traumatic brain injury: the role of apolipoprotein E. 外伤性脑损伤后的遗传易感性:载脂蛋白E的作用。

Molecular pathology : MP Pub Date : 2003-06-01 DOI: 10.1136/mp.56.3.132

N Nathoo, R Chetty, J R van Dellen, G H Barnett

引用次数: 93

Molecular changes in the Ki-ras and APC genes in primary colorectal carcinoma and synchronous metastases compared with the findings in accompanying adenomas. Ki-ras和APC基因在原发性结直肠癌和同步转移中的分子变化与伴发腺瘤的比较

Molecular pathology : MP Pub Date : 2003-06-01 DOI: 10.1136/mp.56.3.137

P Zauber, M Sabbath-Solitare, S P Marotta, D T Bishop

{"title":"Molecular changes in the Ki-ras and APC genes in primary colorectal carcinoma and synchronous metastases compared with the findings in accompanying adenomas.","authors":"P Zauber, M Sabbath-Solitare, S P Marotta, D T Bishop","doi":"10.1136/mp.56.3.137","DOIUrl":"https://doi.org/10.1136/mp.56.3.137","url":null,"abstract":"Aims: To compare the molecular genetic changes in the Ki-ras and adenomatous polyposis coli (APC) genes between colorectal carcinomas and synchronous metastases, and then to compare and contrast those changes with previously reported changes in the two genes between these carcinomas and accompanying adenomas. This expanded comparison would provide greater understanding of the progression of molecular changes in neoplastic tissue during the development of malignancy from a benign adenoma to carcinoma and then to metastatic spread of the malignancy.Methods: DNA was extracted from paraffin wax embedded tissue. This was followed by polymerase chain reaction and gel electrophoresis for mutations in the Ki-ras gene using single stranded conformational polymorphism analysis. Amplification of a CA repeat marker was used to assess loss of heterozygosity (LOH) at the APC gene.Results: The findings for the Ki-ras gene in 42 paired carcinomas and synchronous metastases were identical, regardless of whether or not the carcinoma and its companion adenoma had identical Ki-ras findings. The results of APC LOH for 39 paired carcinomas and synchronous metastases were also identical, whether or not the carcinoma and its companion adenoma had identical APC LOH findings. Results were uninformative for three pairs.Conclusions: With respect to these two genes, a carcinoma may be discordant from its companion adenoma, but the metastasis remains consistent with the colonic carcinoma.","PeriodicalId":79512,"journal":{"name":"Molecular pathology : MP","volume":"56 3","pages":"137-40"},"PeriodicalIF":0.0,"publicationDate":"2003-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1187308/pdf/mp56000137.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22415158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 59

Increased zinc finger protein zFOC1 transcripts in gastric cancer compared with normal gastric tissue. 与正常胃组织相比，胃癌中锌指蛋白zFOC1转录本增加。

Molecular pathology : MP Pub Date : 2003-06-01 DOI: 10.1136/mp.56.3.167

R L Stephen, J E Crabtree, T Yoshimura, C L Clayton, M F Dixon, P A Robinson

引用次数: 5

Measurement of thyroglobulin mRNA in peripheral blood as an adjunctive test for monitoring thyroid cancer. 外周血甲状腺球蛋白mRNA检测作为监测甲状腺癌的辅助检测。

Molecular pathology : MP Pub Date : 2003-06-01 DOI: 10.1136/mp.56.3.162

D Grammatopoulos, Y Elliott, S C Smith, I Brown, R J Grieve, E W Hillhouse, M A Levine, M D Ringel

{"title":"Measurement of thyroglobulin mRNA in peripheral blood as an adjunctive test for monitoring thyroid cancer.","authors":"D Grammatopoulos, Y Elliott, S C Smith, I Brown, R J Grieve, E W Hillhouse, M A Levine, M D Ringel","doi":"10.1136/mp.56.3.162","DOIUrl":"https://doi.org/10.1136/mp.56.3.162","url":null,"abstract":"Aims: Monitoring treated patients with thyroid cancer for recurrent or metastatic disease is currently based upon the serial measurement of circulating plasma thyroglobulin (Tg) concentrations. However, the clinical usefulness of Tg immunoassays is limited by poor sensitivity and interference from anti-Tg antibodies. This study investigated whether the detection of Tg mRNA in peripheral blood, using reverse transcriptase polymerase chain reaction (RT-PCR), is of value in the biochemical surveillance of patients with thyroid cancer.Methods: RNA was extracted from peripheral blood of five normal controls, six patients with abnormal thyroid function tests, and 28 patients who had undergone thyroidectomy for well differentiated thyroid cancer. From each, an 87 bp product from base pair 262 to 348 in the cDNA sequence of the thyroglobulin gene was amplified by RT-PCR.Results: Tg mRNA was detected in normal individuals and patients with thyroid cancer. In the group of patients studied, identification of metastatic thyroid tissue by radioiodine scanning correlated better with Tg mRNA assay results than with serum Tg concentrations (accuracy 84% v 75%). No interference from circulating Tg antibodies was apparent. In patients studied prospectively over a 12 month period, there was a significant correlation between detectable Tg mRNA in peripheral blood and the presence or absence of metastatic disease, as demonstrated by radioiodine scanning.Conclusions: These results suggest that detection of Tg mRNA in blood is a more sensitive marker for metastatic thyroid disease than Tg immunoassay, and appears to be unaffected by the presence of circulating anti-Tg antibodies.","PeriodicalId":79512,"journal":{"name":"Molecular pathology : MP","volume":"56 3","pages":"162-6"},"PeriodicalIF":0.0,"publicationDate":"2003-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1187312/pdf/mp56000162.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22416176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 43

PCR analysis in archival postmortem tissues. 档案尸体组织PCR分析。

Molecular pathology : MP Pub Date : 2003-06-01 DOI: 10.1136/mp.56.3.184

S Bonin, F Petrera, B Niccolini, G Stanta

{"title":"PCR analysis in archival postmortem tissues.","authors":"S Bonin, F Petrera, B Niccolini, G Stanta","doi":"10.1136/mp.56.3.184","DOIUrl":"https://doi.org/10.1136/mp.56.3.184","url":null,"abstract":"Background: Formalin fixed and paraffin wax embedded tissues of necropsy origin are an important source for molecular analysis especially in rare diseases, neuropathology, or molecular epidemiology studies. Because of DNA degradation, only short sequences can be amplified from this type of tissue, very often less than 100 bases. This poses problems because studies on polymorphism and mutations occurring in large genes often require the analysis of long sequences.Methods: The development of a simple treatment to obtain longer fragments of DNA for the analysis of archival postmortem paraffin wax embedded tissues.Results: It was possible to amplify longer sequences ranging up to 300 bases from postmortem tissues, with no modification to the usual DNA extraction procedures. To obtain longer stretches of DNA, a pre-PCR restoration treatment was required, by filling single strand breaks, followed by a vigorous denaturation step.Conclusions: The development of this simple treatment allowed the analysis of longer fragments of DNA obtained from archival postmortem paraffin wax embedded tissues.","PeriodicalId":79512,"journal":{"name":"Molecular pathology : MP","volume":"56 3","pages":"184-6"},"PeriodicalIF":0.0,"publicationDate":"2003-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1187316/pdf/mp56000184.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"22416178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 133

No evidence of tumour cells in blood of patients with glioma. 神经胶质瘤患者血液中没有肿瘤细胞的证据。

Molecular pathology : MP Pub Date : 2003-06-01 DOI: 10.1136/mp.56.3.187

C Böhm, H Wassmann, W Paulus

引用次数: 12

Abstracts from the Second International Workshop on the CCN Family of Genes 第二届CCN基因家族国际研讨会摘要

Molecular pathology : MP Pub Date : 2003-04-01 DOI: 10.1136/mp.56.2.65

引用次数: 0