Antimicrobial Resistance and Infection Control最新文献

{"title":"Correction: Healthcare workers' knowledge, attitude and practices on infection prevention and control in the context of the COVID-19 pandemic at the Faranah regional hospital and associated healthcare centers, Guinea.","authors":"Lena Landsmann, Anna Borodova, Carlos Rocha, Aziz Amadou Diallo, Kamis Mamadou Diallo, Matthias Borchert, Mardjan Arvand, Mamadou Diallo, Rebekah R Wood, Sophie A Müller","doi":"10.1186/s13756-025-01566-x","DOIUrl":"https://doi.org/10.1186/s13756-025-01566-x","url":null,"abstract":"","PeriodicalId":7950,"journal":{"name":"Antimicrobial Resistance and Infection Control","volume":"14 1","pages":"43"},"PeriodicalIF":4.8,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12057052/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and identification of Klebsiella pneumoniae phage ΦK2046: optimizing its antibacterial potential in combination with chlorhexidine.","authors":"Panjie Hu, Yao Sun, Zeyong Zhong, Sichen Liu, Deyi Zhao, Weijun Chen, Ying Zhang, Zhexiao Ma, Jianming Cao, Tieli Zhou","doi":"10.1186/s13756-025-01548-z","DOIUrl":"https://doi.org/10.1186/s13756-025-01548-z","url":null,"abstract":"<p><strong>Background: </strong>Hospital-acquired infections (HAIs) significantly increase morbidity and mortality worldwide, with Klebsiella pneumoniae (K. pneumoniae) being a leading HAI pathogen requiring targeted eradication in healthcare settings. The growing bacterial tolerance to chemical disinfectants, like chlorhexidine, highlights an urgent need for novel disinfection strategies. Bacteriophages, which employ unique mechanisms to lyse bacteria, offer a potential solution. Combining phages with disinfectants could reduce the use of chemical agents and delay the development of bacterial resistance. However, the use of phages for contamination control in clinical environments remains underexplored.</p><p><strong>Methods: </strong>ΦK2046 was isolated from hospital wastewater and characterized by transmission electron microscopy, one-step growth curve, optimal multiplicity of infection, and stability analysis. Whole-genome sequencing was performed to identify the genomic characteristics of ΦK2046. The antibacterial and antibiofilm effects of ΦK2046 combined with chlorhexidine were assessed through growth curves, time-kill assays, crystal violet staining, and scanning electron microscopy. A contaminated medical device model was established to assess the ΦK2046-chlorhexidine combination's biofilm reduction efficacy, and different dosing sequences and timing intervals were evaluated for their impact on biofilms formed on urinary catheters.</p><p><strong>Results: </strong>ΦK2046, characterized by a short latency period, strong environmental stability, safety, and tolerance to chlorhexidine, significantly enhanced the antibacterial and antibiofilm effects of chlorhexidine against FK2046, and reduce the emergence of resistant strains. In contaminated medical device models, the combination of ΦK2046 and chlorhexidine diminished bacterial load and biofilm formation on surfaces. A \"phage-first\" dosing sequence, particularly with a 90-min interval before chlorhexidine treatment, showed superior efficacy in biofilm reduction.</p><p><strong>Conclusions: </strong>This study, using ΦK2046 as an example, demonstrates the potential of phages to enhance the antibacterial and antibiofilm effects of chlorhexidine and their feasibility in medical device disinfection. This innovative approach not only improves chlorhexidine's disinfecting power but also effectively tackles the issue of reduced susceptibility of K. pneumoniae to chlorhexidine. The research advances the development and application of phage-based disinfectants and lays a foundation for establishing a phage library with adjuvant properties for disinfectants.</p>","PeriodicalId":7950,"journal":{"name":"Antimicrobial Resistance and Infection Control","volume":"14 1","pages":"42"},"PeriodicalIF":4.8,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12053862/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143969799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High rate of multi-drug resistant Escherichia coli isolated from patients with urinary tract infections in Ifakara-Tanzania: implications for empirical antibiotic treatment guidelines and stewardship programs.","authors":"Magreth Erick Macha, Philipp Kohler, Anja Bösch, Honorathy Msami Urassa, Weihong Qi, Salome N Seiffert, Sabine Haller, Erin West, Maja Weisser Rohacek, Baharak Babouee Flury","doi":"10.1186/s13756-025-01557-y","DOIUrl":"https://doi.org/10.1186/s13756-025-01557-y","url":null,"abstract":"<p><strong>Background: </strong>Antimicrobial resistance (AMR) in Sub-Saharan Africa is a significant health threat, with limited data guide treatment. This study investigates multi-drug resistant Escherichia coli in urinary tract infections (UTIs) in rural Tanzania to guide empirical treatment strategies.</p><p><strong>Methods: </strong>A cross-sectional prospective study of adults with UTIs was conducted at St. Francis Regional Referral Hospital in Ifakara, Tanzania, from September 2021 to August 2023. Urine culture isolates underwent routine diagnostics in Tanzania and E. coli isolates underwent whole-genome sequencing in Switzerland.</p><p><strong>Results: </strong>Of 1055 patients, 248 (23.5%) had positive urine cultures, with E. coli as predominant pathogen (n = 87; 55.7%). Extended-spectrum beta-lactamase-producing E. coli (ESBL-E) was identified in 20 (23.0%) isolates, primarily sequence type ST167 carrying CTX-M-27. All ESBL-E cases (20/20, 100.0%) and half of non-ESBL-E cases (29/58, 50.0%) received empiric antibiotics to which the isolates were documented as resistant. ESBL-E showed higher resistance to cotrimoxazole (100.0%) and ciprofloxacin (90.0%) latter recommended for complicated UTIs in Tanzania's Standard Treatment Guidelines (STG) compared to non-ESBL-E. All ESBL-E isolates were susceptible to nitrofurantoin, as recommended by STG for uncomplicated UTIs, and fosfomycin showed potential alternative for complicated cases.</p><p><strong>Conclusion: </strong>Nearly one-quarter of E. coli isolates causing UTIs were ESBL-E, predominantly ST167 harboring bla_CTX-M-27. Notably, nitrofurantoin remained effective for uncomplicated UTIs, similarly, fosfomycin emerged as a viable alternative. However, ciprofloxacin, despite being recommended in local guidelines for complicated UTIs, showed no efficacy. The genetic similarity between human and environmental isolates underscores the critical need for a One Health approach to tackle antimicrobial resistance (AMR) in the region.</p>","PeriodicalId":7950,"journal":{"name":"Antimicrobial Resistance and Infection Control","volume":"14 1","pages":"41"},"PeriodicalIF":4.8,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12046935/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143968859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Management and microbial monitoring of final rinse water for flexible endoscopes in 290 hospitals in Jiangsu Province, China: a multicenter cross-sectional study.","authors":"Zhanjie Li, Bo Liu, Qingtang Zhu, Zijun Ge, Feng Zang, Wensen Chen, Yongxiang Zhang, Xiafen Ding, Jing Ding, Weihong Zhang","doi":"10.1186/s13756-025-01560-3","DOIUrl":"https://doi.org/10.1186/s13756-025-01560-3","url":null,"abstract":"<p><strong>Background: </strong>There is a lack of a universally accepted standard or guideline for the frequency of disinfection in purified water pipelines. Furthermore, there is no standardized method for detecting microorganisms in the final rinse water utilized for endoscope cleaning.This study aims to examine the current management and microbial monitoring practices concerning the final rinse water used for flexible endoscope cleaning in medical institutions.</p><p><strong>Methods: </strong>A questionnaire was designed using a convenience sampling method to gather data on the maintenance and microbial monitoring of final rinse water for flexible endoscopes in 290 medical institutions across Jiangsu Province, China.</p><p><strong>Results: </strong>Purified water is used for endoscope rinsing by 93.45% of institutions, with 78.62% employing centralized water supply. Membrane filtration devices at the terminal are installed by 82.07%, mainly with a 0.2μm pore size (76.47%), and are replaced quarterly (32.77%). Disinfection devices are present at 52.76% of terminals, with varied disinfection frequencies; chlorine-containing disinfectants (48.15%) and peracetic acid (34.92%) are predominant. Inadequate disinfection, filter membrane neglect, sampling contamination, and biofilm formation are identified as reasons for non-compliant final rinse water. Actions include filter replacement, pipeline disinfection, and flushing. Microbial mornitoring occurs quarterly (70.96%), with faucet outlets as primary sampling sites. Standards are based on 10cfu/100ml (87.58%), using membrane filtration (40.81%) and nutrient agar plates (82.72%). A cultivation period of 2 days predominated (72.43%), with a temperature range of 35-37°C (76.47%).</p><p><strong>Conclusion: </strong>While purified water and terminal filters are common for final rinsing of endoscopes, there is variation in maintenance and supply line disinfection. Current microbiological methods' reliability is considered low, necessitating further research to establish unified standards for effective endoscope final rinse water management and monitoring.</p>","PeriodicalId":7950,"journal":{"name":"Antimicrobial Resistance and Infection Control","volume":"14 1","pages":"40"},"PeriodicalIF":4.8,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12044760/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143959197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Duration of hospitalization increases the risk for long-term carriage of linezolid-resistant enterococci in critically ill patients.","authors":"Vera Rauschenberger, Heike Claus, Charlotte Polzin, Vera Blaschke, Stefanie Kampmeier","doi":"10.1186/s13756-025-01551-4","DOIUrl":"https://doi.org/10.1186/s13756-025-01551-4","url":null,"abstract":"<p><strong>Background: </strong>Enterococci are gut commensal microorganisms, which can however cause life-threatening infections especially in patients suffering from intestinal barrier disorders. Treatment of these enterococcal infections is challenging due to a variety of intrinsic and acquired antibiotic resistances. In this context, linezolid is applied as last-resort antibiotic. Our study aimed at determining linezolid-resistant enterococci (LRE) long-term carriage (≥ 10 weeks), since this is a risk factor for the development of LRE infection.</p><p><strong>Methods: </strong>In a one-year cohort study, all patients on hemato-oncology, intensive and intermediate care units were screened for LRE. To determine the molecular epidemiology, all detected LRE isolates were subjected to whole genome sequencing-based typing to investigate whether in-host selection or pathogen transmission was causative for LRE occurrence. Clinical and demographic data were recorded to identify risk factors for LRE clearance and persistence.</p><p><strong>Results: </strong>Long-term LRE carriage was identified in 7 of 46 (15%) patients. Duration of hospitalization differed significantly between LRE persistence (mean: 110 days; range 28-225 days) and clearance group (mean: 53 days; range 5-213 days). LRE strains mostly exhibited a high genetic core genome diversity, indicating that transmission events played a minor role.</p><p><strong>Conclusions: </strong>Our study shows that the duration of hospitalization increases the risk for long-term carriage of LRE. In contrast to other multi drug resistant bacteria, LRE carriage was rarely caused by transmission events. Thus, future infection prevention measures should focus on antimicrobial stewardship approaches next to classical hygiene strategies.</p>","PeriodicalId":7950,"journal":{"name":"Antimicrobial Resistance and Infection Control","volume":"14 1","pages":"39"},"PeriodicalIF":4.8,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12042563/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of standard precautions and unrestricted movements of carbapenemase-producing Enterobacterales (CPE) carriers on CPE transmission in a nursing home in Singapore: a prospective cohort study.","authors":"Kyaw Zaw Linn, Xiaowei Huan, Pei Yun Hon, Sharifah Farhanah Syed Husen, Natascha May Thevasagayam, Oon Tek Ng, Shawn Vasoo, Moi Lin Ling, Dale Fisher, Kalisvar Marimuthu","doi":"10.1186/s13756-025-01554-1","DOIUrl":"https://doi.org/10.1186/s13756-025-01554-1","url":null,"abstract":"<p><strong>Background: </strong>In 2018, Singapore's National Infection Prevention & Control Committee (NIPC) recommended standard precautions and unrestricted movements for CPE carriers in nursing homes.</p><p><strong>Objective: </strong>This study investigates the short-term impact of this intervention on CPE transmission in a nursing home in Singapore.</p><p><strong>Methods: </strong>We conducted a prospective cohort study between 1st April and 11th July 2019 in a 255-bedded nursing home in Singapore. Stool samples from residents and environmental samples from sink strainers in the residents' bedrooms, bathrooms, and lavatories, and shower drain traps in bathrooms were collected at baseline, week 2, week 8, and week 12 and tested for CPE. We performed whole genomic sequencing (WGS) to find out if there was any bacterial or plasmid linkage among the residents and between the residents and environment.</p><p><strong>Results: </strong>A total of 32 residents, including six known CPE carriers, were recruited and completed the three-month follow-up visits. Of the six known CPE carriers, five tested negative for CPE, while one consistently tested positive for CPE throughout the study. Of the 28 sink strainers, six (21.43%) were positive for CPE. CPE was not detected in any shower drain trap throughout the study. Only one resident acquired CPE at week 12. WGS analysis of available CPE isolates showed no bacterial or plasmid linkage between residents or between residents and the environment.</p><p><strong>Conclusions: </strong>Standard precautions and unrestricted movement of CPE carriers may be sufficient to control CPE transmission in the nursing home setting. Larger studies with more extensive environmental sampling and longer follow-up periods are needed to confirm this.</p>","PeriodicalId":7950,"journal":{"name":"Antimicrobial Resistance and Infection Control","volume":"14 1","pages":"38"},"PeriodicalIF":4.8,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12039280/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143965331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Antimicrobial stewardship in the community setting: a qualitative exploratory study.","authors":"Rose I Okonkwo, Henry Ndukwe, Gary Grant, Sohil Khan","doi":"10.1186/s13756-025-01556-z","DOIUrl":"https://doi.org/10.1186/s13756-025-01556-z","url":null,"abstract":"","PeriodicalId":7950,"journal":{"name":"Antimicrobial Resistance and Infection Control","volume":"14 1","pages":"35"},"PeriodicalIF":4.8,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12016336/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143965388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of fungi and their antifungal and disinfectant resistance in hospital environments: insights into combating nosocomial mycoses.","authors":"Soudabeh Ghodsi, Mahnaz Nikaeen, Shima Aboutalebian, Rasoul Mohammadi, Hossein Mirhendi","doi":"10.1186/s13756-025-01558-x","DOIUrl":"https://doi.org/10.1186/s13756-025-01558-x","url":null,"abstract":"<p><strong>Background: </strong>Fungal infections are increasingly recognized as a global health concern, contributing to considerable morbidity and mortality in hospital settings. This underscores the urgent need for infection prevention and control in healthcare facilities to protect vulnerable patients from the risk of acquiring invasive fungal diseases (IFDs). Given the critical role of transmission-based precautions in limiting the spread of filamentous fungi responsible for IFDs, this study was conducted to explore the potential role of the hospital environment in the dissemination of these infections.</p><p><strong>Methods: </strong>A total of 83 samples were collected from the air and surface of exhaust vents in the intensive care units (ICUs) of hospitals in Isfahan, Iran, to assess the presence and diversity of fungal species. Susceptibility testing against antifungal agents, including commonly used drugs and disinfectants, was performed on the identified fungal isolates. Furthermore, the antifungal resistance profiles of isolates from clinical IFD cases were compared with those of environmental isolates.</p><p><strong>Results: </strong>Fungi were detected in 45% of air samples and 100% of exhaust vent samples, with Aspergillus species being the most commonly identified genus. Mucorales were also found in 17% of exhaust vent samples. Aspergillus spp. and Rhizopus spp. showed the highest resistance to Amphotericin B, and a considerable proportion of these isolates exhibited simultaneous resistance to disinfectants. A similar antifungal resistance profile was noted between A. flavus and some R. arrhizus isolates from both environmental and clinical samples.</p><p><strong>Conclusions: </strong>The findings of this study indicate that the hospital environment, particularly exhaust vents, may act as a significant reservoir for causative agents of IFDs. This highlights the importance of environmental surveillance in preventing and controlling nosocomial fungal infections.</p>","PeriodicalId":7950,"journal":{"name":"Antimicrobial Resistance and Infection Control","volume":"14 1","pages":"37"},"PeriodicalIF":4.8,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12020322/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143954068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing bedding hygiene in long-term care facilities: investigating the impact of multilevel antimicrobial polymers (MAP-1) on bacterial and MDRO reduction.","authors":"Awais Farid, Wei Han, Joseph K C Kwan, King Lun Yeung","doi":"10.1186/s13756-025-01555-0","DOIUrl":"https://doi.org/10.1186/s13756-025-01555-0","url":null,"abstract":"<p><strong>Objective: </strong>This study aims to assess the bactericidal efficacy of Multilevel Antimicrobial Polymer (MAP-1) on standard bedsheets in Long-term care facility (LTCF). The research quantifies total viable bacteria and MRSA counts and evaluates the percentage difference between treated and control bedding material over a one-week period.</p><p><strong>Design: </strong>A double-blind interventional, double cross-over study.</p><p><strong>Setting: </strong>Haven of Hope Woo Ping Care and Attention Home in Sai Kung, Hong Kong.</p><p><strong>Methods: </strong>Over an 8-week period, bedding materials from residents' rooms were sampled, totalling 288 samples from 96 bedsheets, with half treated with MAP-1 and the remaining serving as controls. MAP-1, developed at The Hong Kong University of Science and Technology, incorporates USFDA and USEPA-approved polymers. Sampling procedures adhered to standardized protocols, and bacterial counts were determined using culture methods. Data analysis employed t-tests and ANOVA to compare microbial loads between the control and treatment groups, with statistical significance set at p < 0.05.</p><p><strong>Results: </strong>The study revealed a significant reduction in total viable bacteria and MRSA counts on bedsheets treated with MAP-1. Noteworthy reductions of 80.37% for total bacteria and 87.31% for MRSA at the end of seven-day use, in the intervention group compared to the control. These reductions were statistically significant across all four observation periods and among both male and female residents.</p><p><strong>Conclusion: </strong>The study establishes the bactericidal efficacy of MAP-1 on standard bedsheets, showcasing its potential in diminishing total bacterial counts and MRSA contamination. These results hold promise for enhancing infection control practices and promoting improved sanitary conditions within healthcare settings.</p>","PeriodicalId":7950,"journal":{"name":"Antimicrobial Resistance and Infection Control","volume":"14 1","pages":"36"},"PeriodicalIF":4.8,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12020249/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143959614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of a national repository of Fourier-transform infrared spectroscopy spectra enables fast detection of silent outbreaks and prevention of spread of new antibiotic-resistant sequence types.","authors":"Mor N Lurie-Weinberger, Elizabeth Temkin, Ophir Kastel, Moshe Bechor, Darya Bychenko-Banyas, Reut Efrati-Epchtien, Gabrielle D Levi, Nadya Rakovitsky, Alona Keren-Paz, Yehuda Carmeli","doi":"10.1186/s13756-025-01546-1","DOIUrl":"https://doi.org/10.1186/s13756-025-01546-1","url":null,"abstract":"<p><strong>Background: </strong>The reference microbiology laboratory of Israel's National Institute for Antibiotic Resistance and Infection Control has established a national repository of isolates analyzed by Fourier-transform infrared (FTIR) spectroscopy and their spectra. Healthcare institutions send antibiotic-resistant isolates as part of outbreak investigation, periodic nation-wide collection of specific species, or point prevalence studies. Here, we describe the use of a national FTIR repository to detect the emergence and spread of new sequence types and resistance mechanisms.</p><p><strong>Methods: </strong>Using FTIR, we produced dendrograms of outbreaks and periodic country-level dendrograms of isolates from selected species. When FTIR identified new clusters that were distinct from previously characterized clusters, they were investigated further by whole genome sequencing.</p><p><strong>Results: </strong>FTIR analysis uncovered two clones new to Israel: NDM-5-producing E. coli ST650 harboring a novel plasmid, and NDM-producing K. pneumoniae ST307.</p><p><strong>Conclusions: </strong>Establishing regional or national FTIR repositories could serve as a simple and effective tool for early detection of new antibiotic-resistant clones.</p>","PeriodicalId":7950,"journal":{"name":"Antimicrobial Resistance and Infection Control","volume":"14 1","pages":"34"},"PeriodicalIF":4.8,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12013074/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}