Cytokines, cellular & molecular therapy最新文献_第4页

Retrovirus-mediated gene transfer of granulocyte colony-stimulating factor receptor (G-CSFR) cDNA into MDS cells and induction of their differentiation by G-CSF. 逆转录病毒介导的粒细胞集落刺激因子受体(G-CSFR) cDNA基因转染MDS细胞及G-CSF诱导MDS细胞分化

Cytokines, cellular & molecular therapy Pub Date : 2000-06-01 DOI: 10.1080/13684730050515787

S Nakamura, K Ohnishi, H Yoshida, K Shinjo, A Takeshita, K Tohyama, R Ohno, Y Koide

{"title":"Retrovirus-mediated gene transfer of granulocyte colony-stimulating factor receptor (G-CSFR) cDNA into MDS cells and induction of their differentiation by G-CSF.","authors":"S Nakamura, K Ohnishi, H Yoshida, K Shinjo, A Takeshita, K Tohyama, R Ohno, Y Koide","doi":"10.1080/13684730050515787","DOIUrl":"https://doi.org/10.1080/13684730050515787","url":null,"abstract":"Myelodysplastic syndromes (MDS) are clonal disorders in which the proper differentiation of hematopoietic stem cells is impaired. There is no effective treatment for this stem cell disorder at present. In an attempt to find a new strategy that promotes the differentiation of MDS blast cells, we tried retroviral transduction of granulocyte colony-stimulating factor receptor (G-CSFR) into an interleukin-3-dependent MDS cell line, MDS-L, since expression of G-CSFR is known to be essential for the differentiation of myeloid progenitor cells and this expression is impaired in most MDS cells. Ectopic expression of human G-CSFR cDNA in MDS-L cells gave rise to granulocytic differentiation by G-CSF stimulation. G-CSF caused the transformants expressing G-CSFR to display a morphological characteristic of mature granulocytes, upregulated CD11b on the cell surface, and improved NBT reduction activity. These results demonstrate that MDS-L cells ecopically expressing G-CSFR are induced to granulocytic differentiation upon exposure to G-CSF, and shed light on the molecular mechanisms of maturation arrest in MDS cells.","PeriodicalId":79485,"journal":{"name":"Cytokines, cellular & molecular therapy","volume":"6 2","pages":"61-70"},"PeriodicalIF":0.0,"publicationDate":"2000-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/13684730050515787","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21931975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Effect of interferon-alpha on CD20 antigen expression of B-cell chronic lymphocytic leukemia. 干扰素- α对b细胞慢性淋巴细胞白血病CD20抗原表达的影响

Cytokines, cellular & molecular therapy Pub Date : 2000-06-01 DOI: 10.1080/13684730050515804

S Sivaraman, P Venugopal, R Ranganathan, C G Deshpande, X Huang, A Jajeh, S A Gregory, T O'Brien, H D Preisler

{"title":"Effect of interferon-alpha on CD20 antigen expression of B-cell chronic lymphocytic leukemia.","authors":"S Sivaraman, P Venugopal, R Ranganathan, C G Deshpande, X Huang, A Jajeh, S A Gregory, T O'Brien, H D Preisler","doi":"10.1080/13684730050515804","DOIUrl":"https://doi.org/10.1080/13684730050515804","url":null,"abstract":"Chimeric CD20 monoclonal antibody as alternative therapy in relapsed low-grade non-Hodgkin's lymphoma (NHL) has produced responses in nearly 50% of patients. Augmenting CD20 expression on tumor cells and/or inducing its expression may increase the cell kill and effectiveness of antibody therapy. Peripheral blood lymphocytes from 19 patients with B-cell chronic lymphocytic leukemia (B-CLL) were incubated in vitro in the presence of interferon-alpha (IFN-alpha) (500 U/ml and 1,000 U/ml) for 24 and 72 hours. The effect on CD20 expression was studied by flow cytometry. The differences in the percentage positivity, the mean fluorescence intensity (MFI), and the product of percentage positivity and MFI were used to assess upregulation. There was a significant upregulation of CD20 expression on B cells seen at both concentrations after 24-hour priming (p < 0.01). B-CLL cells cultured for 72 hours in the presence of IFN-alpha also showed upregulation of CD20 expression; however, the degree of upregulation was much lower than that seen at 24 hours. There was no statistically significant increase in CD20 antigen expression on normal lymphocytes following cytokine exposure. These results suggest that IFN-alpha priming may augment the effectiveness of antibody therapy by directly upregulating CD20 antigen expression in addition to its indirect action through effector cells of the host.","PeriodicalId":79485,"journal":{"name":"Cytokines, cellular & molecular therapy","volume":"6 2","pages":"81-7"},"PeriodicalIF":0.0,"publicationDate":"2000-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/13684730050515804","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21931977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 35

Expression of HSV-TK suicide gene in primary T lymphocytes: the dog as a preclinical model. 单纯疱疹病毒- tk自杀基因在原代T淋巴细胞中的表达:犬临床前模型。

Cytokines, cellular & molecular therapy Pub Date : 2000-03-01 DOI: 10.1080/13684730050515886

E M Weissinger, M Franz, C Voss, C Bonini, E Kremmer, H J Kolb

{"title":"Expression of HSV-TK suicide gene in primary T lymphocytes: the dog as a preclinical model.","authors":"E M Weissinger, M Franz, C Voss, C Bonini, E Kremmer, H J Kolb","doi":"10.1080/13684730050515886","DOIUrl":"https://doi.org/10.1080/13684730050515886","url":null,"abstract":"Expression of suicide genes (e.g. herpes simplex virus thymidine kinase,HSV-TK) in T cells is an appealing approach to regulate graft-versus-host disease in adoptive immunotherapy. Here we report the optimization of retroviral infection of canine T cells. Canine T cells were stimulated either with phytohemagglutinin (PHA, 2 microg/ml) for 24-72 hours or with 100 U/ml interleukin-2 for seven days. Stimulated cells were co-cultivated with irradiated virus-producing cells. Transduction efficiencies ranged from 4% to 45% using PG13, a gibbon ape leukemia virus envelope (env) pseudotyped packaging cell line. Infection of cells with GPenvAM12, expressing the amphotropic Moloney murine leukemia virus env, did not yield a satisfactory percentage of transduced cells. Enrichment of transduced cells was performed using immunoselection, and gave a purity of up to 98%. Transfusion of 1 x 10(6) transduced cells per kilogram body weight showed that transduced cells could convert mixed chimerism to 100% and transfer immunity to a specific antigen. Transduced cells were repeatedly detected in peripheral blood and bone marrow by polymerase chain reaction with primers specific for the HSV-TK gene. We have demonstrated the feasibility of using the canine model to study gene therapy as a preclinical model.","PeriodicalId":79485,"journal":{"name":"Cytokines, cellular & molecular therapy","volume":"6 1","pages":"25-33"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/13684730050515886","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21810775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Hyperfractionated radiotherapy concomitant with cisplatin and granulocyte colony-stimulating factor (filgrastim) for laryngeal carcinoma. 高分割放疗联合顺铂和粒细胞集落刺激因子(非格昔汀)治疗喉癌。

Cytokines, cellular & molecular therapy Pub Date : 2000-03-01 DOI: 10.1080/13684730050515895

M Tejedor, J J Valerdi, F Arias, M A Dominguez, E Pruja, L Mendez, J J Illarramendi

{"title":"Hyperfractionated radiotherapy concomitant with cisplatin and granulocyte colony-stimulating factor (filgrastim) for laryngeal carcinoma.","authors":"M Tejedor, J J Valerdi, F Arias, M A Dominguez, E Pruja, L Mendez, J J Illarramendi","doi":"10.1080/13684730050515895","DOIUrl":"https://doi.org/10.1080/13684730050515895","url":null,"abstract":"An open-label, non-randomized study evaluated the feasibility and efficacy of filgrastim (recombinant methionyl human granulocyte colony-stimulating factor, r-metHuG-CSF) to prevent mucositis induced by accelerated hyperfractionated radiotherapy (1.6 Gy b.i.d., total dose 67.2 Gy in six weeks with a two-week split) and concomitant chemotherapy (cisplatin, 20 mg/m2/day, days 1-5 by continuous intravenous infusion) in patients with laryngeal carcinoma. Filgrastim 300 microg/day was administered on days 1, 3, and 5 in weeks 2-6 of radiotherapy, after the second fraction. Twenty patients (three stage II, six stage III, and eleven stage IV, according to AJCC) were enrolled in the trial. Oral mucosal toxicity was grade 2 in nine patients (45%), grade 3 in eight (40%), and grade 4 in three (15%). Severe hematological toxicity (WHO criteria) was uncommon. Nineteen patients (95%) completed the treatment in the planned time. Overall survival was 55% at three years. The administration of filgrastim with this regimen was feasible, and it appeared to reduce the severity and duration of mucositis induced by the combined treatment.","PeriodicalId":79485,"journal":{"name":"Cytokines, cellular & molecular therapy","volume":"6 1","pages":"35-9"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/13684730050515895","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21810776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Enhancing effects of co-transduction of both human erythropoietin receptor and c-kit cDNAs into hematopoietic stem/progenitor cells from cord blood on proliferation and differentiation of erythroid progenitors. 人促红细胞生成素受体和c-kit cdna共转导到脐带血造血干细胞/祖细胞对红细胞祖细胞增殖和分化的影响

Cytokines, cellular & molecular therapy Pub Date : 2000-03-01 DOI: 10.1080/13684730050515859

M S Dai, M C Heinrich, H E Broxmeyer, L Lu

{"title":"Enhancing effects of co-transduction of both human erythropoietin receptor and c-kit cDNAs into hematopoietic stem/progenitor cells from cord blood on proliferation and differentiation of erythroid progenitors.","authors":"M S Dai, M C Heinrich, H E Broxmeyer, L Lu","doi":"10.1080/13684730050515859","DOIUrl":"https://doi.org/10.1080/13684730050515859","url":null,"abstract":"Steel factor (SLF) and erythropoietin (Epo) play critical roles in erythropoiesis. To evaluate interactive effects of Epo and SLF receptors (R) in erythropoiesis, CD34+ and CD34 cord blood cells were transduced with human EpoR and c-kit cDNAs by retroviral mediated gene transfer. Erythroid (BFU-E) colonies derived from CD34+ or CD34 cells transduced with either the EpoR or c-kit gene were significantly increased in the presence of interleukin (IL)-3, granulocyte-macrophage colony-stimulating factor (GM-CSF), Epo, and different concentrations of SLF compared with that from mock transduced cells. This number was further enhanced by co-transduction of both genes. Enhancement was more apparent in the absence of SLF. Cell numbers in individual erythroid colonies were also significantly increased in cells transduced with both genes compared with cells transduced with a single gene. Short-term liquid culture showed that ex vivo expansion for five days and numbers of CD34+CD71+ cells in expanded cells from single CD34 cells co-transduced with both EpoR and c-kit genes were increased compared with those of EpoR or c-kit-transduced cells. These results demonstrate that co-transduction of both c-kit and EpoR enhances the proliferative capacity of erythroid progenitors under cytokine stimulation above that of single-gene transduced cells.","PeriodicalId":79485,"journal":{"name":"Cytokines, cellular & molecular therapy","volume":"6 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/13684730050515859","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21810772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

The role of interleukin-12 and interferon-gamma in GVHD and GVL. 白细胞介素-12和干扰素- γ在GVHD和GVL中的作用。

Cytokines, cellular & molecular therapy Pub Date : 2000-03-01 DOI: 10.1080/13684730050515903

Y G Yang

{"title":"The role of interleukin-12 and interferon-gamma in GVHD and GVL.","authors":"Y G Yang","doi":"10.1080/13684730050515903","DOIUrl":"https://doi.org/10.1080/13684730050515903","url":null,"abstract":"The production of interleukin (IL)-12 by antigen-presenting cells after antigen stimulation is a critical step for initiating antigen-specific cellular immune responses, and interferon (IFN)-gamma produced by natural killer cells and activated T cells is a potent mediator of IL-12 effect. However, recent studies have demonstrated that administration of exogenous IL-12 paradoxically inhibits antigen-specific immunity of T cells in vivo, including allogeneic, autoimmune, and viral antigen-initiated T-cell responses. Interestingly, these inhibitory effects are also mediated by IFN-gamma, whose production is induced by IL-12. Thus, IL-12, a potent immunostimulatory cytokine, can paradoxically lead to immunosuppression. Notably, this cytokine has been shown to preserve graft-versus-leukemia (GVL) effects of allogeneic CD8+ T cells while inhibiting graft-versus-host disease (GVHD) in murine allogeneic bone marrow transplantation models. This article will review recent studies concerning the effect of IL-12 and IFN-gamma on the development of GVHD and the induction of GVL effects, and discuss the possible mechanisms responsible for IL-12-mediated separation of GVL effects from the GVHD-promoting activity of allogeneic T cells.","PeriodicalId":79485,"journal":{"name":"Cytokines, cellular & molecular therapy","volume":"6 1","pages":"41-6"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/13684730050515903","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21810777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 15

Megakaryocyte growth and development factor (MGDF): an Mpl ligand and cytokine that regulates thrombopoiesis. 巨核细胞生长发育因子(megakyocyte growth and development factor, MGDF):一种调节血小板生成的Mpl配体和细胞因子。

Cytokines, cellular & molecular therapy Pub Date : 2000-03-01 DOI: 10.1080/13684730050515912

T A Neumann, M Foote

{"title":"Megakaryocyte growth and development factor (MGDF): an Mpl ligand and cytokine that regulates thrombopoiesis.","authors":"T A Neumann, M Foote","doi":"10.1080/13684730050515912","DOIUrl":"https://doi.org/10.1080/13684730050515912","url":null,"abstract":"Megakaryocyte growth and development factor (MGDF) is a ligand for the Mpl receptor related to thrombopoietin (TPO). MGDF stimulates megakaryocytopoiesis and thrombopoiesis, and is highly selective to cells bearing the Mpl receptor. Studies done in rodents, nonhuman primates, and humans have confirmed that MGDF can increase platelet counts in normal and chemotherapy- or radiotherapy-treated subjects. Platelet function and physiology remain normal after MGDF administration, with no effect on platelet aggregation. Pegylated recombinant human MGDF (PEG-rHuMGDF) was used clinically with initial success. Cancer patients receiving chemotherapy showed dose-dependent increases in platelet counts and increases in bone marrow megakaryocytes. Clinical development of PEG-rHuMGDF was halted owing to the formation of neutralizing antibodies in some patients and normal volunteers who received the drug. The application of exogenous recombinant Mpl ligands should be explored in the setting of randomized clinical trials and the findings extended to mobilization of CD34+ stem cells, ex vivo expansion techniques, and use in platelet abnormalities.","PeriodicalId":79485,"journal":{"name":"Cytokines, cellular & molecular therapy","volume":"6 1","pages":"47-56"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/13684730050515912","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21810778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

Level of interleukin-8 expression by metastatic human melanoma cells directly correlates with constitutive NF-kappaB activity. 转移性人黑色素瘤细胞中白细胞介素-8的表达水平与nf - κ b活性直接相关。

Cytokines, cellular & molecular therapy Pub Date : 2000-03-01 DOI: 10.1080/13684730050515868

S Huang, A DeGuzman, C D Bucana, I J Fidler

{"title":"Level of interleukin-8 expression by metastatic human melanoma cells directly correlates with constitutive NF-kappaB activity.","authors":"S Huang, A DeGuzman, C D Bucana, I J Fidler","doi":"10.1080/13684730050515868","DOIUrl":"https://doi.org/10.1080/13684730050515868","url":null,"abstract":"The purpose of this study was to determine whether constitutive NF-kappaB activity regulates the expression level of interleukin-8 (IL-8) in metastatic human melanoma cells. Cultures of metastatic human A375 melanoma cells expressed higher levels of IL-8 mRNA and protein than nonmetastatic A375 human melanoma cells. No discernible differences in IL-8 half-life were found between metastatic and nonmetastatic cells, but cells that overexpressed IL-8 had a higher transcription rate and increased IL-8 promoter activity. Analysis of the IL-8 promoter using deletion mutants revealed that the region within -133 was essential for constitutive IL-8 promoter activity and that mutation of NF-kappaB binding sites eliminated the constitutive IL-8 promoter activity. The activation of constitutive IL-8 transcription directly correlated with the level of constitutive NF-kappaB activity. Transfection of melanoma cells with a dominant-negative mutant IkappaBalpha expression vector (pLXSN-IkappaBalphaM) significantly decreased the level of constitutive NF-kappaB activity and expression of IL-8, demonstrating that constitutive NF-kappaB/relA activities contribute to overexpression of IL-8 in highly metastatic human melanoma cells.","PeriodicalId":79485,"journal":{"name":"Cytokines, cellular & molecular therapy","volume":"6 1","pages":"9-17"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/13684730050515868","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21810773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 62

Enhanced expression of the CXCR4 co-receptor in HIV-1-infected individuals correlates with the emergence of syncytia-inducing strains. CXCR4共受体在hiv -1感染个体中的增强表达与合胞诱导菌株的出现相关。

Cytokines, cellular & molecular therapy Pub Date : 2000-03-01 DOI: 10.1080/13684730050515877

R Manetti, L Cosmi, G Galli, F Annunziato, M Mazzetti, S Romagnani, E Maggi

{"title":"Enhanced expression of the CXCR4 co-receptor in HIV-1-infected individuals correlates with the emergence of syncytia-inducing strains.","authors":"R Manetti, L Cosmi, G Galli, F Annunziato, M Mazzetti, S Romagnani, E Maggi","doi":"10.1080/13684730050515877","DOIUrl":"https://doi.org/10.1080/13684730050515877","url":null,"abstract":"The aim of this study was to investigate the mechanisms responsible for the emergence in some HIV-1-infected individuals of highly aggressive, syncytia-inducing (SI) HIV-1 strains, which have been shown to use CXCR4 as co-receptor to enter target cells. To this end, the percentages of circulating CXCR4+CD4+ T cells were evaluated by flow cytometry in 39 untreated and 61 highly active antiretroviral therapy (HAART)-treated HIV-1-infected individuals in comparison with 35 HIV-1 seronegative subjects. Plasma viremia was also measured, and HIV primary isolates, from both untreated and HAART-treated HIV-1-infected subjects, were tested for the presence of SI strains. The results of this study showed enhanced proportions of CXCR4+CD4+ T cells in untreated patients in comparison with HAART-treated and healthy subjects. Furthermore, the results of a 12-month longitudinal study in a cohort of 11 patients undergoing HAART showed a significant reduction of CXCR4 expression after successful therapy. Finally, a significant positive correlation among the proportions of circulating CXCR4-expressing CD4+ T cells, plasma viremia, and the probability to isolate SI strains was found. These in vivo data are in keeping with previous in vitro results suggesting a bidirectional link between HIV-1 and CXCR4 expression on CD4+ T cells, and provide some clues to understanding the mechanisms exerting a selective pressure toward the emergence of SI strains.","PeriodicalId":79485,"journal":{"name":"Cytokines, cellular & molecular therapy","volume":"6 1","pages":"19-24"},"PeriodicalIF":0.0,"publicationDate":"2000-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/13684730050515877","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21810774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

ICAM-1 (CD54) expression on T lymphocytes and natural killer cells from umbilical cord blood: regulation with interleukin-12 and interleukin-15. ICAM-1 (CD54)在脐带血T淋巴细胞和自然杀伤细胞中的表达:白细胞介素-12和白细胞介素-15的调控

Cytokines, cellular & molecular therapy Pub Date : 2000-01-01 DOI: 10.1080/MCCM.6.4.161.164

S. Lin, D. Yan

{"title":"ICAM-1 (CD54) expression on T lymphocytes and natural killer cells from umbilical cord blood: regulation with interleukin-12 and interleukin-15.","authors":"S. Lin, D. Yan","doi":"10.1080/MCCM.6.4.161.164","DOIUrl":"https://doi.org/10.1080/MCCM.6.4.161.164","url":null,"abstract":"We investigated the effect of two immunoregulatory cytokines, interleukin (IL)-12 and IL-15, alone or in combination, on intercellular adhesion molecule 1 (ICAM-1, CD54) expression on mononuclear cells (MNC) obtained from umbilical cord blood (CB) and adult peripheral blood (APB). We established that (1) ICAM-1 expression was deficient on freshly isolated CB T and natural killer (NK) cells compared with that on adult cells; (2) ICAM-1 expression on T cells (CD3+/CD54+), but not on NK cells (CD16+/CD54+), was spontaneously upregulated after 7 days' culture in RPMI with 10% fetal calf serum (FCS) in the absence of cytokines, for CB and APB MNC alike; (3) removal of 10% FCS from the medium did not affect the spontaneous CD3+/CD54+ upregulation on APB MNC; (3) CB NK cells responded more readily to IL-12 and IL-15 than did APB NK cells in terms of ICAM-1 expression, while ICAM-1 expression on APB T cells, but not on CB T cells, could be enhanced with IL-12 plus IL-15; (4) the combination of IL-12 and IL-15 downregulated ICAM-1 expression on both CB and APB NK cells. Thus, we demonstrated the different patterns of ICAM-1 regulation by CB MNC and APB MNC in response to IL-12 and/or IL-15 and the differential effect of cytokines on the regulation of adhesion molecules on neonatal NK and T cells.","PeriodicalId":79485,"journal":{"name":"Cytokines, cellular & molecular therapy","volume":"6 4 1","pages":"161-4"},"PeriodicalIF":0.0,"publicationDate":"2000-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/MCCM.6.4.161.164","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"60766208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21