Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine最新文献_第3页

Comparative investigations of quality of erythrocyte concentrates in preservation media SAG-M, PAGGS-M and Adsol without and with leukocyte depletion. 红细胞浓缩物保存介质SAG-M、PAGGS-M和Adsol在无白细胞损耗和有白细胞损耗情况下的质量比较研究。

Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine Pub Date : 1997-01-01

H Bäumler, H Radtke, B Franz, U Tofoté, I Pawlow, H Kiesewetter

引用次数: 0

New definition and methods for isolation of the earliest peripheral blood-derived hematopoietic stem cells. 外周血源性造血干细胞的新定义和分离方法。

Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine Pub Date : 1997-01-01

R Huss

{"title":"New definition and methods for isolation of the earliest peripheral blood-derived hematopoietic stem cells.","authors":"R Huss","doi":"","DOIUrl":"","url":null,"abstract":"Peripheral blood contains transplantable pluripotent hematopoietic progenitor cells, which are defined by the expression of certain surface antigens, such as CD34, and the absence of lineage-specific markers (lin-). Here we describe CD34- DR- adherent growing hematopoietic progenitor precursors which were separated and isolated from peripheral blood by interleukin 6(IL-6)-mediated plastic adherence. These peripheral blood mononuclear cells attach to the plastic surface of tissue culture flasks in the presence of human recombinant IL-6 and assume fibroblast-like morphology. These adherent cells are CD34- and HLA-DR-, and respond to various growth factors with the expression of surface antigens, changes in morphology and the expression of cell cycle-controlling kinases. Stem cell factor induces differentiation of the CD34- DR- adherent cells to become nonadherent and to differentiate into CD34+, and eventually HLA-DR+ cells, and produce colony-forming units (CFU) in semi-solid agar. While IL-6 conveys adherence of these cells, the addition of stem cell factor induces differentiation into nonadherent cells. Our observations suggest the possibility of isolating a true hematopoietic stem cell before the level of CD34 and HLA-DR expression.","PeriodicalId":79439,"journal":{"name":"Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine","volume":"34 ","pages":"128-32"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20286812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Positive selection of CD34+ cells by immune affinity. 免疫亲和对CD34+细胞的阳性选择。

Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine Pub Date : 1997-01-01

P Höcker, I Kührer, N Worel, G Leitner, P Kalhs, H Greinix, K Gerhartl

引用次数: 0

[Cytokines and chemokines as inducers of nonhemolytic transfusion reactions after thrombocyte transfusion]. [细胞因子和趋化因子作为血小板输注后非溶血性输血反应的诱导剂]。

Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine Pub Date : 1997-01-01

H Klüter, D Wilhelm, S Bubel, A Fiebelkorn, H Kirchner

{"title":"[Cytokines and chemokines as inducers of nonhemolytic transfusion reactions after thrombocyte transfusion].","authors":"H Klüter, D Wilhelm, S Bubel, A Fiebelkorn, H Kirchner","doi":"","DOIUrl":"","url":null,"abstract":"Recently, the leukocyte-derived cytokines interleukin (IL)-1 beta, IL-6, IL-8, and tumor necrosis factor(TNF)-alpha were found in considerable concentrations in platelet concentrates (PC) involved in nonhemolytic transfusion reactions (NHTR). We investigated transfusion reactions in two study periods over the last 3 years. In the 1st period, PC were leukocyte-depleted by bedside filtration. In the 2nd period we performed prestorage leukocyte filtration of PC. Recipients who experienced a transfusion reaction were tested for total and specific IgE, and reactions were analyzed with regard to the main symptoms. In the supernatant of platelets involved, we analyzed concentrations of IL-1 beta, IL-6, IL-8, TNF-alpha, macrophage inflammatory protein(MIP)-1 alpha, and RANTES. The incidence for NHTR did not change with the kind of PC transfused (n = 44, 1.63% and n = 46, 1.56%; p = 0.84), but allergic reactions were reduced in the 2nd observation period (0.92% versus 0.51%; p = 0.02). IL-1 beta, IL-6, IL-8, TNF-alpha, and MIP-1 alpha were not detectable in the majority of all products. PC involved in allergic reactions contained significantly higher concentrations of RANTES. We found evidence that the proinflammatory chemokine RANTES, stored in the platelet alpha-granules, is involved in allergic transfusion reactions. In contrast, leukocyte-derived inflammatory cytokines were of minor relevance in the onset of NHTR.","PeriodicalId":79439,"journal":{"name":"Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine","volume":"34 ","pages":"100-4"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20346115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Effect of rhG-CSF on platelets using in vitro bleeding time and transcranial Doppler examination]. [利用体外出血时间和经颅多普勒检查rhG-CSF对血小板的影响]。

Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine Pub Date : 1997-01-01

D Söhngen, M Siebler, S Wienen, G Kobbe, K Hollmig, P Schneider, C Boogen, A Heyll, W Schneider

引用次数: 0

[Typing of thrombocytic antigens on the DNA level and their value in diagnosis of alloimmunothrombocytopenias]. 血小板抗原的DNA分型及其在同种异体免疫性血小板减少症诊断中的价值

Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine Pub Date : 1997-01-01

J Weinreich, A Schabel, U Sugg

引用次数: 0

[HBV-DNA positive findings in HBsAg negative blood donors and patients]. [HBsAg阴性献血者和患者的HBV-DNA阳性结果]。

Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine Pub Date : 1997-01-01

H Hennig, R H Dennin, D Haase, H Kirchner

引用次数: 0

Leuko-reduction using an integral Sepacell filter early (4 h, 24 h) or late (48 h, 120 h) after plateletpheresis does not affect platelet function. 血小板提取后早期(4小时、24小时)或后期(48小时、120小时)使用完整的Sepacell过滤器减少白细胞不影响血小板功能。

Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine Pub Date : 1997-01-01

T H Müller, S Schmidt, A Döscher, H Weiss, F Schunter

{"title":"Leuko-reduction using an integral Sepacell filter early (4 h, 24 h) or late (48 h, 120 h) after plateletpheresis does not affect platelet function.","authors":"T H Müller, S Schmidt, A Döscher, H Weiss, F Schunter","doi":"","DOIUrl":"","url":null,"abstract":"Leuco-reduction of aliquots of single-donor platelet concentrates with an integral Sepacell PLS-5A filter was performed 4, 24, and 48 h after apheresis including a control after 120 h (not recommended by the manufacturer) to evaluate the effect of both early and late filtration on the concentrates in a paired study. Highly efficient (> 2 log10) leuko-reduction was consistently observed for filtration any time after apheresis. Various proaggregatory stimuli were tested to determine the stimulus concentration (EC50 values) required for half-maximal aggregation of platelet samples (200 platelets/nl). Neither glycoprotein IIb/IIIa-dependent (thrombin-, collagen-, and APD-induced) nor glycoprotein Ib-mediated (ristocetin-induced) platelet function were affected by filtration 4, 24, 48, or 120 h after plateletpheresis. For single-donor plateletpheresis using a closed system with an integral Sepacell filter, our in vitro results suggest that the timing of leuko-reduction does not affect the platelet-dependent hemostatic qualities of the product.","PeriodicalId":79439,"journal":{"name":"Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine","volume":"34 ","pages":"95-9"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20286810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Transfusion Medicine 1996/7. Proceedings of the 29th Congress of the German Society of Transfusion Medicine and Immunohematology. Essen, 11-14 September 1996]. 输血医学1996/7。德国输血医学和免疫血液学学会第29届大会论文集。埃森，1996年9月11-14日]。

Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine Pub Date : 1997-01-01

引用次数: 0

[Rh-D genotyping for exon 2, 5 and 7 of German and Japanese blood donors with sequence specific polymerase chain reaction]. [用序列特异性聚合酶链反应对德国和日本献血者外显子2、5和7进行Rh-D基因分型]。

Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine Pub Date : 1997-01-01

J H Maas, T J Legler, R Lynen, V Blaschke, H Ohto, M Köhler

{"title":"[Rh-D genotyping for exon 2, 5 and 7 of German and Japanese blood donors with sequence specific polymerase chain reaction].","authors":"J H Maas, T J Legler, R Lynen, V Blaschke, H Ohto, M Köhler","doi":"","DOIUrl":"","url":null,"abstract":"RHD genotyping from fetal cells was applied for the detection of the RHD gene in the fetus of immunized Rh-D-negative women. Additionally, RHD genotyping was applied for the characterization of Rh-D variants. Although 44 nucleotide substitutions are known to code for 35 amino acid differences between the RHCE and the RHD gene, only a few polymorphisms have been investigated yet. We investigated 7 RHD-specific nucleotides on exons 2, 5, and 7 with sequence-specific primers and 1 nucleotide with ligation-based typing. All RHD genotyping results were correlated with serological results and established genotyping methods in 116 German and 98 Japanese blood donors, because different genetic sequences coding for Rh-D polypeptides have been described in different ethnic groups. Sequence-specific amplification of D-specific sequences was concordant with the serological result in all blood donors tested. However, ligation-based typing on exon 5 gave false-negative results in 7 donors. In summary, 5 new sequence-specific PCRs have been evaluated for further characterization of Rh-D variants. Furthermore, the methods described allow nested PCR and thus may help in determination of the fetal RhD status from maternal peripheral blood during pregnancy.","PeriodicalId":79439,"journal":{"name":"Beitrage zur Infusionstherapie und Transfusionsmedizin = Contributions to infusion therapy and transfusion medicine","volume":"34 ","pages":"203-9"},"PeriodicalIF":0.0,"publicationDate":"1997-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"20346631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0