International journal of biological research in pregnancy最新文献_第3页

Evaluation of a multicomponent vaccine in dogs 犬用多组分疫苗的评价

International journal of biological research in pregnancy Pub Date : 2017-07-26 DOI: 10.14419/IJBR.V5I2.7426

MD AL Nayem Chowdhury, D. Dighe, R. Velhankar, A. Sarkar, S. Nandi

{"title":"Evaluation of a multicomponent vaccine in dogs","authors":"MD AL Nayem Chowdhury, D. Dighe, R. Velhankar, A. Sarkar, S. Nandi","doi":"10.14419/IJBR.V5I2.7426","DOIUrl":"https://doi.org/10.14419/IJBR.V5I2.7426","url":null,"abstract":"The concept of combined viral and bacterial vaccines has been tried in cattle, canine and humans. Use of multicomponent vaccines has numerous advantages over individual vaccine. In this study, apparently healthy 8-9 weeks old 41 puppies were divided into 2 groups. 31 puppies (test group) were vaccinated subcutaneously with a commercially available multicomponent vaccine containing live attenuated strains of Canine Distemper virus, Canine Adenovirus type 2, Canine Parvo virus and Canine Parainfluenza virus and inactivated antigens of Leptospira canicola and Leptospira icterohemorrhagiae. 10 puppies were kept unvaccinated (control). Test group puppies were given a booster after 4 weeks of primary vaccination. Serum samples from both the groups were collected on 4 occasions (0th, 28th, 42nd and 90th day) and were tested for antibody titres against Canine Distemper virus and Canine Adenovirus type 2 by Serum Neutralization test, against Canine Parvovirus and Canine Parainfluenzavirus by Haemagglutination Inhibition test and against Leptospira canicola and L. icterohemorrhagiae by Microscopic Agglutination Test. A significant rise (P 0.5) in antibody titres was observed against all the antigens after vaccination. The control group did not show significant variation in the antibody titres. All puppies vaccinated subcutaneously with this vaccine did not evoke any local/ adverse reactions.","PeriodicalId":79223,"journal":{"name":"International journal of biological research in pregnancy","volume":"57 1","pages":"36-40"},"PeriodicalIF":0.0,"publicationDate":"2017-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78614919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effects of tributyltin chloride (TBTCl) antifouling biocide on adult males and females of brine shrimp (Artemia salina) 三丁基氯化锡(TBTCl)防污杀菌剂对雄性和雌性卤虾(Artemia salina)的影响

International journal of biological research in pregnancy Pub Date : 2017-05-23 DOI: 10.14419/IJBR.V5I1.7584

Najla Mohamed Abu Shaala, S. Zulkifli, A. Ismail, M. N. A. Azmai, F. Mohamat-Yusuff, H. Omar

{"title":"Effects of tributyltin chloride (TBTCl) antifouling biocide on adult males and females of brine shrimp (Artemia salina)","authors":"Najla Mohamed Abu Shaala, S. Zulkifli, A. Ismail, M. N. A. Azmai, F. Mohamat-Yusuff, H. Omar","doi":"10.14419/IJBR.V5I1.7584","DOIUrl":"https://doi.org/10.14419/IJBR.V5I1.7584","url":null,"abstract":"Elevation of tributyltin (TBT) concentration in marine environment could affect targeted and non-targeted organisms at any lifestage. The present study aimed to determine median lethal concentration (LC 50 ) and morphological effects of tributyltin chloride (TBTCl) on adult males and females of brine shrimp ( Artemia salina ). The adult males and females of A. salina were exposed to different concentration of TBTCl. Morphological condition of every A. salina individuals were observed under a microscope. Results showed the LC 50 of TBTCl among adult males of A. salina was 146.99 ng.L -1 and for the females was 94.72 ng.L -1 , respectively. The LC 50 of TBTCl was significantly different among different sexes. There was also a significant difference in some morphological characters of males and females exposed to different TBTCl concentrations. These morphological changes include their total length, head width, abdominal width, and tail width after the 24hr exposure to TBTCl. These results suggested that suspensions of the TBTCl were toxic to Artemia , most likely due to the formation of benign TBTCl aggregates in water. However, the mortality increased with extended exposure to 24hr. Highest mortality occurred at 200 ng.L -1 TBTCl; 43.33% for male and 90% for female (LC 50 <150 ng.L -1 ) for both. Depended on this the female was more sensitive for TBTCl toxicity test when compared to male. These effects were attributed to changes in morphological characteristics of the body A. salina .","PeriodicalId":79223,"journal":{"name":"International journal of biological research in pregnancy","volume":"9 1","pages":"30-35"},"PeriodicalIF":0.0,"publicationDate":"2017-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80419466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Analysis and cloning of CP 1102 gene isolated from a medicinal plant 药用植物cp1102基因的克隆与分析

International journal of biological research in pregnancy Pub Date : 2017-05-12 DOI: 10.14419/IJBR.V5I1.6361

H. Mubeen, S. Raza

引用次数: 0

Lactic acid bacteria and identification with PCR-DGGE 乳酸菌及其PCR-DGGE鉴定

International journal of biological research in pregnancy Pub Date : 2017-04-05 DOI: 10.14419/IJBR.V5I1.7392

Y. Biçer, G. Uçar

引用次数: 1

Bioprospective potentials of endophytic fungi penicillium SPP isolated from leaves of azadirachta indica (A. JUSS) 印楝叶内生真菌青霉SPP的生物前景研究

International journal of biological research in pregnancy Pub Date : 2017-02-26 DOI: 10.14419/IJBR.V5I1.6069

S. Abubakar, R. W. Ndana, A. Afolabi

{"title":"Bioprospective potentials of endophytic fungi penicillium SPP isolated from leaves of azadirachta indica (A. JUSS)","authors":"S. Abubakar, R. W. Ndana, A. Afolabi","doi":"10.14419/IJBR.V5I1.6069","DOIUrl":"https://doi.org/10.14419/IJBR.V5I1.6069","url":null,"abstract":"The focus of this research work was to isolates and identify endophytic fungi from young leaves and stems (twig) of Azadirachta indica, family (Meliaceae) for the production and assay of the produced secondary metabolites. Altogether 126 segments were used, of which 63 segments each from leaves and stems (twig) tissues were screened, using modified surface sterilization techniques. A total of 12 species of endophytic fungi were purely isolated, Penicillium spp was randomly selected for the extraction and evaluation of its secondary metabolites. The eluent collected from column chromatography mixture of ethylacetate and n- hexane (50:50v/v) was phytochemically screened, and the results showed the presence of saponins, flavonoids and phenols. Based on disc diffusion method of sensitivity, the eluent possessed some degree of antibacterial and antifungal activities. In addition, in-vitro antioxidant potentiality of the eluent was also evaluated using 2, 2- Diphenyl-1-picrylhydrazyl radical (DPPH, Sigma Aldrich). Statistical package for social science (SPSS 3/93) software was used to analyze the results using one way Analysis of Variance (ANOVA), which revealed no significant difference, on the effect of concentrations of eluent on test organisms but the eluent showed significant difference on scavenging free radicals at a critical value (p >0.05).","PeriodicalId":79223,"journal":{"name":"International journal of biological research in pregnancy","volume":"5 1","pages":"15-21"},"PeriodicalIF":0.0,"publicationDate":"2017-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81886673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Principal Component Analysis of Morphological and Yield Attributing Traits in Advanced Breeding Lines of Rice Grown at Rainfed Shallow Lowland Condition of West Bengalvangaru 西孟加拉万加鲁雨养浅低地水稻高级选育系形态性状和产量性状的主成分分析

International journal of biological research in pregnancy Pub Date : 2017-01-01 DOI: 10.24247/IJASROCT201732

V. Sathish

{"title":"Principal Component Analysis of Morphological and Yield Attributing Traits in Advanced Breeding Lines of Rice Grown at Rainfed Shallow Lowland Condition of West Bengalvangaru","authors":"V. Sathish","doi":"10.24247/IJASROCT201732","DOIUrl":"https://doi.org/10.24247/IJASROCT201732","url":null,"abstract":"The current investigation was carried out used in the study comprising of high yielding advanced breeding lines collected from IVT-RSL, RRSS-Chakdah, Nadia, in a field experiment using a randomized block design with two replications to determine the relationship and genetic diversity among 49 rice germplasm accessions, using principal component analysis for rain fed shallow lowland condition. Observations were taken for Various morphological and yields and its attributing traits. In this study, Component-1 had the contribution from the traits viz., grain L/B ratio, grain length and harvest index which accounted 25.5 % of the total variance. Seed yield per plant, panicle weight, number of grains/panicle and number of florets/panicles have contributed 15.35 % of the total variability in component-2. No of secondary branches/panicle, days to 50% flowering and days to maturity contributed 12.97 % variability towards component-3. 1000-grain weight and grain breadth contributed 10.84% variation towards component-4. In principle component-5, characters plant height and panicle length together contributed 7.91% variability. The cumulative variance of 72.58 % of total variation among 20 characters was explained by the first five components. Thus the results of principal component analysis revealed wide genetic variability exists in this rice germplasm accessions.","PeriodicalId":79223,"journal":{"name":"International journal of biological research in pregnancy","volume":"1 1","pages":"265-270"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76804160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Proquinazid 20% EC on Powdery Mildew of Chilli Caused by Leveillula Taurica 20%丙喹酮乳酸菌对辣椒白粉病的防治效果评价

International journal of biological research in pregnancy Pub Date : 2017-01-01 DOI: 10.24247/IJBRDOCT20172

C. Ruth

引用次数: 0

Impact of various cooking methods on the micronutrient compositions of selected legumes in Eastern Nigeria 尼日利亚东部不同烹饪方法对选定豆类微量营养素组成的影响

International journal of biological research in pregnancy Pub Date : 2016-12-30 DOI: 10.14419/IJBR.V5I1.6711

Agomuo Emmanuel, A. Peter, Iheka Chidiebere, Duru Majesty

{"title":"Impact of various cooking methods on the micronutrient compositions of selected legumes in Eastern Nigeria","authors":"Agomuo Emmanuel, A. Peter, Iheka Chidiebere, Duru Majesty","doi":"10.14419/IJBR.V5I1.6711","DOIUrl":"https://doi.org/10.14419/IJBR.V5I1.6711","url":null,"abstract":"The impact of various methods of cooking on the concentration of some micronutrients in African Breadfruit ( Treculia africana ), Melon seeds ( Citrullus vulgaris ), Groundnut ( Arachis hypogea ), African oil bean ( Pentaclethra macrophylla ), Cashew nut ( Anacardium occidentale ) and Coconut ( Cocos nucifera ) samples were evaluated using standard methods. The results show that only the β-carotene and potassium contents of African Breadfruit were significantly affected by the various forms of heat processing applied. A significant (p<0.05) increase in the β-carotene content was observed for the melon seeds samples on application of heat by boiling (19223.61±4.00mg/100g) and microwave cooking (19028.59±62.12mg/100g), compared to the raw samples (12462.13±39.11mg/100g), which was equally observed for, groundnut, and cashew nut samples. Heat processing by boiling, pressure cooking, and microwave cooking significantly reduced the K content of the melon seeds, while heat processing by frying caused no significant effect. The Mg, Mn, Na, Zn, and Ca contents of the melon seeds were unaffected by the forms of heat applied. For the oil bean samples, the total carotenoid, K, Na, and Ca contents showed a significant decrease on application of the processing methods used in this study, while a similar result was obtained for the heat processed coconut samples, with an additional change in the Mg content, on application of heat. This study has shown that, Boiling, Frying, Pressure cooking and Microwave cooking affected the micronutrient composition of the selected legumes. Cooking by boiling showed the most retention of the micronutrients whereas the frying and microwave cooking methods showed more losses in micronutrient content.","PeriodicalId":79223,"journal":{"name":"International journal of biological research in pregnancy","volume":"58 1","pages":"10-14"},"PeriodicalIF":0.0,"publicationDate":"2016-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74700634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Evaluation of phytochemical and nutritional potential of Talinum triangulare (Jacq) leaf, stem and root on human health Talinum triangulare (Jacq)叶、茎、根对人体健康的植物化学和营养潜力评价

International journal of biological research in pregnancy Pub Date : 2016-12-09 DOI: 10.14419/IJBR.V5I1.6897

Chinyere V. Ilodibia, N. Igboabuchi

{"title":"Evaluation of phytochemical and nutritional potential of Talinum triangulare (Jacq) leaf, stem and root on human health","authors":"Chinyere V. Ilodibia, N. Igboabuchi","doi":"10.14419/IJBR.V5I1.6897","DOIUrl":"https://doi.org/10.14419/IJBR.V5I1.6897","url":null,"abstract":"Phytochemical and nutritional potentials of various parts (leaf, stem and root) of Talinum triangulare were evaluated using standard techniques. Analysis of Variance (ANOVA) was employed in data analysis. All the parts of the species were found to contain nutrient compositions in varying levels. Dry matter and carbohydrate were highest in the stem (91.69±0.12 and 68.66±0.01) respectively. Moisture, ash content and crude fibre were highest in the root (9.78±0.04, 17.53±0.11and 15.32±0.03) respectively while ether extract and crude protein were highest in the leaf (1.09±0.01 and 17.72±0.17) respectively. The results showed also varying quantities of the phytochemicals in the leaf, stem and root with some parts lacking some of the phytochemicals. The leaf contained the highest percentage of all the phytochemical investigated alkaloid, flavonoid, tannin, sterol, Terpenoid, phenol and saponin (0.47±0.01, 0.26±0.00, 0.36±0.07, 0.03±0.01, 0.42±0.02, 0.02±0.01and 0.68±0.05 respectively. The root lacked sterol and phenol while stem lacked only phenol. The leaf, stem and root lacked cyanogenic glycoside. The results have indicated that these parts of the Talinum triangulare investigated are very nutritious and possessed bioactive compounds that could be extracted for the manufacture of new drug, food supplements and also be used in ethnomedicine for the treatment of diseases.","PeriodicalId":79223,"journal":{"name":"International journal of biological research in pregnancy","volume":"1 1","pages":"6-9"},"PeriodicalIF":0.0,"publicationDate":"2016-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79385311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Virulence factors and beta-lactamase production among vancomycin-resistant Enterococcus faecalis isolated from clinical samples and hospital environment 从临床样本和医院环境中分离的耐万古霉素粪肠球菌的毒力因子和β -内酰胺酶产生

International journal of biological research in pregnancy Pub Date : 2016-12-09 DOI: 10.14419/IJBR.V5I1.6811

M. David, Kennedy Imonitie, R. T. Osuntoyinbo, A. Olawale

{"title":"Virulence factors and beta-lactamase production among vancomycin-resistant Enterococcus faecalis isolated from clinical samples and hospital environment","authors":"M. David, Kennedy Imonitie, R. T. Osuntoyinbo, A. Olawale","doi":"10.14419/IJBR.V5I1.6811","DOIUrl":"https://doi.org/10.14419/IJBR.V5I1.6811","url":null,"abstract":"Enterococcus faecalis , though opportunistic pathogen has emerged as one of the leading nosocomial pathogens and has been implicated in different human infections. The severity of the infections caused by this organism is largely due to its complex pathogenic process. The objective of this study was to determine the carriage of virulence factors and vanA gene among the strains of vancomycin-resistant E. faecalis isolated from hospitals. Standard methods were used for isolation, antibiotic susceptibility and detection of virulence factors in the isolates. A total of one hundred and twenty three (123) samples were screened out of which 69 (45.70 %) yielded E. faecalis . The highest percentage of the isolates was recovered from the environment followed by the clinical samples. Children surgical ward had the highest occurrence of the test organism followed by male surgical ward. All the isolates were resistant to both amoxycillin/clavulanic acid and ceftazidime, while 98.55%, 89.86% and 53.62% were resistant to ampicillin, cefuroxime and gentamicin respectively. Only twenty seven (39.13%) of the isolates were resistant to the vancomycin. Among the vancomycin-resistant isolates, haemolysin had the highest occurrence (60.29%) followed by caseinase (55.88%). A total of 16 (59.26%) were beta-lactamase positive while 8 (29.63%) out of the isolates (vancomycin-resistant) were non-biofilm former while vanA genes was detected in 9 (33.33%) of the isolates. This study gives an insight to antibiotic resistant pattern of circulating Enterococcus faecalis and also the isolate showed varying patterns of virulent factors.","PeriodicalId":79223,"journal":{"name":"International journal of biological research in pregnancy","volume":"97 2 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2016-12-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91333569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9