International journal of biological research in pregnancy最新文献_第2页

MicroRNAs: new biomarker for diagnosis, prognosis, therapy prediction and therapeutic tools for acute lymphoblastic leukemia MicroRNAs:急性淋巴细胞白血病的诊断、预后、治疗预测和治疗工具的新生物标志物

International journal of biological research in pregnancy Pub Date : 2019-01-01 DOI: 10.14419/ijbr.v7i1.29429

Eman A Gobran M. Sc, Ghada NasrM Nasr PhD, Mohamed Y Nasr PhD, Mahmoud I Nasr PhD

{"title":"MicroRNAs: new biomarker for diagnosis, prognosis, therapy prediction and therapeutic tools for acute lymphoblastic leukemia","authors":"Eman A Gobran M. Sc, Ghada NasrM Nasr PhD, Mohamed Y Nasr PhD, Mahmoud I Nasr PhD","doi":"10.14419/ijbr.v7i1.29429","DOIUrl":"https://doi.org/10.14419/ijbr.v7i1.29429","url":null,"abstract":"Back ground: MicroRNA (miRNA) was originally discovered in Caenorhabditis elegans by Victor Ambrose in 1993. It was predicted that miRNA account for 1-6% of the human genome and regulated at least 33% of protein-coding genes. Recently, 945 distinct miRNAs molecules have been identified within the human genome. It has been associated with the pathogenesis, progression and prognosis of different diseases, such as leukemia. MiR-181 family is one of those miRNA families, which generally expressed in 70 species and various human cancers.Objective: Assessment of mir-181a and LDH as promising co-biomarkers in order to provide additional information influence decisions about treatment sequentially to improve health outcomes of ALL in the Egyptian children.Patients and methods: This study was conducted on 100 children; 50 with ALL (38 males and 12 females) as ALL group. Other 50 healthy age and sex matched children were collected randomly as control group. Serum lactate dehydrogenase (LDH) and miRNA-181a were evaluated for all participants.Results: The results showed that there was a statistical significant difference between ALL and control groups regarding both molecular and biochemical indications of mir-181a and LDH were about almost five time as the control value, (P = 0.001). The ROC curve analysis revealed that the studied LDH and mir-181a markers were highly sensitive, highly specific and highly accurate test in the differentiation between the two studied ALL and control groups, with cutoff: ALL if mir-181a > 2.071, ALL if LDH > 0.307 respectively.Conclusion: MiRNA-181a and LDH can be used as co-biomarkers for ALL and might be beneficial in early diagnosis.","PeriodicalId":79223,"journal":{"name":"International journal of biological research in pregnancy","volume":"52 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91160690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The effect of coconut water and benzyl amino purine (BAP) addition to the growth of pineapple from Sipahutar North Sumatera in vitro condition 在离体条件下，添加椰子水和苄基氨基嘌呤(BAP)对北苏门答腊西柏虎塔凤梨生长的影响

International journal of biological research in pregnancy Pub Date : 2018-07-26 DOI: 10.14419/ijbr.v6i2.13697

Harifah Insani, F. Harahap, Diky Setya Diningrat

{"title":"The effect of coconut water and benzyl amino purine (BAP) addition to the growth of pineapple from Sipahutar North Sumatera in vitro condition","authors":"Harifah Insani, F. Harahap, Diky Setya Diningrat","doi":"10.14419/ijbr.v6i2.13697","DOIUrl":"https://doi.org/10.14419/ijbr.v6i2.13697","url":null,"abstract":"Background: Pineapple fruit used in this study was obtained from Sipahutar sub-district, North Tapanuli regency, North Sumatra which was a superior commodity fruit. Propagation of the Sipahutar pineapple plant was conducted vegetatively using bud stems and crown shoots, but the amount was limited to be planted in large areas. Therefore, propagation through tissue culture techniques could be used as an alternative solution.Objective: Explants source were taken from plantlets in vitro aged 3 months were grown on Murashige and Skoog (MS) basic medium with the addition of Indole Acetic Acid (IAA) 1 ppm.Methods: Sipahutar pineapple tissue culture work has been done in YAHDI Plant Tissue Culture Laboratory, Medan. The study design used a factorial completely randomized design. The first factor is coconut water with concentration of 0%, 10%, 20%, and 30%, second factor is Benzyl Amino Purine (BAP) with concentration 0 ppm, 2 ppm and 4 ppm, so there were 12 combination with 3 repetition.Result: The results showed that the addition of coconut water on MS base medium with IAA of 1 ppm gave a significant effect on the number of leaf yield and shoot height. The provision of BAP on basic MS medium with IAA of 1 ppm gave a significant effect on the number of shoots produced, the number of leaves produced, the length of the leaves, the width ofleaves and the length of shoots. The interaction of coconut water and BAP addition on MS base medium with IAA of 1 ppm gave significant effect on leaf length, leaf width and shoot length as well.Conclusions: MS + IAA basic media combined with coconut water + BAP could stimulate the growth of Sipahutar pineapple in vitro.","PeriodicalId":79223,"journal":{"name":"International journal of biological research in pregnancy","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78779506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effects of MYO-inositol and Indole Butyric Acid (IBA) on the Formation of Pineapples Root (Ananas comosus L.) from Sipahutar North Sumatera In Vitro myo -肌醇和吲哚丁酸(IBA)对北苏门答腊Sipahutar凤梨离体根形成的影响

International journal of biological research in pregnancy Pub Date : 2018-07-25 DOI: 10.14419/ijbr.v6i2.13699

Arisah Hasanah, F. Harahap, R. Silaban

{"title":"The Effects of MYO-inositol and Indole Butyric Acid (IBA) on the Formation of Pineapples Root (Ananas comosus L.) from Sipahutar North Sumatera In Vitro","authors":"Arisah Hasanah, F. Harahap, R. Silaban","doi":"10.14419/ijbr.v6i2.13699","DOIUrl":"https://doi.org/10.14419/ijbr.v6i2.13699","url":null,"abstract":"Background: Rooting of pineapple from Sipahutar in vitro is an alternative to obtain superior seeds in large quantities to meet the needs of farmers and consumers.Objective: The purpose of this study was to investigate the effect of Myo-inositol and IBA on pineapple rooting (Ananas comosus L.) from Sipahutar in vitro.Methods: The research method used a completely randomized factorial design with two factors: Factor I: Myo-inositol consisted of [4] treatment concentrations: M0 = 0 g/l, M0.02 = 0.02 g/l, M0.04 = 0.04 gr/l, M0.06 = 0.06 gr/l. Factor II: IBA growth regulators consisted of [3] treatment concentrations: IB0 = 0 ppm, IB1.5 = 1.5 ppm, IB3 = 3 ppm. The parameters observed were time of root emergence, number of roots, number of leaves, time of leaf emergence, number of tillers, and time of its emergence observed each week, while the height of tillers, shoots, and whole shoots, root length, leaf width and leaf length were observed 12 weeks after planting (WAP).Results: The result of the research showed that root time appeared on [3] WAP. The increasing number of leaves and timing of the puppiesin 1 WAP. Myo-inositol had significant effects on root counts in the 0.06 gr/l and root length on Myo-inositol treatment in the 0.04 g/l. IBA did not have a real effect on the entire observation parameters. The interactions of Myo-inositol and IBA had significant effects on root number, number of leaves, leaf length and tiller height.Conclusions: Myo-inositol and IBA had significant effects on root number, leaf number, leaf length and shoot height of pineapple’s explant from Sipahutar. ","PeriodicalId":79223,"journal":{"name":"International journal of biological research in pregnancy","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82465767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial and antidiabetic potentials of processed finger millet (Eleusine coracana) 加工谷子的抗菌和抗糖尿病作用

International journal of biological research in pregnancy Pub Date : 2018-06-05 DOI: 10.14419/ijbr.v6i1.10726

Japhet Erasmus Aisoni, M. Yusha’u, Olukayode Olugbenga Orole

{"title":"Antimicrobial and antidiabetic potentials of processed finger millet (Eleusine coracana)","authors":"Japhet Erasmus Aisoni, M. Yusha’u, Olukayode Olugbenga Orole","doi":"10.14419/ijbr.v6i1.10726","DOIUrl":"https://doi.org/10.14419/ijbr.v6i1.10726","url":null,"abstract":"Finger millet is a food crop with energy value, phytochemical constituents, and minerals, hence its use as whole flour for traditional food preparation among traditional people. The study was designed to determine the glycaemic index and blood glucose level in albino rats, and the antimicrobial potential of processed finger millets. Finger millets were processed by fermentation and roasting, and methanolic extracts obtained used to test antimicrobial activity. Albino rats (18) divided into three groups were fed fermented, roasted, and unprocessed millet (control), and glycaemic index and blood glucose level determined. Another 25 rat were treated into 5 groups of diabetic rats without treatment, diabetic rats treated daily with Metformin, diabetic rats fed 20% millet, diabetic rats fed 40% millet, and untreated group (control). At 100% concentration, roasted and fermented millet obtained inhibition of 15mm and 14mm against Salmonella typhi, while the control drug 25mg/ml streptomycin had 17mm inhibition. While values obtained for the glycemic indexes were close though significantly different from each other (roasted - 36.83±1.23; fermented - 38.73±1.87), roasted millet brought about the highest reduction in blood glucose level 90.9 and 65.1mg/dl at 60 and 120min respectively compared with fermented millet with 125.1 and 100.2mg/dl respectively at the same time. Animals administered finger millet in Groups 3 and 4-showed regeneration of the islet cells. The millet had the capacity to bring about healing of necrotic cells while also restoring and maintaining glucose levels in blood of subjects.","PeriodicalId":79223,"journal":{"name":"International journal of biological research in pregnancy","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79651801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibacterial Activity and Antioxidant Activity of Carica Papaya on Some Enteric Bacterial Isolates of Public Health Importance 木瓜对几种具有公共卫生意义的肠道细菌分离株的抗菌活性和抗氧化活性

International journal of biological research in pregnancy Pub Date : 2018-01-01 DOI: 10.24247/ijbrddec20183

R. Sumathi, Tjprc

引用次数: 0

The Comparison of Composition, Physico-Chemical Properties of Cow and Caprine Milk 牛奶和羊奶成分、理化性质的比较

International journal of biological research in pregnancy Pub Date : 2018-01-01 DOI: 10.24247/IJBRDDEC20181

H. Devarajanaik.HDevarajanaik., Tjprc

引用次数: 0

Storage Studies of Cow and Caprine Milk Dahi Incorporated With ∝s-Casein Bioactive Peptides 添加s-酪蛋白生物活性肽的牛、羊乳大蛋白的贮藏研究

International journal of biological research in pregnancy Pub Date : 2018-01-01 DOI: 10.24247/ijbrddec20182

H. Devarajanaik.HDevarajanaik., Tjprc

引用次数: 0

Determination of viability in foodborne bacteria with inter-calating dyes: ethidium monoazide (EMA) and propidium monoazide (PMA) 用单叠氮乙啶(EMA)和单叠氮丙啶(PMA)间层染料测定食源性细菌活力

International journal of biological research in pregnancy Pub Date : 2017-11-29 DOI: 10.14419/IJBR.V5I2.8561

A. E. Telli, Y. Doğruer

{"title":"Determination of viability in foodborne bacteria with inter-calating dyes: ethidium monoazide (EMA) and propidium monoazide (PMA)","authors":"A. E. Telli, Y. Doğruer","doi":"10.14419/IJBR.V5I2.8561","DOIUrl":"https://doi.org/10.14419/IJBR.V5I2.8561","url":null,"abstract":"The ability to distinguish between living and dead cells is considered to be very important for biological researches. It is an important problem that the technology used up to day does not allow the quantitative differentiation of specific cells in a mixed cell community. Determination of whether the microorganisms present in the foods are in a viable form is an important phenomenon in determining the disease-forming potential. It is a fact that DNA, which is found in cells that lose their viability, can maintain its activity for a long time. Discrimination of live-dead cell occurs when the intercalating dye is covalently bound to DNA that is cleaved in the dead cell where membrane integrity is impaired. The formation of the covalent bond is activated by photoactivation. Inter-collating dyes only affect dead cells that are damaged by cell wall or membrane integrity. Due to the covalent binding of the inter-collating dye, DNA amplification cannot occur in PCR and other molecular techniques based on PCR. Among the non-permeable stains, it is accepted that PI is the most commonly used. PMA is identical to PI and additionally contains azide groups. Azide groups allow PMA to cross-covalently bond with DNA in bright light. Another inter-collating dyes with an azide group is ethidium mono azide (EMA).The The PMA molecule provides a higher selectivity on discrimination of live-dead cells by virtue of its’ higher charge when compared to EMA. Many researchers have combined EMA and PMA with PCR, Real-time PCR and LAMP in order to differentiate the live population of bacterial, viral, fungal and parasitic food-borne pathogens because they are claimed to be more successful in complex samples than in fluorescence based techniques.","PeriodicalId":79223,"journal":{"name":"International journal of biological research in pregnancy","volume":"117 1","pages":"59-62"},"PeriodicalIF":0.0,"publicationDate":"2017-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83161112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Attenuation ofmicrobial-induceddeterioration of cellu-lose fibersby hornwort (ceratophyllumdemersuml.) methanolic extract 角草(ceratophyllumdemersuml.)甲醇提取物对微生物诱导的纤维素纤维退化的衰减

International journal of biological research in pregnancy Pub Date : 2017-11-26 DOI: 10.14419/IJBR.V5I2.8479

Ali Mohamedomar, Ayman Taha Salah, A. Mohamed, M. Sheded

引用次数: 2

Herd management and breeding practices of holla sheep owners in south wollo zone, Ethiopia 埃塞俄比亚南wollo地区holla羊的牧群管理和饲养实践

International journal of biological research in pregnancy Pub Date : 2017-10-11 DOI: 10.14419/IJBR.V5I2.8333

Adina Akale, T. Mengesha, A. Getu, S. Abegaz

{"title":"Herd management and breeding practices of holla sheep owners in south wollo zone, Ethiopia","authors":"Adina Akale, T. Mengesha, A. Getu, S. Abegaz","doi":"10.14419/IJBR.V5I2.8333","DOIUrl":"https://doi.org/10.14419/IJBR.V5I2.8333","url":null,"abstract":"This study was aimed to generate organized information on selective breeding objectives, trait's preferences and major constraints of the Holla sheep production system in Kalu and Worebabo districts of South Wollo Zone under small holders' management conditions. The study was performed based on the semi-structured questionnaire, focused group discussions. Secondary data source analysis and field observations were used to generate the required information. In addition, simple random sampling technique was used to select 180 sheep owners. Simple descriptive statistics, ranking trial and chip-square test was considered. The primary purpose of keeping sheep was asset development (income generation) followed by breeding purposes with the index value of 0.365 and 0.201, respectively. Body size/Appearance was the primary preference in both districts for breeding ram index value of 0.258 and 0.207 Kalu and Worebabu, respectively. In the study areas, culling inferior sheep, weaning, record keeping, castrating ram at right age and provision of regular vaccination before disease out breaks were poorly practiced. Age at first sexual maturity of male sheep was 7.3±2.17and 7.23±1.34 months while for females 7.9±1.87 and 5.8±1.16 months in Kalu and Worebabo district, respectively. Disease prevalence and genetic potential were the major production constraints in the entire studied districts. In order to minimize the failure of breed improvement programs it is important to involve farmers considering the existing breeding practices, management systems and trait preferences of the community and the multipurpose roles of targeted animals.","PeriodicalId":79223,"journal":{"name":"International journal of biological research in pregnancy","volume":"6 1","pages":"41-47"},"PeriodicalIF":0.0,"publicationDate":"2017-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80963298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0