M C Moreau, Y Duval-Iflah, M C Muller, P Raibaud, M Vial, J C Gabilan, N Daniel
{"title":"[Effect of orally administered bovine lactoferrin and bovine IgG on the establishment of Escherichia coli in the digestive tract of gnotobiotic mice and human newborn infants].","authors":"M C Moreau, Y Duval-Iflah, M C Muller, P Raibaud, M Vial, J C Gabilan, N Daniel","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The bacteriostatic effect of the association bovine lactoferrin (LF) and bovine IgG (IgG) was studied in vitro and in vivo against two Escherichia coli strains, S17 and EMO1, isolated from the faecal flora of mouse and man, respectively. These two strains were sensitive in vitro to the bacteriostatic effect of LF + IgG. A kinetic study of the in vivo establishment of E. coli S17 was followed in axenic mice associated with that strain. Seven hours after inoculation, no difference was observed in the faecal level of E. coli between control mice and mice fed the same diet supplemented with LF + IgG. An in vivo study was also carried out in human newborns receiving either maternized milk (Nursie) or the same milk supplemented with LF + IgG during the first 48 h of life. One group of babies was inoculated at birth with E. coli EMO1, while another was not. Between the ages of 1 and 5 days, the kinetics of establishment of the E. coli strains spontaneously found in the digestive tract of non-inoculated babies was not significantly different between the group which received milk supplemented with LF + IgG and that which did not. This result was confirmed in infants inoculated with E. coli EMO1. Likewise, the faecal levels of E. coli EMO1 were similar in the supplemented and non-supplemented babies, and, already from day 1, the population level was high and only slight individual variations between babies of the same group were observed. These findings show that the in vitro bacteriostatic effect of LF + IgG on the growth of E. coli strains is not found in vivo.</p>","PeriodicalId":7904,"journal":{"name":"Annales de microbiologie","volume":"134B 3","pages":"429-41"},"PeriodicalIF":0.0,"publicationDate":"1983-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17432076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Fusobacterium necrophorum isolated from a hepatic abscess and from mastitic udder secretions in a heifer.","authors":"T Shinjo","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Strains of Fusobacterium necrophorum were isolated from secretions of an udder affected with mastitis (4 strains), an adjacent unaffected udder (3 strains), a superficial inguinal lymph node (2 strains) and a liver abscess (4 strains). These 13 strains were examined bacteriologically together with three known strains: F. necrophorum VPI-2891 and VPI-6161, and Sphaerophorus necrophorus (F. necrophorum) N-167. All strains produced butyric acid from glucose and propionic acid from lactic acid, were positive for the indole reaction, failed to hydrolyse esculin, caused beta-haemolysis and showed sensitivity to penicillin (500 U/ml). They were divided roughly into two groups on the basis of differences in their properties as described below. Six strains belonged to one group and ten strains to the other: the former agglutinated chick red blood cells, formed colonies of type A of Fiévez, were long rods, showed turbid growth in liquid medium and were highly pathogenic for mice; the latter strains, consisting of short bacilli and forming colonies of type B, had no or weak haemagglutinin, showed, in liquid medium, sedimentary growth with a clear supernatant and were not fatal to mice. The 4 strains isolated from the hepatic abscess as well as the F. necrophorum strain VPI-2891 and the Sphaerophorus necrophorus strain N-167 were classified into biovar A. The 9 strains isolated from udder and lymph node, as well as the F. necrophorum strain VPI-6161, were classified into biovar B.</p>","PeriodicalId":7904,"journal":{"name":"Annales de microbiologie","volume":"134B 3","pages":"401-9"},"PeriodicalIF":0.0,"publicationDate":"1983-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17432073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Isolation of a large aerobic sporulated sheathed bacteria from soil by elective culture].","authors":"F Pichinoty","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Eleven endospore-forming strains of a probably unknown species of Bacillus were isolated from samples of pasteurized soil by aerobic enrichment culture at 32 degrees C in a minimal medium containing benzoate, p-hydroxybenzoate or cyclohexane carboxylate, as source of carbon and energy. They grew in the form of unbranched, flexible filaments 1.8 micrograms in diameter and sometimes more than 1,000 micrograms in length. The organism was not coenocytic because transverse partitions were visible. The endospores were oval (1.8 x 2.2 micrograms) and did not deform the filaments. A thin, translucid sheath was present. The 11 strains were prototrophic, used benzoate and acetate as sources of carbon and energy but did not grow in common peptone media. Only the isolation technique has been described here.</p>","PeriodicalId":7904,"journal":{"name":"Annales de microbiologie","volume":"134B 3","pages":"443-6"},"PeriodicalIF":0.0,"publicationDate":"1983-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17432079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
H L David, C Asselineau, M Daffé, M A Lanéelle, F Clément, V Lévy-Frébault
{"title":"Taxonomy of mycobacterial strains isolated from the tissues of leprosy patients.","authors":"H L David, C Asselineau, M Daffé, M A Lanéelle, F Clément, V Lévy-Frébault","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Thirty-six slowly growing mycobacteria isolated from the tissues of leprosy patients were studied using 40 characteristics as well as susceptibility to 27 distinct mycobacteriophages. The composition in mycolic acids of selected strains was also studied. According to the data, the strains formed 5 clusters. Some of the clusters were possibly as yet undescribed species; however, comparison of the data with the known properties of Mycobacterium leprae leads to the conclusion that none of the strains were identical to the leprosy bacillus.</p>","PeriodicalId":7904,"journal":{"name":"Annales de microbiologie","volume":"134B 3","pages":"367-77"},"PeriodicalIF":0.0,"publicationDate":"1983-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17431337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"In vitro susceptibility of Mycobacterium fortuitum and M. chelonei to sisomicin, gentamicin and tobramycin.","authors":"M J Casal, F C Rodriguez","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The in vitro action of sisomicin, gentamicin and tobramycin against 62 strains of Mycobacterium fortuitum and 20 strains of M. chelonei was investigated. At a drug concentration of 4 micrograms/ml, 31, 31 and 11%, respectively, of M. fortuitum strains and 90, 25 and 15%, respectively, of M. chelonei strains, were inhibited.</p>","PeriodicalId":7904,"journal":{"name":"Annales de microbiologie","volume":"134B 3","pages":"451-4"},"PeriodicalIF":0.0,"publicationDate":"1983-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17729971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[New antigenic factors, O (O23) and H (H26), of Serratia marcescens].","authors":"S Le Minor, F Benazet, L Martin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A new O factor (O23) found in a Serratia marcescens strain of formula O23:H19, and a new H factor (H26) found in 8 strains of formula O18:H26 are described.</p>","PeriodicalId":7904,"journal":{"name":"Annales de microbiologie","volume":"134B 3","pages":"447-9"},"PeriodicalIF":0.0,"publicationDate":"1983-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17266807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Growth and macromolecular synthesis of spheroplasts of Mycobacterium smegmatis ATCC 14468.","authors":"M V Murty, T A Venkitasubramanian","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The growth and synthesis of macromolecules in spheroplasts of Mycobacterium smegmatis ATCC 14468 were found to be in a balanced state. Spheroplasts were not lysed during preparation and subsequent incubation, and were not contaminated with whole cells. Limited growth of spheroplasts was not due to depletion of medium constituents. High molar concentrations of osmotic stabilizer did not inhibit balanced growth. These studies establish that spheroplasts are metabolically active and can be used as a tool in cell wall synthesis and in genetic studies.</p>","PeriodicalId":7904,"journal":{"name":"Annales de microbiologie","volume":"134B 3","pages":"359-65"},"PeriodicalIF":0.0,"publicationDate":"1983-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17431336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Fragmentation of the lactose-protease plasmid in lactose-negative derivatives of Streptococcus lactis and S. lactis ssp. diacetylactis].","authors":"P Ramos, M Novel, M Lemosquet, G Novel","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>DNA analysis showed the presence of plasmids in all 29 strains of mesophilic lactic streptococci. Their number ranged from 1 to 9 and their molecular weight extended from 1.8 to 45 megadaltons (Md). Comparison of the plasmid profiles from 9 strains of Streptococcus lactis, 3 strains of S. lactis ssp. diacetylactis and from their Lac-,Prt-variants was carried out. In most of the strains, a single plasmid of 27 to 45 Md carried both the lactose and protease (lac and prt) determinants. In Lac- variants, this composite plasmid may at times be lost; in most derivatives, this plasmid was fragmented into several smaller plasmids. Fragmentation resulted in the inactivation of lac genes and often of prt genes; sometimes prt genes remained intact in one of these new plasmids. Fragmentation of the plasmid may also take place without inactivation of these metabolic genes. Restriction analysis of the lactose-protease plasmid from 3 strains of S. lactis and from one Lac-variant confirmed the plasmid fragmentation phenomenon, and led to a proposal for physical mapping of the plasmid and preliminary localization of the lac-prt genes.</p>","PeriodicalId":7904,"journal":{"name":"Annales de microbiologie","volume":"134B 3","pages":"387-99"},"PeriodicalIF":0.0,"publicationDate":"1983-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17387148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"[Detection of glycosidases in Pseudomonas of the fluorescent group: relation between serotype and glycosidase activities in P. aeruginosa].","authors":"W Hansen, E Yourassowsky","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Fluorescent Pseudomonas species (P. aeruginosa, P. fluorescens, P. putida) were tested for the presence of glycosidase activities (alpha-D-glucosidase, beta-D-glucosidase, alpha-D-galactosidase, beta-D-galactosidase, beta-xylosidase, alpha-D-mannosidase, alpha-L-fucosidase, beta-L-fucosidase, beta-D-glucuronidase and N-acetyl-beta-D-glucosaminidase). Some of the investigated glycosidases were always absent, while N-acetyl-beta-D-glucosaminidase was constantly present in all strains; 3 glycosidase activities were observed in association or separately. Serotype O11 of P. aeruginosa was found to be homogeneous with respect to some of those enzymatic activities. Search for beta-D-galactosidase, alpha-D-glucosidase and beta-D-glucosidase may be of diagnostic value in epidemiologic studies of P. aeruginosa.</p>","PeriodicalId":7904,"journal":{"name":"Annales de microbiologie","volume":"134B 3","pages":"411-9"},"PeriodicalIF":0.0,"publicationDate":"1983-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17486155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Saccharomyces cerevisiae: heat and gluculase sensitivities of starved cells.","authors":"S Paris, J R Pringle","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Exponentially growing populations were abruptly shifted to media lacking a nitrogen source, a sulphur source or a phosphorus source. When proliferation ceased, cells were homogeneously arrested at the beginning of the cell cycle and were resistant to killing by exposure to 52 degrees C and to cell wall degrading enzymes. The results suggest that these two types of resistance represent a general response to nutrient limitation and are characteristic of resting cells.</p>","PeriodicalId":7904,"journal":{"name":"Annales de microbiologie","volume":"134B 3","pages":"379-85"},"PeriodicalIF":0.0,"publicationDate":"1983-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"17432072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}