{"title":"耻垢分枝杆菌ATCC 14468球质体的生长和大分子合成。","authors":"M V Murty, T A Venkitasubramanian","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>The growth and synthesis of macromolecules in spheroplasts of Mycobacterium smegmatis ATCC 14468 were found to be in a balanced state. Spheroplasts were not lysed during preparation and subsequent incubation, and were not contaminated with whole cells. Limited growth of spheroplasts was not due to depletion of medium constituents. High molar concentrations of osmotic stabilizer did not inhibit balanced growth. These studies establish that spheroplasts are metabolically active and can be used as a tool in cell wall synthesis and in genetic studies.</p>","PeriodicalId":7904,"journal":{"name":"Annales de microbiologie","volume":"134B 3","pages":"359-65"},"PeriodicalIF":0.0000,"publicationDate":"1983-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Growth and macromolecular synthesis of spheroplasts of Mycobacterium smegmatis ATCC 14468.\",\"authors\":\"M V Murty, T A Venkitasubramanian\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The growth and synthesis of macromolecules in spheroplasts of Mycobacterium smegmatis ATCC 14468 were found to be in a balanced state. Spheroplasts were not lysed during preparation and subsequent incubation, and were not contaminated with whole cells. Limited growth of spheroplasts was not due to depletion of medium constituents. High molar concentrations of osmotic stabilizer did not inhibit balanced growth. These studies establish that spheroplasts are metabolically active and can be used as a tool in cell wall synthesis and in genetic studies.</p>\",\"PeriodicalId\":7904,\"journal\":{\"name\":\"Annales de microbiologie\",\"volume\":\"134B 3\",\"pages\":\"359-65\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1983-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Annales de microbiologie\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Annales de microbiologie","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Growth and macromolecular synthesis of spheroplasts of Mycobacterium smegmatis ATCC 14468.
The growth and synthesis of macromolecules in spheroplasts of Mycobacterium smegmatis ATCC 14468 were found to be in a balanced state. Spheroplasts were not lysed during preparation and subsequent incubation, and were not contaminated with whole cells. Limited growth of spheroplasts was not due to depletion of medium constituents. High molar concentrations of osmotic stabilizer did not inhibit balanced growth. These studies establish that spheroplasts are metabolically active and can be used as a tool in cell wall synthesis and in genetic studies.
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