Andrology最新文献

{"title":"Fructose as a key biomarker for prognostication in idiopathic obstructive azoospermia associated with CFTR and ADGRG2 gene variants.","authors":"Zhongkun Liang, Tuerxunayi Yalikun, Xiaohui Ji, Jin Li, Ling Zhou, Jing Zhang, Jing Tian, Hui Chen, Ping Yuan","doi":"10.1111/andr.70014","DOIUrl":"https://doi.org/10.1111/andr.70014","url":null,"abstract":"<p><strong>Background: </strong>Isolated obstructive azoospermia (iOA) is classified as a type of obstructive azoospermia (OA) where cases remain \"unexplained\" even after excluding congenital absence of the vas deferens and external factors. This study aimed to determine if certain semen characteristics in iOA patients could indicate underlying genetic variants.</p><p><strong>Objectives: </strong>To investigate CFTR and ADGRG2 gene variants in patients with idiopathic obstructive azoospermia (iOA) and identify semen parameters associated with these variants to guide genetic testing.</p><p><strong>Materials and methods: </strong>Seventy-six iOA patients were divided into three groups based on variant status: Group I, patients with at least two CFTR variants or a hemizygous ADGRG2 variant; Group II, patients with one CFTR variant and no ADGRG2 variants; Group III, patients without any CFTR or ADGRG2 variants. Associations between genotypes and clinical parameters were analyzed using univariate and multivariate logistic regression to identify potential biomarkers.</p><p><strong>Result(s): </strong>Thirty-six of the 76 patients (47.37%) carried one or more CFTR or ADGRG2 variants. Twenty distinct variants, including three novel variants (c.1851A > T and c.2426C > T in CFTR, c.1105G > A in ADGRG2), were identified. The most common variant was c.1210-12T[5] (5T) (18.42%), followed by c.1666A > G (4.61%) and c.4056G > C (3.95%). Group I patients had significantly lower ejaculate volume, pH, and fructose levels compared with Groups II and III. The combined area under the curve (AUC) for fructose, age, pH, and volume was 0.979, with fructose alone achieving an AUC of 0.952. Diagnostic efficacy for variant detection was highest (88.1%) at a fructose cutoff of 0.591 µmol/ejaculate, with a sensitivity of 0.958, a specificity of 0.923, a positive predictive value (PPV) of 0.895 and a negative predictive value (NPV) of 0.877.</p><p><strong>Discussion and conclusion: </strong>Approximately 47% of iOA patients carry CFTR and ADGRG2 variants, characterized by significantly lower semen volume, fructose, and pH. Fructose was identified as an independent predictor of CFTR and ADGRG2 variants, highlighting its potential utility in guiding genetic testing and clinical decision-making in iOA patients.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143571513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Initial motility and vitality predict the semen quality after long-term cryostorage, even in patients with restricted ejaculate parameters.","authors":"Simone Bier, Daniela Hanke, Michael Zitzmann, Sabine Kliesch, Verena Nordhoff","doi":"10.1111/andr.70019","DOIUrl":"https://doi.org/10.1111/andr.70019","url":null,"abstract":"<p><strong>Background: </strong>Cryopreservation of human semen is the cornerstone for preserving male fertility before gonadotoxic therapy or in cases of high variability in semen parameters. This is particular crucial in cases of severe oligoasthenoteratozoospermia (OAT), where diminished sperm counts may compromise planned intracytoplasmic sperm injection (ICSI) procedures. Previous investigations in donor programs have shown long-term storage effects, such as decreased motility in cryopreserved semen samples. However, these studies were based on patients exhibiting normozoospermic semen samples. To date, there has been no comprehensive evaluation of the effect of long-term cryostorage on sperm samples from individuals with compromised semen parameters.</p><p><strong>Objectives: </strong>The aim of this study was to identify the effect of long-term cryostorage on semen parameters such as motility and vitality. Additionally, we sought to identify variables, which could aid in predicting motility and vitality following the freeze-thaw process.</p><p><strong>Patients and methods: </strong>Within our center, we have archived sperm samples from 6022 patients cryopreserved between 2001 and 2019. Among these, 293 patients donated their samples for subsequent research following depot termination. We examined semen concentration, motility, morphology, and vitality of spermatozoa thawed after varying storage durations, alongside baseline metrics documented at the time of cryopreservation. Samples were stratified into three cohorts based on storage duration: 2.5 to ≤5 years, > 5 to ≤14 years, and > 14 years.</p><p><strong>Results: </strong>Our analysis revealed no changes in motility (p = 0.44), vitality (p = 0.08), or morphology (p = 0.44) across the cohorts. Regression analysis demonstrated that initial motility and sperm concentration were significantly associated with post-cryostorage motility, whereas storage duration was not (p = 0.72). Similarly, there was no association between storage duration and post-thaw value 2 vitality (p = 0.64).</p><p><strong>Discussion: </strong>The initial semen analysis as well as the evaluation of a short-term frozen sample immediately after cryopreservation, appeared to be the most important markers for predicting post-thaw motility and vitality.</p><p><strong>Conclusion: </strong>Our results demonstrate the reliability of long-term cryostorage of human spermatozoa for fertility preservation, even in individuals with constrained semen quality at the time of cryopreservation.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dupless: Toward a patient-friendly approach for erectile dysfunction nature differentiation - a study of 291 penile duplex Doppler ultrasound assessments.","authors":"Hille J Torenvlied, Kim E de Jager, Loes I Segerink, Rob C M Pelger, Jack J H Beck","doi":"10.1111/andr.70017","DOIUrl":"https://doi.org/10.1111/andr.70017","url":null,"abstract":"<p><strong>Background: </strong>Erectile dysfunction (ED) is a condition commonly classified as either psychogenic or organic. Traditional age-based categorizations are considered overly simplistic, yet many clinicians continue to rely on initial evaluation-patient symptoms and history, physical examination, blood tests, and questionnaires-for diagnosis due to limited modern tools.</p><p><strong>Objectives: </strong>This study aims to evaluate the predictive value of patient characteristics in individuals with \"ED of indeterminate origin\" following initial evaluation. Identifying these variables could enhance early diagnosis and reduce reliance on invasive procedures.</p><p><strong>Materials and methods: </strong>A retrospective cohort study was conducted on patients who underwent penile duplex Doppler ultrasound between January 2018 and January 2024 due to \"ED of indeterminate origin\". Patient data, including demographics, lifestyle factors, and medical history, were collected and analyzed using unpaired t-tests, chi-squared tests, Fisher's exact tests, and multivariate logistic regression to assess their predictive value.</p><p><strong>Results: </strong>Among the 291 patients in the cohort, 165 (56.7%) were diagnosed with organic ED and 113 (38.8%) with psychogenic ED. Significant differences in age, history of diabetes mellitus, and drug use were noted. Logistic regression revealed multicollinearity among the variables and explained only 5.8% of the variance in ED etiology. Subgroup analysis revealed that diabetes mellitus predicts organic ED in patients aged 40 years and older, while psychopathology is linked to psychogenic ED. No significant predictors were identified for patients under 40 years.</p><p><strong>Discussion and conclusion: </strong>The findings of this \"Dupless\" study highlight the limitations of relying solely on initial evaluation to differentiate ED etiology, stressing the need for additional diagnostic tools. While some predictive factors were identified, they proved insufficient for clinical use. Thus, an urgent need exists for the development of modern, noninvasive diagnostic tools to enhance ED classification. Future research could explore machine learning models to uncover complex patterns not evident in traditional statistical methods.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143522500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling SMAD family member 6 as a novel biomarker for prognosis and immunotherapy response in testicular germ cell tumors.","authors":"Huawei Lin, Xiaowen Lin, Peisheng Huang, Xiaoxue Yu, Jianming Liu, Liangliang Huang, Yanni Wei, Jiahong Chen, Zhouda Cai, Le Zhang, Junhong Deng, Zhuoyuan Lin, Yu Zheng, Jianming Lu","doi":"10.1111/andr.70015","DOIUrl":"https://doi.org/10.1111/andr.70015","url":null,"abstract":"<p><strong>Background and objectives: </strong>Despite its rarity, testicular germ cell tumor (TGCT) is commonly diagnosed in young males aged 20-40. In recent years, the global prevalence of TGCT has gradually increased, with 12-30% of patients experiencing relapse and metastasis. However, there are currently no reliable biomarkers for accurately predicting the prognosis of TGCT patients. Therefore, identifying novel biomarkers for risk stratification in TGCT is an immediate priority.</p><p><strong>Materials and methods: </strong>Using TGCT samples from multiple centers, we identified a novel prognostic biomarker (SMAD family member 6 [SMAD6]) through differential expression analysis, Cox regression, and survival analysis. Immunohistochemistry (IHC) was then employed to evaluate SMAD6 expression levels in normal testicular tissues and TGCT samples. Finally, we examined the relationship between SMAD6 and its biological characteristics, mutation landscape, immune cell infiltration, and response to immunotherapy.</p><p><strong>Results: </strong>Our study identified SMAD6 as a risk factor for TGCT prognosis. IHC revealed significant expression of SMAD6 in TGCT tissues. Functional enrichment analysis indicated that SMAD6 may contribute to the activation of tumor progression-related pathways and suppression of immune-related pathways. Additionally, high SMAD6 expression was correlated with reduced CD8⁺ T cell infiltration, while patients with low SMAD6 expression benefited more from immunotherapy.</p><p><strong>Discussion and conclusions: </strong>This study highlights the potential of SMAD6 may be useful for TGCT prognosis and immunotherapy response prediction, offering a promising target for personalized medicine strategies.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143490520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Opioid-induced androgen deficiency in men: Prevalence, pathophysiology, and efficacy of testosterone therapy.","authors":"Hussein Kafel, Milena Braga-Basaria, Shehzad Basaria","doi":"10.1111/andr.70013","DOIUrl":"https://doi.org/10.1111/andr.70013","url":null,"abstract":"<p><strong>Background: </strong>Opioid analgesics are frequently prescribed for the treatment of chronic pain and are a common cause of male androgen deficiency. Despite its high prevalence, this adverse effect of chronic opioid use remains underappreciated by clinicians. As a result, androgen deficiency remains underdiagnosed and likely undertreated. This focused review discusses the expanding literature on opioid-induced androgen deficiency and the efficacy of testosterone therapy, with a particular focus on its anti-nociceptive effects.</p><p><strong>Methods: </strong>Original and review articles on opioid-induced male androgen deficiency published from 1950 through June 2024 were retrieved from PubMed using the key terms \"opioids,\" \"hypogonadism,\" \"low testosterone,\" and \"testosterone therapy.\" References within the retrieved publications were also researched.</p><p><strong>Results: </strong>Opioids suppress the gonadal axis mainly by inhibiting GnRH synthesis and secretion. The prevalence of opioid-induced androgen deficiency in men varies between 20% and 80% and is influenced by the type of opioid used, duration of exposure, age of the cohort, and how low testosterone was defined. Limited data from clinical trials suggest that testosterone therapy improves libido, body composition, and certain domains of quality of life. Early evidence also suggests that testosterone has anti-nociceptive properties, confirming findings from preclinical and population studies.</p><p><strong>Conclusion: </strong>Chronic opioid use is a common but underappreciated cause of androgen deficiency in men. There is a need to raise awareness among clinicians regarding this adverse effect of opioid use. Testosterone therapy could be considered in men with unequivocal androgen deficiency after a thorough clinical evaluation. Ongoing clinical trials will shed further light on the efficacy of testosterone therapy, particularly regarding its anti-nociceptive effects.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143466794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Loss of lncRNAs 1700101O22Rik and 1700027A15Rik causes sperm malformation and subfertility.","authors":"Shijue Dong, Ziyi Cai, Jingyan Yu, Min Liang, Yang Zhou, Mengqian Ding, Xuhui Zeng, Xiaoning Zhang","doi":"10.1111/andr.70011","DOIUrl":"https://doi.org/10.1111/andr.70011","url":null,"abstract":"<p><strong>Background: </strong>The testis is a key reservoir of long non-coding RNAs, yet their physiological roles in male reproduction remain debated. Notably, long non-coding RNAs 1700101O22Rik (22Rik) and 1700027A15Rik (15Rik) are uniquely expressed in the mouse testis. Previous research indicates that both 22Rik and 15Rik play roles in male reproductive processes; however, it is still unclear whether their effects on fertility are cumulative or compensatory.</p><p><strong>Objectives: </strong>To investigate the influence of simultaneous deletion of 22Rik and 15Rik on male reproduction and whether there are additive effects.</p><p><strong>Materials and methods: </strong>22Rik and 15Rik knockout mice were generated using CRISPR-Cas9, and double knockout mice were obtained through co-caging. To investigate reproductive phenotypes, we utilized computer-aided sperm analysis, acrosome reaction assessments, in vitro fertilization techniques, and sperm morphology analysis. Additionally, RNA sequencing and RNA binding protein immunoprecipitation were employed to explore the regulatory mechanisms of 22Rik and 15Rik.</p><p><strong>Results: </strong>The simultaneous deletion of 22Rik and 15Rik led to abnormal sperm morphology, impaired acrosome reaction, and reduced in vitro fertilization. Sperm count and fertility were also decreased in double knockout male mice. Compared to the knockout of long non-coding RNA 22Rik, reproductive abnormalities were somewhat exacerbated but largely similar to those observed with 15Rik knockout alone because of shared targeted genes, particularly Y chromosome-linked genes. Additionally, these abnormal phenotypes may be linked to reduced expression of transition protein 1 and dysfunction of the HSF2‒Rik22‒Rik15 complex in double knockout mice.</p><p><strong>Discussion and conclusion: </strong>Our study demonstrates for the first time that simultaneous knockout of these two long non-coding RNAs adversely affects sperm morphology and function by disrupting the HSF2‒Rik22‒Rik15 complex. Moreover, many overlapping regulated genes suggest that 22Rik and 15Rik may share similar regulatory mechanisms at the molecular level. This research sheds light on the causes and mechanisms behind sperm malformation and impaired male fertility.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143412986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First application of three-dimensional light sheet fluorescence microscopy to human testicular tumors: New perspectives in histopathology.","authors":"Diana Pinkert-Leetsch, Ahmad Kareem, Simon F Merz, Marc Teckentrup, Alexander Fichtner, Felix Bremmer, Jeannine Missbach-Guentner","doi":"10.1111/andr.70009","DOIUrl":"https://doi.org/10.1111/andr.70009","url":null,"abstract":"<p><strong>Background: </strong>Testicular tumors are among the most frequently diagnosed cancers in young men. The consequences of this diagnosis are orchiectomies, severely restricting fertility. For these young patients, a comprehensive diagnostics would be desirable, achieving a refined diagnosis and improved therapeutic patient stratification.</p><p><strong>Objective: </strong>The aim of this study was to use three-dimensional (3D) light sheet fluorescence microscopy (LSFM) to analyze a complete testicular tumor punch at subcellular resolution-allowing a detailed diagnostic assessment of the entire punch.</p><p><strong>Materials and methods: </strong>Tissue punches (3  and 5 mm diameter) were taken from paraffin blocks of four miscellaneous testicular tumors. After deparaffinization and clearing using benzoic acid/benzyl benzoate, a label-free LSFM autofluorescence imaging was performed. In addition, TO-PRO-3 nuclear stain was applied to several punches. After the scan, the samples were embedded in paraffin again and physically sectioned for conventional planar histology.</p><p><strong>Results: </strong>Based on the specific autofluorescence, not only the general morphology of the tumor tissue was identified in LSFM datasets, but also diagnostic features like infiltrations, papillary and pagetoid tumor cell formations, germ cell neoplasia in situ and azoospermia. Subcellular characteristics such as vacuolated cytoplasm and pleomorphic nuclei could be detected at maximum magnification. After nuclear staining, virtual H&E sections were reconstructed from the LSFM data and tomographically visualized across the entire punch. Subsequent histology and immunohistochemistry after LSFM analyses is possible.</p><p><strong>Discussion: </strong>LSFM analysis of testicular tumors enables the detailed 2D/3D analysis of an entire tumor punch for assessment of relevant tumor characteristics due to its intrinsic fluorescence or with specific nuclear staining.</p><p><strong>Conclusion: </strong>LSFM provides the technical basis for the analyses of complete testicular tumor biopsies, thus maximizing the spatial morphological and anatomical information. The subcellular 3D imaging of the tumor has the potential to identify new cancer imaging biomarkers that have additional diagnostic and prognostic value for patients.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143397982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive diagnostic and therapeutic approach to male factor infertility aimed at natural fertility: A multicentric retrospective cohort study.","authors":"Giuseppe Grande, Andrea Garolla, Andrea Graziani, Anna Laura Astorri, Maria Vittoria Cammarota, Annamaria Merola, Maria Pia Polidori, Emanuela Lulli, Enrico Busato, Francesco Pesce, Giuseppina Pompa, Alfredo Pontecorvi, Domenico Milardi, Alberto Ferlin","doi":"10.1111/andr.70006","DOIUrl":"https://doi.org/10.1111/andr.70006","url":null,"abstract":"<p><strong>Background: </strong>In infertile couples whose male partner has alterations in semen parameters frequently, a comprehensive andrological approach is lacking and approximately 30-50% are classified as idiopathic infertility. These couples are often directly addressed to assisted reproduction techniques (ARTs). However, several clinical conditions may benefit from medical treatment. By acting on etiology and/or risk factors, this aims at improving seminal parameters and restoring natural fertility.</p><p><strong>Objectives: </strong>To verify the impact of a comprehensive andrological assessment on the management of infertility (in particular, in couples with isolated male factor infertility) using as the primary outcome the natural pregnancy rate.</p><p><strong>Materials and methods: </strong>A multicenter retrospective study was conducted between 2015 and 2022 in 1014 couples with primary infertility seeking natural conception (including 266 couples with previous ART failure). Each couple underwent a multidisciplinary evaluation. This involved: a gynecologist and an andrologist both with expertise in infertility, a psychologist when requested, and a fertility awareness practitioner according to a unique diagnostic and therapeutic multidisciplinary protocol.</p><p><strong>Results: </strong>An isolated male factor was found in 23% of couples. In 45%, it was associated with female factors also. The comprehensive diagnostic approach reduced the proportion of idiopathic infertility to 8% of the couples. Targeted treatment, based on diagnostic categories, was associated with spontaneous pregnancy in 40.9% of the couples. In the 233 cases without female factors, normal semen parameters were observed only in 13% of patients. Male genital tract inflammation was observed in 48.8% of the patients, genital tract infection in 43.1%, and hypospermatogenesis in 16.7%. Patients with infections were treated with antibiotics and probiotics. If further inflammation was documented, this was followed by low-dose corticosteroids and antioxidants. Follicle stimulating hormone (FSH) treatment was used in patients with hypospermatogenesis, and varicocele repair surgery was performed in four patients.</p><p><strong>Discussion and conclusions: </strong>Our data underline the efficacy of a comprehensive approach to the diagnostic process of male factor infertility, both in reducing the percentage of idiopathic infertility and in restoring natural fertility based on a targeted treatment.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143389737","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The association of hypertension and antihypertensive medications on semen parameters among men presenting for fertility evaluation.","authors":"Daniel R Greenberg, Luis C Gago, Sai Kaushik S R Kumar, Evan J Panken, Kian Asanad, Zequn Sun, Robert E Brannigan, Joshua A Halpern","doi":"10.1111/andr.70010","DOIUrl":"https://doi.org/10.1111/andr.70010","url":null,"abstract":"<p><strong>Background: </strong>Hypertension is a common medical condition and its prevalence increases with age. Therefore, more prospective fathers will present for fertility evaluation with this diagnosis.</p><p><strong>Objective: </strong>To determine if hypertension and antihypertensive medication use are associated with impaired semen parameters.</p><p><strong>Methods: </strong>We retrospectively reviewed men with and without hypertension at the time of their index semen analysis (SA) between 2002 and 2023. Demographics, medical comorbidities, and semen parameters were evaluated between cohorts. Univariable and multivariable regression analyses were also used to determine the association of hypertension, and number and class of antihypertensive medications, and abnormal total motile sperm count (TMSC < 20 million).</p><p><strong>Results: </strong>Among 14,009 men, 10.1% (n = 1410) had a diagnosis of hypertension. Hypertensive men had significantly lower ejaculate volume (2.8 mL [interquartile range {IQR} 1.8-3.8] vs. 2.9 mL [IQR 2.0-4.0], p < 0.001) and sperm motility (58% [IQR 50-66] vs. 60% [52-68], p < 0.001). Hypertension was also independently associated with abnormal TMSC (odds ratio [OR] 1.21, 95% confidence interval [CI] 1.05-1.40, p = 0.008) on multivariable analysis. Among patients with hypertension, men with an active antihypertensive medication prescription at the time of index SA were older and had lower sperm motility (57% [IQR 47-64] vs. 59% [IQR 52-67], p = 0.006), sperm morphology (5% [IQR 2-7] vs. 6% [3-12], p < 0.001) and TMSC (48.9 M  [IQR 16.1-94.9] vs. 68.4 M  [25.0-124.9], p < 0.001) compared to patients with no prior antihypertensive medication exposure. Multivariable analysis demonstrated no significant increased risk of abnormal TMSC between unexposed patients and those taking an antihypertensive medication.</p><p><strong>Discussion: </strong>More than one in 10 men presenting for initial fertility evaluation had a diagnosis of hypertension. This diagnosis, as well as antihypertensive medication exposure, were associated with impaired semen parameters.</p><p><strong>Conclusion: </strong>Patients interested in future fertility should be counseled regarding lifestyle modifications to appropriately treat hypertension. Further studies are required to determine the impact of antihypertensive medications and adequate control of hypertension on semen quality.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143254242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The use of deidentified organ donor testes for research.","authors":"Marina V Pryzhkova, Marnie W Skinner, Juliana I Candelaria, Stephen R Wellard, Philip W Jordan","doi":"10.1111/andr.70008","DOIUrl":"https://doi.org/10.1111/andr.70008","url":null,"abstract":"<p><p>Our knowledge of testis development and function mainly comes from research using mammalian model organisms, primarily the mouse. However, there are integral differences between men and other mammalian species regarding cellular composition and expression profiles during fetal and post-natal testis development and in the mature testis. Therefore, to specifically learn more about human testis development and function, there is a need to use human testis tissue for research. Human testicular tissues that have been donated for research have allowed extensive molecular and cytological assessments, as well as single-cell transcriptome and epigenome analyses. These tissues have also been used for the development of cell technologies and in vitro models that aim to improve infertility treatments and diagnostics. Biopsied material taken from patients and designated for research is usually very small in size and is unsuitable for comprehensive studies. On the other hand, research using whole testes obtained from deceased, deidentified donors has become a valuable resource to assess conservation between humans and other organisms and identify human-specific phenomena. This review discusses the acquisition of donated deidentified human testes and their use for basic science research.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":""},"PeriodicalIF":3.2,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143254195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}