Andrology最新文献

{"title":"Comparative analysis of reproductive toxicity of polystyrene-nanoplastics and polystyrene-microplastics in rat Sertoli cells","authors":"Ying Hu,&nbsp;Shuyi Jiang,&nbsp;Ying Xu,&nbsp;Yuqi Zhang,&nbsp;Qiang Zhang,&nbsp;Wenjie Zhou,&nbsp;Jinhong Liang,&nbsp;Wenhui Su","doi":"10.1111/andr.70115","DOIUrl":"10.1111/andr.70115","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Microplastic pollution increasingly affects human health. Polystyrene nanoparticles (PS-NPs) and microplastics (PS-MPs) may impair Sertoli cells (SCs), vital for male fertility.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objectives</h3>\u0000 \u0000 <p>To compare PS-NPs (80 nm)/PS-MPs (8 µm) toxicity on rat SCs, focusing on oxidative stress, apoptosis, epithelial barrier integrity, endocytosis pathways, and miRNA-mediated ceRNA networks.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials/methods</h3>\u0000 \u0000 <p>SCs viability was assessed via CCK-8. Trans-epithelial electrical resistance (TER) was measured to assess the epithelial barrier function. Particle internalization was evaluated by confocal microscopy and flow cytometry. Endocytosis was detected by using specific inhibitors. Whole-transcriptome sequencing profiling identified differential expression of miRNAs, lncRNAs, circRNAs, and mRNAs, with subsequent ceRNA network construction. Pro-inflammatory factors and apoptosis were detected by RT-qPCR and flow cytometry, respectively.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>At 100 µg/mL, PS-NPs reduced cell viability to 77% versus 94% for PS-MPs (<i>p </i>&lt; 0.05) and decreased TER by 81% versus 63% for PS-MPs (<i>p </i>&lt; 0.01). PS-NPs were internalized via clathrin- and caveolin-dependent pathways, while PS-MPs remained extracellular. RNA-seq revealed PS-MPs activated inflammation pathways (Ank3/Daxx), while PS-NPs triggered oxidative stress and apoptosis pathways (Map2k4/Grin2a). PS-NPs induced higher apoptosis (17% vs. 9.3%, <i>p </i>&lt; 0.01), Reactive oxygen species (3.7-fold vs. 1.87-fold), and more severe catalase activity reduction (67% vs. 17%, <i>p </i>&lt; 0.01) compared to PS-MPs.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Discussion and conclusion</h3>\u0000 \u0000 <p>PS-NPs pose greater toxicity to SCs than PS-MPs due to cellular internalization, disrupting barrier integrity via oxidative stress/apoptosis. PS-MPs primarily trigger extracellular inflammation. Distinct ceRNA networks underpin their differential mechanisms. These results highlight risks of environmental microplastic fragmentation into nanoplastics, emphasizing the need for further research on microplastic impacts on male fertility.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":"14 7","pages":"2087-2104"},"PeriodicalIF":3.4,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144939514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Inaugural Pre-Treatment Nomogram to Assess the Eight-Week Efficacy of Dapoxetine: A Prospective Multi-Center Study","authors":"Guangdong Hou,&nbsp;Geng Zhang,&nbsp;Ming Gao,&nbsp;Yu Zheng,&nbsp;Elena Colonnello,&nbsp;Andrea Sansone,&nbsp;Lei Zhang,&nbsp;Tommaso B. Jannini,&nbsp;Ping Meng,&nbsp;Siyan Zhang,&nbsp;Emmanuele A. Jannini,&nbsp;Jianlin Yuan","doi":"10.1111/andr.70128","DOIUrl":"10.1111/andr.70128","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Although a nomogram for predicting the efficacy of dapoxetine (DapE-Nomo) has already been developed, its reliability is limited due to only 4 weeks of follow-up and a lack of external validation. Several patients with premature ejaculation (PE) achieve satisfactory therapeutic effects after longer periods of treatment clinically, we therefore aimed to develop and validate an 8-week DapE-Nomo.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The training cohort included 243 patients with lifelong PE from Xijing Hospital and Northwest Women's and Children's Hospital (Jan 2019–Jul 2020), while the validation cohort comprised 397 patients from Xijing Hospital and Xi'an Daxing Hospital (Aug 2020–Jan 2022). Efficacy was measured using the Clinical Global Impression of Change (CGIC) scale, with a CGIC score ≥ 1 indicating an improvement (iCGI). LASSO regression was utilized to identify the most valuable predictors (MVPs) of iCGI. The DapE-Nomo was developed utilizing logistic regression coefficients of MVPs and validated across both cohorts.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>After 8 weeks of medication, 47.7% of patients in the training cohort and 47.6% in the validation cohort achieved iCGI. MVPs of iCGI included intravaginal ejaculation latency time, difficulty delaying ejaculation, and education level. The DapE-Nomo showed discriminatory abilities of 0.722 and 0.709 in internal and external validations, respectively, with satisfactory calibration and clinical utility in both. The optimal cutoff value of the DapE-Nomo was identified as 153.4 in both cohorts. Individuals with scores ≥153.4 exhibited a 3.833-fold and 4.137-fold chance of achieving iCGI, respectively, compared with those with scores &lt; 153.4.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>We constructed and validated the inaugural 8-week DapE-Nomo. In outpatient settings, it will enable andrologists to more accurately evaluate the efficacy and promptly adjust treatment plans for patients with scores below 153.4. Moreover, It will help patients who've taken dapoxetine for 4 weeks with poor results decide whether to stop.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":"14 7","pages":"2126-2137"},"PeriodicalIF":3.4,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145249419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Analysis of In Vitro Aging in Liquid-Preserved Boar and Bull Semen Stored at 5°C and 16°C","authors":"Muhammad Umair Khan,&nbsp;Marie Luis Neubert,&nbsp;Sophie Henneberg,&nbsp;Markus Jung,&nbsp;Martin Schulze","doi":"10.1111/andr.70318","DOIUrl":"10.1111/andr.70318","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Although the prolonged liquid preservation of porcine and bovine semen is an attractive alternative to cryopreservation, the quality of the semen deteriorates over time due to in vitro aging.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>To investigate the in vitro aging of porcine and bovine sperm after prolonged liquid preservation at 5°C and 16°C.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Materials and Methods</h3>\u0000 \u0000 <p>Semen was collected from 10 Piétrain boars and 10 Holstein Friesian bulls. After dilution (boar: Androstar Premium; bull: Caprogen), semen samples (boar: 20 × 10⁶/mL; bull: 40 × 10⁶/mL sperm) were divided into two portions, which were stored at 5°C and 16°C for 6 days. Basic sperm quality parameters, such as progressive motility, thermo-resistance after 30 (TRT30) and 120 (TRT120) min at 38°C, and sperm morphology, as well as advanced multicolor assays, were examined on Days 1, 3, and 6 to evaluate viability, acrosome integrity, plasma membrane fluidity, intracellular calcium levels, and mitochondrial membrane potential. The data analysis was done using a general linear model (GLM) with repeated measures.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Compared to 5°C, boar semen showed significantly higher values for TRT120, viable and acrosome-intact sperm with low membrane fluidity and intracellular calcium (VAI-LMLC), and VAI with high mitochondrial membrane potential (VAI-HMMP) at 16°C. Bull semen demonstrated superior quality when stored at 5°C. The GLM revealed significantly higher values for TRT30, TRT120, morphology and VAI on Day 3 at 5°C compared to 16°C. Over the course of all storage days, the mean values of VAI-LMLC and VAI-HMMP were significantly higher at 5°C.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Discussion and Conclusion</h3>\u0000 \u0000 <p>Although basic semen quality could be maintained for 6 days in liquid-preserved boar and bull semen, advanced semen quality parameters revealed differences in the in vitro aging of boar and bull sperm that were specific to species and storage temperature.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":"14 7","pages":"2071-2080"},"PeriodicalIF":3.4,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148468502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Christina Chung-Lun Wang, MD (1944–2026): A Life Devoted to Andrology, Science, and People","authors":"Wei Yan","doi":"10.1111/andr.70346","DOIUrl":"https://doi.org/10.1111/andr.70346","url":null,"abstract":"&lt;p&gt;The andrology and reproductive medicine communities have lost one of their most respected and beloved leaders. Dr. Christina Chung-Lun Wang, an internationally renowned physician-scientist, mentor, colleague, and friend, passed away on June 10, 2026. Christina chose to keep her illness private, and I will honor that wish here. What I can say is that she continued to work, mentor, discuss data, attend meetings, and contribute to science until the very end. On June 6, only a few days before her passing, she was still presenting scientific data at a meeting.&lt;/p&gt;&lt;p&gt;For many of us, Christina helped define the fields of andrology, male reproductive endocrinology, male contraception, androgen biology, semen analysis, and reproductive medicine. For me, she was also a mentor, collaborator, advocate, neighbor, and dear friend. I have known many outstanding scientists, but very few who combined scientific judgment, clinical insight, efficiency, and personal generosity as naturally as Christina did.&lt;/p&gt;&lt;p&gt;Christina was born in Nanjing, China, on May 20, 1944. She received her medical degree from the University of Hong Kong, where she was awarded the John Anderson Gold Medal (proxime accessit) in 1968. She completed postgraduate training in medicine and endocrinology in Hong Kong and Australia and became a Fellow of the Royal Australasian College of Physicians in 1975. Before moving to the United States, she built a distinguished academic and clinical career in Hong Kong, eventually becoming Professor of Medicine and Chief of the Division of Endocrinology at Queen Mary Hospital. She moved to Los Angeles in 1989 to lead andrology services at Cedars-Sinai Medical Center and joined Harbor-UCLA Medical Center in 1993. Harbor-UCLA and The Lundquist Institute remained her academic home for more than three decades.&lt;/p&gt;&lt;p&gt;Christina's scientific contributions were broad, practical, and enduring. She played a major role in developing safe, effective, and reversible hormonal approaches to male contraception. She also made important contributions to androgen replacement therapy, male hypogonadism, spermatogenesis, reproductive aging, reproductive toxicology, and testicular biology. In addition, she helped establish technical and laboratory standards that have guided andrology laboratories around the world. Her work was never detached from human need; it consistently connected biological mechanisms with clinical relevance and patient care.&lt;/p&gt;&lt;p&gt;Her work with the World Health Organization (WHO) had an especially strong international impact. From 1991 to 2002, she chaired the WHO Task Force on Methods for the Regulation of Male Fertility and helped lead multicenter studies that shaped the global development of male contraceptive strategies. She also contributed to the WHO Laboratory Manual for the Examination of Human Semen from its first edition in 1980 through the sixth edition in 2021. With more than 350 peer-reviewed papers and over 70 chapters and reviews, Christin","PeriodicalId":7898,"journal":{"name":"Andrology","volume":"14 7","pages":"1903-1905"},"PeriodicalIF":3.4,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/andr.70346","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149011536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Density Gradient Centrifugation Versus Swim-Up for Cumulative Live Birth in IVF/ICSI Cycles With High Sperm DNA Fragmentation: A Propensity Score Matching Analysis.","authors":"Ziyu Luo, Xiangfeng Bai, Sufang Gui, Longda Wang, Wenjuan Yang, Ling Lu, Youlin Yao, Kai Wang, Shuhua Zhao","doi":"10.1111/andr.70377","DOIUrl":"https://doi.org/10.1111/andr.70377","url":null,"abstract":"<p><strong>Background: </strong>A high sperm DNA fragmentation index (DFI) has been found to be associated with male infertility and suboptimal outcomes in assisted reproductive technology (ART). Although sperm preparation techniques can reduce DFI following processing, it is unclear whether density gradient centrifugation (DGC) or swim-up (SU) achieves better clinical outcomes, particularly in terms of cumulative live birth rates (cLBR), for patients with elevated DFI.</p><p><strong>Objective: </strong>Using a propensity score-matched statistical approach, this study aims to compare the clinical effectiveness of DGC versus swim-up sperm preparation in male infertility patients with elevated sperm DFI. The primary objective is to determine which technique is associated with a higher cLBR following ART. Secondary objectives include evaluating post-processing DFI reduction, fertilization rates, embryo quality, high quality blastocyst rate, and other relevant laboratory and clinical outcomes.</p><p><strong>Materials and methods: </strong>This study was a retrospective design conducted at a single-center reproductive center. A total of 1769 couples were included in the study. They were receiving their first autologous IVF/ICSI cycle with a male partner who had a DFI of ≥ 20%. Following matching for propensity, 279 cycles were allocated to each of the DGC and SU groups. The primary outcome was cLBR per oocyte retrieval.</p><p><strong>Results: </strong>The SU group had a longer duration of abstinence and a higher proportion of conventional IVF cycles, while the DGC group included more ICSI cycles. There were no significant differences observed in cLBR (adjusted odds ratio [OR] = 1.06, 95% confidence interval [CI]: 0.76-1.49) or live birth rate per transfer (adjusted OR = 1.16, 95% CI: 0.89-1.52). The absolute difference in cLBR between groups was small. Laboratory outcomes, including fertilization rate, high-quality embryo rate, high-quality blastocyst rate, and blastocyst formation rate, showed similar point estimates, with no statistically significant differences. Results were broadly consistent across subgroups stratified by abstinence duration (less than 3 days, 3-5 days, or more than 5 days) and baseline semen parameter status (normal or abnormal).</p><p><strong>Discussion and conclusion: </strong>Among patients with DFI ≥ 20% undergoing IVF/ICSI, no statistically significant differences in cLBRs or laboratory outcomes were observed between DGC and SU. The effect estimates were close to the null and consistent across analyses; however, confidence intervals were wide, indicating limited precision. These findings suggest no clear evidence of a difference under the conditions studied. Further adequately powered prospective studies are warranted to more precisely estimate potential differences between these techniques.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149011811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Busulfan-Induced Male Infertility: Mechanisms, Therapeutic Interventions, and Future Directions","authors":"Tianyu Li,&nbsp;Zhichen Tang,&nbsp;Yaping Song,&nbsp;Xiaolei Luo,&nbsp;Bin Zhou,&nbsp;Xiao Liu,&nbsp;Fuping Li,&nbsp;Yanyun Wang,&nbsp;Lin Zhang","doi":"10.1111/andr.70189","DOIUrl":"10.1111/andr.70189","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <p>This review summarizes the molecular and cellular mechanisms underlying busulfan-induced male infertility and evaluates the translational potential of current and emerging therapeutic strategies. Evidence indicates that busulfan induces germ cell DNA alkylation damage, triggers sustained oxidative stress, and activates multiple cell death pathways. These events are accompanied by supporting cell dysfunction, disruption of the blood–testis barrier (BTB), and endocrine imbalance, collectively driving progressive impairment of spermatogenesis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <p>In response to these pathological processes, various interventions have been explored, including antioxidant molecules, natural extracts, epigenetic modulation, and stem cell–related approaches, many of which show partial improvement of spermatogenic function in experimental models. In addition, clinical strategies used for other forms of male infertility, such as gonadotropin supplementation and antioxidant supportive therapy, provide relevant reference frameworks for chemotherapy-associated fertility impairment. However, most studies on busulfan-induced infertility remain preclinical, and their benefits largely reflect supportive modulation of the testicular microenvironment rather than definitive restoration of spermatogonial stem cells (SSCs) or repair of genotoxic damage.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <p>Based on an integrated assessment of current evidence, this review highlights key unresolved challenges, including the lack of a defined therapeutic safety window, limitations in targeted delivery, and difficulties in achieving fundamental reconstruction of the spermatogenic system. Accordingly, future studies should place greater emphasis on more effective isolation and protection of testicular tissue, development of targeted drug delivery systems, and strategies aimed at spermatogenic system reconstruction.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":"14 7","pages":"1929-1940"},"PeriodicalIF":3.4,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/andr.70189","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146130983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Muscle Strength and Power Changes With Gender-Affirming Hormone Therapy","authors":"Mathilde Kamp Nørlund,&nbsp;Jakob Lindberg Nielsen,&nbsp;Marianne Skovsager Andersen,&nbsp;Rikke Hjortebjerg,&nbsp;Laust Frisenberg Buhl,&nbsp;Anne Pernille Hermann,&nbsp;Camilla Viola Buskbjerg Palm,&nbsp;Tine Taulbjerg Kristensen,&nbsp;Jan Frystyk,&nbsp;Per Aagaard,&nbsp;Jonas Mathiesen,&nbsp;Dorte Glintborg,&nbsp;Louise Lehmann Christensen","doi":"10.1111/andr.70132","DOIUrl":"10.1111/andr.70132","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Androgen receptors are highly expressed in upper body muscles, but changes in maximal muscle strength and power in major upper body muscles have not been investigated during gender-affirming hormone therapy (GAHT).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>To assess prospective changes in maximal muscle strength and muscle power from before and after 1 year of GAHT.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Design and Methods</h3>\u0000 \u0000 <p>Clinical prospective study in 92 transgender persons: 26 transmasculine treatment-naïve (TransM_naïve), 27 transmasculine treatment-ongoing (TransM_ongoing), 29 transfeminine treatment-naïve (TransF_naïve), and 10 transfeminine treatment-ongoing (TransF_ongoing) were examined at baseline and after 1 year of GAHT. Primary outcomes were unilateral upper body maximal muscle strength (kg) and mean and maximal power (watt) measured by low-row dynamic testing. Dual-energy X-ray absorptiometry was conducted in all participants.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>In transmasculine persons, the median (quartiles) age was 22 (20; 30) years in TransM_naïve and 26 (22–29) years in TransM_ongoing (median GAHT duration 3 (2; 5) years) at study inclusion. Maximal muscle strength increased 6.3 kg (12%) in TransM_naïve (<i>p</i> ≤ 0.001) and 5.0 kg (7%) in TransM_ongoing (<i>p</i> ≤ 0.001), while maximal muscle power remained unchanged. ∆-muscle strength was positively associated with ∆-lean mass in arms and negatively associated with age at GAHT initiation.</p>\u0000 \u0000 <p>In transfeminine persons, the median age was 24 (22; 30) years in TransF_naïve and 40 (31–64) years in TransF_ongoing (median GAHT duration 4 (2; 6) years) at study inclusion. Maximal muscle strength decreased 17.5 kg (23%) in TransF_naïve and -5.0 kg (8%) in TransF_ongoing, which was accompanied by a decrease (23%) in maximal muscle power in TransF_naïve.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Upper body muscle strength but not power increased during masculinizing GAHT, whereas muscle strength and power decreased within the first year of feminizing GAHT.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":"14 7","pages":"2138-2149"},"PeriodicalIF":3.4,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/andr.70132","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145273593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Live Birth Rates After Varicocele Embolization in Patients After Failed In Vitro Fertilization With Intracytoplasmic Sperm Injection","authors":"Anthony Verstandig,&nbsp;Adam Farkas","doi":"10.1111/andr.70328","DOIUrl":"10.1111/andr.70328","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background and Objectives</h3>\u0000 \u0000 <p>While in vitro fertilization with intracytoplasmic sperm injection (IVF-ICSI) bypasses abnormalities in sperm concentration, motility, and morphology, it still has a significant failure rate. We report our experience on the effect of varicocele embolization (VE) on couples with varicocele related primary male factor infertility in whom IVF/ICSI failed to result in a live birth (LB).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Material and Methods</h3>\u0000 \u0000 <p>This single center retrospective cohort study documents 42 couples who had failed to have a LB after single or multiple IVF/ICSI cycles and in whom the male partner subsequently had left or bilateral varicoceles treated by transcatheter embolization. Other inclusion criteria were maternal age of 35 or less at the time of VE and at least 24 months follow up after embolization. The end point was LB by natural conception or by IVF-ICSI.</p>\u0000 \u0000 <p>Pre-procedure data was documented at the time of referral for varicocele embolization. Images of the procedures were reviewed, and post VE data was collected by telephone questionnaire from one of the partners.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Prior to varicocele embolization, the 42 couples had undergone 151 oocyte retrievals (median 2, range 1–21) and 200 embryo transfers (median 3, range 1–26) with no LB.</p>\u0000 \u0000 <p>During a median follow up period of 54 months (range 25–111) following VE, there were 60 LB (65 children) amongst 36 of the 42 couples (86%). Thirty-four couples underwent a total of 119 oocyte retrievals and 149 embryo transfer procedures with 28 achieving 36 LB (41 children). Fifteen couples had 23 LB (23 children) from natural conceptions. One couple had an LB after intrauterine insemination.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>VE in this challenging patient population may treat varicocele associated sperm function problems not addressed by ICSI and could be beneficial before assisted reproductive techniques in varicocele related male factor infertility.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":"14 7","pages":"2081-2086"},"PeriodicalIF":3.4,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/andr.70328","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148719771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chromodomain Helicase DNA–Binding Proteins and Spermatogenesis: Current Advances","authors":"Mingrui Zhang,&nbsp;Di Liu,&nbsp;Guoqiang Liu,&nbsp;Minggang Zhu,&nbsp;Feng Pan","doi":"10.1111/andr.70232","DOIUrl":"10.1111/andr.70232","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Male infertility is a prevalent clinical condition, with approximately one-third of cases classified as idiopathic, frequently stemming from impaired spermatogenesis because of dysregulated gene expression. Chromodomain helicase DNA-binding (CHD) proteins are central chromatin remodelers that orchestrate this epigenetic regulation. For instance, smoking-induced hypermethylation of the <i>CHD5</i> gene promoter is associated with impaired semen parameters.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>This review aims to summarize the roles and mechanisms of CHD proteins in spermatogenesis, explore their relationship with male infertility, and discuss their potential as diagnostic and therapeutic targets.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>A comprehensive literature review was conducted to synthesize current findings on the expression and function of CHD proteins across different stages of spermatogenesis.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Research has shown that CHD proteins are expressed in the testis and play crucial roles in multiple stages of spermatogenesis, from spermatogonial stem cell (SSC) maintenance to spermatid maturation, making them promising targets for understanding and treating male infertility.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>CHD proteins represent important epigenetic regulators in spermatogenesis and may serve as valuable biomarkers or therapeutic targets for male infertility.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":"14 7","pages":"1962-1970"},"PeriodicalIF":3.4,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/andr.70232","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147643592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitochondrial Metabolism Governs Spermatogonial Stem Cell Fate Decisions","authors":"Zhimin Ma,&nbsp;Qi Geng,&nbsp;Huaibing Zhu,&nbsp;Dingfeng Zou,&nbsp;Dingyao Chen,&nbsp;Kai Li,&nbsp;Wei Song","doi":"10.1111/andr.70241","DOIUrl":"10.1111/andr.70241","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background</h3>\u0000 \u0000 <p>Spermatogonial stem cells (SSCs) sustain lifelong spermatogenesis through their unique capacity for self-renewal and differentiation. While transcriptomic regulation has long been emphasized, recent advances in multi-omics have highlighted mitochondrial metabolism as a pivotal regulator of SSC fate.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>This review aims to summarize current advances in how mitochondrial metabolism regulates SSC fate decisions and to explore its pathological alterations and potential therapeutic targets in obesity-associated male infertility.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>This review delves deeper into the core mechanisms by which mitochondria govern SSC fate decisions, integrating findings from multi-omics, metabolic assays, and animal models. We also provide a comprehensive overview of published literature on mitochondrial dysfunction in obesity-related infertility.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results and Conclusion</h3>\u0000 \u0000 <p>Quiescent SSCs predominantly rely on glycolysis within a hypoxic niche, whereas differentiation is accompanied by a metabolic shift toward oxidative phosphorylation (OXPHOS), alongside mitochondrial maturation and remodeling. Beyond energy production, mitochondria function as critical signaling hubs that integrate redox regulation, mitochondrial dynamics, and metabolite-dependent epigenetic mechanisms to dictate SSC fate. Conversely, in the context of obesity, mitochondrial dysfunction, characterized by excessive reactive oxygen species (ROS) production, mitochondrial fragmentation, and impaired quality control, severely disrupts SSC homeostasis and drives male infertility.</p>\u0000 \u0000 <p>Maintaining mitochondrial metabolic integrity is essential for normal SSC function. Targeting mitochondrial quality control and restoring metabolic balance represent promising emerging therapeutic strategies for the treatment of obesity-related male infertility.</p>\u0000 </section>\u0000 </div>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":"14 7","pages":"2018-2031"},"PeriodicalIF":3.4,"publicationDate":"2026-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/andr.70241","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148013210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}