Andrology最新文献

{"title":"Clinical Outcomes of Orchiopexy and the Risk of Malignancy in Postpubertal Cryptorchid Patients.","authors":"Xin Wu, Biao Dong, Xiaotao Li, Rui Chen, Chuanfu Ma, Jiaqing Liu, Yihui Shi, Xiaodong Wang, Zhuanxin Jiang","doi":"10.1111/andr.70278","DOIUrl":"10.1111/andr.70278","url":null,"abstract":"<p><strong>Background: </strong>Postpubertal cryptorchidism remains clinically challenging, as patients often present with long-standing maldescent, impaired spermatogenesis, and an increased risk of testicular malignancy. However, the optimal management of this population remains controversial, and comprehensive data on clinical characteristics, surgical outcomes, endocrine changes, and spermatogenic damage are still limited.</p><p><strong>Methods: </strong>We conducted a single-center retrospective cohort study of 194 patients (210 undescended testes) undergoing primary orchiopexy between January 2018 and June 2024. Clinical characteristics, testicular position, ultrasonographic volume, semen parameters, endocrine parameters, and histopathology findings were collected. Intraoperative biopsies were obtained in 200 testes (95.2%) and graded using the Johnsen score. Severe spermatogenic impairment was defined as a Johnsen score ≤3; analyses of spermatogenic impairment were performed in 164 patients with available histological and endocrine assessments. Surgical outcomes were assessed in 185 testes with ≥3-month follow-up, and oncologic events were assessed in 155 patients (161 testes) with available follow-up data. Univariable and multivariable logistic regression analyses were performed to identify predictors of severe spermatogenic impairment.</p><p><strong>Results: </strong>Most undescended testes were in a high position (196/210, 93.3%), and the affected testis had reduced volume in unilateral cases (median 6 vs. 18 mL). Overall anatomical success at 3 months was 80.0% (148/185), with recurrence and atrophy rates of 9.7% and 10.3%, respectively. Among patients with available semen data, unilateral cases showed no azoospermia, but 16 of 44 patients (36.4%) had at least one abnormality in sperm concentration, progressive motility, or normal morphology; by contrast, bilateral cases frequently presented with azoospermia or extremely low sperm counts. In unilateral cases, testosterone, LH, and FSH remained within the normal range without significant perioperative change; in bilateral cases, testosterone was generally preserved whereas FSH was elevated and remained above normal at follow-up. Histologically, only 17 of 200 biopsied testes (8.5%) had a Johnsen score >8, and 71/164 (43.3%) showed severe spermatogenic impairment. High testicular position was strongly associated with severe impairment (univariable OR 6.50, 95% CI 2.15-19.64, p < 0.001) and remained an independent predictor in multivariable analysis (adjusted OR 6.01, 95% CI 1.95-18.49, p = 0.002); affected testicular volume was independently inversely associated with severe impairment (adjusted OR 0.78 per 1 mL, 95% CI 0.64-0.95, p = 0.013). During a median follow-up of 54.6 months (range 12-78), one patient (0.5%) developed ipsilateral seminoma 48 months after orchiopexy.</p><p><strong>Conclusions: </strong>In postpubertal cryptorchidism, histological and functional spermatogenic impair","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":"1789-1800"},"PeriodicalIF":3.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13432487/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148238627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanisms of Polystyrene Microplastics in Multipathway Disruption of the Blood-Testis Barrier.","authors":"Haoran Liu, Adila Adili, Hui Tian, Xiaofang Han","doi":"10.1111/andr.70296","DOIUrl":"10.1111/andr.70296","url":null,"abstract":"<p><strong>Background: </strong>Polystyrene microplastics (PS-MPs) represent an emerging environmental pollutant, yet their potential threat to male reproductive health, specifically the integrity of the blood-testicular barrier (BTB), remains incompletely understood.</p><p><strong>Objective: </strong>This study aimed to investigate the toxicological effects and underlying mechanisms of PS-MPs exposure on testicular structure and BTB function in a mouse model.</p><p><strong>Methods: </strong>Four-week-old male ICR mice were exposed to PS-MPs at concentrations of 0, 500 µg/L, 5 mg/L, and 50 mg/L via drinking water for up to 5 weeks. Animals (n = 10 per group per time point) were sacrificed at weeks 1, 2, 3, 4, and 5 after initiation of exposure. A comprehensive assessment was performed, including evaluation of BTB permeability via biotin tracing, analysis of key junction protein (Connexin43, ZO-1, Occludin) expression and localization using western blot (WB), real-time quantitative polymerase chain reaction (RT-qPCR), and immunofluorescence, and immunofluorescent detection of macrophage activation (IBA-1).</p><p><strong>Results: </strong>PS-MPs exposure significantly increased BTB permeability in a dose- and time-dependent manner. This was accompanied by a marked downregulation in the expression and disrupted localization of Connexin43, ZO-1, and Occludin. We observed an upregulation of IBA-1 within the testis, indicating macrophage activation.</p><p><strong>Conclusion: </strong>This study demonstrates that PS-MPs induce BTB dysfunction in association with multipathway responses. Macrophage activation, as indicated by IBA-1 upregulation, was closely associated with BTB injury and may represent a key component in this toxicological process, although its precise mechanistic role requires further investigation. These findings provide new insights into the reproductive toxicity of microplastics.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":"1873-1881"},"PeriodicalIF":3.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13432557/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148306616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Online Andrology: Real-World Status Quo of Patients' Online Information Sources and Comparison With Large Language Models in Germany.","authors":"Traeger Max, Gloeckler Paulina, Hess Jochen, Kranz Jennifer, Morgenstern Saskia, Himmelsbach Ruth, Gratzke Christian, Schlager Daniel","doi":"10.1111/andr.70286","DOIUrl":"10.1111/andr.70286","url":null,"abstract":"<p><strong>Background: </strong>The internet, including websites and large language models (LLMs), is an increasingly important information resource for medical patients. However, information quality varies, potentially leading to misinformation. Andrological patients may particularly rely on online sources due to the sensitive nature of their conditions.</p><p><strong>Objectives: </strong>To assess the prevalence and quality of internet research among andrological patients and evaluate common online sources for comprehensibility, readability, and accuracy.</p><p><strong>Materials and methods: </strong>Patients (n = 283) at four German andrological centers completed a questionnaire on their online information behavior between November 2022 and October 2023. Common online sources were objectively evaluated using the DISCERN tool and Flesch readability index in 2023. Popular LLMs were also assessed in October 2024 and compared to traditional websites.</p><p><strong>Results: </strong>67% (n = 190/283) of andrological patients seek medical information before appointments, primarily using the internet (52%, n = 148/283) and general practitioners (49%, n = 139/283). Patients under 50 predominantly use online sources (60%, n = 74/122). Official medical association websites and Wikipedia are preferred, but 30% also use commercial sites (n = 91/283). In general, most common websites and LLMs provide sufficient information but lack easy comprehensibility and readability.</p><p><strong>Discussion: </strong>The study highlights the widespread use of online resources by andrological patients and emphasizes the importance of high-quality medical information. While online tools show promise, the value of physician-patient communication remains irreplaceable and cannot be replaced by chatting with LLMs.</p><p><strong>Conclusion: </strong>Official medical association should provide accurate and easily understood information for andrological patients. Efforts to improve the online media literacy and communication skills of medical personnel are necessary.</p><p><strong>Trial registration number: </strong>The study was registered in the German WHO primary registry, the German Clinical Trials Register (DRKS) (Number: DRKS00029651).</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":"1823-1830"},"PeriodicalIF":3.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13432683/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148300719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improvement of Mouse Spermatozoa Freezing at -80°C With Ascorbic Acid 2-Glucoside at Thawing Phase.","authors":"Alessia Paradiso, Renata Paoletti, Simone Cassetti, Nicola Bernabò, Barbara Barboni, Marcello Raspa, Ferdinando Scavizzi","doi":"10.1111/andr.70228","DOIUrl":"10.1111/andr.70228","url":null,"abstract":"<p><strong>Background: </strong>Sperm cryopreservation is the most common method to maintain a great number of mutant mouse lines. However, the use of liquid nitrogen (LN₂) for freezing presents considerable problems in terms of cost, safety, and accessibility. For this reason, the storage of semen samples at -80° in ultra-freezers has been increasingly used in recent years, with evident advantages: it does not require dedicated space, it is cheaper, and it is available even in small laboratories.</p><p><strong>Objectives: </strong>In this work, freezing at -80°C is tested in combination with antioxidant, aimed at reducing membrane damage and oxidative stress.</p><p><strong>Materials and methods: </strong>Wildtype mouse line sperm were cryopreserved using the CPM method directly at -80°C. The effect of ascorbic acid 2-O-alpha-glucoside (AA2G) spermatozoa treatment, during thawing, was evaluated through a qualitative (motility, vitality, and acrosomal reaction using SCA System) and functional analysis (IVF and embryo development), after 1, 6, and 12 months.</p><p><strong>Results: </strong>Sperm motility and velocity parameters showed no significant differences between treated and control groups across both B6N and B6J strains over 12 months, though overall motility declined with time. Antioxidant treatment (AA2G) significantly improved sperm viability and in vitro fertilization (IVF) outcomes in both strains, particularly B6N, without affecting acrosome reaction rates or blastocyst development.</p><p><strong>Discussion: </strong>Sperm cryopreservation at -80°C, while cost-effective, induces strain-dependent damage affecting sperm viability and fertility, particularly in B6J mice. Supplementation with the antioxidant AA2G during capacitation significantly improved post-thaw sperm survival and in vitro fertilization outcomes in both B6N and B6J strains, supporting its use in optimizing cryopreservation protocols without liquid nitrogen.</p><p><strong>Conclusion: </strong>These results may help to design new protocols or optimize those already validated by promoting the use of antioxidants in freezing at -80° as they have a significant effect on reducing oxidative stress, making spermatozoa qualitatively comparable to freezing in LN2.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":"1647-1655"},"PeriodicalIF":3.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13432668/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147669919","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Testicular SIRT1 Loss Reveals an Aging-Like Proteomic Landscape and Precipitates Reproductive Deterioration.","authors":"María Iniesta-Cuerda, Jiřina Havránková, Hedvika Řimnáčová, Jiří Moravec, František Liška, Martin Knytl, Milena Králíčková, Jan Nevoral","doi":"10.1111/andr.70201","DOIUrl":"10.1111/andr.70201","url":null,"abstract":"<p><strong>Background: </strong>Advanced paternal age is associated with reduced male fertility and testicular dysfunction. Among the molecular regulators involved in aging, SIRT1, a NAD⁺-dependent deacetylase, plays a pivotal role in maintaining oxidative balance and cellular homeostasis. Although the age-associated decline in SIRT1 levels is well established, the extent to which this reduction underlies testicular dysfunction and the specific proteomic alterations linked to it remain to be elucidated.</p><p><strong>Objective: </strong>This study aimed to determine whether testicular SIRT1 insufficiency contributes to testicular aging by promoting changes in the proteomic landscape and impairing male reproductive function.</p><p><strong>Materials and methods: </strong>We employed a Sirt1⁺/^- mouse model that mimics the partial SIRT1 decline observed during aging. Comparative analyses were conducted across wild type, aged wild type, and Sirt1⁺/^- males. We assessed reproductive performance, testicular histology, sperm quality, and embryo development. In parallel, we performed proteomic profiling of testicular tissue to identify molecular pathways affected by aging and SIRT1 insufficiency.</p><p><strong>Results: </strong>Sirt1⁺/^- males exhibited marked reproductive impairments, including reduced fertility, compromised embryo development, sperm morphological abnormalities, and increased testicular tubule degeneration. Proteomic analysis revealed substantial remodeling in both aged and Sirt1⁺/^- testes, with overlapping alterations affecting proteins involved in oxidative stress responses, proteostasis, and chromatin regulation. Moreover, several proteins with recognized anti-aging functions were undetectable in both aged and Sirt1⁺/^- models yet consistently expressed in wild-type testes. This deregulation reinforces the notion that testicular SIRT1 insufficiency recapitulates key features of the aging testis.</p><p><strong>Discussion: </strong>Our findings indicate that partial loss of SIRT1 is sufficient to trigger proteomic and functional hallmarks of testicular aging. In particular, we identified specific proteomic signatures linked to subfertility, including the loss of key capacitation-related proteins regulated by SIRT1 in the testis, a pattern also observed in aged animals, which may represent a mechanistic model of SIRT1-governed fertilization failure.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":"1579-1589"},"PeriodicalIF":3.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13432521/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147442196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomic Profiling of Cryopreservation-Induced Alterations in Rhesus Macaque Sperm.","authors":"Xiao-Jing Fan, Xiao-Lu Fan, Hai-Tao Zhang, Jun-Wen Zhang, Yun-Hui Liu, Xu-Dong Zhuang, Lu Chen, Xin-Rui Wang, Ya-Lan Wei","doi":"10.1111/andr.70303","DOIUrl":"10.1111/andr.70303","url":null,"abstract":"<p><strong>Background: </strong>Sperm cryopreservation is a fundamental technique in assisted reproductive technologies. While human sperm freezing is clinically routine, preserving nonhuman primate sperm remains challenging due to species-specific characteristics and low post-thaw viability.</p><p><strong>Objectives: </strong>This study aims to investigate the proteomic changes in rhesus macaque sperm before and after cryopreservation, focusing on identifying the molecular mechanisms behind cryoinjury.</p><p><strong>Materials and methods: </strong>Semen was collected from six sexually mature male rhesus macaques (6-10 years) using rectal electroejaculation. Motility parameters were quantified pre- and postcryopreservation using computer-assisted semen analysis (CASA) with vapor-phase liquid nitrogen protocols. Data-independent acquisition (DIA) quantitative proteomics, combined with LC-MS/MS, was used to profile global protein expression changes. Differential protein analysis was performed using multibioinformatics tools for functional annotation and network modeling. Papanicolaou and acridine orange (AO) staining were used to assess sperm morphology and DNA integrity, while reactive oxygen species (ROS) assay and immunofluorescence were employed to validate the proteomic analysis results.</p><p><strong>Results: </strong>We identified 512 differentially expressed proteins (32 upregulated, 480 downregulated), which are predominantly enriched in pathways related to metabolic reprogramming, oxidative stress response, and protein homeostasis. Immunofluorescence staining further confirmed markedly reduced levels of SOD1, PGK2, PKC‑alpha, and phospho‑tyrosine in sperm after freeze-thaw cycling. These molecular hubs are associated with sperm motility and functional stability and may be essential for regulating energy metabolism, oxidative balance, and proteotoxicity during cryopreservation-induced stress in rhesus macaque sperm.</p><p><strong>Conclusion: </strong>Cryopreservation induces profound proteomic remodeling in rhesus macaque sperm, mitochondrial electron transport, and metabolic homeostasis. Our findings reveal conserved and species-specific cryoinjury mechanisms, offering molecular targets for optimizing cryopreservation protocols in nonhuman primates to advance reproductive conservation strategies.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":"1882-1897"},"PeriodicalIF":3.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13432455/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148359039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Slit2/Robo Signaling Restores Diabetic Erectile Function via Neurovascular Remodeling.","authors":"Sen Fu, Peng Hu, Zizhong Yang, Zhenghui Jin, Xiaoyu Zhu, Tao Wang, Jihong Liu, Kai Cui","doi":"10.1111/andr.70288","DOIUrl":"10.1111/andr.70288","url":null,"abstract":"<p><strong>Background: </strong>Diabetes mellitus-induced erectile dysfunction (DMED) is a common and debilitating vascular-neurogenic complication of diabetes. The poor responsiveness of DMED patients to phosphodiesterase type-5 inhibitors underscores the need for therapies capable of restoring both endothelial integrity and cavernous nerve function. The Slit2/Roundabout (Robo) pathway, a conserved regulator of axon guidance and vascular remodeling, is well positioned to coordinate neurovascular repair; however, its involvement in DMED remains largely undefined.</p><p><strong>Methods: </strong>Single-cell RNA sequencing from embryonic genital tubercle to postnatal stages was used to map fibroblast-endothelial-neuronal signaling and identify Slit2 as a key secreted ligand. Diabetic and control mouse corpus cavernosum tissues were analyzed for endothelial and neuronal Slit2-Robo1/4 activity. In vitro, recombinant Slit2-N was applied to cells under high glucose to assess proliferation, migration, tube/sprout formation, and neurite outgrowth; Robo1/4 knockdown tested receptor dependency. In vivo, AAV-Slit2 was delivered intracavernously to diabetic mice, with erectile function assessed by sodium-nitroprusside perfusion and electrostimulation, and structural remodeling evaluated by endothelial, neural, and fibrosis markers.</p><p><strong>Results: </strong>Single-cell profiling identified Slit2 as a fibroblast-derived developmental cue for corpus cavernosum morphogenesis. Diabetic mice exhibited markedly reduced endothelial and neuronal Slit2-Robo signaling. Slit2-N restored angiogenic and neuroregenerative functions in vitro under high glucose, enhancing endothelial activity and neurite growth; all effects were lost after Robo1/4 silencing. In vivo, AAV-Slit2 re-established local Slit2 signaling, improved penile perfusion and erectile responses, enhanced endothelial proliferation and nerve regeneration, and reduced fibrosis, indicating substantial neurovascular recovery.</p><p><strong>Conclusions: </strong>Slit2/Robo signaling is a key regulatory axis disrupted in DMED. Local restoration of Slit2 via AAV delivery drives synchronized neurovascular regeneration and effectively rescues erectile function in diabetic mice. These findings establish Slit2 as a promising therapeutic target for reversing the underlying pathophysiology of DMED rather than merely providing symptomatic relief.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":"1831-1846"},"PeriodicalIF":3.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13432508/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148270137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the Pathways Linking Sleep Disturbance and Vasculogenic Erectile Dysfunction: The Dominant Role of Inflammation and Testosterone Mediation.","authors":"Xu Wu, Hui Gao, Guodong Liu, Hui Jiang, Emmanuele A Jannini, Yuyang Zhang, Xiansheng Zhang","doi":"10.1111/andr.70202","DOIUrl":"10.1111/andr.70202","url":null,"abstract":"<p><strong>Background: </strong>Poor sleep quality is associated with erectile dysfunction (ED), but the underlying mechanisms remain unclear. Given that systemic inflammation and testosterone levels are key factors in vascular health, this study investigates whether inflammatory biomarkers (high-sensitivity C-reactive protein [hs-CRP], interleukin-6 [IL-6], neutrophil-to-lymphocyte ratio [NLR], and platelet-to-lymphocyte ratio [PLR]) and total testosterone (TT) mediate the relationship between sleep quality and erectile function in vasculogenic ED patients.</p><p><strong>Methods: </strong>A total of 167 vasculogenic ED patients and 112 age-matched controls were recruited. Sleep quality was assessed using the Pittsburgh Sleep Quality Index (PSQI), and erectile function was evaluated using the International Index of Erectile Function-5 (IIEF-5). Inflammatory biomarkers and TT levels were measured, and principal component analysis (PCA) was applied to derive an inflammatory load score. Serial mediation analysis (PROCESS Model 6) was performed to examine whether inflammation and testosterone mediate the effect of sleep quality on erectile function, adjusting for potential confounders, including age, body mass index (BMI), smoking, triglycerides (TG), and total cholesterol (TC).</p><p><strong>Results: </strong>Patients with vasculogenic ED had poorer sleep quality (p < 0.001), lower TT levels (p < 0.01), and higher inflammatory biomarker levels (p < 0.01) than controls. Correlation analysis showed PSQI was positively correlated with inflammation (β = 0.371, p < 0.001) and negatively correlated with TT (β = -0.206, p < 0.01) and IIEF-5 (r = -0.437, p < 0.001). Mediation analysis revealed: Inflammatory biomarkers (hs-CRP, IL-6, NLR, and PLR) significantly mediated the association between sleep quality and ED, with hs-CRP showing the highest effect (β = -0.133, p < 0.001), accounting for 21.73% of the total effect. Testosterone also played a mediating role (β = -0.055, p < 0.01), contributing to 8.99% of the total effect. After combining inflammatory biomarkers into a PCA-derived score, the mediation effect of inflammation increased to 38.60%, whereas the testosterone pathway weakened.</p><p><strong>Conclusions: </strong>This study provides novel evidence that inflammation is a key mediator linking poor sleep quality to vasculogenic ED, while decreased testosterone levels play a secondary but still significant role.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":"1590-1599"},"PeriodicalIF":3.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13432624/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147300813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Testis-Enriched F-Box Protein FBXO39 Is Important for Spermiogenesis and Male Fertility in Mice.","authors":"Yuki Kaneda, Haruhiko Miyata, Masahito Ikawa","doi":"10.1111/andr.70225","DOIUrl":"10.1111/andr.70225","url":null,"abstract":"<p><strong>Background: </strong>The SCF (Skp-Cullin-F-box) complex is a major class of E3 ubiquitin ligases. F-box proteins constitute the SCF complex and play a critical role in recognizing substrates for ubiquitination. In mice, several F-box proteins, including FBXO36 and FBXO39, are predominantly expressed in testes.</p><p><strong>Objective: </strong>To investigate the roles of FBXO36 and FBXO39 in mouse male reproduction.</p><p><strong>Materials and methods: </strong>We generated Fbxo36 KO and Fbxo39 KO mouse lines using the CRISPR/Cas9 system. We evaluated male fertility, sections of reproductive organs, and sperm morphology and motility.</p><p><strong>Results: </strong>No overt abnormalities were found in male fertility or sperm morphology of Fbxo36 KO mice. In contrast, Fbxo39 KO males showed subfertility, abnormal sperm morphology, and reduced sperm motility. Histological analyses of testis sections revealed that FBXO39 is critical for the morphological transformation of round spermatids into spermatozoa.</p><p><strong>Discussion and conclusion: </strong>Our findings demonstrate that Fbxo39 is important for spermiogenesis in mice, whereas Fbxo36 is dispensable for male fertility. Further analyses of FBXO39 may contribute to understanding the etiology of male infertility.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":"1636-1646"},"PeriodicalIF":3.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13432620/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147589436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Different Sperm Isolation Strategies for Fresh and Cryopreserved Human Spermatozoa Using a Simple Method for Simultaneously Assessing Sperm Vitality and DNA Fragmentation.","authors":"R John Aitken, Sarah Lambourne, Alecia Sheridan, Hassan W Bakos","doi":"10.1111/andr.70282","DOIUrl":"10.1111/andr.70282","url":null,"abstract":"<p><strong>Background: </strong>This study compares three different methods of sperm isolation using a novel laboratory test that permits the parallel assessment of vitality and DNA integrity in the same sperm cell.</p><p><strong>Methods: </strong>Three different sperm isolation procedures were compared including the electrophoretic Felix System, swim-up and density gradient centrifugation (DGC), each of which utilized a different separation principle comprising net charge, intrinsic motility and isopycnic density, respectively. These sperm isolation methods were also challenged with the more difficult task of isolating spermatozoa following cryopreservation, which compromises sperm vitality, motility and DNA integrity. To facilitate this comparison, a novel assessment technique was developed wherein a live/dead stain that formed covalent bonds with intracellular amines in non-viable spermatozoa was combined with the Sperm Chromatin Dispersal (SCD) assay for DNA integrity.</p><p><strong>Results: </strong>Overall, Felix generated populations of spermatozoa that were superior to DGC (p < 0.001), and equivalent to swim-up, in terms of optimizing the proportion of 'live/DNA intact' cells and rejecting the 'dead/DNA intact' and 'dead/DNA damaged' subpopulations.</p><p><strong>Discussion and conclusions: </strong>The development of a novel method for the parallel assessment of vitality and DNA damage facilitated comparison of different sperm isolation techniques and may find applications in other areas of diagnostic andrology.</p>","PeriodicalId":7898,"journal":{"name":"Andrology","volume":" ","pages":"1809-1822"},"PeriodicalIF":3.4,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13432488/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148270178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}