Animal Gene最新文献

{"title":"A genome-wide search of meat productivity candidate genes in Russian Meat Merino breed","authors":"Alexander Krivoruchko ,&nbsp;Alexander Surov ,&nbsp;Anastasiya Kanibolotskaya ,&nbsp;Peter Sheludko ,&nbsp;Natalia Likhovid ,&nbsp;Olesya Yatsyk ,&nbsp;Andrey Likhovid","doi":"10.1016/j.angen.2023.200146","DOIUrl":"10.1016/j.angen.2023.200146","url":null,"abstract":"<div><p><span><span>A genome-wide associations study (GWAS) of single nucleotide polymorphisms (SNP) with indicators of meat productivity in Russian Meat </span>Merino was carried out. A new approach is based on the selection of animals for research considered with known productivity genes (</span><em>MSTN</em>, <em>MYOD1</em>, etc.). Animal genotyping was performed using Ovine Infinium HD BeadChip 600 K (Illumina, USA). As a result, 20 SNPs significantly associated with the body measurements and ultrasound parameters of the animals were identified. Around these SNPs, 14 candidate genes were discovered. Investigated parameters of phenotype can be associated with structural disorders in these genes. Most SNPs were located in the intergenic space, two polymorphisms were located in the Upstream and Downstream regions. One SNP was localized in the intron, two are in the exon of genes. Two substitutions were next to the long non-coding RNA and micro-RNA. The following candidate genes were annotated: <span><em>ASB3, THADA, PRPS1L1, Ccdc130, MTHFS, RALGAPA1, </em><em>MEIS1</em><em>, AKIRIN1, GRXCR1, Lrig1, ANKS1B, CFI, KRAB,</em></span> and <span><em>SLCO2B1</em></span><span><span>. These genes are involved in some metabolic processes of cell proliferation and differentiation, function of neurons and immune system. Further research should be oriented to study the structure of these genes and use discovered SNPs as new </span>molecular markers in breeding.</span></p></div>","PeriodicalId":7893,"journal":{"name":"Animal Gene","volume":"27 ","pages":"Article 200146"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48569461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Revisit of the taxonomic status of Bos genus with special refference to north eastern hilly region of India","authors":"Ningthoujam Neelima Devi ,&nbsp;Bishal Dhar ,&nbsp;Prasanta Kumar Bera ,&nbsp;Yashmin Choudhury ,&nbsp;Sankar Kumar Ghosh","doi":"10.1016/j.angen.2022.200143","DOIUrl":"10.1016/j.angen.2022.200143","url":null,"abstract":"<div><p><span>North-eastern India has diverse Bos genus endemic animals which include Siri (</span><span><em>Bos indicus</em></span>), Yak (<em>Bos grunniens</em><span>) and Mithun (</span><em>Bos frontalis</em><span>). But very little is known about their origin and taxonomic position. Hence, a taxonomic revisit of the genesis needed to confirm their position in the family Bovidae<span><span>. So, the present study focuses on molecular cataloguing through DNA barcoding and </span>phylogenetic relationships. The generated </span></span><em>CO1</em> sequences of <em>Bos indicus, Bos grunniens, and Bos frontalis</em> were analyzed through similarity search, Species delineation approach like K2P Distance, Neighbour-Joining tree and ML approaches to identify their taxonomic position with respect to global published data. The NJ tree revealed cohesive clustering of <em>Bos indicus</em> with the pre-existing sequences of zebu and taurine but distinct from other congeners. The collected specimen of <em>Bos frontalis</em> clustered distinctly from other congeners and failed to cluster with <span><em>Bos gaurus</em><em>,</em></span> which nullifies the previous report of <em>Bos frontalis</em> as a domesticated form of <em>Bos gaurus</em>. Similarly, <span><em>Bison bison</em></span> clustered very closely with <em>Bos grunniens</em> as their nearest neighbour. This observation was confirmed by ML phylogeny. Our findings confirmed the Siri's species status as <em>Bos indicus</em>. Similarly, <em>Bos frontalis</em> is a different species within the genus <em>Bos</em> but not the domesticated form of <em>Bos gaurus</em>. The close clustering of <em>Bos grunniens</em> and <em>Bison bison</em> supported the previous reports, which suggest that the genus <em>Bison</em> should be regarded as a synonym of the genus <em>Bos</em>. Thus, the present study strongly recommends the revisiting of bovine taxonomy.</p></div>","PeriodicalId":7893,"journal":{"name":"Animal Gene","volume":"27 ","pages":"Article 200143"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46681349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single Nucleotide Polymorphisms in Insulin-like Growth Factor 2 (IGF2) gene and their associations with body weight and growth rate traits in indigenous guinea fowls (Numida meleagris) of northern Ghana","authors":"Kurukulasuriya Mariesta Jayaroshini Ahiagbe ,&nbsp;Christopher Adenyo ,&nbsp;Miho Inoue-Murayama ,&nbsp;Esinam Nancy Amuzu-Aweh ,&nbsp;Patrick Bonney ,&nbsp;Boniface Baboreka Kayang","doi":"10.1016/j.angen.2022.200139","DOIUrl":"10.1016/j.angen.2022.200139","url":null,"abstract":"<div><p><span>Insulin-like Growth Factor 2 (IGF2) plays important roles in stimulating cell proliferation, differentiation and migration culminating its effects as a modulator of juvenile growth in animals. Although the gene that codes for IGF2 (</span><em>IGF2</em><span>) has been investigated as a candidate gene in several livestock species, there is no information on polymorphisms in </span><em>IGF2</em> in guinea fowls (<em>gIGF2</em><span>), an important indigenous poultry species from West Africa. Therefore, this study sought to identify the Single Nucleotide Polymorphisms (SNPs) in three populations of indigenous guinea fowls in northern Ghana. Target genomic regions in </span><em>gIGF2</em> were amplified and sequenced from 84 indigenous guinea fowls from Upper East Region (<em>n</em> = 17), former Northern Region (<em>n</em> = 22) and Upper West Region (<em>n</em> = 45) of Ghana together with domesticated French variety (<em>n</em><span> = 3). The sequences were aligned with the reference genomic sequence (domesticated French variety) of chromosome 6 (GenBank accession no., </span><span>NC_034414.1</span><svg><path></path></svg><span>) to identify SNPs. Statistical associations among the genotypes arising from the SNPs and juvenile growth traits were estimated using linear models. Two novel SNPs were identified in </span><em>gIGF2</em><span><span> among the indigenous guinea fowls. An insertion of a Guanine (G) within a poly G motif of the intron following the third exon at the 13,955,730 bp location was identified in the majority (84.5%) of indigenous guinea fowls, while the wild type allele was observed in the minority of indigenous guinea fowls and in the domesticated French variety sampled. Also, a biallelic transition arising from the substitution of G by </span>Adenine (A) at position 13,956,496 bp (13,956,496 G &gt; A) located on the fourth exon, which codes for most of the extension peptide of prepro IGF2, was observed in the minority (11.9%) of indigenous guinea fowls. No significant associations among the genotypes arising from the two SNPs, with body weights and weekly growth rates, were identified.</span></p></div>","PeriodicalId":7893,"journal":{"name":"Animal Gene","volume":"27 ","pages":"Article 200139"},"PeriodicalIF":0.0,"publicationDate":"2023-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46620782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Most reactions leading to neuropathic pain at dorsal root ganglion of rats with spinal nerve ligation have occurred in the early phase","authors":"Chunmei Wang,&nbsp;Xiaofang Lin,&nbsp;Wei Tang,&nbsp;Dongmei Wang","doi":"10.1016/j.angen.2022.200138","DOIUrl":"10.1016/j.angen.2022.200138","url":null,"abstract":"<div><p><span>In order to study the mechanism of early and late neuropathic pain, Ribonucleic acid<span><span> sequencing (RNA-seq) technique was used to analyze the dorsal root ganglion (DRG) </span>transcriptome<span> of rat induced by spinal nerve ligation (SNL). It was found that most of the reactions leading to neuropathic pain at dorsal root ganglion, such as pro-inflammatory response and immunity, occurred in the early phase.378 genes are up-regulated and 42 genes are down-regulated in the early phase, while in the late phase, there are 27 up-regulated genes and 23 down-regulated genes. Four new pain related genes are found: </span></span></span><em>Cd8a, Bub1b, Ccna2</em> and <em>Cdk1</em><span>. From the analysis of Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways and Gene Ontology<span> (GO) functions enriched by differentially expressed genes (DEGs), dorsal root ganglion should carry out immunities, pro-inflammations and related compensatory regulation in response to peripheral nerve injury in the early phase. The late phase is characterized by maintaining long-term or chronic inflammation.</span></span></p></div>","PeriodicalId":7893,"journal":{"name":"Animal Gene","volume":"26 ","pages":"Article 200138"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42555601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and characterization of new putative antimicrobial peptides from scorpion Chaerilus tricostatus revealed by in silico analysis and structure modeling","authors":"Marzieh Rostaminejad ,&nbsp;Amir Savardashtaki ,&nbsp;Mojtaba Mortazavi ,&nbsp;Sahar Khajeh","doi":"10.1016/j.angen.2022.200137","DOIUrl":"10.1016/j.angen.2022.200137","url":null,"abstract":"<div><p><span><span>Antimicrobial peptides (AMPs) are considered a promising therapeutic strategy because of their high potential for fighting against antibiotic-resistant pathogens. Scorpion venom is a rich resource of AMPs because of their biodiversity. Accordingly, we aimed to employ a bioinformatics-based approach to search for new putative AMPs from available omics datasets. The </span>amino acid sequence<span> of the peptide Ctriporin was used as query for a blast search in the UniProt and NCBI<span> databases, resulting in the identification of 14 homologous peptides from scorpion venom. To predict antimicrobial activity, all sequences were analyzed using various machine learning-based algorithms on the Collection of Anti-Microbial Peptides (CAMP</span></span></span>_R3) database. Furthermore, the online tools Antifp, AVPpred, and iACP were used to predict the antifungal, antiviral, and anticancer activity, respectively. The physicochemical properties were also evaluated by online tools and compared with Antimicrobial Peptide Database Calculator and Predictor (APD3). Finally, the three-dimensional structure modeling of the peptides Ctriporin, Ctri10036, Ctri9610, and Ctri10033 was performed using I-TASSER and PEP-FOLD 3.5. Our in silico analysis led to the identification of three new peptides with potential antimicrobial properties from the venom of the scorpion <em>Chaerilus tricostatus</em>, which can be suitable candidates for further experimental validation studies.</p></div>","PeriodicalId":7893,"journal":{"name":"Animal Gene","volume":"26 ","pages":"Article 200137"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43667557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential probiotic properties and molecular identification of lactic acid Bacteria isolated from fermented millet porridge or ragi koozh and jalebi batter","authors":"Manoharan Manovina ,&nbsp;Balasubramanian Thamarai Selvi ,&nbsp;Ragothaman Prathiviraj ,&nbsp;Joseph Selvin","doi":"10.1016/j.angen.2022.200134","DOIUrl":"10.1016/j.angen.2022.200134","url":null,"abstract":"<div><p><span><span>The consumer market has significantly increased in the search for nutritional and probiotic formulated products. In the present study, probiotic characterization parameters have been evaluated for the bacteria isolated from fermented </span>millet<span> porridge or ragi koozh and jalebi batter. Initially, 30 colonies were randomly picked and screened for lactic acid production. Lactic acid-producing 13 isolates showing gram-positive cell wall, non-spore-forming capacity and catalase-negative strains were subjected to probiotic characterization. Among 13 isolates, 2 isolates S9 and S13 exhibited good tolerance towards in-vitro characterization parameters such as acid and bile tolerance, adhesion to hydrocarbons, autoaggregation, co-aggregation, antibacterial activity, and safety measures. The potential probiotic strains S9 and S13 were identified by molecular methods, and evolutionary imprints and submitted to GenBank as </span></span><em>Weisella cibaria</em> and <span><em>Enterococcus</em><em> lactis</em></span>. Results showed the efficacy of probiotic characteristics of isolates S9 and S13 which can be used for the formulation of probiotic products.</p></div>","PeriodicalId":7893,"journal":{"name":"Animal Gene","volume":"26 ","pages":"Article 200134"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47906522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of extended Spectrum β-lactamase (ESBL) genes in Escherichia coli isolated from fecal samples of apparently healthy dogs in Ibadan, Nigeria","authors":"Olutayo Israel Falodun,&nbsp;Mobolaji Christianah Afolabi,&nbsp;Akeem Ganiyu Rabiu","doi":"10.1016/j.angen.2022.200133","DOIUrl":"10.1016/j.angen.2022.200133","url":null,"abstract":"<div><p>The incidence of Extended Spectrum β-Lactamases (ESBL) in <em>Escherichia coli</em><span> has generated trepidations because dissemination of ESBL genes constitutes risk to public health. The current study aims to determine antibiotic susceptibility and carriage of ESBL </span><em>bla</em>_SHV, <em>bla</em>_TEM and <em>bla</em>_CTX-M by <em>E. coli</em> isolated from apparently healthy dogs in Ibadan conurbation, Nigeria.</p><p>Freshly passed fecal samples (<em>n</em> = 60) were collected from dogs into sterile sample bottles and processed to isolate <em>E. coli</em> following standard methods. Isolated <em>E. coli</em><span> were confirmed through Polymerase Chain Reaction (PCR) by means of </span><em>uidA</em><span> and tested against 10 antibiotics via Kirby-Bauer disc diffusion technique. </span><em>Escherichia coli</em> showing phenotypic ESBL production were selected for ESBL <em>bla</em>_SHV, <em>bla</em>_TEM <em>bla</em>_CTX-M<span> genotyping using multiplex PCR.</span></p><p>Of the 31 <em>E. coli</em><span> isolates confirmed, 10 (32.3%), 9 (29.0%) and 7 (22.6%) were resistant to ampicillin<span>, tetracycline and trimethoprim/sulfamethoxazole, respectively while 8 (25.8%) were multidrug resistant (MDR). </span></span><em>Escherichia coli</em> isolates (<em>n</em> = 2/8) that were MDR harboured at least one of <em>bla</em>_SHV, <em>bla</em>_TEM and <em>bla</em>_CTX-M genes while another isolate (<em>n</em> = 1) simultaneously possessed the three ESBL genes. Apparently healthy dogs are likely reservoirs of ESBL- <em>E. coli</em> that can spread to pet owners through close contact with their companion animals.</p></div>","PeriodicalId":7893,"journal":{"name":"Animal Gene","volume":"26 ","pages":"Article 200133"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48146599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular identification of ornamental loaches (Cypriniformes, Cobitoidei) of North East India using mitochondrial genes","authors":"Mukunda Goswami ,&nbsp;A. Pavan Kumar ,&nbsp;Grishma S. Patil ,&nbsp;Trinica George ,&nbsp;Rupak Nath ,&nbsp;R.N. Bhuyan ,&nbsp;C. Siva ,&nbsp;M.A. Laskar ,&nbsp;Samson Sumer","doi":"10.1016/j.angen.2022.200136","DOIUrl":"10.1016/j.angen.2022.200136","url":null,"abstract":"<div><p><span>North East (NE) India, referred as Biodiversity Hot Spot, is the natural habitat of a large variety of ornamental fishes<span>. Among all the ornamental fishes available in NE India, Loaches are of great importance in the ornamental fish trade. In this study, 38 specimens from ten loaches species of North-eastern India are characterized using three mitochondrial genes<span> i.e. cytochrome </span></span></span><em>c</em><span> oxidase subunit I (COI), 16S rRNA and cytochrome </span><em>b</em> (Cyt b). The average genetic distance values for all the three mitochondrial genes have increased gradually when moving up from species to genus to family levels. Among the genes, Cyt <em>b</em> gene showed relatively higher genetic distance values than mitochondrial 16S rRNA and COI genes. Some of the specimens of <span><em>Botia</em></span> and <span><em>Lepidocephalichthys</em></span> showed higher intraspecific genetic distance values compared to other specimens under analysis.</p></div>","PeriodicalId":7893,"journal":{"name":"Animal Gene","volume":"26 ","pages":"Article 200136"},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45688875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A β-defensin isoform from the Flathead grey mullet, Mugil cephalus (Linnaeus 1758): Structural and functional characterisation in silico","authors":"P.P. Athira ,&nbsp;V.V. Anooja ,&nbsp;M.V. Anju ,&nbsp;S. Neelima ,&nbsp;K. Archana ,&nbsp;S. Muhammed Musthafa ,&nbsp;Swapna P. Antony ,&nbsp;I.S. Bright Singh ,&nbsp;Rosamma Philip","doi":"10.1016/j.angen.2022.200128","DOIUrl":"10.1016/j.angen.2022.200128","url":null,"abstract":"<div><p><span><span>Defensins are an important class of </span>AMPs<span>, rich in cysteine with antibacterial, antifungal and antiviral functions. The present study identified and functionally characterised a β-defensin gene from Grey mullet, </span></span><span><em>Mugil cephalus</em><em>,</em></span> termed as <em>Mc</em><span><span>-Def. A 192 base pair complementary DNA with an open reading frame encoding 63 </span>amino acids comprising 20 amino acid signal and 43 amino acid mature peptide region was obtained. </span><em>Mc</em><span><span>-Def has a net charge of 3.9 and a molecular weight of 7.27 kDa with an isoelectric point of 8.88. It possessed six cysteines forming 3 </span>disulphide bonds between C</span>¹–C⁵, C²–C⁴ and C³–C⁶<span><span>. Three dimensional structure identified a short N-terminal α helix followed by β sheet strands stabilized by disulfide bonds. The mRNA structure was predicted by Biomodel server with significant MEF score. Multiple </span>sequence alignment and phylogeny revealed the sequence similarity of </span><em>Mc</em>-Def with fish β-defensin 2 type of AMPs. Molecular diversity and evolution of β-defensin provided immunity to host system against various infections. <em>In silico</em><span> analysis identified its potential as antibacterial, antifungal, anticancer, antioxidant and non-hemolytic peptide activity. These intriguing results firmly suggest that the </span><em>Mc</em>-Def can be used as a potent drug molecule in fish health management.</p></div>","PeriodicalId":7893,"journal":{"name":"Animal Gene","volume":"25 ","pages":"Article 200128"},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54099384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A real-time qPCR method for early detection of Bombyx mori Bidensovirus (BmBDV) infection in silkworm","authors":"Gundi Raghavendar ,&nbsp;Tania Gupta ,&nbsp;A. Ramesha ,&nbsp;Vankadara Sivaprasad ,&nbsp;Kangayam M. Ponnuvel","doi":"10.1016/j.angen.2022.200132","DOIUrl":"10.1016/j.angen.2022.200132","url":null,"abstract":"<div><p><span><em>Bombyx mori</em></span> bidensovirus (<em>Bm</em>BDV) replicates in the midgut cells of the silkworm to cause fatal flacherie disease. Flacherie disease causes a major impact in silk production and severe economic loss in the silk industries of India. <em>Bm</em><span>BDV possesses two single stranded DNA<span> that encodes for putative protein-primed DNA polymerase and adopts its own mechanism for replication. Development of rapid, sensitive and simple-to-use novel technology such as real-time polymerase chain reaction (RT-qPCR) will be useful in detection of </span></span><em>Bm</em>BDV in silkworms. This RT-PCR assay will help to understand the significant pathology of <em>Bm</em>BDV. The primers in specific to VD2 ORF1 region of <em>Bm</em>BDV were designed to study their sensitivity and specificity. The detection limit of qPCR was found to be 3.01 × 10¹ copy number in the plasmid DNA with 96.3% efficiency in RT-PCR. In accordance, results shown in our study are 1000 times more sensitive than the conventional PCR with a detection limit of 3 × 10⁴ in PCR assay. Apart from displaying an increased sensitivity, these primers show high specificity towards <em>Bm</em><span>BDV pathogenicity. On detection of flacherie infection studies with the specific primers shows gradual increase in detection of infectivity of </span><em>Bm</em>BDV from 12 h to post infection for 48 h. In this case, the copy number reported at 48 h was found to be &gt;10⁷<span> copies/reaction through qPCR analysis. The validation of this method was conducted using 90 diseased samples collected from 10 different sericulture farms. Here, detected virus copy numbers range from 1.24 × 10</span>³ to 1.08 × 10¹⁰ where the optimization of RT-PCR could be used as a valuable tool in detection of <em>Bm</em>BDV virus. Further aid in formulating appropriate disease control strategies, screening silkworm breeds for resistance during early infection stages are initiating reasons for this study. This particular analysis will provide a platform for developing silkworm hybrids to prevent further crop loss at the field level.</p></div>","PeriodicalId":7893,"journal":{"name":"Animal Gene","volume":"25 ","pages":"Article 200132"},"PeriodicalIF":0.0,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45852186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}